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Genomics
3.1 Introduction to Recombinant DNA
Technology
and DNA Cloning
3.2 What Makes a Good Vector?
3.3 How Do You Identify and Clone a
Gene of
Interest?
3.4 What Can You Do with a Cloned
Gene?
Applications of Recombinant DNA Technology
3.5 Genomics and Bioinformatics:
Hot
New Areas of Biotechnology
Size
Origin of replication (ori)
Multiple cloning site (MCS)
Selectable marker genes
RNA polymerase promoter sequences
DNA sequencing primers
mRNA is degraded
DNA polymerase used to create the second strand of
DNA
Short linker sequences are added to the end of the
cDNA
Contain restriction enzyme recognition sites
Disadvantage
Can be difficult to make the cDNA library if a source tissue with an
abundant amount of mRNA for the gene is not available
Advantage of PCR
Applications