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Federal Register / Vol. 70, No.

242 / Monday, December 19, 2005 / Rules and Regulations 75071

The Commission’s Consumer & Background Reddell and Reyes, respectively. The
Governmental Affairs Bureau, Reference Section 4(b)(3)(B) of the Endangered three Cicurina species are all
Information Center, Shall send a copy of Species Act of 1973, as amended (Act) unpigmented and range in length from
the Order to the Chief Counsel for (16 U.S.C. 1531 et seq.), requires that, 5 millimeters (mm) (0.19 inches (in)) to
Advocacy of the Small Business for any petition to revise the List of 5.6 mm (0.2 in). Gertsch (1992)
Administration. Threatened and Endangered Species distinguished these three species by
Federal Communications Commission. containing substantial scientific and differences he perceived in the female
commercial information indicating reproductive system.
Marlene H. Dortch,
Cicurina cueva, C. bandida and C.
Secretary. listing may be warranted, we make a
reyesi were described by Gertsch (1992)
[FR Doc. 05–24210 Filed 12–16–05; 8:45 am] finding within 12 months of the date of
on the basis of female genitalia of a
receipt of the petition. The finding must
BILLING CODE 6712–01–P small number of specimens. Because
be that the petitioned action is one of there were some locations that only had
the following: (a) Not warranted, (b) records of immature Cicurina that could
warranted, or (c) warranted but that the not be identified to the species level, we
DEPARTMENT OF THE INTERIOR immediate proposal of a regulation contracted Drs. Marshal Hedin and
implementing the petitioned action is Pierre Paquin on September 24, 2004, to
Fish and Wildlife Service precluded by other pending proposals to determine whether species-level
determine whether a species is identification of immature specimens of
50 CFR Part 17 threatened or endangered, and blind Cicurina spiders from southern
expeditious progress is being made to Travis and northern Hays counties
Endangered and Threatened Wildlife add or remove qualified species from could be made using a genetic
and Plants; 12-Month Finding on a the List of Endangered and Threatened assessment technique they had
Petition to List Cicurina cueva (No Species. Section 4(b)(3)(C) of the Act previously applied to other species of
Common Name) as an Endangered requires that a petition for which the Cicurina (see Paquin and Hedin 2004 for
Species requested action is found to be methods). Their report on the contracted
warranted but precluded be treated as study concludes that C. cueva and two
AGENCY: Fish and Wildlife Service,
though resubmitted on the date of such other formally described species, C.
Interior.
finding, that is, requiring a subsequent bandida and C. reyesi (Gertsch 1992),
ACTION: Notice of 12-month petition finding to be made within 12 months.
finding. likely represent variants of a single
Such 12-month findings must be species that shows genetic structuring
published in the Federal Register. across its range. They explain that ‘‘This
SUMMARY: We, the U.S. Fish and
On July 8, 2003, we received a finding makes biological sense, as we
Wildlife Service (Service), announce a
petition requesting that we list a karst would expect geographically-adjacent
12-month finding on a petition to list a
meshweaver, Cicurina cueva (no cave populations to share more genetic
karst meshweaver (spider), Cicurina
common name), as an endangered similarity than caves that are distant in
cueva (no common name), under the
species with critical habitat. On May 25, space. The genetic structuring observed
Endangered Species Act of 1973, as
2004, Save Our Springs Alliance (SOSA) is a natural consequence of the
amended. Since receiving the petition,
filed a complaint against the Secretary fragmented nature of cave habitats, and
both a genetic assessment and a re-
of the Interior and the Service for failure the unique habitat limitations of these
assessment of morphological characters
to make a 90-day petition finding under spiders * * *’’ (Paquin and Hedin
have failed to support the distinctness
section 4 of the Act for C. cueva. In our 2005). The report authors suggest that
of C. cueva from two other named
response to Plaintiff’s motion for rather than three different species, the
Cicurina, C. bandida and C. reyesi. After
summary judgment on October 15, 2004, populations collected represent one
reviewing all available scientific and
we informed the court that we believed species, which they informally refer to
commercial information, we find that
that we could complete a 90-day finding as the ‘‘C. cueva complex.’’ They say
current information available to us does
by January 20, 2005, and if we ‘‘We suggest that conservation activities
not support the taxonomic standing of
determined that the 90-day finding concerning cave populations in this
C. cueva as a species, and therefore it is
provided substantial information that confined geographic region be based on
not a listable entity and listing is
listing may be warranted, we could this single species hypothesis.’’ Since a
therefore not warranted.
make a 12-month finding by December formal revision reflecting this change in
DATES: The finding announced in this 8, 2005. On February 1, 2005 (70 FR taxonomy (the naming and classification
document was made on December 19, 5123), we published a 90-day finding of organisms) has not been published in
2005. and initiation of status review on a a peer-reviewed scientific journal, the
ADDRESSES: The complete file for this petition to list C. cueva as an Service requested independent peer
finding is available for inspection, by endangered species. On March 18, 2005, review of the report. We believe we
appointment, during normal business the District Court for the Western should now make this 12-month finding
hours at the Austin Ecological Services District of Texas, Austin Division, based on the taxonomic treatment
Field Office, 10711 Burnet Rd., Suite adopted our schedule and ordered the recommended in the contracted report
200, Austin, Texas 78758. Please submit Service to issue a 12-month finding on (Paquin and Hedin 2005).
any new information, materials, or before December 8, 2005. Drs. Paquin and Hedin submitted a
comments, or questions concerning this report in May 2005, titled, ‘‘Genetic and
Taxonomy morphological analysis of species limits
species or this finding to the above
address. Gertsch (1992) described and named in Cicurina spiders (Araneae,
C. cueva, C. bandida, and C. reyesi from Dictynidae) from southern Travis and
FOR FURTHER INFORMATION CONTACT: adult, female specimens collected from northern Hays counties, with emphasis
Robert Pine, Supervisor (see ADDRESSES Cave X in 1962 by Bell and Woolsey, on Cicurina cueva Gertsch and
section); 512–490–0057 extension 248. Bandit Cave in 1966 by Reddell and relatives.’’ When Cicurina specimens
SUPPLEMENTARY INFORMATION: Fish, and Airman’s Cave in 1989 by from Travis, Hays, and Williamson

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75072 Federal Register / Vol. 70, No. 242 / Monday, December 19, 2005 / Rules and Regulations

counties, Texas, were compared to Dr. Kirkpatrick is a co-petitioner he was the three taxa into a single species
sampled populations of C. cueva, not considered a peer reviewer. would probably still be the same even
Paquin and Hedin (2005) found that the However, the Service acknowledges his with further genetic analysis.
C. cueva complex (including all three considerable expertise in genetics. To Two reviewers (13, 14) questioned the
named species) forms a monophyletic allow peer reviewers the opportunity to use of particular phylogenetic methods
group (defined as a group descended comment on the issues presented by Dr. to analyze the genetic data and
from a single common ancestral form) or Kirkpatrick, we sent a second request construct the tree diagrams of
clade (a group of organisms that share for peer review to the same twenty relationships. The authors’ present two
features derived from a common scientists on June 20, 2005, and received different trees, or phylogenies, based on
ancestor) within a mitochondrial ten peer reviews (from reviewers 5, 7, 8, a single data set; one generated by
phylogeny (the evolutionary 9, 10, 12, 13, 14, 19, 20). We asked the neighbor joining (NJ) analyses and the
development and history of a species or peer reviewers for their opinion on what other by Bayesian phylogenetics. These
higher taxonomic group based on degree of certainty they would assign to methods differ in that NJ is a distance-
mitochondrial DNA). Additionally, both each of the following hypotheses/ based approach based on analysis of a
C. bandida and C. reyesi are deeply conclusions: (1) C. cueva, C. bandida, matrix of genetic distances (Hedrick
embedded within the mitochondrial and C. reyesi are all one species (Paquin 2000), and Bayesian phylogenetics is a
DNA clade corresponding to the C. and Hedin conclusion), (2) they are all character-based approach (Avise 2004).
cueva complex, indicating that they are separate species, or (3) another Although they rely on different
part of the same group. In addition, they hypothesis/conclusion is possible. We assumptions and may give somewhat
examined female genital morphology asked them to explain their views on different results, both are generally
and found that ‘‘a similar genital appropriate criteria for delimiting accepted methods for analyzing and
morphology, with slight variations, is species using the types of morphological presenting DNA sequence data (Avise
shared across the entire distribution of and genetic data available in this case, 2004), and Avise (2004, page 142)
this species [the C. cueva complex].’’ and how those criteria apply to their recommends that studies include both a
Based on the Paquin and Hedin 2005 review. distance-based approach and a
genetic and morphological results, they Of the 14 peer reviewers that character-based approach for
concluded that these three named taxa responded to one or more requests for comparison. The authors stated that
represent variants of a single species. reviews, 10 reviewers (2, 4, 5, 8, 10, 12, they also analyzed the data using
Ultimately, when C. cueva, C. bandida, 13, 19, 20, and 22) expressed general maximum likelihood analysis, which is
and C. reyesi are formally combined as agreement with Paquin and Hedin’s another character-based method (Avise
a single species, the authors propose all conclusion that C. cueva, C. bandida, 2004). They did not present a
populations within this expanded and C. reyesi represent a single species, phylogenetic tree representing the
species be referred to as C. bandida, as one reviewer (9) expressed support for results of the maximum likelihood
this name has page priority in Gertsch continuing to recognize them as three analysis but stated that the results were
(1992). Paquin and Hedin (2005) separate species, and three reviewers (7, similar to their Bayesian analysis (Dr.
acknowledge that formal taxonomic 14, and 21) concluded that more study Paquin, San Diego State University,
decisions must involve publication in a was needed to distinguish between the pers. comm., 2005; Hedin and Paquin
scientific journal; therefore, the authors one-species and three species 2005). Although we acknowledge that
alternatives. In addition to these overall there are a number of additional
suggest using ‘‘C. cueva complex’’ to
conclusions, most reviewers provided methods of phylogenetic analysis
refer to the morphologically variable
additional comments on various aspects (Hedrick 2000, Avise 2004), the authors
and genetically divergent populations
of the Paquin and Hedin report, and on presented trees representing the two
within this single species until the
pertinent issues related to the major types of trees, as recommended by
formal change is published. In
taxonomic interpretation of genetic and Avise (2004).
consideration of this information for use Three peer reviewers (8, 13, 14)
morphological data. These comments on
in our 12-month finding, we conducted suggested different conclusions could be
specific issues are summarized below.
a scientific peer review of Paquin and Six of the twelve peer reviewers (2, 4, drawn, even if the phylogenies are
Hedin’s 2005 report to determine if the 5, 9, 10, 19) who responded to at least accepted. These alternative
proposed change in taxonomy was one of these two requests for review interpretations reflect differing views on
likely to be accepted. indicated the study overall was well the appropriate amount of genetic
On May 6, 2005, we sent the report to done and the methods used in the difference for delineating species
20 scientists, 19 with Ph.Ds, with genetic aspects of this study were boundaries, which is an active area of
expertise in genetics, morphology, and/ scientifically sound. However, we did debate in taxonomy (Sites and Marshall
or conservation biology for peer review. receive a variety of comments. Below 2004).
We asked that they particularly review we discuss the comments from both of One peer reviewer (14) suggested that
the completeness of the data in the these sets of reviews in regard to the the study of additional morphological
report and identify any pertinent methods, analysis, and conclusions in characters, rather than genitalia, such as
information that may be missing and the the study. somatic (non-sexual) characters, might
soundness of the methodology, data Concerns were raised by five peer find diagnosable differences within the
analysis, conclusions, and reviewers (4, 5, 7, 9, 14) regarding the ‘‘C. cueva complex.’’ However this peer
recommendations in the report. Each authors’ use of a single region of the reviewer doubted that the outcome of
invited reviewer was assigned a mitochondrial DNA. Some believed the such studies would likely affect the
number, which will be referred to here. report would be strengthened by a larger authors’ conclusion that C. cueva is not
We received eight responses (reviewers sample size from each sampling locality, a species. Additionally, one reviewer
2, 4, 5, 7, 8, 10, 13, 14). Dr. Mark inclusion of data from other (14) stated the assessment of genitalic
Kirkpatrick (co-petitioner) also mitochondrial DNA regions, and an variation was subjective and would
submitted two letters to the Service and analysis of genetic markers from nuclear have been better if the different genitalic
personal email correspondence with Dr. DNA. Three peer reviewers (4, 5, 14) parameters could have been quantified
Hedin (regarding the report). Because speculated that the conclusion to group somehow with the variation analyzed

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Federal Register / Vol. 70, No. 242 / Monday, December 19, 2005 / Rules and Regulations 75073

statistically. Reviewers 7 and 12 stated taxonomy presented by Gertsch, the clearly explained their methods and that
that morphology clearly plays a critical alternative taxonomic delineation they are adequate for their questions.
role in deciphering the systematics of presented by Paquin and Hedin (2005) The reviewer also stated that ‘‘Paquin
this group, and reviewer 7 wondered if deserves serious consideration. We also and Hedin have given a conservative
some statistical quantification of note that Paquin and Hedin’s (2005) conclusion based on their data, and
patterns in morphological characters is morphological studies were based on have noted alternative explanations and
possible. Gertsch’s (1992) original more than double the number of the need for more specimens’’.The
diagnoses for these three species specimens available to Gertsch (1992) reviewer stated that ‘‘without more of
included only collection locality and when he originally described the this work I do not see a way to resolve
characters of the female reproductive species. the concerns about data interpretation
system; no other characters were We received a variety of responses to raised by Dr. Mark Kirkpatrick.’’
identified in the diagnosis. The the specific question in the second peer There is ongoing debate among many
diagnosis that accompanies the original review regarding the degree of certainty scientists regarding methods for species
description of a new species is that the reviewer would assign to the differentiation (Sites and Marshall
important because it provides the various hypotheses or possible 2004). Some believe defining species
characters or character states that allow conclusions about species limits. Two boundaries requires a ‘‘total evidence’’
that species to be distinguished from reviewers (8 and 19) clearly supported approach that includes data from
other species. Gertsch (1992) expressed the Paquin and Hedin conclusion that C. multiple genes and morphology, as well
doubts that other characters were useful; cueva, C. bandida, and C. reyesi are all as ecology and behavior. Although it is
for example, ‘‘Cicurella [the subgenus to one species. However, reviewer 8 did
reasonable to believe this debate will
which the species in question belong] disagree about the assignment of three
continue, the Service’s ‘‘Interagency
* * * offer few coloration or somatic or four of the populations to this group
Cooperative Policy on Information
features to allow easy identification.’’ and did differ with Paquin and Hedin
Standards under the Endangered
Gertsch (1992) was also dismissive of about the level of differences accepted
Species Act’’ (59 FR 34271) requires we
the value of different reproductive to represent a species. One of the
use the ‘‘best available comprehensive
features in males and notes that males reviewers (13) was ‘‘unconvinced that
technical information’’ in making
are much less available for study, as the report’s conclusions are correct’’,
Federal listing determinations. The
they represent only a fifth the number and suggested an alternate hypothesis
Paquin and Hedin (2005) report
of mature females. and classification. Reviewers 7 and 9
One reviewer (22) noted variation in believe the Paquin and Hedin provides genetic data for the first time
female genitalia observed among the conclusions should be considered and morphological data based on an
specimens presented in the report was preliminary and premature, increased number of specimens; both
considered ‘‘well within’’ the range of respectively. Reviewers 5, 10, 12, and 20 approaches fail to distinguish C. cueva
intraspecific (within-species) variation tended to accept the Paquin and Hedin from C. bandida and C. reyesi. In
typically observed in female genitalia of hypothesis based on the information addition, the claim by the petitioners
other species and adequately presented; however, they each that the genetic analysis employed is
demonstrates that there is no expressed some uncertainty or not informative about taxonomic
morphological reason to consider C. suggested that additional data collection standing within the C. cueva complex is
cueva, C. bandida, and C. reyesi as three and analysis would be advisable. not supported by the clear
separate species. We recognize that Reviewer 14 felt that both Hedin and correspondence between geography and
study of additional morphological Kirkpatrick provided ‘‘solid, convincing branching patterns of both phylogenetic
characters and more quantitative arguments for their points of view’; this trees. The correspondence between
analysis of current characters could reviewer doubted that further geography and phylogeny indicates that
increase our understanding of investigation would lead to improved the phylogenetic patterns have a
morphological variation within this resolution on the question of how many biological basis and do not simply
group of spiders, but we find little species there are and believes this is present ‘‘noise’’ that is obscuring
support for rejecting the authors’ ultimately a matter of interpretation. biologically important patterns. We
recommended taxonomy, considering In response to divergent opinions believe, based on our review and the
their findings and the peer reviewers’ regarding how to define species limits results of the peer reviews, the Paquin
comments on the morphological data. and how much data are needed to and Hedin (2005) report provides the
Dr. Kirkpatrick thought the Paquin confidently make a species best available information on the
and Hedin (2005) report did not determination, and because some but current taxonomic status of the Cicurina
statistically disprove the ‘‘established not all peer reviewers were familiar complex. Although it is always possible
taxonomy’’ previously described by with spider taxonomy in particular, we that future analyses on other
Gertsch (1992). However, two peer conducted a third peer review. We sent morphological characters or genetic
reviewers (8 and 22) expressed concern four arachnologists the Paquin and markers may convince spider
that Gertsch (1992) did not sufficiently Hedin 2004 publication (that described taxonomists that another taxonomic
account for the possibility of the methods used in this study) and interpretation is appropriate, we cannot
intraspecific variation in genitalic 2005 report, the first peer review request base our findings on the speculative
characters and improperly recognized and responses, Dr. Kirkpatrick’s letters outcomes of studies not yet performed.
minor morphological variants as and emails, and the second peer review We find, however, that the Paquin and
different species and that his species request and responses. We received two Hedin (2005) report is based on
descriptions were based on small responses (reviewers 21 and 22). One of procedures and methods of analysis that
sample sizes. While such a lack of these reviewers (22) stated that ‘‘Based are generally accepted in the application
statistical analysis is common in the on the evidence presented by Hedin & of molecular methods to taxonomy.
field of systematic biology, we believe Paquin, the only well supported Although additional study could affect
that since two experts (19 and 22) in scientific conclusion at this time, is that the taxonomic conclusions of the report,
this field have expressed strong doubts only one species is present.’’ The other according to the requirements of the Act
about the basis of the species-level reviewer (21) stated Paquin and Hedin the best available genetic and

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75074 Federal Register / Vol. 70, No. 242 / Monday, December 19, 2005 / Rules and Regulations

morphological data at this time support Authority: The authority for this action is year was set equal to 18,180,002 lb
the recommendation of Paquin and the Endangered Species Act of 1973, as (8,246,395 kg) (70 FR 303, January 4,
Hedin (2005) to treat these three species amended (16 U.S.C. 1531 et seq.). 2005). The percent allocated to vessels
as one species. Dated: December 8, 2005. landing summer flounder in New York
Marshall P. Jones Jr., is 7.64699 percent, resulting in a
Previous Federal Actions
Acting Director, Fish and Wildlife Service. commercial quota of 1,390,223 lb
Previous Federal actions can be found (630,601 kg). However, the 2005
[FR Doc. 05–24119 Filed 12–16–05; 8:45 am]
in our 90-day finding that published on allocation to New York was reduced to
BILLING CODE 4310–55–P
February 1, 2005 (70 FR 5123), and in 1,374,164 lb (623,317 kg) due to
our notice reopening the comment research set-aside. The states of North
period on August 16, 2005 (70 FR Carolina, New Jersey, and Rhode Island
48093). That information is DEPARTMENT OF COMMERCE
and the Commonwealth of Virginia have
incorporated by reference into this 12- transferred a total of 50,530 lb (22,920
National Oceanic and Atmospheric
month finding. kg) to New York in accordance with the
In addition to information Administration
Atlantic States Marine Fisheries
incorporated by reference we note that Commission Addendum XV to the
the first comment period for providing 50 CFR Part 648
Summer Flounder, Scup, and Black Sea
information for our status review closed [Docket No. 041110317–4364–02; I.D. Bass Fishery Management Plan, bringing
May 15, 2005. Pursuant to 50 CFR 121205C] the total quota to 1,424,694 lb (646,241
424.16(c)(2), we may extend or reopen kg).
a comment period upon finding that Fisheries of the Northeastern United
there is good cause to do so. We States; Summer Flounder Fishery; Section 648.101(b) requires the
reopened the comment period from May Commercial Quota Harvested for New Administrator, Northeast Region, NMFS
23 to June 22, 2005 (70 FR 29471; May York (Regional Administrator) to monitor
23, 2005), since additional information state commercial quotas and to
AGENCY: National Marine Fisheries determine when a state’s commercial
from the genetic analysis of Cicurina Service (NMFS), National Oceanic and
species in southern Travis County was quota has been harvested. NMFS then
Atmospheric Administration (NOAA), publishes a notification in the Federal
completed. Several parties requested Commerce.
another extension of the comment Register to advise the state and to notify
ACTION: Temporary rule; closure. Federal vessel and dealer permit holders
period. We reopened the public
comment period from August 16 to 30, that, effective upon a specific date, the
SUMMARY: NMFS announces that the
2005 (70 FR 48093; August 16, 2005). state’s commercial quota has been
2005 summer flounder commercial
During this final comment period, we harvested and no commercial quota is
quota available to New York has been
made available the results of our peer available for landing summer flounder
harvested and is announcing the closure
review on the Paquin and Hedin (2005) in that state. The Regional
of summer flounder in Federal waters.
report. Administrator has determined, based
Vessels issued a commercial Federal
upon dealer reports and other available
Finding fisheries permit for the summer
information, that New York has
flounder fishery may not land summer
We have carefully assessed the best harvested its quota for 2005.
flounder in New York for the remainder
scientific and commercial information of calendar year 2005, unless additional The regulations at § 648.4(b) provide
available regarding the taxonomic status quota becomes available through a that Federal permit holders agree, as a
of Cicurina cueva. We reviewed the transfer. Regulations governing the condition of the permit, not to land
petition, available published and summer flounder fishery require summer flounder in any state that the
unpublished scientific and commercial publication of this notification to advise Regional Administrator has determined
information, and information submitted New York of the closure and to advise no longer has commercial quota
to us during the public comment vessel permit holders and dealer permit available. Therefore, effective 0001
periods on our status review following holders that no commercial quota is hours, December 14, 2005, further
our 90-day finding. This finding reflects available for landing summer flounder landings of summer flounder in New
and incorporates information we in New York. York by vessels holding summer
received during the public comment DATES: Effective 0001 hours, December flounder commercial Federal fisheries
periods. We also consulted with 14, 2005, through 2400 hours, December permits are prohibited for the remainder
recognized spider and karst invertebrate 31, 2005. of the 2005 calendar year, unless
experts. On the basis of this review, we additional quota becomes available
FOR FURTHER INFORMATION CONTACT:
find that listing C. cueva is not through a transfer and is announced in
Mike Ruccio, Fishery Management
warranted because C. cueva does not the Federal Register. Effective 0001
Specialist, (978) 281–9104.
meet the definition of a ‘‘species’’ under hours, December 14, 2005, federally
the Act. SUPPLEMENTARY INFORMATION:
permitted dealers may not purchase
Regulations governing the summer summer flounder from federally
References Cited flounder fishery are found at 50 CFR permitted vessels that land in New York
A complete list of all references cited part 648. The regulations require annual for the remainder of the calendar year,
herein is available upon request from specification of a commercial quota that or until additional quota becomes
the Field Supervisor at the Austin is apportioned on a percentage basis available through a transfer.
Ecological Services Office (see among the coastal states from North
ADDRESSES section). Carolina through Maine. The process to Classification
set the annual commercial quota and the
Author percent allocated to each state is This action is required by 50 CFR part
The primary author of this document described in § 648.100. 648 and is exempt from review under
is the Austin Ecological Services Office The initial total commercial quota for Executive Order 12866.
(see ADDRESSES section). summer flounder for the 2005 calendar Authority: 16 U.S.C. 1801 et seq.

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