Reagent

2,4dinitrophenylhy
drazine
(2,4-DNPH)

Structure

Physical Properties
Melting Pt: 202 C
Boiling Pt: Not available
Density: Not available
Appearance: Wet, red or
orange, crystalline powder.

Hazards
Eye: irritation, redness and pain
Skin: Symptoms include redness, itching, and
pain. May cause allergic skin reactions.
Absorption into body may cause cyanosis.
Ingestion: may cause cyanosis
Inhalation: may cause cyanosis

Acetic Acid

Melting Pt: 16.6C

Boiling Pt: 117C
Density: 1.05 g/mL
Appearance: Clear Liquid

Benzophenone

Melting Pt: 49C

Boiling Pt: 305C
Density: 1.11 g/cm3
Appearance: White Solid

Solid
nitrophenol

Melting Pt: 113C

Boiling Pt: 279C
Density: 1.485 g/cm3
Appearance: Yellowishbrown solid

Eye: Causes severe eye irritation. Contact with

liquid or vapor causes severe burns and possible
irreversible eye damage.
Skin: Causes skin burns. Contact with the skin
may cause blackening and hyperkeratosis of the
skin of the hands.
Ingestion: May cause severe and permanent
damage to the digestive tract. Causes severe
pain, nausea, vomiting, diarrhea, and shock. May
cause polyuria, oliguria and anuria. Rapidly
absorbed from the gastrointestinal tract.
Inhalation: Effects may be delayed. Causes
chemical burns to the respiratory tract. Exposure
may lead to bronchitis, pharyngitis, and dental
erosion. May be absorbed through the lungs.
Eye: Causes eye irritation.
Skin: Causes skin irritation. Animal feeding
studies have resulted in liver and bone marrow
damage.
Ingestion: Ingestion of large amounts may
cause gastrointestinal irritation. The toxicological
properties of this substance have not been fully
investigated.
Inhalation: Causes respiratory tract irritation.
Eye: irritation, redness and pain
Skin: irritation, redness and pain
Ingestion: May cause irritation and systemic
poisoning.
Inhalation: May cause kidney and liver damage,
drowsiness, fatigue, slurred speech.

First Aid Measure

Eye: Flush eyes (lower and upper eyelids) with water
minutes.
Skin: Wash skin with soap for 15 minutes. Remove
contaminated clothing/shoes.
Ingestion: If vomiting occurs, keep head lower than h
help prevent aspiration. If person is unconscious, turn
side.
Inhalation: If adverse effects occur, remove to
uncontaminated area. Give artificial respiration if not
breathing.
Eye: Flush eyes with water for 15 minutes.
Skin: Wash skin with soap for 15 minutes. Remove
contaminated clothing/shoes.
Ingestion: If vomiting occurs, keep head lower than h
help prevent aspiration. If person is unconscious, turn
side.
Inhalation: If adverse effects occur, remove to
uncontaminated area. Give artificial respiration if not
breathing.

Eyes: Flush eyes (lower and upper eyelids) with wate

minutes.
Skin: Flush skin with plenty of soap and water.
Ingestion: Do NOT induce vomiting. If victim is consc
and alert, give 2-4 cupfuls of milk or water. Get medic
irritation or symptoms occur.
Inhalation: Remove from exposure to fresh air immed
If not breathing, give artificial respiration. If breathing
difficult, give oxygen.
Eye: Flush eyes (lower and upper eyelids) with water
minutes.
Skin: Wash skin with soap for 15 minutes. Remove
contaminated clothing/shoes.
Ingestion: If vomiting occurs, keep head lower than h
help prevent aspiration. If person is unconscious, turn
side.
Inhalation: If adverse effects occur, remove to

Benedicts
reagent

Melting Pt: Not available

Boiling Pt: 100C (lowest)
Density: 1.11 g/mL
Appearance: Liquid

Eye: irritation
Skin: irritation
Ingestion: may cause tissue damage on mouth
Inhalation: may produce severe irritation of
respiratory tract.

uncontaminated area. Give artificial respiration if not

breathing.
Eye: Flush eyes (lower and upper eyelids) with water
minutes. Get medical attention immediately.
Skin: Wash skin with soap for 15 minutes. Remove
contaminated clothing/shoes. SPEED ACTION IS CRITIC
Ingestion: If vomiting occurs, keep head lower than h
help prevent aspiration. If person is unconscious, turn
side. Give 2-4 cupfuls of milk or water.
Inhalation: If adverse effects occur, remove to
uncontaminated area. Give artificial respiration if not
breathing.