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Plants of the genus MJusa. the bananas and plantains, are large
herbaceous monocotyledons which grow from massive underground
corms. The transition from vegetative to floral growth and subsequent
fruit formation marks the end of growth for an individual shoot meristem. For all rractical purposes, a given shoot behaves like a monocarpic
plant. The first floral organs to form are the bract-covered hands of
female flowers the ovaries of which will become the fruits. In the dessert
bananas (i.e. thcse clones with sweet fruits which are freauenzlv eaten
iaw) and the solled 'French plantains'. the floral apex can continue to
produce large numbes c f :act -covered male flower clusters until the
fruit bunch is harvested (Barker and Steward, 1962; SimnM,. 196. In
those clones designated as 'F]ie Horn' -'antains (with long horn shaped
fruits generally eaten after cooking). the axis of the male inflorescence
terminates in a large single flower and usually whithers away soon after
all of the fruits have formed (De Langhe. 1961; Tezenas du Mcntcel. De
Langhe and Swennen. 1983). Because the dessert and French plantain
clones have. in theory at least, the morphological capacity for continued
formation of primordia on the flanks of their floral apical meristems, it
seems appropriate to refer to such apices as inetera&na-te. Similarly it is
aoropriate to refer to those floral bud types where it seems an apparent
morphologi Cronauer-Mitra and Krikorian cal impossibility to form
additional bract or leaf primordia and buds on the terminal structure as
determinate. Using the dessert banana clone 'Dwarf Cavendish'as a
prototype. we described the means whereby isolated terminal
indeterminate floral apices could be induced to re-initiate a vegetative
growth mode directly with out the formation of an in.ervening callus
(Crunauer and Krikorian. 1985). In this paper. we report that when zhe
distal segment of the determinate inflorescence of a 'False Horn' plantain
is cultured in acvtokinin containing mediun. adventitious vegetative
shoots can be induced to form in the axils of the bractscars. Once this
occurs. the system behaves and can be manipulated like a vegetative
apex-derived culture.
MATERIALS AND METHODS
Terminal flower buds of three 'False Horn' type plantain clones. 'Harton'.
'Hartor Verde'. and 'Harton Negra' of the 'Harton' type [AAB genome
group according to the convention of Simmonds and Shepherd. (1956)]
were collected in Maracay and Santa Barbara del Zulia,Venezuela as
were corms of Harton Verde' and 'Harton Negra' (of. Haddad and Borges.
1973). Flower buds of the common 'False Horn' plantain known in Costa
Rica as 'Curare' were collected in La Lola, Costa Rica. In the case of the
Venezuelan material, the flower buds were 3 Cronauer-Mitra and
Krikorian carried from the field to a laboratory in Caracas. The majority
of the bracts were removed by hand until the buds measured
approximately 6 to 7 cm in length. They were then surface sterilized in a
2% (vv) comercial bleach (Clorox) solution (final concentration 0.1%
(NaOCl) for 5 min and rinsed four times with sterile distilled water. They
were placed on 10 ml of semisolid medium in 18 mm X 150 mm Kaput
tubes (Bellco Glass. inc.. Vineland. N.J.) which were sealed with Parafilm
and hand carried to Stony Brook. The culture medium was composed of
the mineral salts of Murashige and Skoog (1962) and iron of Singh and
Krikorian (1980). 100 mg L inositol. 1 mg L thiamine HCI, 5 mg/L N 6
-henz'aminourine. 4% sucrose, and 0.7% agar. The pH cf the medium
was adjusted to 5.8 with KOH prior to autoclaving at 121 C and 103 kPa
for 20 min. Upon arrival in Stony Biock. the entire flower buds were
placed :n 50 m! of liquid culture medium of the same composition in 250
ml Erlenmever flasks and placed on a gyrotary shaker at 100 rpm in a
growth chamber maintained at 30 C under a 16:8 L:D cycle (10.2 x 103
lmim 2 Sylvania Grolux wide spectrum). The corms were hand carried to
Stony Brook where vegetative apices were isolated as previously
described (Cronauer and Kri korian. 1984, 1986). The Costa Rican
materi.al was hand carried in an insulated cooler from the field to Stony
Brook where they were prepared as described and placed directly into
liquid medium. Multiplying shoot 4 V Cronauer-Mitra and Krikorian
clusters were removed from the floral axis and transferred to semi-solid
medium in 6.5-cm x 6.5-cm x 10-cm plastic vessels (GA 7 vessels from
Magenta Corp.. Chicago, !L) and were routinely subcultured at 3-4 wk
intervals. Roots were induced to form on individual shoots by placing
them on 10 ml of semi-solid medium containing 1 mg L
naphthaleneacetic acid and 0.025% activated charcoal in Kapuz tubes.
Material isolated for histological examination was fixed in Craf III (Berlyn
and Miksche. 1976), embedded in Paraplast. sectioned at 8 um, and
stained
with an aqueous safranin-fast green series. Material was
Fpepared for scanning electron microscopy by fixing in 3% utaraidehyde
in 0.03M PIPES buffer, pH 8.0, at rcom tEnpeiature for 12 to 24 hr. Fixed
specimens were deh':d:ated In a graded ethanol series on ice, critical
point d:ied. mounted on stubs, sputtered with gold and viewed on an
Amray 1000A scanning electron microscope at 5 Kv.