Pseudomonas aeruginosa

Morphology &

Cultural characteristics

Special Features

& Culture media

Slender, Gram negative, Obligate aerobe.

bacilli.

Exotoxin-A

Wound infections

2.

Proteases

Infections

On ordinary media: large, 3.

Elastases

Often piliated

opaque,

Non capsulated, many

colonies.

mucoid slime layer.

Enterotoxin
Hemolysins

infections/post

6.

Slime layer acts as

operative

capsule in enhancing

infections.

On Mac conkey agar:

virulence.

Complications

Lab Diagnosis

Pulmonary complications

Surgical

site

following

Tracheostomy.
wound Infections

Respiratory infections:

with

in

patients

bed

sores/

pressure sores.

Ceftazidime

Pus wound

Cefipime

infections.

Colistin

Sputum

Polymyxin-B

resp:inf

(Specific

Urine UTI

ET

Pili- adhesion

with Cystic fibrosis. On Blood agar : hemolytic 8.

Lipopolysaccharides

b.COPD

9.

Ability to form bio

UTI :

pneumonia in cystic

Utilises

film.

Iatrogenic meningitis

fibrosis patients.

only

Loose

capsules

are

7.

colonies
/hemodigestion

formed /glycocalyx: Oxidative only

bacilli protected from Catalase&oxidase:

Positive.

host defences.

Pigments:

Pyocyaninbluish

green

phenazine,

Conjunctivitis
Severe

complicated

CSF : meningitis
Blood : sepsis
sugars
oxidatively,

Endocarditis

but

Septicaemia.

fermentative.

not

Citrate : positive

Reagent in string test:

TSI K/K

deoxycholate.

sodium

infections )

String test : negative

0.5%

pseudomonas

tubes,

secretions

a.Pneumonia(VAP)

produced in patients

Treatment

Specimen:

Otitis media
patients Diabetic ulcers

with burns

5.

polymers: Non lactose fermenting

Alginate

in

irregular 4.

produce Odor: earthy/ mawkish.

strains

Clinical infections

1.

motile, Opt: temp: 37c

actively

Virulent factors

(Alkaline
slant/alkaline

water soluble,

butt)

diffusible.

Selective medium :

Pyorubin: red

Cetrimide agar for

color

fecal specimens
Reduces Nitrates to
nitrites and then to
gaseous nitrogen.

Amikacin

for