Академический Документы
Профессиональный Документы
Культура Документы
DOI 10.1007/s12033-011-9415-5
RESEARCH
Introduction
Jatropha curcas L., belonging to the family Euphorbeaceae, is native to South America and widely distributed in
South and Central America, Africa, and Asia. J. curcas is a
multipurpose shrub with significant economic importance
and has ability to rehabilitate the degraded lands [1]. Since
its seed oil can be converted to biodiesel, it is emerging as a
renewable energy source, alternative to petro-diesel and is
highly promoted for large scale cultivation and production
of biodiesel. Several reports demonstrated better performance of the Jatropha biodiesel over conventional petrodiesel [13]. In spite of high-nutritional composition, seed
cake obtained from the toxic J. curcas remains unutilized
as animal feed due to its toxic nature [4, 5], and no successful attempts have been made till now for completely
eliminating toxic principle [6]. Globally, J. curcas is promoted for large acreage cultivation for biodiesel production
[7, 8]. Selective cultivation of non-toxic variety reported
from Mexico, whose innocuous nature was established
[4, 9, 10], will add value to the crop through utilization of
de-oiled seed cake as a safe animal feed.
123
58
RAPD Analysis
Amplification of RAPD fragments was performed according to Williams et al. [13] using decamer arbitrary primers
123
59
123
60
Fig. 2 Nucleotide sequence of RAPD amplicon specific to non-toxic J. curcas (NT-JC/SCAR I/OPQ15); arrows represent forward primer
(NT-JC/SCAR I/OPL15-F) and reverse primer NT-JC/SCAR I/OPQ15-R), the underlined sequence belongs to the oligo decamer primer OPQ15
Fig. 3 Amplification with NT-JC/SCARI/OPQ15 primer set in nontoxic variety of J. curcas; lanes 14 non-toxic variety J. curcas; lane
5 toxic variety of J. curcas; lane 6 100 bp Marker
123
61
10.
11.
12.
References
1. Ghosh, A., Chaudhary, D. R., Reddy, M. P., Rao, S. N., Chikara,
J., Pandya, J. B., et al. (2007). Prospects for Jatropha methyl ester
(biodiesel) in India. International Journal of Environmental
Studies, 64, 659674.
2. Takeda, Y. (1982). Development study on Jatropha curcas
(Sabudum) oil as a substitute for diesel engine oil in Thailand.
Journal of Agricultural Association of China, 120, 18.
3. Mandpe, S., Kadlaskar, S., Degen, W., & Keppeler, S. (2005). On
road testing of advanced common rail diesel vehicles with biodiesel from the Jatropha curcas plant (vol. 26, pp. 356364).
Society of Automotive Engineering Inc.
4. Makkar, H. P. S., & Becker, K. (1997). Potential of Jatropha seed
cake as protein supplement in livestock feed and constraints to its
utilization. In Proceedings of Jatropha 97: International symposium on biofuel and industrial products from Jatropha curcas
and other tropical oilseed plants, Managua/Nicaragua, Mexico
(pp. 2327).
5. Makkar, H. P. S., Aderibigbe, A. O., & Becker, K. (1998).
Comparative evaluation of non-toxic and toxic varieties of
Jatropha curcas for chemical composition, digestibility, protein
degradability and toxic factors. Food Chemistry, 62, 207215.
6. Martinez-Herrera, J., Sibdhiraju, S., Francis, G., Davila-Ortiz, G.,
& Becker, K. (2006). Chemical composition, toxic/antimetabolic
constituents and effects of different treatments on their levels, in
four provenances of Jatropha curcas L. from Mexico. Food
Chemistry, 96, 8089.
7. Ginwal, H. S., Rawat, P. S., & Srivastava, R. L. (2004). Seed
source variation in growth performance and oil yield of Jatropha
curcas Linn in central India. Silve Senetica, 53, 186192.
8. Sujatha, M., Makkar, H. P. S., & Becker, K. (2005). Shoot bud
proliferation from axillary nodes and leaf sections of non-toxic
Jatropha curcas L. Plant Growth Regulations, 47, 8390.
9. Francis, G., Edingger, R., & Becker, K. (2005). A concept for
simultaneous wasteland reclamation fuel production and socio
economic development in degraded areas in India need potential
13.
14.
15.
16.
17.
18.
19.
20.
21.
123