Recovery of Human Mesenchymal Stem Cells Following Cryopreservation With

Serum-Free CryoStor
Comparison to Serum Containing Home-Brew Media
125

% Recovery of Viable Cells

100

75

50

25

0
37C

CryoStor
CS5

95% Serum
*

FBM
Medium

FGM
Medium *

Normosol Normosol * Plasma-lyte Plasma-lyte

*

5% DMSO

alamarBlue stain for metabolic activity

* Contains serum

Recovery of Normal Human Dermal Fibroblasts Following Cryopreservation

With Serum-Free CryoStor
Commercial Solution Comparison: Serum and Serum-Free
125

% Recovery of Viable Cells

100

75

50

25

0
Control
(37C)

CryoStor ProFreeze
CS5

Aedesta

Cellvation

Cell
Guardian

Synth-a- Recovery * Sigma

Freeze
Freeze *

alamarBlue stain for metabolic activity

FGM + 5%
DMSO *

* Contains serum

Representative Fluorescent Micrographs Illustrating Post Cryopreservation Morphology of

NHDF Stored in Serum-Free CryoStor

CryoStor CS5

ProFreeze

Cell Guardian

Synth-a-Freeze

Green
Actin Cytoskeleton
Phalloidin (FITC)

Aedesta

Recovery

Red
Mitochondria Activation
MitoTracker Red

Cellvation

Sigma

Blue
Nuclear Stain
Hoechst

Recovery of Human Mesenchymal Stem Cells Following Cryopreservation With

Serum-Free CryoStor
Comparison to Serum Containing Home-Brew Media
125

% Recovery of Viable Cells

100

75

50

25

0
Control

CS2

CS5
CryoStor

CS10

FGM + 2%
DMSO *

FGM + 5%
DMSO *

FGM + 10%
DMSO *

Culture Media
alamarBlue stain for metabolic activity

* Contains serum

Representative Fluorescent Micrographs Illustrating Post Cryopreservation Morphology of

hMSC Stored in Serum-Free CryoStor

CryoStor CS2

CryoStor CS5

CryoStor CS10

FGM + 2% DMSO

FGM + 5% DMSO

FGM + 10% DMSO

Green
Actin Cytoskeleton
Phalloidin (FITC)

Red
Mitochondria Activation
MitoTracker Red

Blue
Nuclear Stain
Hoechst

Recovery of Human Mesenchymal Stem Cells Following Cryopreservation With

Serum-Free CryoStor
Comparison to Serum Containing Home-Brew Media
125

% Recovery of Viable Cells

100

75

50

25

0
37C

CryoStor 90% Serum MSCGM

CS10
*
(Medium)
(no serum)

MSCGM
Normosol
(Medium) *

Normosol * Plasma-lyte Plasma-lyte

*

10% DMSO

alamarBlue stain for metabolic activity

* Contains serum

Representative Fluorescent Micrographs Illustrating Post Cryopreservation

Differentiation Capacity and Morphology of hMSC Stored in CryoStor

hMSC (Undifferentiated)

hMSC Osteoblasts

Green
Actin Cytoskeleton
Phalloidin (FITC)

Red
Mitochondria Activation
MitoTracker Red

Blue
Nuclear Stain
Hoechst

Recovery of hMSC Differentiated Osteoblast Cells Following Cryopreservation

With Serum-Free CryoStor
Comparison With Serum Containing Home-Brew Media
125

% Recovery of Viable Cells

100

75

50

25

0
Control

CS2

CS5

CS10

MSCGM + 2% MSCGM + 5% MSCGM + 10%

DMSO *
DMSO *
DMSO *

CryoStor

Media

alamarBlue stain for metabolic activity

* Contains serum

Recovery of Human Dental Pulp Stem Cells Following Cryopreservation with

Serum-Free CryoStor
Comparison to Serum Containing Home-Brew Media
125

% Recovery of Viable Cells

100

75

50

25

0
37C

CryoStor 90% Serum

RPMI
CS10
*
(Medium)
(no serum)

RPMI
Normosol
(Medium) *

Normosol * Plasma-lyte Plasma-lyte

*

10% DMSO

alamarBlue stain for metabolic activity

* Contains serum