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Cerebrospinal Fluid in Central Nervous System Infections
John E. Greenlee
Karen C. Carroll
Infections within the central nervous system (CNS) frequently, though not invariably, produce changes in ventricular or
lumbar cerebrospinal fluid (CSF). The changes produced may provide invaluable information about the nature of the
infectious process and, in many cases, may permit specific identification of the offending organism. Despite the great
diagnostic value of CSF analysis, however, injudicious attempts to obtain CSF (as in the setting of brain abscess) may cause
severe injury or death, and casual handling of the CSF obtained may render the CSF analysis useless.
This chapter is divided into three parts. The first part reviews the anatomy of the CSF spaces, the physiology of CSF
production and reabsorption, and the effect of infection on CSF physiology and composition. The second part discusses
methods of CSF analysis in CNS infections, and the third part summarizes the CSF analysis in specific CNS infections.
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adherent to bone. Below the foramen magnum, the dura and periosteum diverge and are separated by a fat-filled epidural
space. The middle layer of meninges, the arachnoid, is joined to the dura by a specialized layer of fibroblasts, the dural
border cell layer. The cells of this inner dural border are devoid of collagen and have few cellular junctions, providing a
cleavage plane in which infection may develop and rapidly spread. The arachnoid covers the brain and spinal cord loosely and
extends outward along the course of cranial and spinal nerves.
The third layer of meninges, the pia mater, is continuous with the surface of the brain and spinal cord. The pia mater also
follows vessels into brain and spinal cord parenchyma and projects into the ventricles to form the choroid plexuses. The pia
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mater and the ventricular ependyma merge at the foramina of Luschka and Magendie. The CSF is contained in the
subarachnoid space, enclosed between the arachnoid and the pia. The subarachnoid space surrounds the brain and extends
within the spinal canal to the level of the second sacral vertebra. Within the skull, the subarachnoid space widens into
cisterns where pia and arachnoid are more widely separated by irregularities in the contour of the brain. The largest of these,
the cisterna magna, surrounds the brainstem and the cerebellum at the base of the skull and is occasionally used as a source
of CSF for analysis and culture. The subarachnoid space is crossed by trabecular extensions of the arachnoid itself, by cranial
nerves, by a network of small arteries, the rete mirabile, and by numerous bridging veins, which connect the meningeal veins
with the deeper intracranial venous system (2).
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FIG. 2.1. The cerebral ventricles. Inset: Shown are the structure of the fourth ventricle and the locations of the
foramina of Luschka and Magendie. (From Greenlee JE. Anatomical considerations in central nervous system infections.
In: Mandell GL, Bennett JE, Dolin R, eds. Principles and practice of infectious diseases, 4th ed. New York: Churchill
Livingstone, 1994:821-831, with permission.)
The subarachnoid space is normally a closed system. Occasionally, however, congenital or posttraumatic communications may
exist between the subarachnoid space and superficial tissues and may provide a route for single or recurrent episodes of
meningitis. Congenital defects arise from incomplete closure of the neural tube. These defects may extend for variable
distances into subcutaneous tissues or to the cutaneous surface and are most common in the upper cervical regions and over
the sacrum. Their presence may be suggested by a cutaneous dimple or a patch of hair. Traumatic communications into the
subarachnoid space are most often associated with basilar skull fractures. The most common sites of involvement are (a) the
thin layers of bone that separate the cranial cavity from the paranasal sinuses and (b) the petrous bone, which separates the
auditory canals and mastoid from the cranial cavity. In rare instances, traumatic defects may occur over the cranial
convexities or along the spinal column.
secretion of CSF involves Na ,K -adenosine triphosphatase (ATPase)-mediated transport of sodium across choroidal epithelium
into the ventricular lumen, with water, chloride, and bicarbonate ions following through facilitated transport. The carbonic
anhydrase inhibitor acetazolamide reduces CSF secretion by approximately 50%, whereas furosemide and ethacrynic acid, in
experimental animals, reduce CSF production by 25% to 35% (5). Simultaneous use of both agents reduces CSF formation by
75%.
Reabsorption of CSF occurs through arachnoid villi. Most of these are located along the superior sagittal sinus. Smaller
numbers of arachnoid villi are found along other intracranial venous sinuses and around spinal nerve roots (1). During health,
the arachnoid villi along the superior sagittal sinus provide the major site of CSF uptake. The arachnoid villi along other
sinuses and surrounding spinal nerve roots are thought to provide alternative sites of CSF absorption following superior
sagittal sinus thrombosis.
Each arachnoid villus represents an extension of the arachnoid membrane through the dura mater into the lumen of the
venous sinus and functions as a one-way valve, permitting unidirectional flow from CSF into blood. Early work by Welch (6)
demonstrated that the arachnoid villi have a critical in vitro opening pressure of 2 to 5 cm H O; this study also demonstrated
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that particles up to the size of erythrocytes readily pass from CSF into blood, whereas particles larger than 7.5 m are
excluded. Although these early data suggested that the arachnoid villi might provide a direct communication between CSF
and blood, studies using electron microscopy have demonstrated that arachnoid villi and venous sinuses are separated by a
layer of endothelial cells connected by tight junctions, and that movement of CSF and particulate matter across the
arachnoid villi occurs by transport within giant vesicles (7,8) (Fig. 2.2). These giant vesicles, although they provide efficient
transfer of CSF into blood under normal circumstances, can become obstructed by bacteria and inflammatory cells during
meningitis or by red blood cells (RBCs) during subarachnoid hemorrhage (9,10).
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FIG. 2.2. Uptake of CSF by an arachnoid villus. (From Fishman RA. Cerebrospinal fluid in diseases of the nervous
system, 2nd ed. Philadelphia: WB Saunders, 1992, with permission.)
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provide host defense elsewhere in the body. Normally, T cells and B cells are present in very small numbers in CSF and only
rarely in brain; immunoglobulins and complement are largely excluded from both CSF and brain; and opsonic activity of CSF,
even in the presence of meningitis, is far less than that of serum (11,12,13,14). CNS infections, thus, beginand progressin
tissue that is poorly equipped to halt their spread.
Work during the last two decades has demonstrated that the barrier systems that isolate CSF, brain, and spinal cord from
blood are not static systems but, instead, are highly dynamic in their ability to interact with and transport a wide variety of
substances (15). In addition, it is increasingly recognized that the endothelial cells and astrocytes of the BBB and the
blood-CSF barrier are important sources of cytokines (including tumor necrosis factor [TNF] and interleukins), and that
astrocytes, in addition to their abilities to regulate solute entry into brain, have the ability to act as antigen-presenting cells
(16). As discussed in Chapter 22, release of cytokines by
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endothelial cells and astrocytes in response to bacterial endotoxins and other bacterial products is fundamental in the
production of inflammation and injury during CNS infections and provides an extremely important area for early therapy
(9,17,18,19).
enters CSF both by Na ,K -ATPase-mediated transport during secretion of CSF and by passive diffusion. Potassium is secreted
into CSF by active transport mechanisms and is actively removed from CSF into brain by transport mechanisms that are
believed to be located in astrocyte foot processes. Movement of calcium, magnesium, and phosphorus into CSF and brain also
occurs predominantly by active transport, and the concentrations of these substances are relatively independent of their
concentrations in serum. Chloride and bicarbonate, like potassium, are actively secreted into and actively removed from CSF.
Glucose, amino acids, amines, and thyroid hormone enter the brain by carrier-mediated transport mechanisms (1,15). Insulin
and transferrin require receptor-mediated transport. Although lipids complexed to proteins were once thought to be excluded
from the CNS, it is now known that complexed lipids undergo dissociation from their carrier proteins at the blood-brain
interface and may enter the CNS without significant exodus of protein from brain capillaries.
Chloride represents the major anion in CSF. Normal CSF chloride concentration is 15 to 20 mEq/L higher than that in serum.
Early workers observed that CSF chloride concentrations were lowered in tuberculous meningitis; for many years, levels of
CSF chloride were used to diagnose and follow the course of this infection (1). It is now recognized, however, that the
lowered CSF chloride concentration observed in tuberculous meningitis is nothing more than a reflection of lowered serum
chloride values and has no diagnostic or prognostic value.
The acid-base balance of the CSF, like its electrolyte concentration, tends to remain fairly constant despite fluctuations in
systemic acid-base balance. In CSF, as opposed to plasma, however, movement of CO occurs readily by diffusion, whereas
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movement of bicarbonate occurs more slowly by carrier-mediated transport. The discrepancy in the rate of movement of
these two substances may produce delayed (and, at times, paradoxical) responses in CSF pH as compared to systemic pH
during rapid changes in bicarbonate concentration (1). The CSF acid-base balance is also maintained by the choroid plexuses,
which possess transport mechanisms capable of removing weak organic acidsincluding antibiotics such as the penicillins,
cephalosporins, and aminoglycosidesfrom CSF (21,22). Choroid plexus transport of antibiotics and other weak organic acids
can be blocked by probenecid.
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may also result from functional occlusion of arachnoid villi during severe meningitis or by RBCs in the course of subarachnoid
hemorrhage during bland or septic subarachnoid hemorrhage (10). Thrombosis of the superior sagittal sinus may also block
CSF reabsorption and thereby produce communicating hydrocephalus. Occlusion of a large portion of the superior sagittal
sinus usually produces catastrophic, often hemorrhagic, cerebral infarction. Involvement of the anterior third of the sinus,
however, may be clinically silent except for the development of hydrocephalus.
Obstructive hydrocephalus results from interruption of CSF flow within the ventricular system or at its point of exit into the
subarachnoid space (2). This may be the consequence of infection of the ventricular ependyma or basilar meninges or may
result from extrinsic compression of the ventricular system by infection within brain parenchyma. Lesions producing
obstructive hydrocephalus most commonly involve the ventricular system at its narrowest points: the foramina of Luschka and
Magendie, the fourth ventricle, the aqueduct of Sylvius, and the foramina of Monro. Obstruction of the foramina of Luschka
and Magendie is characteristic of exudative basilar meningitides such as those caused by Mycobacterium tuberculosis,
Coccidioides immitis, and Cryptococcus neoformans but may also be seen in bacterial meningitis. Hydrocephalus as a result of
obliteration of the fourth ventricle is almost always extrinsic and is the result of ventricular compression by large cerebellar
mass lesions such as cerebellar abscess or hemorrhage. Occlusion of the aqueduct of Sylvius by granulomatous ependymitis
may occur as a complication of tuberculosis, fungal infections, or sarcoidosis. Mumps virus, which replicates in ventricular
ependymal cells, has been shown to produce congenital aqueductal stenosis in experimental animals (23). Rare cases of
acquired aqueductal stenosis have also been reported following mumps meningoencephalitis in humans (24). Extrinsic
compression of the aqueduct of Sylvius may be produced by abscesses or other localized infections
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within the pons or midbrain. Involvement of the foramen of Monro is almost always unilateral and is the consequence of
severe brain shifts caused by abscess, focal encephalitis, or hemorrhage. Hydrocephalus caused by the occlusion of one
foramen of Monro is particularly dangerous because the CSF trapped within the involved lateral ventricle acts as a unilateral
space-occupying lesion, greatly increasing the risk of transtentorial brain herniation.
Computerized tomography (CT) and magnetic resonance imaging (MRI) are invaluable in demonstrating the presence of
hydrocephalus and in determining its cause. Ventricular dilationso-called hydrocephalus ex vacuois common in the elderly
and is characterized by symmetric ventricular dilation accompanied by evidence of cerebral cortical atrophy. In contrast,
hydrocephalus from impaired CSF circulation is accompanied by loss of cortical markings visible on CT or MRI as the brain is
forced outward against the skull and by periventricular areas of increased lucency, representing transependymal leakage of
CSF. Communicating hydrocephalus and hydrocephalus from obstruction of the foramina of Luschka and Magendie are
characterized by symmetric enlargement of all four ventricles. Hydrocephalus from occlusion of the fourth ventricle or
aqueduct of Sylvius results in loss of that structure on CT or MRI, with dilation of the third and lateral ventricles.
Hydrocephalus following compression of the foramen of Monro is almost invariably associated with an identifiable spaceoccupying lesion and a prominent midline shift. Thrombosis of the superior sagittal sinus may be difficult to detect as a cause
of communicating hydrocephalus. CT imaging, even with enhancement, does not reliably detect superior sagittal thrombosis.
For this reason, the diagnostic method of choice is MRI (25). The diagnostic value of MRI is based on the finding that protons
carried within flowing blood pass rapidly out of the plane of radiographic section, whereas protons within clotted blood do
not. Thus, blood moving within vessels produces a signal void, whereas clotted blood produces an increased signal on both T1and T2-weighted images. The sensitivity of conventional MRI can be enhanced by use of magnetic resonance venography (25).
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that must be instilled into the subarachnoid or ventricular spaces before an increase in pressure occurs (27). In healthy
individuals, instillation of large volumes of artificial CSF has little effect on ICP. Once intracranial compliance is exhausted,
administration of very small amounts of artificial CSF (1 mL or less) may cause a precipitous increase in ICP. Although
measurement of compliance may provide a more accurate assessment of patient status than measurement of ICP, the
technique is not routinely used.
The elevation in CSF pressure seen in infections and other pathologic conditions is not constant but fluctuates considerably.
This fluctuation is usually not observed during the brief period of measurement provided by lumbar puncture but becomes an
important parameter to observe during monitoring of ICP. Minor variation in pressure occurs during Cheyne-Stokes respiration
and during variations in blood pressure produced by Hering-Breuer reflexes. More major variations in ICP occur during plateau
waves. These are abrupt elevations in ICP (usually lasting 5 to 20 minutes) in which ICP may reach 600 to 1,300 mm of CSF (50
to 100 mm Hg) (28). Plateau waves are believed to represent a consequence of disturbed cerebrovascular autoregulation
because of either abnormal sympathetic tone or cyclic changes in perfusion in which mild hypotension is followed by cerebral
vasodilation and increased cerebral blood flow (28). Although plateau waves may be without any detectable clinical effect,
they may also be associated with signs of brainstem compression and impending herniation.
Increased pressure that exceeds intracranial compliance causes downward and backward shifting of the cerebrum and
brainstem (29). Minimal degrees of shift are well tolerated, but a more extensive shift may cause herniation of the cingulate
gyrus beneath the falx cerebri, herniation of the uncus of the temporal lobe over the tentorium cerebelli, and ultimately,
herniation of the lower brainstem and cerebellar tonsils into the foramen magnum. Herniation of the cingulate gyrus is usually
asymptomatic. Uncal herniation, however, initially produces compression of the third cranial nerve as it passes beneath the
tentorium; it subsequently causes compression of the midbrain, with resultant coma. The aqueduct of Sylvius is often
occluded during uncal herniation, and the resultant hydrocephalus increases the mass effect already present. Herniation of
the cerebellar tonsils through the foramen magnum, with compression of medullary respiratory centers and respiratory arrest,
is often the terminal event in CNS infections. Occasionally, space-occupying lesions within the cerebellum cause upward
herniation of posterior fossa contents through the tentorial notch (30). Extreme elevation of CSF pressure may elevate ICP
above systemic
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arterial perfusion pressure, producing global cerebral and brainstem infarction.
Elevation in CSF pressure, as monitored by ICP monitoring devices, may provide an indication of prognosis in bacterial
meningitis and possibly in other CNS infections. Rebaud et al. (31) found that CSF pressures were significantly higher in
patients who succumbed to meningitis or encephalitis than in patients who survived, and they also found that cerebral
perfusion pressure (mean systemic arterial pressure minus ICP) was significantly lower in the former than in the latter.
Goitein and Tamir (32) found that all patients with meningitis or encephalitis who had a cerebral perfusion pressure more
than 30 mm Hg survived, whereas those with lower pressures died.
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it usually develops within a few hours of the procedure, as CSF continues to leak through the arachnoid at the site of
puncture.
The likelihood of brain herniation following lumbar puncture in the setting of elevated ICP is greatly influenced by the nature
of the underlying pathologic process and its degree of severity. With rare exceptions, there is little risk of herniation
following lumbar puncture in the setting of viral meningitis, and similarly, lumbar puncture can be performed safely in
patients with subarachnoid hemorrhage unless extensive parenchymal hemorrhage is present as well. Lumbar puncture can
also be performed safely in most patients with bacterial meningitis. However, severe bacterial meningitis may be
accompanied by a number of conditions that render brain herniation likely to occur. These include cortical encephalitis,
microabscesses, cerebral edema, hydrocephalus, and venous sinus or cortical vein thrombosis with hemorrhage (34,35,36).
Rennick, Shann, and de Campo (34) identified brain herniation in 19 of 443 children with bacterial meningitis: herniation was
present in 14 of 31 patients who had a fatal course. Lumbar puncture should, thus, be approached with caution in patients
with bacterial meningitis if one suspects severe elevation of ICP and impending herniation. In such patients, a 22- or 25-gauge
needle should be used, the patient should be watched closely for signs of impending herniation during the hours following the
procedure, and the use of dexamethasone, hyperventilation, and mannitol to lower ICP should be strongly considered, along
with placement of a device to monitor ICP. In extreme cases, it may be most prudent to treat the presumed meningitis with
broad-spectrum antibiotics, achieve medical control of intracranial hypertension, and defer lumbar puncture until the patient
is more stable (37). Careful clinical evaluation is usually able to define patients likely to have abnormal CT scans in the
setting of bacterial meningitis (38). However, the likelihood of brain herniation in the setting of meningitis with elevated ICP
may not be accurately predicted by CT or MRI (34).
The presence of space-occupying lesions significantly increases the risk of brain herniation. Brain abscess represents a rapidly
expanding space-occupying lesion in which focal brain displacement is present from the outset, and the risk of herniation
following lumbar puncture is 10% to 20% (33,39). Subdural empyema, which represents an even more rapidly expanding lesion,
presents a similar or greater risk. Herniation may also follow lumbar puncture in obstructive hydrocephalus (in this setting,
trapped intraventricular CSF behaves as a mass lesion), in patients with large intracranial hemorrhages, and in conditions
associated with extensive cerebral edema such as severe herpes simplex encephalitis, rickettsial encephalitis, or Reye
syndrome.
Patients with suspected meningitis who also have focal findings suggesting brain abscess present a significant clinical
dilemma. Lumbar puncture in such patients is of obvious diagnostic use, but the need to rule out localized infection by CT or
MRI may delay lumbar puncture for as long as several hours. Where concern exists that both meningitis and brain abscess may
be present, presumptive antibiotic therapy should be started immediately. A CT or MRI scan should then be obtained
emergently to exclude abscess, and the patient should undergo lumbar puncture after loculated infection or impending
herniation from the meningitis itself has been excluded. A similar concern may arise in the setting of spinal epidural abscess
or rarely spinal subdural empyema. In this setting, as in suspected brain abscess, presumptive antibiotic therapy should be
initiated, and MRI should be obtained on an emergent basis. CT myelography should be employed if MRI is not available.
Spinal fluid should be obtained at the time of myelography or after a compressive lesion has been ruled out by MRI.
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the setting of bacteremia might result in meningitis. This concern is based partly on experimental work by Petersdorf,
Swarner, and Garcia (41), who demonstrated that 81% of dogs undergoing cisternal puncture in the presence of severe
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bacteremia (>10 organisms/mL of blood) developed meningitis, whereas meningitis did not develop in similarly bacteremic
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control animals not undergoing cisternal puncture. Bacteremia at levels higher than 10 organisms/mL of blood, though
unusual in adult infections, may occur in up to 30% of patients with neonatal sepsis, and noteworthy is that investigators have
reported an association between meningitis and lumbar puncture in the setting of bacteremia in children younger than 1 year
but not in older children (42,43). Although these cases are of interest, normal CSF at the time of initial lumbar puncture does
not exclude the possibility that meningitis was already present in its early stages. The diagnostic usefulness of lumbar
puncture in the evaluation of febrile patients with suspected CNS infection far outweighs any small risk that the procedure
itself might lead to meningitis.
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FIG. 2.3. Gadolinium-enhanced MRI scan of a patient with intracranial hypotension. There is diffuse, symmetric
meningeal enhancement (arrows).
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acid-fast stains of CSF sediment, and Gram stain and bacterial culture of the fluid. Differentiation of bacterial meningitis
from viral, mycobacterial, or fungal meningitis on the basis of CSF abnormalities is presumptive unless an organism is seen
and unless the differentiation rests not on only one of these tests but on their sum. Amounts of CSF required by most
laboratories for commonly obtained determinations are listed in Table 2.1. Because clinical laboratories differ in the amounts
of CSF required for individual tests, however, the clinician must determine the amounts of CSF required by the hospital
laboratory for each intended test before performing the lumbar puncture.
CSF Pressure
CSF pressure must be measured in the lateral decubitus positionwith the patient lying horizontally, on his or her side. The
head of the bed should be flat, rather than elevated. Variations in posture and patient size make measurement of ICP
unreliable
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with the patient sitting. Opening CSF pressure in healthy adults, with the patient in the lateral decubitus position, lies
between 50 and 195 mm CSF (3.8 to 15.0 mm Hg) (1). Values less than 150 mm CSF are clearly normal, those between 150 and
200 mm are suspicious, and those higher than 200 mm are abnormal. Normal lumbar CSF pressures in neonates and premature
infants are significantly lower, with mean values of 100 mm H O and 95 mm H O, respectively (53). CSF pressure is not
2
affected during pregnancy (54). However, the CSF pressure can be spuriously elevated by Valsalva maneuver in an anxious or
combative patient and may be falsely lowered by hyperventilation. Delay in obtaining the pressure reading over several
minutes may reduce pressure by allowing fluid to escape around the needle at its point of entry into the subarachnoid space.
Extreme elevation of CSF pressure may herald impending brain herniation. If significantly elevated pressure is found on
lumbar puncture, serious consideration should be given to both (a) use of measures to lower ICP and (b) continuous
monitoring of ICP and arterial pressure. Occasionally, CSF pressure may be normal or even low in the setting of ongoing
tonsillar herniation. The falsely low readings obtained in this setting are believed to reflect occlusion of the CSF space at the
foramen magnum by the herniated tonsils wedged against the lower brainstem. The possibility of complete spinal block should
be kept in mind if CSF pressure falls to zero during the procedure (see earlier discussion).
TABLE 2.1. MINIMAL VOLUMES OF CSF REQUIRED FOR COMMON DIAGNOSTIC TESTS
Test
Volume of CSF
Required
0.5-5.0 mL
0.5 mL
Bacterial culture
3-5 mL
Mycobacterial culture; fungal culture (includes acid-fast smear and India ink
preparation)
20 mL
1-2 mL
Cryptococcal antigen
0.5 mL
VDRL
0.5 mL
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2 mL + serum
>a
Volumes required represent minimal quantities of CSF required by most hospital laboratories. The clinician
should determine the amounts of CSF required by his or her hospital laboratory by each intended test before
performing the lumbar puncture.
Approximately 0.5 mL will be needed for cell count. Amount of CSF required for differential will vary,
depending on whether cytocentrifugation is used or material from centrifuged CSF sediment is studied.
c
Blood drawn before initiating the lumbar puncture should also be submitted with spinal fluid for determination
of simultaneous blood glucose level.
d
As little as 0.5 mL may be submitted for culture if there is great difficulty obtaining fluid. However, the use of
centrifuged sediment from larger volumes of CSF will improve yield on culture in acute bacterial meningitis. The
use of large volumes of CSF is essential in more chronic infections.
e
Yield on culture for acid-fast bacilli and fungi is, in general, extremely poor unless large volumes of CSF (20 mL
or more in adults) are cultured.
f
Serum (2-5 mL) drawn before or after the lumbar puncture should be submitted for electrophoresis along with
CSF.
become turbid as a result of entry of cells, bacteria, or fat; it can be made turbid by as few as 200 WBCs/mm or 400
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RBCs/mm (1,55). CSF containing RBCs will be grossly bloody if 6,000 or more RBCs are present per cubic millimeter, and it
will be cloudy and xanthochromic or pinkish if 400 to 6,000 cells are present (1). In few patients, turbidity may result from
bacteria or fungi in the absence of cells. Rarely, epidural fat aspirated at the time of lumbar puncture can give a turbid
appearance to the CSF (56).
The CSF may be discolored by the presence of breakdown products of RBCs or by protein, bilirubin, or other pigments.
Discoloration of CSF by intact RBCs usually results in a reddish discoloration and turbidity. Although the average life span of
an RBC is 120 days in the circulation, rapid lysis of RBCs occurs in CSF. This results in a yellowish discoloration termed
xanthochromia or xanthochromasia. In most patients, xanthochromia begins to appear approximately 2 to 4 hours after RBCs
have entered the subarachnoid space. In 10% of patients, however, the appearance of xanthochromia may be delayed for 2 to
4 hours and is occasionally not seen for as long as 12 hours. Because CSF may remain colorless during the first 2 to 4 hours
after the onset of subarachnoid hemorrhage, the absence of xanthochromia during this period cannot be used as evidence of a
traumatic puncture. Xanthochromia may also develop within 1 hour in vitro after CSF has been removed, an important
consideration when the CSF obtained at lumbar puncture has been contaminated by a traumatic tap.
Xanthochromia resulting from lysis of RBCs is initially a result of oxyhemoglobin. After 12 hours, the pigment represents
predominantly bilirubin (1). Leakage of methemoglobin from a chronic parenchymal or subdural hemorrhage may also produce
xanthochromia. Xanthochromia may also represent the presence of increased amounts of protein, as described later, or may
be a consequence of systemic hyperbilirubinemia with a bilirubin level higher than 10 to 15 mg/dl. In rare instances,
xanthochromia may be caused by malignant melanoma metastatic to the meninges.
Viscosity of CSF is usually relatively little affected by the presence of meningeal infection or irritation. A qualitatively
appreciable change in CSF viscosity, however, may be seen in severe cryptococcal infections and is believed to represent
capsular polysaccharides (1). Similar viscosity may be produced by widespread metastasis of adenocarcinoma; in such cases,
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TABLE 2.2. NORMAL CSF VALUES OF IMPORTANCE IN INFECTIOUS DISEASES OF THE NERVOUS SYSTEM:
VALUES IN ADULTS, TERM INFANTS, AND PREMATURE INFANTS
Adults
Term
Infants
<5
61
Mean
30
90
115
Range
9-58
20-170
65-150
62
52
50
45-80
34-119
24-63
Mean
0.60
0.81
0.74
Range
0.5-0.8
0.44-2.48
0.55-1.55
Parameter
Premature
Infants
57
Glucose (mg/dl)
Mean
Range
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Cell counts in term and premature infants represent mean values. The range of cell counts found in normal
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neonates is 0-32 cells/mm and in premature infants is 0-29, with 2 standard deviations encompassing a range of
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0-22.4 cells/mm in term and 0-24.4 cells/mm in premature infants. By 1 month of age, normal CSF contains <20
3
cells/mm (2).
b
Rare polymorphonuclear leukocytes may be seen in cytocentrifuged samples of CSF from normal adults. This is
3
not necessarily abnormal if the CSF leukocyte count is 4 cells/mm or less and if protein and glucose levels are
normal.
c
Assumes a blood glucose level of 70-120 mg/dl. At high blood glucose levels (700 mg/dl), normal lower limit of
CSF:blood glucose ratios may approach 0.4 (see text).
Adapted from Fishman RA. Cerebrospinal fluid in diseases of the nervous system, 2nd ed. Philadelphia: WB
Saunders, 1992, with permission.
counters are inaccurate for counts less than 1,000 cells/mm and should not be used to count CSF (1). The accuracy of the
cell count is open to question unless the specimen is examined immediately after the lumbar puncture has been completed.
Normally, CSF contains fewer than five cells per cubic millimeter (Table 2.2). Most of these cells are small lymphocytes
(nuclear diameter about 6 to 7 m) with scant cytoplasm. The presence of PMN leukocytes should be regarded with concern
(57). Occasionally, however, one to two PMN cells per cubic millimeter will be detected in otherwise normal CSF (1,48,57).
Larger numbers of PMN leukocytes are abnormal in uncentrifuged CSF. C. neoformans is similar in size to a small CSF
lymphocyte and may be mistaken for these cells in the counting chamber, though not in stained cytocentrifuged or otherwise
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concentrated samples. Neonatal CSF usually contains 8 to 9 WBCs/mm , and up to 32 WBCs/mm has been reported in the
absence of disease (58) (Table 2.2).
Parasitic infestations
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Angiostrongylus cantonensis
Gnathostoma spinigerum
Trichinella spiralis
Ascaris lumbricoides
Toxoplasma gondii
Toxocara cati
Toxocara canis
Mycobacterium tuberculosis
Treponema pallidum
Mycoplasma pneumoniae
Fungal meningitides
Granulomatous meningitis
Malignant lymphoma
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Hodgkin disease
Leukemia
Multiple sclerosis
Subarachnoid hemorrhage
CSF Glucose
Most glucose present in CSF (Table 2.2) moves across the choroid plexus and across ventricular and subarachnoid capillaries by
facilitated transport. A smaller amount of glucose enters the CSF by simple diffusion. Glucose is removed from CSF through
utilization by cells lining the ventricles and subarachnoid space and by transport across capillaries and arachnoid villi. Entry of
glucose occurs over time, and more than 2 to 4 hours is required before serum and CSF glucose levels reach equilibrium (1). In
the absence of infection or other pathologic conditions, CSF glucose levels are a predictable reflection of blood glucose, and
the ratio of CSF to blood glucose concentrations is approximately 0.6. The CSF glucose level, equilibrated with a normal blood
glucose level of 70 to 120 mg/dl, thus ranges between 45 and 80 mg/dl (Table 2.2). Levels of glucose in ventricular fluid are 6
to 18 mg/dl higher than those in lumbar fluid (1,67).
CNS infections may alter glucose transport across the blood-CSF barrier, resulting in a low CSF glucose level, termed
hypoglycorrhachia (1). Further reduction in CSF glucose levels may result from glucose consumption by WBCs and organisms
(1). Reduction of CSF glucose relative to blood glucose is characteristic of meningitis because of bacteria, mycobacteria, or
fungi (68,69). The CSF glucose level is usually normal during viral infections. However, low CSF glucose levels are occasionally
observed in meningoencephalitis caused by mumps, enteroviruses, lymphocytic choriomeningitis, herpes simplex, and herpes
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zoster viruses (70,71,72,73). Low CSF glucose values have also been described in CNS complications of Mycoplasma
pneumoniae infection, carcinomatous meningitis, CNS sarcoidosis, and subarachnoid hemorrhage (69,74,75,76,77). During
recovery from meningitis, CSF glucose levels tend to return toward normal more rapidly than cell counts and protein levels,
making CSF glucose levels an important parameter to follow in assessing response to therapy (78,79).
Both reduction in CSF glucose values and altered ratios of CSF to blood glucose levels are used as indicators of infection.
However, the literature contains a variety of recommendations about the point at which CSF glucose should be considered
abnormally low (58,71); this is partly because of the prolonged interval over which CSF glucose equilibrates with serum
glucose (1,67,68,80). In general, a CSF/blood glucose ratio less than 0.5 should be considered abnormal. In premature and
full-term infants, however, the normal CSF/blood glucose ratio is 0.74 to 0.96, and a ratio of 0.6 is usually considered
abnormal (58,80). In severe hyperglycemia, transport of glucose into CSF may lag, and at a blood sugar level of 700 mg/dl,
the CSF/blood glucose ratio may approach 0.4. For this reason, a ratio of 0.3 has been suggested as abnormal in diabetics
(81). Silver and Todd (71) addressed the problem of diagnostically significant hypoglycorrhachia in a study of 181 pediatric
patients with CSF glucose levels less than 50 mg/dl or a CSF/blood glucose ratio less than 50%. Patients ranged in age from
younger than 1 week to 14 years, with an average age of 1 years. Their series included patients with bacterial meningitis,
aseptic meningitis, subarachnoid hemorrhage, and CNS carcinomatosis but did not include patients with tuberculous or fungal
meningitis. Blood for glucose analysis was obtained 1 to 114 minutes before the lumbar puncture (average interval, 30
minutes). Of 35 patients with bacterial meningitis in this series, 27 (77%) had CSF glucose levels of 20 mg/dl or less, whereas
CSF glucose levels of 20 mg/dl or less were found in only 10 (7%) of 146 patients with other conditions; and of 37 patients
with glucose levels less than 20 mg/dl, 27 (73%) had bacterial meningitis. A CSF glucose level less than 20 mg/dl or a
CSF/blood glucose ratio less than 0.30 was highly correlated with bacterial meningitis, whereas an absolute CSF glucose value
between 20 and 50 mg/dl was nonspecific; also, a CSF/serum glucose ratio greater than 0.3 was felt to exclude most (but not
all) cases of bacterial meningitis. More recently, Spanos, Harrell, and Durack (82) analyzed the records of 422 patients with
acute bacterial or viral meningitis. These workers found that CSF glucose levels less than 18 mg/dl (1.9 mmol/L) and a
CSF/blood glucose ratio less than 0.23 were individual predictors of bacterial as opposed to viral meningitis, with 99% or
better certainty (82).
CSF Protein
Protein is largely excluded from CSF by the blood-CSF barrier and, under normal conditions, reaches CSF by pinocytotic
transport across capillary endothelia (1). Total CSF protein concentration in lumbar CSF of a healthy adult (Table 2.2) is less
than 40 mg, and the CSF/serum ratio of albumin is 1:200 (1,13). Mean values of lumbar CSF protein in healthy children and
adults have ranged from 23 to 38 mg/dl, and the extreme upper and lower concentrations have been 58 and 9 mg,
respectively (1). The CSF protein level in premature and full-term neonates may range between 20 and 170 mg/dl, with a
mean of 90 mg/dl (58) (Table 2.2). Protein concentrations in cisternal and lumbar CSF are lower, ranging from 13 to 30 mg/dl
(1). Elevation of protein concentration in the setting of CNS infections results from disruption of tight junctions between
endothelial cells of venules and, to a lesser extent, other small meningeal or parenchymal vessels (83). Elevation of CSF
protein level to more than 150 mg/dl may cause the CSF to be xanthochromic. Extreme elevation of protein (to >1.5 g/dl)
may cause formation of a weblike surface pellicle or an actual clot, as may high levels of fibrinogen (1). Levels of CSF protein
may be falsely elevated by deteriorating RBCs following subarachnoid hemorrhage or traumatic lumbar puncture. The amount
of increase is roughly 1 mg/dl per 1,000 RBCs. Accurate assessment of the contribution to total CSF protein made by RBCs
requires that the cell count and protein determination be carried out on the same tube of CSF.
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Changes in the concentration of protein in CSF are the most common and least specific of CSF alterations in disease and are
seen in a wide variety of infectious and noninfectious neurologic conditions. Thus, an elevated CSF protein level, taken alone,
has little specific value in the diagnosis of CNS infections. Elevation of CSF protein to levels more than 100 mg/dl, particularly
if obtained on serial lumbar punctures, argues against viral infection, however, and Spanos, Harrell, and Durack (82) have
recently demonstrated that elevation of protein to a level of 220 mg/dl (2.2 g/L) suggests bacterial as opposed to viral
meningitis, with 99% or greater certainty. The CSF protein levels return to normal more slowly than glucose levels and cell
count during recovery from meningitis and may remain abnormal for months after parenchymal infections. Although elevation
of CSF protein is common in CNS infections, normal protein values are occasionally seen in all types of CNS infections,
including bacterial meningitis.
CSF Immunoglobulins
Immunoglobulins are almost totally excluded from normal CSF. The blood/CSF ratio of immunoglobulin G (IgG) in normal CSF
is usually in the range of 500:1. Immunoglobulin M (IgM) is essentially absent from CSF. Studies with radioiodinated IgG have
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demonstrated that CSF IgG in healthy individuals is derived entirely from serum, requiring 3 to 6 days to reach equilibrium
(84). Immunoglobulins enter CSF less readily than albumin; and in health, immunoglobulin/albumin ratios in CSF are reduced
relative to those in serum. Elevation in CSF immunoglobulins may follow disruption of the BBB, allowing passage of
immunoglobulins across capillary endothelium, or may result from local antibody synthesis within the brain. Increased levels
of CSF IgG per se have little diagnostic value in CNS infections. Detection of oligoclonal IgG bands unique to CSF and not seen
in serum on gel electrophoresis provides strong evidence for an ongoing immune response within the brain. Oligoclonal bands
have been described in a variety of acute and chronic CNS infections of bacterial, mycobacterial, fungal, and viral origins, as
well as in a number of noninfectious neurologic disorders, so detection of oligoclonal bands in CSF is not reliable evidence of
infection.
organisms present. Work by LaScolea and Dryja (85) has shown that 25% of smears will be positive with 10 or fewer colony3
forming units (CFU) of bacteria per milliliter, 60% with 10 to 10 CFU/mL, and 97% with more than 10 CFU/mL. In general,
Gram stain is positive in 60% to 80% of untreated patients (85,86,87). The yield is approximately 20% lower in patients who
have received prior antibiotic therapy (86,87,88). Several pitfalls exist in obtaining an accurate study, all of which are largely
correctable with patience and experience. Haste in carrying out an examination of the material may allow the examiner to
miss organisms present in small numbers. This is less true for gram-positive organisms than for gram-negative bacteria and is
particularly true in the case of Neisseria meningitidis, which tends to be intracellular. Staphylococcus aureus may be
mistaken for streptococci if present as individual organisms. Listeria monocytogenes may be mistaken for diphtheroid
contaminants or for Streptococcus pneumoniae. False-positive Gram stains may result from bacteria present in the collecting
tubes, slides, or reagents, or rarely from bacterial contamination from a skin fragment excised by a spinal needle used
without its stylet (89,90).
Microscopic examination and culture require that the specimen be concentrated. Recommendations are that for volumes
more than 0.5 mL, the specimen be centrifuged for a minimum of 15 minutes at 3,000 g (30 minutes at 1,500 g).
Observation of bacteria on Gram stain preparations can be enhanced using cytocentrifugation. Two studies indicate that slides
prepared in a cytospin centrifuge (Shandon Southern Products Ltd., Cheshire, England) improve microscopic detection of
organisms in Gram-stained CSF when compared to conventional centrifugation (91). Leukocyte morphology is also preserved.
A disadvantage of this technique is the requirement for 0.4 to 0.5 mL of specimen, which is then not available for other
studies. If the volume of CSF available for culture and microscopic studies is less than 0.5 mL, then the entire unspun
specimen should be used for microscopic examination and culture (91).
The sensitivity of the Gram stain procedure varies to some extent with the offending organism. Organisms will be detected by
Gram stain in almost 90% of cases of pneumococcal or staphylococcal meningitis, 86% of cases caused by Haemophilus
influenzae, and 75% of patients with N. meningitidis meningitis (92). In contrast, organisms are present on Gram stain in only
50% of cases of gram-negative meningitis and in fewer than 50% of cases of meningitis caused by L. monocytogenes or
anaerobic organisms.
The utility of additional stains such as acridine orange, which may be used in conjunction with or in place of Gram stain, is
reviewed elsewhere (93). The acridine orange stain is a fluorochrome stain that has been shown to improve detection of
bacteria in CSF specimens, especially in patients who have partially treated bacterial meningitis (93). The Quellung test,
which uses a polyvalent antiserum against capsular antigens of bacteria such as S. pneumoniae, has been supplanted by other
tests for detection of bacterial antigens.
Acid-fast Stain
The sensitivity of the acid-fast stain depends greatly on the skill and persistence of the examiner and the amount of fluid
concentrated. In general, as large a volume of CSF as possibleat least 20 mLshould be taken for smear and culture unless
contraindicated by the presence of elevated ICP or a
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focal mass lesion (69). In most series, acid-fast bacilli (AFB) have been detected in the first sample in fewer than 37% of
patients, although organisms may be detected in up to 87% of patients if material from four different lumbar punctures is
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evaluated by experienced personnel (94). The likelihood of detecting organisms on acid-fast stain will be far less, however, if
only 1 to 2 mL of CSF is used to prepare the smear, if the examiner is unskilled, or if insufficient time is spent examining the
specimen. In a retrospective review of patients with tuberculous meningitis, Barrett-Connor (95) found that the AFB stain was
positive in only 2 of 21 patients. In a similar study of 43 children with tuberculous meningitis, Idriss, Sinno, and Kronfol (96)
found a positive AFB stain in only 5 (12%). In the series by Roberts (97), all 13 samples sent for AFB stain from patients who
were eventually found to have tuberculous meningitis were negative. Sensitivity of the acid-fast stain can be considerably
increased by immunofluorescence methods using auramine-rhodamine (98).
FIG. 2.4. Gram stains of CSF from patients with bacterial meningitis. A: Streptococcus pneumoniae. B: Neisseria
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FIG. 2.5. India ink preparation of CSF, from a patient with cryptococcal meningitis. The capsule of a cryptococcal
organism is clearly outlined by ink particles.
Wet mount preparations may be used to identify motile trophozoites in the CSF of patients with primary amebic
meningoencephalitis (103). Search for motile organisms in wet mounts may be made more reliable by the use of phasecontrast microscopy.
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Choice of culture media, methods of handling, and lengths of time over which cultures are to be maintained are thoroughly
discussed in standard reviews and texts (100,104,105). The CSF should be submitted to the laboratory immediately after the
lumbar puncture and should be placed in culture promptly to avoid loss of fastidious organisms such as H. influenzae, N.
meningitidis, or anaerobes. CSF cultured for bacteria should, at a minimum, be plated on a 5% sheep blood agar, chocolate
agar, and inoculated into an enrichment broth (e.g., thioglycolate, Columbia, Brucella, supplemented peptone, or eugonic)
(104). A minimum of 2 mL (ideally 5 mL or more) should be submitted for Gram stain and bacterial culture. It is important for
the clinician to communicate with the laboratory if the patient has a condition that predisposes to anaerobic meningitis so
appropriate additional media can be added to the routine cultures.
Mycobacterial culture methods for the diagnosis of tuberculous meningitis overall have been disappointing: cultures may take
6 weeks or longer to become positive, and yield on culture in most Western countries is between 52% and 78% (94,106,107).
For this reason, there is great interest in newer molecular techniques employing the polymerase chain reaction (PCR) (see
Polymerase Chain Reaction Methods, later in this chapter). If infection by M. tuberculosis or fungi is suspected, at least 20
mL of CSF should be submitted for culture if not contraindicated by intracranial mass lesions or hydrocephalus, and 40 to 50
mL should be cultured if at all possible. Frequently, a second lumbar puncture is performed to obtain these larger volumes.
Sensitivity of cultures varies widely. The laboratory workers should be informed if cocci or Histoplasma infection is suspected
because of the risks.
C. neoformans is cultured from the CSF in approximately 72% of patients on the first lumbar puncture and in more than 90%
on multiple attempts (69,108). Frequency of recovery of C. albicans from CSF is also high (69,109,110). Isolation of other
organisms such as Histoplasma capsulatum or Brucella species often proves difficult (69,111). In both mycobacterial and
fungal infections, cultures of large volumes from multiple lumbar punctures may be required before an organism is identified
(69,111).
In most bacterial and fungal infections, extraneural sites of possible infection should also be cultured. Depending on the
organism being sought, these sites may include blood, urine, paranasal sinuses, ears, oropharynx, sputum, bone marrow,
prostate, or abscess material.
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Viral Culture
Isolation of viral agents by tissue culture methods has been the traditional means of diagnosis in cases of suspected viral
meningitis or encephalitis. Newer methods of virus isolation have improved diagnostic yield; these include the incorporation
of multiple tissue culture cell lines and a combination of culture and staining procedures (shell vial assay for early antigen
detection, enzyme immunoassays, and immunofluorescence staining). Enteroviruses can be isolated in 43% to 77% of patients,
depending on the predominant viral serotype in a particular community. In approximately half of these cases, virus will be
isolated by day 3 and in more than 80% by day 7 (112). Mumps virus and lymphocytic choriomeningitis virus, the agents of
western and eastern equine encephalitides, may also be recovered from CSF. Herpes simplex virus (HSV) types 1 and 2 can be
isolated from cases of meningitis but are rarely recovered from CSF in cases of encephalitis. Varicella-zoster virus,
cytomegalovirus, and California, St. Louis, and Japanese encephalitis viruses are rarely recovered (113). At present, as is
discussed later, PCR methods have largely replaced tissue culture methods for enteroviruses, Herpetoviridae (herpes simplex,
herpes zoster, cytomegalovirus, Epstein-Barr virus), JC virus, and West Nile virus. West Nile virus meningoencephalitis is
diagnosed largely by serology because the virus is only rarely isolated by tissue culture methods at the time patients present
with neurologic symptoms (114).
Bacterial Infections
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perform these assays in routine laboratories, their high costs compared with bacterial culture methods, and the lack of
available Food and Drug Administration (FDA)-cleared assays.
Mycobacterial Infections
Measurement of Adenosine Deaminase Levels in CSF
Adenosine deaminase is an enzyme that is widely distributed in human tissues and is present in high concentrations in
lymphocytes. Elevation of CSF adenosine deaminase levels may occur in a variety of neurologic disorders, including bacterial
meningitis, brain abscess, neurobrucellosis, cryptococcal meningitis, and CNS lymphoma. Elevated levels of lymphocyte
adenosine deaminase are frequently present in the CSF of patients with tuberculous meningitis, and measurement of this
enzyme has been used to provide presumptive evidence of M. tuberculosis infection and to evaluate response to treatment
(134,135,136). The test, though both sensitive and useful, is not specific, because it detects a component of host response
and does not detect a structural component of the organism itself.
Lyme Disease
The overall sensitivity and specificity of tests for diagnosis of Lyme disease are still being determined, as is the accuracy of
tests used to diagnose CNS involvement. In the United States, the Centers for Disease Control and Prevention (CDC)
recommends using a two-step process for testing serum from patients suspected of having Lyme disease (151). Step one
involves screening with a sensitive assay such as ELISA or immunofluorescence assay. Those samples that are negative by such
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an assay are not tested further. All equivocal or positive results are subsequently confirmed by immunoblotting (Western blot)
(151). Standardized criteria for interpretation of both IgM and IgG are outlined elsewhere (151). Antibody to Borrelia
burgdorferi may be absent early in the course of infection, and seronegative Lyme disease, diagnosed by T-cell proliferation
to Lyme disease, has been reported (152). False-positive test results for Lyme disease may be seen in patients with infectious
mononucleosis, positive serology for syphilis, and autoimmune conditions.
In the acute form of neuroborreliosis, there is usually pronounced synthesis of IgM antibody production (153). IgG and IgA
synthesis is seen in the chronic forms of disease (153). Detection of intrathecal antibody production is considered the most
specific test for neuroborreliosis (154). However, not all patients develop CSF antibodies. The CSF antibody production in
subtle CNS disease is inconsistent and may be lacking in patients with only peripheral nerve involvement (154). Detection of
CSF antibody is not essential for diagnosis (155,156). Accuracy and reliability of tests vary considerably between laboratories;
therefore, positive values reported by laboratories unfamiliar to the physician must be approached with caution and, if
necessary, confirmed.
PCR methods have been extensively studied for their utility in diagnosing Lyme neuroborreliosis, but results have varied
widely, depending on the selection of patients (acute vs. chronic disease), gene targets, PCR methods and controls, and
comparative test results (153,157,158). A recent metaanalysis derived from published PCR results irrespective of methods or
targets from patients with all stages of Lyme neuroborreliosis demonstrated an overall sensitivity of 19% and a specificity of
100% (157). Other reports have indicated that PCR is no more sensitive as a diagnostic tool than the measurement of
intrathecal antibody production and overall is less useful (159,160,161). PCR should not be considered a stand alone test
and a negative result does not rule out neuroborreliosis. At present, molecular assays may, at most, have a limited role as
adjunctive tests in patients who are seronegative and who have a high likelihood of infection (e.g., as in the case of the
immunodeficient patient). As research tools, molecular assays may provide insight into pathogenesis and clinical course when
used prospectively during the course of illness.
Toxoplasmosis
Encephalitis is the most common presentation of toxoplasmosis in the immunocompromised patient and most commonly
results from reactivation of latent infection (173). CSF antibody titers have been used to diagnose and follow CNS infections
caused by Toxoplasma gondii in both patients with AIDS and patients without AIDS (173,174,175). Intrathecal synthesis of
antibodies to T. gondii may be detected in a subset of patients with AIDS with toxoplasmic encephalitis, not all of whom will
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have a demonstrable elevation in serum antibody titers (175). PCR may be useful in the absence of typical serologic or
radiologic studies (173). Current literature reports high specificity and positive predictive value but variable sensitivity
(176,177,178). The latter may reflect variations in primer selections and treatment status of the patients tested
(176,177,178). However, PCR detection could reduce the need for biopsy confirmation of T. gondii infection in patients with
AIDS and other immunocompromised
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patients with negative serum antibody titers and atypical clinical presentations.
Whipple Disease
Whipple disease is a systemic illness caused by T. whippelii. Illness is characterized by a predominance of intestinal
manifestations, but extraintestinal manifestations including endocarditis, myocarditis, pericarditis, and CNS disease occur
with relative frequency (179). Although cultivation of the organism has been reported (180), diagnosis is generally made by a
combination of cytologic analysis of tissue and fluids using periodic acid-Schiff (PAS) staining to demonstrate the presence of
macrophages laden with intracellular organisms and electron microscopy (179). PCR has been helpful in the diagnosis of CNS
and other extraintestinal manifestations, particularly when these are the predominating clinical presentations (179). PCR has
also been used to determine the stage of disease and monitor response to therapy (181). False-positive PCR results have been
reported in asymptomatic individuals, and for this reason, PCR cannot be recommended in place of standard diagnostic
techniques (182).
Viral Infections
Before the advent of PCR, CSF antibody titers and determination of CSF/serum antibody ratios were used as methods of acute
viral diagnosis. Determination of CSF antibody titers per se has been found valuable in the diagnosis of chronic CNS infections
such as tropical spastic paraparesis or subacute sclerosing panencephalitis (183), but CSF antibody titers alone are of limited
value in most cases of acute viral encephalitis. Comparison of serum and CSF titers of IgG and IgM antiviral antibodies has
been proposed as a diagnostic test in encephalitis caused by HSV and other agents, but the test, which is dependent on
intrathecal antibody synthesis, is of limited value at the time of presentation, and intrathecal antibody may become
detectable only as virus is cleared from the CSF compartment (184,185,186,187,188). Most patients presenting with West Nile
virus infection already have CSF IgM antibodies to the virus, making this the diagnostic method of choice (114).
PCR methods have had their greatest impact in the diagnosis of viral meningitis and encephalitis. Despite the lack of
commercially available, FDA-cleared assays, PCR has replaced tissue culture methods in the diagnosis of enteroviral infections
of the CNS and/or as an adjunct to antibody testing for enteroviruses (189,190,191), HSV-1 and HSV-2 (192,193,194,195),
varicella-zoster virus (195,196), cytomegalovirus (197), Epstein-Barr virus (198), JC virus (199), and arboviruses, including
West Nile virus (200,201). Many university, reference, and public health laboratories offer highly sensitive and specific assays.
Physicians ordering these tests should request information regarding the validation of these assays and should be given upon
request data relating to analytic and clinical sensitivity and specificity, as well as inhibition rates. Indiscriminate ordering of
these assays on CSF samples should be discouraged. Triaging of CSF samples to particular assays should be based on
abnormalities of the CSF, seasonality, geographic location, immune status, and other clinical and epidemiologic data, as
suggested by Thomson and Bertram (100).
Compared to viral culture (overall sensitivity, 14% to 24%), the sensitivity of PCR ranges from 75% to 100% depending on the
virus (100,202). Molecular detection of viral nucleic acid sequences in CSF has not only improved diagnosis but also has largely
replaced invasive methods such as brain biopsy, has shortened time to specific diagnosis, and particularly in enteroviral CNS
infections, has proven cost-effective through decreased use of empirical antibacterial therapy and reduction in hospital stay
(189,190,193,194). Finally, molecular assays have added greatly to our understanding of the epidemiology and pathogenesis of
these infections (193,194).
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GLC, with or without mass spectrometry, limit these methods to a few specialized laboratories.
Bacterial Meningitis
Bacterial meningitis characteristically produces a PMN pleocytosis, a depressed level of glucose, and an elevated protein
level. Numbers of PMN leukocytes may vary from a few to many thousand and usually range between 1,000 and 10,000 cells. A
predominantly (>50% of cells) lymphocytic pleocytosis has been reported in up to 14% of patients (208) and may be more
common in meningitis caused by L. monocytogenes infection (209). A predominance of lymphocytes may also be seen in
neonatal gram-negative meningitis (210). Leukocytes may be absent from CSF very early in the course of infection, in
neonatal meningitis, or in severely immunocompromised patients (68,211,212). Glucose levels less than 40 mg/dl or a
CSF/blood glucose ratio less than 0.4 should raise strong suspicion of bacterial meningitis, and a CSF/blood glucose ratio less
than 0.5 should be of concern. Glucose levels less than 18 mg/dl or a CSF/serum glucose ratio of 0.23 makes bacterial
meningitis extremely likely (71,82). Normal glucose values will be obtained in approximately 9% of patients. Protein levels
correspond with the intensity of meningeal inflammation but are between 100 and 500 mg/dl in most patients. Yield on Gram
stain varies from organism to organism (see previous discussion) but is positive in 60% to 80% of untreated patients.
Tuberculous Meningitis
Typical findings in tuberculous meningitis are (a) a pleocytosis with lymphocytic predominance, (b) lowered glucose level, and
(c) elevated protein level (94,95). In approximately 70% of patients, the cell count is between 100 and 400 cells (94).
However, as many as 1,000 to 1,200 cells may be present, and in few patients, the CSF is acellular despite the presence of
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organisms, elevation in protein, and hypoglycorrhachia. Although most cells in the CSF are lymphocytes, relative numbers of
lymphocytes and PMN leukocytes may vary from lumbar puncture to lumbar puncture. Neutrophils predominate in
approximately 27% of patients and are most likely to be present early in the course of infection or in severe infection (97).
Small numbers of cells or acellular CSF may be found in patients with AIDS or those in other states of severe immune
deficiency. Protein levels are 100 to 500 mg/dl in 65% of patients and may reach levels of 1,000 mg or more if treatment is
delayed (94,95). In 25% of patients, protein levels are normal (94). Glucose levels are 30 to 45 mg/dl in 50% of patients and
may occasionally be less than 10 mg/dl. In 17% of patients, CSF glucose levels are normal (94).
M. tuberculosis may be extremely difficult to detect on smear or to recover by culture. When tuberculous meningitis is
strongly suspected, at least 20 mL of CSF should be submitted for acid-fast smear and culture unless contraindicated by
increased ICP; larger volumes (40 to 50 mL) should be submitted if possible. Frequently, the need for a second lumbar
puncture to obtain this large amount of CSF is first suggested by the abnormalities detected in the initial CSF sample. PCR, if
locally available, may provide a rapid means of diagnosis superior to acid-fast stain (142,143,144,145,146). During appropriate
therapy, the CSF glucose level returns toward normal before cell count or protein levels. The CSF cell counts and protein
levels may remain abnormal for protracted periods (94,95,106).
Neurosyphilis
Suspicion of neurosyphilis is predicated on the presence of positive serum rapid plasma reagin (RPR) and fluorescent
treponemal
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antibody-absorption (FTA-ABS) test results. CSF should be sent for Venereal Disease Research Laboratories (VDRL)
determination (see Chapter 37). The CSF may contain variable numbers of lymphocytes and an elevated protein level during
late primary and early secondary stages of the disease and during asymptomatic or symptomatic neurosyphilis (1). The
findings are extremely variable, however, and normal CSF cell count, protein, and glucose values do not exclude active
disease (220,221). Rarely, syphilis may present as an acute meningitis, with CSF findings similar to those of bacterial
meningitis (1). False-negative CSF VDRL determinations that became positive with penicillin therapy have been described in
neurosyphilis associated with AIDS (222). The diagnostic utility of detection of locally produced antibody against the antigens
of T. pallidum by Western blotting and PCR detection of DNA in the CSF is under investigation (223,224).
Lyme Borreliosis
The CSF changes in Lyme neuroborreliosis are typically a mild lymphocytic pleocytosis, modest elevation of protein level, and
normal glucose level. The CSF may be normal, however, or conversely may occasionally exhibit changes identical to those
seen in bacterial meningitis (155,225,226).
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pleocytosis, usually in the range of 10 to 1,000 cells/mm . PMN leukocytes may at times constitute more than 50% of the cells
during the first 24 to 36 hours of the infection (233,234) and in one series were shown to be present for several days (235). In
some patients, with coxsackievirus infections of the CNS, PMN leukocytes may constitute 90% of cells at the onset of
infection, and the predominance of PMN leukocytes may persist for longer than 24 hours. There are also reports of CSF
samples with few or no cells yielding enteroviruses on culture or by PCR (112,236). Protein is elevated in the range of 50 to
100 mg/dl but may sometimes be higher. Glucose is usually normal, but depression of glucose to levels approaching those of
bacterial meningitis has been reported in infections with HSV-2, herpes zoster virus, mumps, and lymphocytic
choriomeningitis virus (70,71,72,73,237,238). Spanos, Harrell, and Durack (82) have developed a helpful nomogram for
distinguishing between bacterial and viral meningitis. However, presumptive antibiotic therapy for bacterial meningitis should
be initiated if the diagnosis of viral meningitis is in doubt. CSF should be sent for PCR analysis for enteroviruses and, if there
is clinical suspicion, for herpes simplex, herpes zoster, and Mycoplasma infection (100,190). Both CSF and serum should be
frozen for future serologic testing.
Requirements for CSF analysis in cases of suspected viral encephalitis are similar to those for viral meningitis, and CSF
findings are often similar. PMN leukocytes may be present in large numbers in severe encephalitides accompanied by
extensive destruction of brain tissue. HSV classically produces a hemorrhagic encephalitis, and small amounts of blood may be
seen in the spinal fluid. However, HSV is not unique in its ability to produce hemorrhagic necrosis of brain, and RBCs are often
not detected; thus, the presence or absence of RBCs cannot be used to differentiate HSV encephalitis from other conditions.
As in viral meningitis, CSF should be sent for PCR and/or viral culture as appropriate, and both serum and CSF should held for
future serologic studies. CSF should be sent for IgM antibody determination in cases of suspected West Nile encephalitis.
AIDS
Abnormalities of CSF in HIV infection are protean and may reflect either (a) a response to CNS invasion by the agent itself, as
in HIV-related meningitis, meningoencephalitis, and encephalopathy, (b) meningitis or parenchymal infection by other agents,
or (c) meningeal reaction to neoplastic or ischemic events within brain or spinal cord. The response to any of these conditions
is often modified by the immunosuppressive effect of the virus (239,240). In HIV-infected individuals, normal findings on
routine CSF studies do not exclude infectious disease of the nervous system. The neurologic complications of HIV infection and
the approach to the patient with suspected neurologic involvement are discussed in detail elsewhere (Chapter 18).
Prion Diseases
Prion diseases do not elicit a cellular reaction in CSF, so the presence of a CSF pleocytosis essentially excludes this group of
diseases. Mild elevation of protein may occasionally be seen (245). In recent years, 14-3-3 protein, S100 protein, tau protein,
and neuron-specific enolase in CSF have been studied as markers for Creutzfeldt-Jakob disease; of these, 14-3-3 protein has
proven most valuable when used in appropriate clinical context. CSF may contain 14-3-3 protein in other neurologic
conditions, however, and its detection is thus not specific for prion diseases (246). CSF from cases of known or suspected
Creutzfeldt-Jakob disease should be regarded as infectious and handled according to current guidelines (247) (see Chapter
17).
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