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Original Research
The effect of disinfectant agents on dimensional stability and
surface roughness of a tissue conditioner material
Amiralireza Khaledi, Zohre Borhanihaghighi, Mahroo Vojdani
Department of Prosthodontics,
Faculty of Dentistry, Shiraz
University of Medical Sciences,
Shiraz, Iran
Received : 22-02-10
Review completed : 11-05-10
Accepted
: 16-07-10
ABSTRACT
Purpose: The aim of this study was to determine the effect of disinfectant procedures on the
dimensional stability and surface quality of a tissue conditioner used as a functional impression
material.
Materials and Methods: A tissue conditioner (Visco-gel) used as a functional impression
material was disinfected by immersion in 5.25% sodium hypochlorite or 2% glutaraldehyde
solutions for 10 or 20 minutes, respectively. The control group consisted of specimens (n=10)
that were not treated by disinfectants. For testing the dimensional stability, impressions of an
aluminum edentulous arch with three reference points were made. After 24 hours of storage in
distilled water, the specimens were treated by one of the disinfection procedures. Impressions
were poured and, subsequently, 50 stone casts were measured with a Nikon profile projector.
For surface roughness evaluation, disks that contained Visco-gel were pressed against glass
slides. The disks were then stored in distilled water for 24 hours. After disinfection, 50 diskshaped dental stone casts were prepared. Surface roughness values were determined using
a profilometer. For each test, data were subjected to two-way analysis of variance (ANOVA)
followed by Tukeys test ( = 0.05).
Results: The results of the dimensional stability test showed that immersion of Visco-gel
impressions in 5.25% sodium hypochlorite solution or 2% glutaraldehyde solution did not
significantly change the anteroposterior (AP) and cross-arch (CA) distances of the resultant
stone casts when compared to the control group (P>0.05). The surface quality of dental stone
casts formed by Visco-gel did not change significantly when impressions were immersed in the
disinfectant solutions for either 10 or 20 minutes, or when stored in distilled water (P>0.05).
Conclusion: The results showed that the disinfectant solutions used in this study did not have
a significant effect on the surface quality and dimensional accuracy of Visco-gel as a functional
impression material.
Key words: Dimensional stability, disinfection, functional impression, surface roughness, tissue
conditioners
Website:
www.ijdr.in
PMID:
***
DOI:
10.4103/0970-9290.90277
499
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Khaledi, et al.
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Khaledi, et al.
G20
RESULTS
Taking the control group as the baseline we can asses the
influence of treatments on the tested material. Group
mean values of the AP and CA distances of casts made
from Visco-gel impressions, according to time period and
disinfectant solution, and the result of the two-way ANOVA
for dimensional change are shown in Tables 1 and 2,
respectively.
The results for the dimensional stability test demonstrated
that disinfection treatments did not significantly change the
AP and CA dimensions of the resultant stone casts when
compared to the control group (P>0.05). With regard to the
disinfectant and cross-product interaction of disinfectant
time, there were no significant differences between
disinfected vs nondisinfected for both the AP and CA
distances (P>0.05). Results for the time factor indicated
that a significant difference existed for the CA dimension.
According to Tukeys test [Table 3], the only significant
difference was observed between the average value of the
CA distance when the specimens were immersed in 2%
glutaraldehyde for both 10 and 20 minutes (P=0.02).
501
C
H10
H20
G10
AP
CA
AP
CA
AP
CA
AP
CA
AP
CA
Mean
SD
22.20
30.12
22.19
30.09
22.26
30.15
22.13
30.03
22.23
30.21
0.14
0.12
0.10
0.14
0.17
0.13
0.41
0.15
0.14
0.08
95% Confidence
interval for mean
Lower
Upper
bound bound
22.10
22.30
30.03
30.21
22.12
22.27
29.99
30.19
22.14
22.38
30.06
30.25
21.83
22.42
29.93
30.14
22.13
22.32
30.15
30.26
Min
Max
21.90
29.97
22.02
29.75
21.85
29.99
21.08
29.70
21.93
30.09
22.39
30.34
22.41
30.23
22.48
30.41
22.51
30.22
22.37
30.31
Distance
AP
CA
AP
CA
AP
CA
AP
CA
AP
CA
Sum of
squares
0.02
0.00
0.07
0.14
0.00
0.03
2.20
0.72
24648.07
45365.71
df
1
1
1
1
1
1
45
45
50
50
Mean
square
0.02
0.00
0.07
0.14
0.00
0.03
0.05
0.02
P value
0.51
0.00
1.38
8.77
0.05
1.94
0.48
0.97
0.25
0.00*
0.83
0.17
H20
G10
G20
Groups
P value
H20
C
G10
G20
H10
C
G10
G20
H10
H20
G10
G20
H10
H20
C
G20
H10
H20
C
G10
0.80
0.99
0.85
0.25
0.80
0.97
0.23
0.87
0.99
0.97
0.58
0.50
0.85
0.23
0.58
0.02*
0.25
0.87
0.50
0.02*
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Khaledi, et al.
DISCUSSION
Compared with the control, casts made from Visco-gel
impressions and disinfected by immersion were found to
maintain accuracy in both the AP and CA dimensions.
The reproduction of surface quality was also unaffected
by treatments. Therefore, the hypothesis that disinfection
procedures would not significantly affect the dimensional
Table 4: Mean values of surface roughness (m) of casts
made from Visco-gel impressions
C
H10
H20
G10
G20
Mean
SD
0.91
0.88
0.92
0.79
0.67
0.19
0.21
0.17
0.20
0.20
95% Confidence
interval for mean
Lower Upper
bound bound
0.73
0.69
0.76
0.60
0.48
1.09
1.08
1.09
0.97
0.08
Min
Max
0.70
0.60
0.70
0.55
0.35
1.25
1.20
1.20
1.10
0.90
Sum of
squares
0.21
0.01
0.04
1.20
26.2
df
1
1
1
30
35
Mean
square
0.21
0.01
0.04
0.04
P value
5.15
0.22
1.08
0.06
0.64
0.31
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Khaledi, et al.
CONCLUSION
This study tested the effect of 5.25% sodium hypochlorite
and 2% glutaraldehyde solutions on Visco-gel as a
functional impression material. Within the limitations of
this study, it can be concluded that immersion for 1020
minutes does not significantly impact either dimensional
stability or surface roughness of stone casts that have been
formed by Visco-gel.
ACKNOWLEDGMENT
We would like to thank the Office of Vice Chancellor of Research
at Shiraz University of Medical Sciences for providing financial
support.
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