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Animal species:

Technical Bulletin

MONTANIDETM ISA 760 VG


Ready to use adjuvant for veterinary vaccines
TM

Key Points :

Hydrophobic phase made of


lipophilic polymer and biodegradable esters
(Does not contain mineral oil)

MONTANIDE ISA 760 VG is based on synthetic lipophilic polymer and


biodegradable ester mix with an extremely refined emulsifier obtained
from mannitol and purified oleic acid of vegetable origin. It has been
developed for the manufacture of water-in-polymer emulsions.
TM

MONTANIDE ISA 760 VG is free of animal origin ingredients.


TM

Vaccine formulations with MONTANIDE ISA 760 VG induce a strong and long

For stable, fluid, and well

term immunity. Compared to traditional oil emulsions, MONTANIDE


VG emulsions are very stable, well tolerated and easy to inject.

tolerated vaccines

Induces a strong and long


lasting immune response

MONTANIDE listed on Annex

TM

ISA 760

1. VACCINE PREPARATION:
To prepare 100 g of vaccine, a typical composition is recommended:
MONTANIDE ISA 760 VG:
70 g
Aqueous antigenic medium:
30 g

II of the European Council


2377/90/EC
StableRegulation
preparations
are obtained by mixing the aqueous medium into the MONTANIDE
ISA 760 VG, at room
temperature or less, under vigorous stirring. The use of a high shear mixer is necessary to prepare a stable and efficient
vaccine. Adjuvant concentration can be adjusted at 60 % (W/W) to get more room for the antigenic media, while checking
the stability and the injectability of the emulsion.
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2. EMULSION CHARACTERISTICS: (on placebo antigenic medium)

TYPE

VISCOSITY
(mPa.s)

W/P
(water-inpolymer)

About 40
at 25C

CONDUCTIVITY DROPLET SIZE


(S/cm)
(micron)
< 10

1 or less

After storage at
4C
At least 12 months

TYPICAL STABILITY
After storage at
25C

After storage at
37C

At least 6 months

About 15 days

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3. IMMUNE RESPONSE: MONTANIDE ISA 760 VG is capable of improving vaccine efficacy via the induction of a strong
and long lasting immunity. Its a promising adjuvant to stimulate humoral responses. This product is recommended
for bacterial, mycoplasma, viral or parasite antigens.
4. ANIMAL SPECIES:
poultry and fish.

MONTANIDE ISA 760 VG adjuvant is recommended for different vaccines for cattle, swine,

5. STRENGTH: This adjuvant is a flexible and robust formulation. It is adapted to prepare water-in-polymer
emulsions, which induce a strong efficacy similar to oil emulsions. Furthermore it offers the possibility to reduce the
injected dose or to dilute antigenic media with a constant injected volume while keeping the same level of protection.
TM

6. SAFETY AND REGULATORY: The toxicological tests made on MONTANIDE ISA range (Berlin test, Oral LD 50, IP LD 50,
ocular irritation test, dermal irritation test, pyrogenicity) conclude to the safety and favourable tolerance of these
adjuvants.
Montanide adjuvants and their components have been considered as safe by the Committee for Veterinary Medical
Products (CVMP) for use in immunological products and are included as authorized substances in the annex of the
European Council Regulation n 470/2009 (previously 2377/90/EC) needing no further MRL studies, or included in
already registered veterinary commercial products.
The properties of the antigenic media are crucial for the vaccine efficacy but also for its safety. Each team developing a
vaccine will have to study the safety and efficacy profile of the underdevelopment formula according to local market
acceptance criteria.
For more information on this product or for any advice regarding the optimization of your vaccine,
please contact our teams on www.seppic.com or on Montanide@airliquide.com

SEPPIC Inc

33(0) 1 42 91 40 00

1 973 882 5597

Givaudan-Lavirotte SA
33(0) 4 78 61 55 00

SEPPIC China
86(21) 64 66 01 49

www.seppic.com
Subsidiary of the AIR LIQUIDE group

SEPPIC UK Ltd

SEPPIC Italia Srl

44 208 577 8800

39 02 38009110

SEPPIC GmbH
49(0) 220 3890 3100

SEPPIC Belgium NV
32 3 250 3911

3637/GB/01/August 07

SEPPIC SA