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Chi-squared (2) test: How to decide if the data fits any of the Mendelian ratios?
Predicted
ratio
Round, yellow
seeds
Round, green
seeds
Wrinkled, yellow
seeds
Wrinkled, green
seeds
Total seeds
Observe
d
(O)
315
108
101
32
556
Expected
(E)
(O-E)2
E
(9/16)(556) =
312.75
(3/16)(556) =
104.25
(3/16)(556) =
104.25
(1/16)(556) =
34.75
556
0.016
0.135
0.101
0.218
0.470
= 0.470
Chi-square Table
freedom (df) = n-1,
Degrees4of Degrees of
Probability
freedom where
0.95 0.90
0.5 no
0.1of classes.
0.05
n is the
1
0.01 0.02 0.46 2.71 3.84
2
0.10 0.21 1.39 4.61 5.99
of classes
= 4, 7.82
so df = 3
3 5 No
0.35
0.58 2.37(n)6.25
4
0.71
1.06 = 4-1
3.36 =7.78
(df
= n-1
3); 9.49
5
1.15 1.61 4.35 9.24 11.07
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Predicted
ratio
Observed
(O)
Expected (E)
Total seeds
(O-E)2
E
X2 =
Degree of freedom =
Conclusion:
2 In pea plants, the allele for smooth seed, W, is dominant over the
allele for
wrinkled seed, w, while the allele for yellow seed, G, is dominant
over the
allele for green seed, g. In one of Mendels dihybrid crosses, pea
plants of
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Degree of
freedom
1
2
3
4
5
6
7
8
9
10
5%
level
3.841
5.991
7.815
9.488
11.070
12.592
14.067
15.507
16.919
18.307
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your answer.
[2]
Genotypes :
...............................................................................................................
................................
Explanation: ..........................................................................................
.....................................................
(b) (i) Explain why a statistical test should be applied to the data
obtained in this investigation.
[2]
...............................................................................................................
...............................................................................................................
...............................................................................................................
...............................................................................................................
...............................................................................................................
..............................................................................................................
(ii) The chi-squared (2) test was applied to the data obtained.
The formula is given below.
Degrees of freedom
1
2
3
4
0.99
0.0002
0.020
0.115
0.297
9.95
0.0039
0.103
0.352
-40.711
Probability value
0.1
0.05
2.71
3.84
4.61
5.99
6.25
7.81
7.78
9.49
0.01
6.63
9.21
11.34
13.28
0.001
10.83
13.82
16.27
18.47 TEA
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(iii)The null hypothesis in this investigation predicted that there would be no difference
between the
observed and expected values. Use the table to determine whether this hypothesis can be
supported.
Explain how you arrived at your answer.
[2]
4 When a heterozygous black rat is crossed with another heterozygous black rat, 43 black, 15
creams, 22 albino
offspring are produced. Using Chi-square test the genetic hypothesis 9:3:4 is consistent with the
data.
- Null hypothesis - the genetic hypothesis 9:3:4 is consistent with the data.
- Level of significance 5%
- Degrees of freedom = n-1 = 3-1 = 2
- Determining expected frequencies ( E )
Black- 9 Total offspring- 80 Black = E = 80 X 9 / 16 = 45
Cream = 80 X 3 / 16 = 15
Albino = 80 X 4 / 16 = 20
Calculated value = 0.28 Table value = 5.96
5 When two heterozygous pea plants are crossed, 1600 plants are produced in the F 2 generation out
of which
940 are yellow round, 260 are yellow wrinkled, 340 are green round and 60 are green wrinkled. By
means of
chi- square test whether these values are deviated from Mendels dihybrid ratio of 9:3:3:1 or by
means of
chi-square test prove whether it is a real independent assortment.
- Null hypothsis There is real independent assortment .
Levels of significance 5%.
- Degrees of freedom n-1 = 4-1=3
- Determining expected frequencies (E) Mendels dihybrid ratio = 9:3:3;1
Yellow round = 9 . Total = 1600 Expected Yellow round = (E) = 1600 X 9 / 16 = 900.
Yellow wrinkled = 3 Expected Yellow wrinkled = 600 X 3 / 16 = 300
Green round = 3 Expected Green round = 1600 X 3 / 16 = 300
Green wrinkled = 1 Expected Green wrinkled = 16600 X 1 / 16 = 100 5 )
Calculated value = 27.43.
Table value = 7.83 Inference :
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Snapdragons are a type of garden plant. A pure breeding strain of a white flowering variety was obtained
and crossed with a pure breeding red flowered strain. The two strains were crossed producing F1 plants
all with pink flowers. The F1 plants were then interbred to produce F2 plants with the following flower
colours:
red
62
pink
131
white
67
The following hypothesis was proposed:
Flower colour in snapdragons is controlled by a single gene with two codominant alleles.
(a)
Complete the genetic diagram to explain this cross. Use the following symbols to represent the
alleles:
R = red flowers
W = white flowers
(b) A chi-squared test is carried out on the data to determine whether the hypothesis is supported or
rejected.
The statistic is calculated in the following way:
(i) Complete the following table.
[2m]
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2
(ii) Calculate the X value for the above data. Show your working.
[1m]
(c) A suitable Null hypothesis would be that there is no significant difference between the observed and
expected numbers.
Biologists consider that if the probability is greater than 5% the deviation is statistically non significant.
(i) Using the figures from the table for 2 degrees of freedom, explain whether you would accept or reject
the Null hypothesis.
[2m]
(ii) What does this suggest about the inheritance of flower colour in snapdragons?
[1m]
GCE A-June 2010
Answers
2 a) Refer to above example
b)
Phenotype
Expected
ratio
Smooth
Wrinkled
Total
3
1
Observed
number
(o)
423
133
556
Expected
number
(e)
417.000
139.000
Divergence
(o-e)
Divergence2
(o-e)2
6.000
-6.000
36.000
36.000
(o-e)2
e
0.086
0.259
= 0.345
The calculated X2 value (0.345) is found to be lower than the value (3.841) given in the X2 table (P0.05,
degree freedom, df = l). Therefore the deviation is not significant and the result fits the 3:1 ratio.
c)
Phenotype
Yellow
Green
Total
Expected
ratio
3
1
Observed
number
(o)
416
140
556
Expected
number
(e)
417.000
139.000
Divergence
(o-e)
Divergence2
(o-e)2
-1.000
1.000
1.000
1.000
(o-e)2
e
0.002
0.007
= 0.009
The calculated X2 value (0.009) is found to be lower than the value (3.84 1) given in the X2 table (P0.05,
degree freedom, df =1). Therefore the deviation is not significant and the result fits the 3:1 ratio.
(a)
Gg / suitable equivalent;
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(i)
(ii)
O
O-E
(O-E)2
E
(OE)2
152
48
150]
50]
2
2
4
4
0.027]
0..08] method ignore calc'n errors);
150
50
2 = 0. 107 / 0.11;
(iii)
[Note: Check carry forward of 2 value or critical value for interpretation or converse argument]
6 (a) RR
R
RW
Pink
WW (allow: Cr Cw/key) 1
W
RW
RR
RW
Red
1
Pink
2
RW
WW
White
1
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