Group Members: Siti Farah Wahida binti Ismail (2014773321), Nur Farah Hanim bt Mohd Sahar (2014596757), Nurul

Afiqah Mohd Razali (2014795327),

Fitriani binti Russani (2014983445), Nurul Najwa Jaafar (2014963137)
Lecturer

: Cik Nur Hilwani binti Ismail

Group

: ED2471A

ABSTRACT:

INTRODUCTION:

By conducting this mini project, we can observe the redox reaction by

comparing the catalase activity in chicken liver and carrot in different
condition (temperature and pH). Besides that, we also observed the
cellular respiration in yeast and we also separating and comparing the
chlorophyll pigments in Ipomoea aquatic and Graptophyllum pictum
griff

Catalase is an enzyme found in most living cells and it is very specific in its reaction
(redox reaction), which break down hydrogen peroxide to water and oxygen. Redox
reaction also took place in the photosynthesis and cellular respiration. From
photosynthesis, we can see the redox occur during the photolysis of water. One of the
end products of photosynthesis is carbohydrate which is used in cellular respiration. So
we can relate that redox reaction, cellular respiration and also photosynthesis are
important and relate to each others.

METHODS:
1)

METHODS:

The apparatus was set up as in the pictures

METHODS:
1)

1)

200ml orange juice + 11g dry

yeast

2)

200ml coca-cola + 11g dry

yeast

The apparatus was set up as in the diagram below

3)

1)

5ml of hydrogen peroxide, H2O2 were inserted by

using the syringe into the boiling tube containing
sample acted as catalase.

2)

The boiling tube was secured tightly right after the

H2O2 was inserted and the stop watch was started

3)

The reading of water level in the measuring tube

was recorded in 5 seconds intervals

4)

1)

The apparatus was set up as in the diagram

200ml distilled water + 11g

dry yeast

RESULTS:
Conical flask

Circumferences of the balloons after 5

minutes intervals (cm)
0s

The experiment was repeated with different type of

samples

Orange juice + yeast

Coke + yeast
Distilled water + yeast

15
15
15

5s
21
25
15

10s
24
29
15

15s
30
35
15

20s
30
40.5
15

Types of sample
Fresh chicken liver
Cooked chicken liver
Chicken liver soaked in vinegar
Fresh carrot
Cooked carrot
Carrot soaked in vinegar

A drop of chlorophyll extract (pigment mixture spot) was

dropped and dried. The step was repeated until got 10 drops
of chlorophyll extract at the same point

2)

10 ml of petroleum ether (solvent) had been measured and

poured into test tube

3)

The chromatography paper was placed in the test tube with

the tip of the paper dipped into the petroleum ether

4)

The apparatus was setup for two chlorophyll extracts

(Ipomoea aquatic and Graptophyllum pictum griff extracts)

RESULTS:
Distance moved
by pigment
from origin (cm)

Pigments

Rf values

Distance moved
by solvent
from origin (cm)

Types of
pigments

Ipomoea
aquatica

Graptophyllum
pictum griff

Ipomoea
aquatica

Graptophyllum
pictum griff

Ipomoea
aquatica

Graptophyllum
pictum griff

7.80

7.50

8.00

7.90

0.98

0.95

Carotene

RESULTS:
The Height of Water Level after 5 Seconds
Intervals (ml)

1)

0s

5s

10s

15s

20s

25s

30s

7.00

7.00

8.00

7.90

0.88

0.89

Xanthophylls

10.00
10.00
10.00
10.00
10.00
10.00

9.50
10.00
10.00
9.80
10.00
10.00

8.50
10.00
10.00
9.00
10.00
10.00

7.00
10.00
10.00
8.50
10.00
10.00

5.50
10.00
10.00
7.80
10.00
10.00

4.00
10.00
10.00
6.50
10.00
10.00

2.00
10.00
10.00
6.00
10.00
10.00

4.50

4.00

8.00

7.90

0.56

0.51

Chlorophyll a

3.30

3.50

8.00

7.90

0.41

0.44

Chlorophyll b

3.00

7.90

0.38

Anthocyanin

CONCLUSION:

CONCLUSION:

Catalase reacts with hydrogen peroxide in the catalytic reaction forming O2 and two
molecules of water as its products.

The increasing in circumferences of the balloon was due to the

2H2O2 =2H2O + O2

process. This shown that, the present of sugar in coca-cola and

The fresh chicken liver and carrot has the best catalase reactions as the water level in the
measuring cylinder was gradually decreased. This is due to the formation of oxygen gas by
the catalytic reaction. So, the hypothesis is accepted

orange juice contributes to the cellular respiration of the yeast. So, the

releasing of carbon dioxide by the yeast during the respiration

hypothesis is accepted

REFERENCES:
1) Gerald C.Karp (2014). Cell Biology seventh edition. John Wiley & Sons Singapore Pte. Ltd

2) Retrieved from http://www.biologyjunction.com/paperchromatographyreport.htm

3) Retrieved from http://www.biologycorner.com/worksheets/enzyme_lab.html

4) Retrieved from http://www.scientificamerican.com

Pigment name
Carotene
Xanthophyll
Chlorophyll a

Visible color
Orange
Yellow
Light green

Rf
0.90-0.98
0.85-0.89
0.46-0.56

Chlorophyll b
Anthocyanin

Dark green
Purple

0.40-0.46
0.28-0.38

CONCLUSION:
In this experiment we can see that both of the samples had variety of color and Rf values which
gave different pigments in chlorophyll. In Graptophyllum pictum griff we were able to see an
extra pigment which is Anthocyanins (purple). So, the hypothesis is accepted