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OPERATION

MANUAL
LIST NO. 8376-07

ABBOTT LABORATORIES
ABBOTT PARK, IL 60064 U.S.A.
69-6301/R5

ENTIRE CONTENTS COPYRIGHT


ABBOTT LABORATORIES 1988, 1992, 1994
PRINTED IN THE U.S.A.

Revision Log

Revision Log
The Revision Status of the manual is indicated below. Be sure that the
manual contains the latest revision number of all pages. Additional copies
of this manual may be purchased.

Revision Status

Revision

Date

Pages Revised and


Added

Originally Issued

6/88

Not Applicable

41493-102

8/88

All Pages

41493-102/3

9/88

1, 2, 3, 4, 5, 6, 1-16,
1-20, 1-25, 5-51, 6-4,
6-7, 6-16, 6-19, 6-22,
6-24, 9-47, 9-51, 9-55,
10-78, 10-79, 10-80,
10-81, 10-82
Index

69-6301/R5

41493-104

1/90

All Pages

List No. 8376-03

10/90

All Pages

List No. 8376-05

8/91

All Pages

List No. 8376-06

7/92

All Pages

List No. 8376-06

4/94

Sections 2, 5b, 6b, 8,


9a, 10, 10a, 10b, 10c,
Index

List No. 8376-06

9/94

Table of Contents
Sections 1,2
Index

List No. 8376-06

3/95

Sections 1,4

List No. 8376-07

3/03

69-6301/R5

FrontMatter

Cover page

69-6301/R5

Revision Log

iii, iv

69-6302/R2

Section 9

9d-19, 9d-20

IMx System Operation Manual

-iii-

Revision Log

Revision Number*

41493-104
List No. 8376-03
List No. 8376-05
List No. 8376-06

Revision
Software Version

Incorporated By

Date Incorporated

V2.0
V3.0
V5.0
V6.0

*User should record revision number and sign and date this log to provide
a permanent record of revisions.

-iv-

IMx System Operation Manual

69-6301/R5

Section 9
Maintenance

Step 9

As Required Maintenance

Verify that the probe is centered over the cross target.


If the probe position is correct, save the position:
Press [STORE]
If the position is incorrect, press [REPEAT]. Return to Step 4 of the SELECT
Reagent Pack Calibration procedure.

Step 10

Once the reagent positioning pack positions are stored, the boom
assembly parks before beginning the Level Sense Calibration procedure.

Level Sense
Calibration

The Level Sense Calibration sets the vertical height reference point necessary
for the probe/electrode assembly to sense liquid in the following
components:

Reaction cell
FPIA sample cartridge
SELECT reagent packs
The vertical boom reference point is determined with 50 L of diluent buffer
in the sample well of the blank cell. The IMx System automatically calculates
the other vertical reference points from this value.

Step 1

Place unused blank cells into positions #2, #7, #12, #17, and #22 on the
MEIA or SELECT carousel, and lock the carousel.

Step 2

Place the loaded carousel onto the centerpost.

Step 3

Close the access door.

Step 4

Start the Level Sense Calibration procedure.


Press [START]

The MEIA optical assembly verifies the correct placement of the blank
cells.
The dispense system primes several times before dispensing 50 L of
MEIA #2 diluent buffer into the five sample wells.

Step 5

Once the syringes start priming, open the access door.


The probe / electrode assembly lowers and senses liquid in each sample well.
The liquid level value is displayed and printed for each blank cell position.
The five values must be within three steps of each other and within the
optimal range of 260-270 steps.

69-6302/R2

IMx System Operation Manual

9d-19

Section 9
Maintenance

As Required Maintenance

Step 6

Determine whether the Level Sense Calibration is valid.


If the liquid level values are valid, new vertical height reference points are
automatically stored in memory, and the following message is displayed:
STORING CALIBRATION
The liquid levels are invalid if the five values are not within three steps of
each other, or outside of the optimal range of 260-270 steps.

Note

Based on system software settings the message INVALID LEVELS may not be
displayed for levels outside of the 260-270 range.
If the Level Sense Calibration was invalid, proceed to Step 7.
If you have already repeated the Level Sense Calibration and the levels are
still invalid, contact the IMx Customer Support Center; proceed to Step 8.

Step 7

Repeat the Level Sense Calibration.

Remove the MEIA or SELECT carousel from your IMx System.


Empty the MEIA or SELECT carousel over the appropriate waste
receptacle.
Place unused blank cells into positions #2, #7, #12, #17, and #22 on the
MEIA or SELECT carousel.
Lock the MEIA or SELECT carousel and place it onto the centerpost.
Close the access door.
Press [REPEAT].
Return to Step 5 of the Level Sense Calibration.

Step 8

Determine whether to perform FPIA Sample Cartridge procedure.


After Level Sense Calibration is stored, the display window automatically goes
to the FPIA Sample Cartridge procedure, which follows.
If only MEIA Assays are performed on your system, you are finished with
MEIA and SELECT Boom Calibration. Press [EXIT] to return to the Main
Menu.
If FPIA assays are performed on your IMx System, continue to the FPIA
Sample Cartridge Calibration procedure.

9d-20

IMx System Operation Manual

69-6302/R2