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Microbes are said to be microscopic because some type of microscope is required to see
them.
Metric units are used to express the sizes of microbes. A meter (m) can be divided into 10
decimeters, 100 centimeters, 1,000 millimeters, 1 million micrometers, or 1 billion
nanometers.
The sizes of bacteria and protozoa are expressed in micrometers ( m), whereas the sizes
of viruses are expressed in nanometers (nm).
A typical spherical bacterium (a coccus) is approximately 1 m in diameter. A typical rodshaped bacterium (a bacillus) is about 1 m wide by 3 m long.
Most of the viruses that cause human disease range in size from about 10 nm to 300 nm.
The development of simple and compound light microscopes enabled the discovery and
visualization of microorganisms.
An ocular micrometer is used to measure the dimensions of objects being viewed with a
compound light microscope.
A simple microscope contains only one magnifying lens, whereas a compound microscope
contains more than one magnifying lens.
Total magnification of the compound light microscope is calculated by multiplying the
magnifying power of the ocular lens by the magnifying power of the objective being used.
The limiting factor of compound light microscopes is the type of illumination being used.
Because visible light is used as the source of illumination, objects that are smaller than half
the wavelength of visible light cannot be seen.
The resolving power or resolution of an optical instrument is its ability to distinguish
between two adjacent objects.
The resolving power of the compound light microscope is approximately 0.2 m, which is
about one-half the wavelength of visible light.
When using a brightfield microscope, a person can see objects against a bright
background. When using a darkfield microscope, a person sees illuminated objects against
a dark background.
Electron microscopes enable scientists to observe objects that are too small to be seen with
a compound light microscope. For example, because they are so tiny, most viruses can
only be seen using electron microscopes.
Extremely small objects can be seen using electron microscopes, because electrons are
used as the source of illumination. The wavelength of electrons is shorter than that of
visible light.
Transmission electron microscopes enable scientists to see inside of cells (i.e., to see
internal details).
The resolving power of a transmission electron microscope is approximately 0.2 nm, which
is about a million times better than the resolving power of the unaided human eye and one
thousand times better than the resolving power of the compound light microscope.
Using scanning electron microscopes, scientists are able to study surface details. The
resolving power of the scanning electron microscope is approximately 20 nm.
Photographs taken through the lens system of the compound light microscope are called
photomicrographs, whereas those taken with electron microscopes are called transmission
electron micrographs and scanning electron micrographs.