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Introduction: Orthodontic forces produce a series of changes in dental pulp. However, no one has attempted to
investigate the incidence of pulp necrosis after orthodontic therapy in the clinic. In this study, we aimed to investigate pulp vitality and histologic changes after the application of moderate and severe intrusive forces.
Methods: Twenty-seven adolescent patients were assigned to 1 of 3 groups: the control group of 3 subjects;
the moderate-force group, with 12 subjects who received a 50-g force to the rst premolars bilaterally; and
the severe-force group, with 12 subjects who received a 300-g force. The forces were applied for 1, 4, 8, or
12 weeks. An electric pulp tester was used to test for vitality, and teeth that did not respond to the electric
pulp tester were subsequently tested thermally with a stick of heated gutta-percha. Results: The teeth with a
negative response to the electric pulp tester still responded to the thermal test. We found odontoblast disruption,
vacuolization, and moderate vascular congestion in both force groups, but no necrosis was observed. Pulp
stones were formed only in the severe-force group. Conclusions: Dental pulp still has vitality after intrusive
treatment with different forces. These data provide new insights into the effects of intrusive orthodontic forces.
(Am J Orthod Dentofacial Orthop 2013;144:518-22)
a
Attending physician, Department of Endodontics, School of Stomatology, Jilin
University, Changchun, China.
b
Professor, Department of Orthodontics, School of Stomatology, Jilin University,
Changchun, China.
c
Master student, Department of Orthodontics, School of Stomatology, Jilin
University, Changchun, China.
d
PhD student, Department of Orthodontics, School of Stomatology, Jilin
University, Changchun, China.
All authors have completed and submitted the ICMJE Form for Disclosure of Potential Conicts of Interest, and none were reported.
Supported by fund from the Natural Science Foundation of China (811709999/
H1409).
Reprint requests to: Min Hu, Department of Orthodontics, School of Stomatology, Jilin University, No 1500, Qinghua Rd, Changchun 130021, Jilin, China;
e-mail, ydhhm@sohu.com.
Submitted, January 2013; revised and accepted, May 2013.
0889-5406/$36.00
Copyright 2013 by the American Association of Orthodontists.
http://dx.doi.org/10.1016/j.ajodo.2013.05.005
518
This study was conducted on 54 maxillary rst premolars collected from 27 prospective orthodontic patients (15 male, 12 female) with a mean age of 17.9
years (range, 14-24 years) who required rst premolar
extractions. The 27 subjects were randomly divided
into 3 groups: the control group without orthodontic
forces (3 subjects), the moderate-force group (12
Han et al
519
Fig 1. The experimental setting: A, the appliance used to apply buccally directed forces on both rst
premolars; B, the transpalatal arch was disengaged from occlusal contact.
Han et al
520
Table. Comparison of electric pulp tester (EPT) readings between the moderate-force and severe-force groups at the
observation (means 6 standard deviations)
Moderate-force group
Week
0
1
2
3
4
5
6
7
8
9
10
11
12
Teeth (n)
24
6
6
6
6
6
6
6
6
6
6
6
6
Severe-force group
EPT reading
3.44 6 2.37
4.50 6 2.56
5.20 6 2.57y
4.29 6 2.36
6.43 6 2.07y
7.68 6 0.35z
8.35 6 0.38z
8.67 6 0.43z
0
0
0
0
0
Teeth (n)
24
6
6
6
6
6
6
6
6
6
6
6
6
EPT reading
5.33 6 2.45
6.63 6 1.77
7.20 6 0.84
7.67 6 0.58
0
0
0
0
0
0
0
0
0
t
1.900
2.10
2.23
3.55
P
0.07
0.047*
0.045*
0.009*
*P\0.05 compared with the corresponding moderate-force group; yP\0.05 compared with the readings at baseline (week 0); zP\0.001 compared
with the readings at baseline (week 0).
to the electric pulp tester, there were signicant differences between the moderate-force and severe-force
groups at weeks 1, 2, and 3 (P \0.05). Readings of the
moderate-force group at weeks 2 and 4 were signicantly increased compared with those at baseline
(P \0.05). In addition, there were signicant differences
between the readings of the moderate-force group at
weeks 5, 6, and 7 and those at baseline (P \0.001). After
4 weeks, pulp vitality measurements became signicantly different from those during the previous weeks
(P 5 0.009). Readings of the electric pulp tester
increased over time in the severe-force group, but
showed no statistical difference (t 5 3.799; P 5 0.06).
In the control group, we observed a columnar odontoblast cell layer with normal morphology. A cell-free
zone was obvious, and no inammatory cell inltration
or vascular congestion was observed (Fig 2, A).
At week 1, the odontoblast nuclei in the odontoblast
zone in both force groups appeared abnormal, and vacuolization in the odontoblast layer and moderate
vascular congestion were observed. The structure of the
cell-free zone was disrupted in the severe-force group.
No signicant change was observed in the root pulp.
At week 4, in the coronal pulp of the experimental
groups, vacuolization and moderate vascular congestion
were observed. The arrangement of the odontoblast cell
layers, the cell-free zones, the vessels, and the amounts
of pulpal cells were similar to those in the control group.
In addition, the extent of vacuole formation was worse
in the severe-force group than in the moderate-force
group. The resorption of craters in the root with some
cementum deposition was obvious in the severe-force
group (Fig 2, B).
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521
Fig 2. Histology of the teeth in the different groups, with representative pictures from multiple teeth in
each group. A, Normal morphology in the control group; D, Dentin; O, odontoblast layer; CFZ, cell-free
zone; CRZ, cell-rich zone; V, vessels; hematoxylin and eosin (HE) staining; magnication, 100 times.
B, At week 4, root resorption was obvious with some cementum deposition in the severe-force group;
HE staining; magnication, 100 times. C, At week 8, several pulp stones were found in the root pulp in
the severe-force group; HE staining; magnication, 100 times. D, At week 12, root resorption appeared
in the moderate-force group; HE staining; magnication, 40 times. E, At week 12, vacuolization in the
odontoblastic layer, adipose degeneration of the pulp cells, and inammatory extravasation were
observed in the severe-force group; HE staining; magnication, 200 times. F, At week 12, vascular dilatation such as arborization and extravasation of red blood cells into pulp tissue, and vascular congestion were observed in the coronal pulp of the severe-force group; HE staining; magnication, 100 times.
G, At week 12, calcic lines were observed around the dentin in the roots of teeth in the severe-force
group; HE staining; magnication, 200 times. H, At week 12, inammatory cell inltration and vascular
congestion were observed around the vessels in the moderate-force group; HE staining; magnication,
100 times.
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