Lovely Professional University,Punjab

Format For Instruction Plan [for Courses with Lectures and Labs ]
Course No. BTY150

Course Title:

BIOTECHNOLOGY PERSPECTIVES

L: 1

Course Planner :-

T: 0

P: 3

Cr: 3

13607 :: Sarabjot Kaur

TextBook

1. .) Freifelder D, Physical Biochemistry Applications to Biochemistry and Molecular Biology, W H

Freeman and C., 1982

Other Specific Books

2.

Plummer D T, An Introduction to Practical Biochemistry, Tata Mc Graw Hill Publishing Co, 1988.

Other Readings

Jouranls atricles as compulsary readings (specific articles, Complete reference)

Sr. No.

http://www3.interscience.wiley.com/journal/110544531/home

http://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbt/ijbt0.asp

http://www.nature.com/nbt/index.htm

Relevant Websites

Sr. No.

(only if relevant to the courses)

Salient Features

http://www.biotechnologist2020.com/

Job opportunities in biotechnology

http://learn.sdstate.edu/douglas_raynie/chem434/c hromatography.pdf

Chromatography Basics

http://www.ufs.ac.za/faculties/documents/04/112/B
OC334/Archive/Lecture%206%20Electrophoresis.p df
http://environmentalhealthandsafetyoffice.dal.ca/file s/Betty_Autoclaves_2008.pdf

Basic types of electrophoresis

Page 1 of 5

Web adress

Autoclave instrumentation and basics

Approved for Autumn Session 2010-11

Detailed Plan For Lectures

Week No.

Lecture No.

Topic

Chapters/Sections of
Textbook/other
reference

Homework to be
Assigned to students

Pedagogical tool
Demonstration/case
study/images/anmation
ctc. planned

Part 1
http://lr010.k12.sd.
us/History%20of%
20Biotechnology.p
pt,
http://www.woodro
w.org/teachers/bi/1
993/intro.html

Week 1

Lecture 1

Introduction and History of

Biotechnology

Week 2

Lecture 2

Applications, Limitations and

Future perspectives of
biotechnology

Week 3

Lecture 3

Jobs perspectives in Biotechnology

Week 4

Lecture 4

Introduction to recent
developments in biotechnology

->Reference :1,

Week 5

Lecture 5

Principle of Chromatography

->Reference :1,55-58

Week 6

Lecture 6

Principle of spectroscopy

->Reference :1,99-102

Week 7

Lecture 7

Applications of spectroscopy

->Reference :1,961-965

http://www.biotechi
nstitute.org/what_is
/timeline.html,
http://en.wikipedia.
org/wiki/List_of_bio
technology_compa
nies,
http://helpbiotech.bl
ogspot.com

Part 2
http://www.bio.org/
speeches/pubs/er/ti
meline2.swf

MID-TERM
Part 3
Week 8

Page 2 of 5

Lecture 8

Principle of electrophoresis

->Reference :1,126-128

Class
demonstration of
electrophoretic
apparatus of AGE
and PAGE

Approved for Autumn Session 2010-11

Part 3
Lecture 9

Bacterial Growth curve

->Reference :1,453

Week 10

Lecture 10

Principle of pH meter

->Reference :1,1/1.5/2

Week 11

Lecture 11

Autoclave: Principle,
Instrumentation and working

->Reference :1,10

Week 12

Lecture 12

Surface Sterilization

Week 13

Lecture 13

Introduction to various databases

Week 9

Part 4

Demonstration on
working of
Autoclave
http://www.accesse
xcellence.org/AE/A
EPC/WWC/1993/s
urface.php

->Reference :1,20/1

Spill Over
Week 14

Lecture 14

Recent developments in biotechnology

Week 15

Lecture 15

Agarose gel electrophoresis

Week 16

Lecture 16

NMR spectroscopy

Week 17

Lecture 17

sterilization of microbial media

Scheme for CA:out of 100*

Component

Lab Assessment

Frequency

Out of (If any)

10

11

Marks for each

10

Total Marks

100
100

Total :-

* In ENG courses wherever the total exceeds 100, consider x best out of y components of CA, as explained in teacher's guide available on the UMS
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Approved for Autumn Session 2010-11

*Each experiment of the lab will be evaluated using following relative scheme:

Component

Page 4 of 5

% of Marks

J/E

20

WR

50

VIVA

30

Approved for Autumn Session 2010-11

List of experiments :Expt. No

Title

Equipment Used

individual 1

Principle and demonstration of

paper chromatography

Whatmann paper, solvent system,

Chromatographic Chamber

individual 2

Experimental analysis of BeersLambert law using

spectroscope

Spectrophotometer

individual 3

Principle and demonstration of

colorimetry

Colorimeter

individual 4

Calibration of pH meter and

estimation of pH of different
samples using pH meter

pH meter

individual 5

Principle and demonstration of

Electrophoresis

Electrophoretic apparatus

individual 6

Effect of different temperatures

on bacterial growth

water bath, spectrophotometer

individual 7

Principle and demonstration of

autoclave

Autoclav

individual 8

Principle and demonstration of

Surface sterilization

Surfactant

individual 9

Effect of Various temperatures on

nutrient content of
media

Media, Incubator

individual 10

Introduction of Biological
Database PubMed

Computer System

individual 11

Introduction of Biological
Database - Rebase

Computer System

Reference of Lab manual

Before Mid-Term

After Mid-Term

Page 5 of 5

Approved for Autumn Session 2010-11