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D Alert # 17

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2015/08/11
Created by
Atsushi Nakajima
(@Ats_Nakajima)

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D Alert #

17

20150811-1

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Four new species in Leucoagaricus (Agaricaceae, Basidiomycota) from Asia

Leucoagaricus4

Bibliographic information
ZW Ge, T Qasim, ZL Yang, R Nawaz, AN Khalid - , 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-351.abstract

Abstract/Summary
Abstract The genus Leucoagaricus has been well studied in Europe. However, species diversity of Leucoagaricus in Asia remains poorly
known, especially in the mountains of southwestern China, a hot spot for biodiversity. Based on morphological characters and molecular
data, four new species are described, La. asiaticus, La. subcrystallifer, La. subpurpureolilacinus and La. truncatus. Detailed morphological
descriptions, drawings of microstructures for novel taxa and comparisons with closely allied taxa are provided. Phylogenetic analyses
inferred from internal transcribed spacer (ITS) and region 6-7 of the gene for the second largest subunit of RNA polymerase II (rpb2)
sequences show that the novel taxa are nested within a well-supported clade jointly formed by members of Leucoagaricus section
Rubrotincti and subgenus Sericeomyces.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/China, /section, /mountains,
/new species

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D Alert #
20150811-2

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Revisiting the morphology and phylogeny of Lactifluus with three new lineages from
southern China

Lactifluus3

Bibliographic information
X Wang, B Buyck, A Verbeken, K Hansen - Mycologia, 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/13-393.abstract

Abstract/Summary
Abstract As a recent group mainly defined by molecular data the genus Lactifluus is in need of further study to provide insight into the
morphological and molecular variation within the genus, species limits and relationships. Phylogenetic analyses of nuc rDNA ITS1-5.8S-ITS2
(ITS), D1 and D2 domains of nuc 28S rDNA (28S), and part of the second largest subunit of the RNA polymerase II (rpb2) (6-7 region)
sequences of 28 samples from southern China revealed three new lineages of Lactifluus. Two of them are nested in a major clade that
includes the type of Lactifluus and here is treated as two new sections: L. sect. Ambicystidiati and L. sect. Tenuicystidiati. Lactifluus
ambicystidiatus, described here as a new species (= sect. Ambicystidiati), has both lamprocystidia and macrocystidia in the hymenium, a
unique combination of features within Russulaceae. Furthermore, only remnants of lactiferous hyphae are present in L. ambicystidiatus and
our results suggest that the ability to form a lactiferous system has been lost in this lineage. Lactifluus sect. Tenuicystidiati forms a strongly
supported monophyletic group as a sister lineage to L. sect. Lactifluus. We recognize it based on the thin-walled macrocystidia and smaller
ellipsoid spores with an incomplete reticulum compared with L. sect. Lactifluus. The former placement of L. tenuicystidiatus in the African L.
sect. Pseudogymnocarpi is not supported. Using genealogical concordance we recognize five phylogenetic species within L. sect.
Tenuicystidiati and describe two of these as new, L. subpruinosus and L. tropicosinicus. The third lineage, represented by L. leoninus, forms
a sister group to L. subg. Lactariopsis sensu stricto. The three lineages provide further evidence for morphological features in Lactifluus
being homoplasious. Some sections and species complexes are likely to be composed of more species and merit further investigations.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Russulaceae, /China,
/new species

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D Alert #
20150811-3

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Perichaena longipes, a new myxomycete from the Neotropics

Perichaena longipes

Bibliographic information
LM Walker, D Leontyev, SL Stephenson - Mycologia, 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-330.abstract

Abstract/Summary
Abstract A new species of myxomycete, Perichaena longipes, is described from 56 specimens of fruiting bodies that appeared in moist
chamber cultures prepared with samples of decaying plant materials collected in Panama, Costa Rica and Brazil. This new species is
distinguished from the morphologically similar species P. pedata on the basis of the much longer stipe, lighter peridium and the unique
ornamentation of the capillitium. The nuc 18S ribosomal DNA sequences obtained from four specimens of P. longipes support the distinction
of this new taxon and its separation from P. pedata. Furthermore, maximum likelihood phylogeny supports earlier evidence that species
currently within the genus Perichaena do not form a monophyletic clade. Instead they appear to form three separate branches within the
bright-spored clade. The first clade includes P. longipes together with several species of Trichia and Metatrichia, the second includes P.
pedata and P. chrysosperma, and the third clade is composed of P. corticalis, P. depressa and P. luteola.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Costa Rica, /Panama,
/Brazil, /new species

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D Alert #
20150811-4

17

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

A critical revision of the Tubifera ferruginosa complex

Tubifera ferruginosa

Bibliographic information
D Leontyev, M Schnittler, SL Stephenson - Mycologia, 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-271.abstract

Abstract/Summary
Abstract Based on a combination of morphological and molecular investigations, a critical revision of the widely distributed myxomycete
Tubifera ferruginosa is presented. A phylogeny of the morphospecies, based on partial 18S nuc rDNA sequences, displays several clearly
distinct clades, all differing by a genetic distance (p distance) of at least 0.15, with the distance within the clades below 0.11. These
molecular differences correlate with morphological characters, such as the shape of sporothecal tips, the color of immature fructifications
and the ultrastructure of the inner surface of the peridium. The combination of morphological and molecular data provides evidence that T.
ferruginosa is actually a species complex, representing at least seven species. These are T. ferruginosa sensu stricto, T. applanata, T.
corymbosa, T. dudkae, T. magna, T. montana and T. pseudomicrosperma. Among these T. applanata and T. dudkae (as Reticularia dudkae)
were described recently based on morphological characters and the 18S nuc rDNA phylogeny confirmed their separation. Another four
species, T. corymbosa, T. magna, T. montana and T. pseudomicrosperma, are described here. We propose an epitype for T. ferruginosa
sensu stricto and recognize subsp. ferruginosa and subsp. acutissima within this species. All studied taxa of the T. ferruginosa complex are
shown to lack a capillitium. Structures formerly described as capillitium represent the hyphae of fungi occurring within the fructifications.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/species complex,
/ultrastructure

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D Alert #
20150811-5

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Ceratocystis tiliae sp. nov., a wound pathogen on Tilia americana

Tilia americanaCeratocystis tiliae

Bibliographic information
L Oliveira, TC Harrington, R Freitas, D McNew - , 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-273.abstract

Abstract/Summary
Abstract Species in the North American clade (NAC) of the Ceratocystis fimbriata complex are mostly weak pathogens that infect native tree
hosts through fresh wounds. Isolations from discolored tissue of wounded Tilia americana (basswood) in Iowa and Nebraska yielded a
Ceratocystis species that was similar to but distinct from isolates of C. variospora from other hosts. Sequences of 28S rDNA showed that
isolates from basswood did not differ from C. variospora, but there were minor differences in ITS rDNA sequences. The DNA sequences of a
portion of the Cerato-platanin gene and TEF1 showed the basswood fungus to be a unique lineage. Cross inoculations in two experiments
showed that the basswood isolates and C. variospora isolates from Quercus spp. were most aggressive to their respective hosts. Isolates
from basswood grew slower and were less pigmented than C. variospora isolates from Quercus spp. The basswood fungus thus is
distinguished from C. variospora based on phylogenetic analyses and phenotype and is herein described as C. tiliae sp. nov.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Iowa, /Quercus,
/Tilia, /Nebraska

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D Alert #
20150811-6

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Umbelopsis longicollis comb. nov. and the synonymy of U. roseonana and U.


versiformis with U. nana

Umbelopsis longicollisU. roseonanaU. versiformisU. nana

Bibliographic information
YN Wang, XY Liu, RY Zheng - Mycologia, 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-339.abstract

Abstract/Summary
Abstract Based on maximum growth temperatures, morphological characteristics and multilocus phylogenies (partial 18S and 28S, internal
transcribed spacer regions of nuc rDNA and a fragment of an actin gene), Umbelopsis roseonana and U. versiformis are treated as
heterotypic synonyms of U. nana, while the phylogenetically closely related U. dimorpha is retained as a separate species. Mortierella
longicollis is reclassified as a new combination, U. longicollis. In addition, lectotypes and epitypes for the basionyms of U. longicollis and U.
nana are designated here.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/new combination

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D Alert #
20150811-7

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Lignomyces, a new genus of pleurotoid Agaricomycetes

Lignomyces

Bibliographic information
RH Petersen, NV Psurtseva, IV Zmitrovich - , 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-355.abstract

Abstract/Summary
Abstract Collections of a pleurotoid fungus from dead aspen in eastern Russia were initially identified as Lentinus sp., then as Phyllotopsis
nidulans. DNA sequencing of cultures derived from these specimens using the nuclear ribosomal 28S (nrLSU) and nuclear ribosomal ITS1
-5.8S-ITS2 regions (nrITS) showed that they were neither Lentinus nor Phyllotopsis and were not related to other pleurotoid genera
Hohenbuehelia and Pleurotus. Subsequent investigation showed that the Russian fungus was the same as Pleurotus vetlinianus described
from Poland. A new genus, Lignomyces, is described and characterized and L. vetlinianus comb. nov. is proposed.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Poland, /Russia,
/new genus, /sequencing

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D Alert #

17

20150811-8

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Hymenochaetaceae from the Guineo-Congolian rainforest: three new species of


Phylloporia based on morphological, DNA sequences and ecological data

-DNA
Phylloporia3

Bibliographic information
P Yombiyeni, A Balezi, M Amalfi, CA Decock - Mycologia, 2015 - Mycol Soc America

URL
http://www.mycologia.org/content/early/2015/06/26/14-298.abstract

Abstract/Summary
Abstract Four species are added to Phylloporia. Three species, originating from the western edge of the Guineo-Congolian rainforest in
Gabon (central Africa), are described as new. Phylloporia afrospathulata sp. nov. forms seasonal, stipitate, solitary basidiomata emerging
from soil, more likely connected to buried roots, and has broadly ellipsoid basidiospores. Phylloporia inonotoides sp. nov. forms seasonal
sessile, soft basidiomata, solitary at the base of small-stemmed trees including Crotonogyne manniana (Euphorbiaceae) and Garcinia cf.
smeathmannii (Clusiaceae). It has a homogeneous context, large pores (2-3 mm), and oblong-ellipsoid to suballantoid basidiospores.
Phylloporia fulva sp. nov. forms sessile, conchate, mostly pendant, gregarious basidiomata emerging from the trunk of an unidentified
small-stemmed tree and has small, subglobose basidiospores. This species is compared to Polyporus pullus and Phylloporia pulla comb. nov.
and proposed based on the study of the type specimen. Phylogenetic inferences using partial nuc 28S DNA sequence data (region including
the D1/D2/D3 domains) and the most exhaustive dataset available to date resolved these new morphospecies as three distinct terminal
lineages. No sequence data of P. pulla currently is available. The 28S-based phylogenic inferences poorly resolved the interspecific
relationships within the Phylloporia clade.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Gabon,
/Hymenochaetaceae,
/Polyporus, /Euphorbiaceae,
/Garcinia, /Clusiaceae,
/new species

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D Alert #

17

20150811-9

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2015/08/11

Ecological roles of saprotrophic Peronosporales (Oomycetes, Straminipila) in natural


environments

Bibliographic information
AV Marano, AL Jesus, JI de Souza, GH Jernimo - Fungal Ecology, 2015 - Elsevier

URL
http://www.sciencedirect.com/science/article/pii/S1754504815000811

Abstract/Summary
Abstract The fungus-like Peronosporales are composed of several lineages of mainly biotrophic and hemibiotrophic representatives.
Saprotrophic species of Peronosporales are limited to the genera Halophytophthora and Salisapilia, and to some species in Phytopythium
and Phytophthora Clades 6 and 9, which inhabit terrestrial and all types of aquatic ecosystems. The recent discovery of species of
Phytophthora in marine habitats and of Halophytophthora in freshwater indicated that these genera are not only morphologically but also
ecologically poorly delineated. In addition, half of these genera are not monophyletic. They play key ecological roles by upgrading nutrients
to higher trophic levels through colonization of plant debris, which makes substrata more palatable for detritivores or through zoospore
grazing by zooplankton, although their role as saprotrophs is still largely neglected. Some species of Phytophthora can be aggressive
opportunistic pathogens of riverine forests in the presence of susceptible hosts and favourable environmental conditions and, as a
consequence, most studies have focused on their role as pathogens. Identification of species is challenging due to hybridization and species
complexes that harbour multiple cryptic species and, therefore, is not reliable without DNA sequencing tools.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Oomycetes, /freshwater,
/sequencing, /cryptic species,
/hybridization

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D Alert #
20150811-11

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

One hundred million year old ergot: psychotropic compounds in the Cretaceous?

Bibliographic information
G POINAR JR, S ALDERMAN, J WUNDERLICH - palaeodiversity.org

URL
http://www.palaeodiversity.org/pdf/08/02Palaeodiversity_8-15_Poinar-et-al_1.pdf

Abstract/Summary
A fungal sclerotium similar to those of the genus Claviceps, commonly known as ergot, was discovered infect-ing a grass floret in Earlymid
Cretaceous Myanmar amber. The fungus, described as Palaeoclaviceps parasiticus gen. n., sp. n. is characterized by an erect black
sclerotium with flattened hyphal outgrowths and adjacent conidia consistent in morphology with those of extant species of Claviceps. The
lobed hyphal outgrowths on the distal end of the sclerotium separate the fossil from all extant species of Claviceps as well as other
sclerotium- producing fungi in the Clavicipitaceae. The fossil shows that the original hosts of the Clavicipitaceae could have been grasses and
establishes the presence of the Clavicipitaceae and Poaceae in the Earlymid Cretaceous.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Poaceae, /Myanmar,
/amber, /Cretaceous,
/sclerotium, /ergot

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D Alert #
20150811-12

17

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

New species and records of mycosphaerellaceous fungi from living fern leaves in East
Asia

Bibliographic information
R Kirschner, H Wang - Mycological Progress, 2015 - Springer

URL
http://link.springer.com/article/10.1007/s11557-015-1085-4

Abstract/Summary
Six species of mycosphaerellaceous fungi on ferns from mainland China and Taiwan island are investigated by morphology and analysis of
the internal transcribed spacer of the ribosomal RNA genes. Two species of Pseudocercospora from spots on living leaves of ferns
(Polypodiales) in Taiwan are newly described. One species found on Microlepia spp. (Dennstaedtiaceae) differs from Ps. lonchitidis, the
single other species considered as occurring on members of the Dennstaedtiaceae, by narrower conidiophores and conidia. Another species
found on Tectaria harlandii (Tectariaceae) differs from the majority of Pseudocercospora species by the internal hyphae which are often
thick-walled and/or covered by conspicuous incrustations, and from the single other species known from the same host family, Ps.
helminthostachydis, by narrower conidiophores and conidia. Mycosphaerella gleicheniae, Periconiella lygodii, Ps. lygodii, and Zasmidium
dicranopteridis are recorded for the first time from mainland China and Ps. pteridicola from Taiwan. Based on new collections from both
regions and the type, Pseudocercospora lygodii is transferred to Passalora.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Dennstaedtiaceae,
/China, /Taiwan

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D Alert #
20150811-18

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Characterization of Colletotrichum fructicola, a new causal agent of leaf black spot


disease of sandy pear (Pyrus pyrifolia)

Colletotrichum fructicola

Bibliographic information
PF Zhang, LF Zhai, XK Zhang, XZ Huang - European Journal of , 2015 - Springer

URL
http://link.springer.com/article/10.1007/s10658-015-0715-7

Abstract/Summary
In recent years, a devastating fungal disease characterized by small black spots (<1 mm) on sandy pear (Pyrus pyrifolia Nakai) leaves has
occurred in sudden outbreaks in southern China, resulting in severe defoliation and a loss of fruit quality and yield. To identify the etiology of
the disease, 147 fungal isolates from sandy pear leaves showing typical black spots were collected from 30 orchards; 10 fungal species were
included among the isolates, and the prevailing 124 Colletotrichum isolates were subjected to morphological and molecular characterization.
Based on differences in colony morphology, the isolates were separated into five groups (I to V); the groups were specifically separated
according to a combination of colony color, pigment, sporulation, perfect stage, and spore size. In addition to their morphological features,
the five groups were assessed for their molecular and taxon statuses based on ITS sequences, multilocus regions (ITS, ACT, TUB, CHS-1,
GAPDH), and the Apn2/MAT locus, which provided molecular proof for the identification of these isolates as Colletotrichum fructicola
Prihastuti. The result also indicated different resolution of the three loci in delimiting the species. Kochs postulates were fulfilled by
inoculating conidium suspensions of the representative isolates on attached and detached leaves of sandy pear cvs. Cuiguan, Xueqing and
Huali No. 2 with analogous conditions (under saturated humidity and with tiny wounds) to those that appeared in the field, and similar
symptoms were elicited by the isolates, suggesting that C. fructicola was causing the disease. To our knowledge, this is the first report of C.
fructicola causing leaf black spot of pear. The etiological clarification and characterization of the C. fructicola isolates provide useful
information to aid in the understanding of leaf black spot disease in pears and for designing management strategies to control this

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Pyrus, /China, /spot
disease, /humidity, /black spot

Daikinrin Daily Document Alert

D Alert #
20150811-19

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Rust Diseases on Switchgrass (Panicum virgatum)

Panicum virgatum

Bibliographic information
Y Ma - 2015 - digitalcommons.unl.edu

URL
http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1093&context=agronhortdiss

Abstract/Summary
In recent years, a devastating fungal disease characterized by small black spots (<1 mm) on sandy pear (Pyrus pyrifolia Nakai) leaves has
occurred in sudden outbreaks in southern China, resulting in severe defoliation and a loss of fruit quality and yield. To identify the etiology of
the disease, 147 fungal isolates from sandy pear leaves showing typical black spots were collected from 30 orchards; 10 fungal species were
included among the isolates, and the prevailing 124 Colletotrichum isolates were subjected to morphological and molecular characterization.
Based on differences in colony morphology, the isolates were separated into five groups (I to V); the groups were specifically separated
according to a combination of colony color, pigment, sporulation, perfect stage, and spore size. In addition to their morphological features,
the five groups were assessed for their molecular and taxon statuses based on ITS sequences, multilocus regions (ITS, ACT, TUB, CHS-1,
GAPDH), and the Apn2/MAT locus, which provided molecular proof for the identification of these isolates as Colletotrichum fructicola
Prihastuti. The result also indicated different resolution of the three loci in delimiting the species. Kochs postulates were fulfilled by
inoculating conidium suspensions of the representative isolates on attached and detached leaves of sandy pear cvs. Cuiguan, Xueqing and
Huali No. 2 with analogous conditions (under saturated humidity and with tiny wounds) to those that appeared in the field, and similar
symptoms were elicited by the isolates, suggesting that C. fructicola was causing the disease. To our knowledge, this is the first report of C.
fructicola causing leaf black spot of pear. The etiological clarification and characterization of the C. fructicola isolates provide useful
information to aid in the understanding of leaf black spot disease in pears and for designing management strategies to control this

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Panicum, /Pyrus, /China,
/spot disease, /humidity,
/black spot

Daikinrin Daily Document Alert

D Alert #
20150811-20

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Mycological and palynological studies of early medieval cultural layers from


strongholds in Pszczew and Santok (western Poland)

PszczewSantok

Bibliographic information
K Mazurkiewicz-Zapaowicz - Acta , 2015 - pbsociety.org.pl

URL
https://pbsociety.org.pl/journals/index.php/am/article/view/am.1059

Abstract/Summary
Abstract Cultural layers from early medieval strongholds in Pszczew and Santok have been examined for the presence of pollen grains and
spores as well as residues of fungi. The presence of the following remains has been recorded: fossil hyphopodia of Gaeumannomyces,
teliospores of Puccinia, spores of Bipolaris, Thecaphora and Tilletia, teliospores of the genus Urocystis, Ustilago and Uromyces, ascocarps
(perithecium) of the Ascomycota or the pycnidium of Sphaeropsidales. A greater diversity and abundance of fungi spores sensu lato was
recorded in Santok, as compared to Pszczew. Both early medieval sites recorded a significant proportion of cereal pollen, including Secale
cereale. It remains an undisputed fact that the grains and other plants collected in both strongholds were strongly infected with fungi. The
analysis of the cultural layers for the presence of fungi remains provides significant data on the presence of certain species of plants and
their growth conditions in natural environments and in agriculture.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Poland, /Secale

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D Alert #
20150811-23

17

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Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform


Rust in Loblolly Pine (Pinus taeda L.)

Fusiform Rust9

Bibliographic information
HV Amerson, CD Nelson, TL Kubisiak, EG Kuhlman - Forests, 2015 - mdpi.com

URL
http://www.mdpi.com/1999-4907/6/8/2739

Abstract/Summary
Abstract Nearly two decades of research on the host-pathogen interaction in fusiform rust of loblolly pine is detailed. Results clearly indicate
that pathotype-specific genes in the host interacting with pathogen avirulence cause resistance as defined by the non-gall phenotype under
favorable environmental conditions for disease development. In particular, nine fusiform rust resistance genes (Fr genes) are described here
including the specific methods to determine each and their localization on the reference genetic map of loblolly pine. Understanding how
these and other apparent Fr genes in loblolly pine and other rust-susceptible pines impact resistance screening, parental and progeny
selection, and family and clonal deployment is an important area in forest genetics research and operational tree breeding. The
documentation of these Fr genes is a key piece of information towards gaining that understanding and ultimately improving breeding and
deployment strategies.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/screening, /Pinus,
/forest

Daikinrin Daily Document Alert

D Alert #
20150811-29

17

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

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2015/08/11

Dieback of cocoa (Theobroma cacao L.) plant tissues caused by the brown cocoa
mirid Sahlbergella singularis Haglund (Hemiptera: Miridae) and associated pathogenic
fungi

Sahlbergella singularis

Bibliographic information
JC Anikwe, HA Otuonye - International Journal of Tropical Insect - Cambridge Univ Press

URL
http://journals.cambridge.org/abstract_S1742758415000120

Abstract/Summary
The brown cocoa mirid, Sahlbergella singularis is the most important insect pest of cocoa for field damage has been classified into three
categories that include blast, stagheadedness and mirid pocket. The mirid attack predisposes the cocoa plant to secondary infection by
opportunistic pathogenic fungi. This study evaluated the dieback conditions associated with mirid damage due to associated fungi from
lesions on pods, twigs and pin punctures. The relationship between Phytophthora black pod infection and mirid infestation in the field was
also established. The results indicated that dieback progression from the mirid lesions (8.5 mm) was significantly different (P< 0.05) from
that of pin punctures (7.8 mm). Lasiodiplodia theobromae had the highest proportion of isolates from pods (33%), twigs (47%), and pin
punctures (38%). Fusarium decemcellulare (Albonectria rigidiuscula) accounted for 27.8% and 31.6% from pods and twigs; however, it was
not recovered from pin punctures. Other isolated pathogens were Aspergillus species and Rhizoctonia species. There was a significantly
strong positive correlation coefficient of 0.74 between the mirid population and the black pod disease infection in the field. The major fruitbearing season of cocoa witnessed an increase in mirid infestation and blackpod infection. The effect of miridpathogenic fungi-associated
damage could be ameliorated through effective crop husbandry practices to ensure that cocoa plant tissues are not unduly exposed to
biological and mechanical injuries.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Theobroma, /Miridae,
/pathogenic fungi

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Long-term warming alters richness and composition of taxonomic and functional


groups of arctic fungi

Bibliographic information
J Geml, LN Morgado, TA Semenova - FEMS , 2015 - femsec.oxfordjournals.org

URL
http://femsec.oxfordjournals.org/content/early/2015/08/06/femsec.fiv095.abstract

Abstract/Summary
Abstract Fungi, including symbionts, pathogens and decomposers, play crucial roles in community dynamics and nutrient cycling in
terrestrial ecosystems. Despite their ecological importance, the responses of most arctic fungi to climate warming is unknown, so are their
potential roles in driving the observed and predicted changes in tundra communities. We carried out deep DNA sequencing of soil samples to
study the long-term effects of experimental warming on fungal communities in dry heath and moist tussock tundra in Arctic Alaska. The
data presented here indicate that fungal community composition responds strongly to warming in the moist tundra, but not in the dry
tundra. While total fungal richness were not significantly affected by warming, there were clear correlations among OTU richness of various
ecological and taxonomic groups and long-term warming. Richness of ectomycorrhizal, ericoid mycorrhizal and lichenized fungi generally
decreased with warming, while richness of saprotrophic, plant and animal pathogenic, and root endophytic fungi tended to increase in the
warmed plots. More importantly, various taxa within these functional guilds followed opposing trends that highlight the importance of
species-specific responses to warming. We recommend that species-level ecological differences are taken into account in climate change and
nutrient cycling studies that involve arctic fungi. Alaska climate change ITEX ITS metabarcoding Toolik Lake FEMS 2015. All rights
reserved. For permissions, please e-mail: journals.permissions@oup.com

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Alaska, /tundra,
/Arctic, /climate change,
/sequencing

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2015/08/11

Tree Species Composition Predicts Epiphytic Lichen Communities in an African


Montane Rain Forest

Bibliographic information
A Frisch, J Rudolphi, D Sheil, A Caruso, G Thor - Biotropica, 2015 - Wiley Online Library

URL
http://onlinelibrary.wiley.com/doi/10.1111/btp.12237/full

Abstract/Summary
Abstract The ecology of many tropical rain forest organisms, not the least in Africa, remains poorly understood. Here, we present a detailed
ecological study of epiphytic lichens in the equatorial montane rain forest of Bwindi National Park (331 km2), Uganda. We evaluated all
major lichen growth forms, including selected groups of crustose lichens. In 14 transects at elevations of 1290 m to 2500 m, we sampled
276 trees belonging to 60 species. We recorded all lichen species on each tree trunk between ground level and 2 m above the ground,
yielding 191 lichen species in 67 genera, with a mean of 4.7 species per tree. We used non-metric multi-dimensional scaling to separate
epiphytic lichen assemblages according to tree species composition and elevation. Structural equation modeling indicated that elevation
influenced tree species composition and that tree species composition largely determined lichen species composition. Thus, elevation acted
indirectly on the lichen assemblages. Further studies examining factors such as bark properties and lichen colonization ecology may clarify
what determines the association between tree species and lichen assemblages. The link between lichen assemblages and large-scale
elevation patterns, as well as disturbance and regrowth histories, warrants further study. An analysis of lichen species composition on
individual tree species that occur over large elevation ranges would distinguish the effect of tree species on lichen assemblages from the
effect of elevation and thus climate. Our study highlights the limited extent of our knowledge of tropical epiphytic lichens.

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(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Uganda, /modeling,
/lichen, /forest

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2015/08/11

Armillaria ectypa, a rare fungus of mire in Poland

Armillaria ectypa

Bibliographic information
M Stasiska - Acta Mycologica, 2015 - pbsociety.org.pl

URL
https://pbsociety.org.pl/journals/index.php/am/article/view/am.1064

Abstract/Summary
Abstract Armillaria ectypa is a saprotroph that occurs on active raised bogs and alkaline fens, as well as Aapa mires and transitional bogs. It
is a very rare and threatened Eurasian species and one of the 33 fungal species proposed for inclusion into the Bern Convention. Its
distribution in Poland, ecological notes and morphology of basidiocarp based on Polish specimens are presented.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Bern, /Poland

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20150811-33

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2015/08/11

Micromycetes on climbing roses leaves (Rosa L.) in the Botanic Garden of the
Jagiellonian University in Cracow

Bibliographic information
M Kowalik, K Duda-Franiak - Acta Mycologica, 2015 - pbsociety.org.pl

URL
https://pbsociety.org.pl/journals/index.php/am/article/view/am.1054

Abstract/Summary
Abstract Micromycetes inhabiting the leaves of 20 cultivars of climbing roses (Rosa L.), grown in Botanic Garden of the Jagiellonian
University in Cracow was investigated in the three successive years of research. Sixty-five taxa of of micromycetes was recorded with a few
species dominating: Alternaria alternata, Epicoccum nigrum, Pestalotia rosae, Penicillium brevicompactum and Sordaria fimicola,
accompanied by various other microfungi. A high abundance of rose black spot caused by Diplocarpon rosae was also observed. The
affected leaves revealed advancing necrosis, substantially enhancing at the end of the growing season. Defoliation took place from June to
October. Micromycetes inhabiting the leaves of climbing roses in Botanic Garden of the Jagiellonian University in Cracow considerably
deteriorated the decorative aspect of the plants.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Rosa, /black spot

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2015/08/11

Micromycetes on ericaceous plant leaves

Bibliographic information
M Kowalik, J Bonio, K Duda-Franiak - Acta Mycologica, 2015 - pbsociety.org.pl

URL
https://pbsociety.org.pl/journals/index.php/am/article/view/am.1055

Abstract/Summary
Abstract A two-year study was carried out on the ericaceous plant collection of the Botanic Garden of the Jagiellonian University in Cracow
and the Rogw Arboretum of the Warsaw University of Life Sciences on the following plants: wild rosemary Ledum palustre L., leatherleaf
Chamaedaphne calyculata (L.) Moench and American cranberry Oxycoccus macrocarpus (Ait.) Pursh. Diverse micromycetes species
composition was specified on the leaves of tested ericaceous plants. The perpetrators of dying leaves were recognized, among which the
dominant role was played by the necrotroph Pestalotiopsis sydowiana. Quantitative and qualitative comparative analyses of micromycetes in
both locations were carried out, showing a comparable degree of plant colonization by these fungi in the botanic garden and arboretum. The
study may be helpful in explaining the causes of dieback of protected and endangered plants in Polish flora.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/garden

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20150811-35

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2015/08/11

Fungal root endophytes of Quercus robur subjected to flooding

Quercus robur

Bibliographic information
H Kwana, W Szewczyk - Forest , 2015 - Wiley Online Library

URL
http://onlinelibrary.wiley.com/doi/10.1111/efp.12212/full

Abstract/Summary
Summary In August 2011, fungal endophytes were isolated from fine (0.10.5 cm diameter) and structural (0.62.0 cm) roots from
eighteen Q. robur trees at six sites in a Q. robur-dominated forest displaying symptoms of oak decline. Five sites were subjected to periodic
flooding for 3 months in 1997 and 1 month in 2010. Fungal colonization was detected in 94% of 1296 root fragments examined. Fungi
representing 126 taxa from Zygomycota (four species), Ascomycota (117 species) and Basidiomycota (five species) were isolated using
culture-based methods and identified by morphotyping and sequencing of the ITS 1 and 2 rDNA. The most frequent eudominants (with
colonization frequencies 10%) were Aspergillus spp., Cosmospora sp., Ilyonectria radicicola, Pezicula radicicola, Pyrenochaeta cava and six
species of Trichoderma. Species composition did not vary greatly between sites, but only 3% of species occurred in all sites. Number of
species was higher in roots subjected to floods. Eudominants and dominants present only on flooded sites included Aspergillus spp.,
Chaetomium globosum, Cosmospora sp., I. radicicola, Sporothrix inflata, Trichoderma harzianum, T. pubescens and T. virens. Structural
roots were usually colonized by a higher number of species than fine roots. The majority of species detected belong to Class 2 NCendophytes. Pezicula radicicola and Phialocephala fortinii belong to Class 4 NC-endophytes.

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(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Quercus, /forest,
/sequencing

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2015/08/11

Phylogeny, identification and pathogenicity of Lasiodiplodia associated with


postharvest stem-end rot of coconut in Brazil

Lasiodiplodia

Bibliographic information
AWC Rosado, AR Machado, FO Friere - Plant , 2015 - Am Phytopath Society

URL
http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-03-15-0242-RE

Abstract/Summary
Coconut palm (Cocos nucifera L.) is one of the most important perennial tropical crops. Stem-end rot is the major postharvest disease of
coconut in Brazil. The fungus Lasiodiplodia theobromae is the only species that has been reported to be associated with this disease.
However, a comprehensive study elucidating the true identity of this pathogen with molecular tools has never been conducted. In recent
years, new species of Lasiodiplodia have been proposed after molecular studies were performed, indicating the existence of a species
complex. The aims of this research were to study the etiology of the postharvest stem-end rot of immature coconut based on a combination
of morphological and phylogenetic analyses, to establish the phylogenetic position of such taxa, and to assess the pathogenicity of each
taxon. Four species were identified: Lasiodiplodia brasiliense, L. egyptiacae, L. pseudotheobromae, and L. theobromae. All of the species
were distinguished morphologically and phylogenetically and were proven to be pathogenic to coconut following artificial inoculation.
Lasiodiplodia theobromae was the most common and the most aggressive species. This study represents the first report of three additional
species of Lasiodiplodia as causal agents of postharvest stem-end rot of immature coconut in Brazil.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Cocos, /Brazil,
/new species, /species complex

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2015/08/11

Selection of Orthologous Genes for Construction of a Highly Resolved Phylogenetic


Tree and Clarification of the Phylogeny of Trichosporonales Species

Bibliographic information
M Takashima, R Manabe, W Iwasaki, A Ohyama - PloS one, 2015 - dx.plos.org

URL
http://dx.plos.org/10.1371/journal.pone.0131217

Abstract/Summary
Abstract The order Trichosporonales (Tremellomycotina, Basidiomycota) includes various species that have clinical, agricultural and
biotechnological value. Thus, understanding why and how evolutionary diversification occurred within this order is extremely important. This
study clarified the phylogenetic relationships among Tricosporonales species. To select genes suitable for phylogenetic analysis, we
determined the draft genomes of 17 Trichosporonales species and extracted 30 protein-coding DNA sequences (CDSs) from genomic data.
The CDS regions of Trichosporon asahii and T. faecale were identified by referring to mRNA sequence data since the intron positions of the
respective genes differed from those of Cryptococcus neoformans (outgroup) and are not conserved within this order. A multiple alignment
of the respective gene was first constructed using the CDSs of T. asahii, T. faecale and C. neoformans, and those of other species were
added and aligned based on codons. The phylogenetic trees were constructed based on each gene and a concatenated alignment.
Resolution of the maximum-likelihood trees estimated from the concatenated dataset based on both nucleotide (72,531) and amino acid
(24,173) sequences were greater than in previous reports. In addition, we found that several genes, such as phosphatidylinositol 3-kinase
TOR1 and glutamate synthase (NADH), had good resolution in this group (even when used alone). Our study proposes a set of genes
suitable for constructing a phylogenetic tree with high resolution to examine evolutionary diversification in Trichosporonales. These can also
be used for epidemiological and biogeographical studies, and may also serve as the basis for a comprehensive reclassification of pleomorphic
fungi.

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(extracted from title and abstract/summary)

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2015/08/11

Tritirachium candoliense sp. nov., a novel basidiomycetous fungus isolated from the
anoxic zone of the Arabian Sea

Tritirachium candoliense

Bibliographic information
T Boekhout, WH Muller, T Stoeck, CS Manohar - 2015 - ndl.iitkgp.ac.in

URL
http://ndl.iitkgp.ac.in:8080/xmlui/handle/12345/52780

Abstract/Summary
A fungal culture (FCAS11) was isolated from coastal sediments of the Arabian Sea during the anoxic season. Multigene phylogenetic
analyses confidentially place the organism as a novel species within the recently defined class Tritirachiomycetes, subphylum
Pucciniomycotina, phylum Basidiomycota. We named the new species Tritirachium candoliense and provide the first description of a
member of this class from a marine environment. DNA sequences and morphological characters distinguish T. candoliense from previously
described Tritirachium species. Its growth characteristics, morphology, and ultrastructural features showed that under anoxic conditions the
species grows slowly and produces mainly hyphae with only few blastoconidia. Electron microscopy revealed differences when the culture
was exposed to anoxic stress. Notable ultrastructural changes occur for example in mitochondrial cristae, irregularly shaped fat globules and
the presence of intracellular membrane invaginations. We assume that the growth characteristics and substrate utilization patterns are an
adaptation to its source location, the seasonally anoxic environment of the Arabian Sea.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/new species

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2015/08/11

Noisy neighbourhoods: quorum sensing in fungal polymicrobial infections

Bibliographic information
EF Dixon, RA Hall - Cellular Microbiology, 2015 - Wiley Online Library

URL
http://onlinelibrary.wiley.com/doi/10.1111/cmi.12490/abstract

Abstract/Summary
Abstract Quorum sensing was once considered a way in which a species was able to sense its cell density and regulate gene expression
accordingly. However, it is now becoming apparent that multiple microbes can sense particular quorum sensing molecules, enabling them to
sense and respond to other microbes in their neighbourhood. Such interactions are significant within the context of polymicrobial disease, in
which the competition or cooperation of microbes can alter disease progression. Fungi comprise a small but important component of the
human microbiome and are in constant contact with bacteria and viruses. The discovery of quorum sensing pathways in fungi has led to the
characterisation of a number of interkingdom quorum sensing interactions. Here, we review the recent developments in quorum sensing in
medically important fungi, and the implications these interactions have on the host's innate immune response.

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(extracted from title and abstract/summary)

Keywords(auto-indexed)
/gene expression

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20150811-44

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2015/08/11

Phylogenetic diversity of culturable endophytic fungi in Dongxiang wild rice (Oryza


rufipogon Griff), detection of polyketide synthase gene and their antagonistic activitiy
analysis

Oryza rufipogon

Bibliographic information
Y Wang, BL Gao, XX Li, ZB Zhang, RM Yan, HL Yang - Fungal Biology, 2015 - Elsevier

URL
http://www.sciencedirect.com/science/article/pii/S1878614615001373

Abstract/Summary
Abstract The biodiversity of plant endophytic fungi is enormous, numerous competent endophytic fungi are capable of providing different
forms of fitness benefits to host plants and also could produce a wide array of bioactive natural products, which make them a largely
unexplored source of novel compounds with potential bioactivity. Although endophytic fungi and bacteria associated with rice (Oryza sativa
L.) have been investigated, little is known about the endophytic fungi of wild rice (Oryza rufipogon). Specifically, no research has focused on
the endophytic fungi diversity of Dongxiang wild rice (O. rufipogon Griff.) in China. This study is the first to reveal the diversity of culturable
endophytic fungi in O. rufipogon G. from China using rDNA-ITS phylogenetic analysis. Here, the potential of fungi in producing bioactive
natural products was estimated based on the beta-ketosynthase detected in the polyketide synthase (PKS) gene cluster and on the bioassay
of antagonistic activity against rice phytopathogens. A total of 229 endophytic fungal strains were validated in 19 genera (Aspergillus,
Alternaria, Bionectria, Bipolaris, Cladosporium, Chaetomium, Dendryphiella, Fusarium, Gaeumannomyces, Leptosphaerulina,
Paraphaeosphaeria, Penicillium, Phoma, Monographella, Sarocladium, Trichoderma, unidentified Dothideomycetes sp., unidentified
Pleosporales sp.1 and unidentified Pleosporales sp.2) in seven orders (Capnodiales, Eurotiales, Hypocreales, Magnaporthales, Pleosporales,
Sordariales and Xylariales) of Ascomycota. Unidentified Pleosporales spp., Phoma spp., Cladosporium spp. and Penicillium spp. were the
dominant players in the culturable endophytic fungal community. The colonisation frequency, isolation rate, Menhinicks index and Shannon
diversity index revealed that seeds were immensely rich in fungal species. The Sorensen similarity index showed the highest tissue-specific

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Oryza, /bioassay,
/China

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2015/08/11

Recent literature on lichens-237

237

Bibliographic information
SZ Hodkinson, BP Hodkinson - The Bryologist, 2015 - BioOne

URL
http://www.bioone.org/doi/abs/10.1639/0007-2745-118.2.231

Abstract/Summary

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(extracted from title and abstract/summary)

Keywords(auto-indexed)

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2015/08/11

A foliar endophyte increases the diversity of phosphorus-solubilizing rhizospheric


fungi and mycorrhizal colonization in the wild grass Bromus auleticus

Bromus auleticus

Bibliographic information
AM Arrieta, LJ Iannone, JM Scervino, MV Vignale - Fungal Ecology, 2015 - Elsevier

URL
http://www.sciencedirect.com/science/article/pii/S1754504815000859

Abstract/Summary
Abstract Asexual Epichlo endophytes establish mutualistic symbioses with grasses, improve fitness of their hosts and modify the
surrounding environment. To test the hypothesis that this symbiotic association increases the abundance and diversity of phosphatesolubilizing fungi (PSF), a pot experiment was conducted combining two endophytic statuses: Epichlo-infected (E+) and non-infected (E)
Bromus auleticus plants, and two soil types collected from agricultural (A) and non-agricultural (NA) fields. Soil fungi were isolated at the
beginning of the experiment and 12 months after the introduction of B. auleticus, and tested for their inorganic P (Pi)-solubilizing capability.
Arbuscular mycorrhizal colonization in B. auleticus roots of E+ and E plants was also analyzed. PSF abundance was affected by the
endophytic status and by the type of soil; the highest value was detected in the ENA treatment, followed by the E+A treatment. PSF
diversity was higher in NA than in A soils and higher in soils treated with E+ than in those treated with E. Arbuscular mycorrhizal fungi
colonization was higher in E+ plants. We hypothesize that the positive association between Epichlo endophytes and mycorrhizal fungi with
an increase in the PSF diversity would generate an increase in the phosphorus (P) available to plants.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/endophyte,
/Bromus, /mycorrhizal fungi

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20150811-51

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2015/08/11

Colonial and Morphological Characteristics of Some Microfungal Species Isolated from


Agricultural Soils in Eskiflehir Province (Turkey)

Bibliographic information
A ASAN, C BAYU, E KINACI - researchgate.net

URL
http://www.researchgate.
net/profile/Engin_Kinaci/publication/272576893_Colonial_and_Morphological_Characteristics_of_Some_Microfungal_Species_Isolated_from_Agri
cultural_Soils_in_Eskiflehir_Province_(Turkey)/links/54e9e6a80cf25ba91c80737d.pdf

Abstract/Summary
Aspergillus crustosusRaper & Fennell, Eupenicillium egyptiacum(J.F.H.Beyma) Stolk & D.B.Scott, Paecilomyces ramosusSamson & H.C.
Evans, and Penicillium novae-zeelandiaeJ.F.H.Beyma were examined for their colonial and morphological propertiesvia visual, light and
scanning electron microscopy. These species isolated from soil in different regions of Eskiehir are recorded forthe first time in Turkey.

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(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Turkey

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20150811-54

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2015/08/11

Zwei aus Finnland beschriebene Risspilze in Deutschland nachgewiesen: Inocybe


urceolicystis Stangl & Vauras und Inocybe ericetorum Vauras & Kokkonen

2Inocybe urceolicystisInocybe ericetorum

Bibliographic information
B Oertel, D Bandini - Zeitschrift fr Mykologie, 2014 - researchgate.net

URL
http://www.researchgate.
net/profile/Ditte_Bandini/publication/271130924_Zwei_aus_Finnland_beschriebene_Risspilze_in_Deutschland_nachgewiesen_Inocybe_urceolicys
tis_Stangl__Vauras_und_Inocybe_ericetorum_Vauras__Kokkonen/links/553b55830cf2c415bb090e4d.pdf

Abstract/Summary
Summary: So far Inocybe urceolicystis Stangl & Vauras and Inocybe ericetorum Vauras & Kok-konen have been known only from
Fennoscandia. In 2012 they were found also in Germany. Further, Inocybe urceolicystis is reported from Estonia. The descriptions of the
two species are complemented by macro- and microphotographs as well by line drawings. In the collections of Inocybe urceolicystis at least
one third of the cheilocystidia have a rounded bottom so that the consistency of this eponymous character could be confirmed. For the
Fennoscandian and Estonian findings of both species a distribution map is shown. The determination of the Ger-man findings of both species
could be assured by compliance with the ITS sequences of the respective holotypes. For the treatment of the genus Inocybe the LSU region
is used in addition to the DNA barcoding region ITS. Methods for alignment and analysis are described that facilitate the interpretation of the
molecular data with Inocybe species. In ITS-LSU cladograms Inocybe urceolicystis occupies an isolated position, while Inocybe ericetorum
belongs to the clade of Napipedinae.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
DNA/DNA barcoding,
/Estonia, /Germany

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2015/08/11

DNA barcodes for marine fungal identification and discovery

DNA

Bibliographic information
S Manokaran, S Velmurugan, T AjithKumar - 2015 - ndl.iitkgp.ac.in

URL
http://ndl.iitkgp.ac.in:8080/xmlui/handle/12345/52770

Abstract/Summary
We employed DNA barcodes for identification of fungal species in marine sediments. Sediments were collected seasonally along the
Southeast coast of India from which a culturable fungal library was constructed. All cultured species were morphologically documented using
microscopical analysis. A maximum population density of 19.3 103 CFU/g was recorded in monsoon and minimum of 3 103 CFU/g in
premonsoon season. Two-way analysis of variance suggests that the fungal community varied significantly between the seasons (F = 9.543,
P < 0.001) and at various depths sampled (F = 4.655, P < 0.05). In total, 54 fungal species belonging to 13 different families were
documented and all species were sequenced for internal transcribed spacer genes. Each species was represented by at least two specimens
constituting a total of 171 specimens for DNA barcoding. Twelve species of a marine fungi were sequenced for the first time. Branching
patterns of phylogenetic tree strongly supported the sequence variations within and between all species barcoded. Based on the pairwise
distance model we suggest barcode gaps of 15 %, 21 %, 30 %, 35 % and 51 % for genera, family, order, class and phyla respectively.

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Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
DNA/DNA barcoding,
/India, /marine fungi

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- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Rapid, specific and sensitive molecular detection assay for Alternaria helianthi that
causes leaf blight disease in sunflower

Alternaria helianthi

Bibliographic information
RL Chavhan, VR Hinge, MB Chinchole - European Journal of , 2015 - Springer

URL
http://link.springer.com/article/10.1007/s10658-015-0716-6

Abstract/Summary
The leaf blight disease of sunflower (Helianthus annuus) caused by Alternaria helianthi is a serious threat to its cultivation worldwide. Early
and accurate detection of the pathogen is critical to efficient disease management and in avoiding further losses due to epidemics in
sunflower. Conventional methods of detection and identification are time consuming, labour intensive, and lack specificity and sensitivity. A
real-time PCR based TaqMan probe assay was developed to target 156 bp Internal Transcribed Spacer (ITS) region of A. helianthi for its
detection using fungal genomic DNA and infected plant tissues and seeds of sunflower. The specificity of the probe and primers was
confirmed by testing their cross reactivity using genomic DNA of closely related Alternaria species isolated from 17 crop plants and 15 fungal
species of other genera. No cross-reactivity could be detected with any of the other non-target fungal strains used in this study. The assay
successfully detected as low as 1.0 pg fungal genomic DNA and up to 1 % infection in sunflower seed lots. To the best of our knowledge,
this is the only probe based real-time PCR assay that enable high specificity and sensitivity for rapid detection of A. helianthi in infected
seeds and plant tissues. The assay may also hold promise for application in effective bio-threat and risk mitigation program by early and
accurate detection of the pathogen for effective management.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Helianthus, PCR/realtime PCR

Daikinrin Daily Document Alert

D Alert #
20150811-59

17

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Food and Water Borne Mycotoxigenic and Mycotic Fungi

Bibliographic information
RRM Paterson, N Lima - researchgate.net

URL
http://www.researchgate.net/profile/Vit_Hubka/publication/279718252_Chaetomium/links/559872b408ae793d137e199d.pdf

Abstract/Summary

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)

Daikinrin Daily Document Alert

D Alert #
20150811-60

17

- the latest mycological papers from Google Scholar


Created byAtsushi Nakajima (@Ats_Nakajima) CC BY 4.0

Search Date

2015/08/11

Temperature affects aggressiveness and fungicide sensitivity of four Pythium spp.


that cause soybean and corn damping off in Iowa

Bibliographic information
R Matthiesen, A Ahmad, A Robertson - Plant Disease, 2015 - Am Phytopath Society

URL
http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-04-15-0487-RE

Abstract/Summary
Damping off of soybean and corn, caused by Pythium spp. is favored by cool temperatures and wet soil conditions and is primarily managed
using fungicide seed treatments. The goal of this research was to determine the effect of temperature on pathogen aggressiveness and
fungicide sensitivity of Pythium spp. recovered from soybean and corn in Iowa. A total of 21 isolates of four of the most prevalent Pythium
spp. in Iowa were screened. Seed and seedling assays were used to quantify the aggressiveness of P. lutarium, P. oopapillum, P.
sylvaticum, and P. torulosum on soybean and corn at 13C, 18C, and 23C. Isolates recovered from soybean or corn were equally
pathogenic on both hosts. P. torulosum was more aggressive at 13C compared to 18C and 23C. Conversely, P. sylvaticum was more
aggressive at 18C and 23C than at 13C. A plate assay was used to assess fungicide sensitivity to seven fungicides that are commonly
used as seed treatments, and EC50 values at each of the three temperatures were determined and compared. EC50 values for P. torulosum
were higher for all fungicides tested at 13C, compared to 18C or 23C, whereas EC50 values for P. sylvaticum were higher for all
fungicides at 18C and 23C compared to 13C. These data contribute to our understanding of the effect of soil temperature on the risk of
damping off in soybean and corn seedlings, which may aid in the development of more effective management practices.

Comment (brief comment in Japanese)

Related taxa
(extracted from title and abstract/summary)

Keywords(auto-indexed)
/Iowa, /soil temperature,
/temperature

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