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Introductory Cell Biology Bio113

Review Sheet for Practical #1


Here are a list of questions on various exercises from the labs to help you prepare for the
practical. While I have tried to cover most of what you may see on an exam, I make no promise
that all possible questions are represented on this sheet. You are responsible for everything
that has been covered in lab and all material in the lab notebook.
GENERAL
Know the set-up for each experiment
What compounds were used
What were the instruments and equipment used
What results were obtained
How the data was graphed and interpreted
What organisms were used
Know the concepts that each experiment was demonstrating or testing
Know the answers to the questions on your labs
SDS-PAGE ELECTROPHORESIS
What makes up the gel
Why do we use this type of gel
What did we separate on PAGE gel
How does equipment work
How was protein denatured
How was protein visualized
What were the chemicals that were used in above steps
Set-up of experiment
DNA ELECTROPHORESIS
What is the name of each part of the apparatus?
What is the function of each part?
What is the correct way to set-up the electrophoresis unit for running a gel?
Identify:comb, dam, gel tray, gel box, electrode, power supply,
What is the theory behind gel electrophoresis?
What is the function of loading dye?
UV TRANSILLUMINATOR
What is this piece of equipment called?
What is it used for?
How do you visualize DNA on a gel?
What is the function of the plastic piece on the top?
Why does a gel glow in the dark when it is placed this piece of equipment?
PIPETTOR and PIPETTE
What is the name of this piece of equipment?
What is the maximum volume it will deliver?
What is the minimum volume it will deliver?
What is the volume shown on the dial?
What is the proper method for using it accurately?
What is the function of each white button?
What role does the yellow plastic tip play?
DNA ELECTROPHORESIS Photo of Gel and Graph
Be able to measure a band on a gel and determine its molecular weight using the
standard curve.

Make sure you could draw a standard curve.


Know how to use semi-log graph paper.
Where is the largest fragment located? The smallest?
What direction did the gel run?
BIURET REAGENT
What type of macromolecule does this reagent identify?
Why does it react with this macromolecule and not others?
What does a positive test look like? A negative?
What compound was used as the positive control? The negative control?
IODINE
What type of macromolecule does this reagent identify?
Why does it react with this macromolecule and not others?
What does a positive test look like? A negative?
What compound was used as the positive control? The negative control?
BENEDICTS
What type of macromolecule does this reagent identify?
Why does it react with this macromolecule and not others?
What does a positive test look like? A negative?
What compound was used as the positive control? The negative control?
SUDAN III
What type of macromolecule does this reagent identify?
Why does it react with this macromolecule and not others?
What does a positive test look like? A negative test?
What compound was used as the positive control? The negative control?
ICE FLOATING
What characteristic(s) of water is being demonstrated here?
Explain what you are seeing?
Why does water behave in this manner?
MICROSCOPE SLIDES Polar/Nonpolar
What characteristic(s) of water is being demonstrated here?
Explain what you are seeing?
Why does water behave in this manner?
THERMOMETERS Heat of Vaporization
What characteristic(s) of water is being demonstrated here?
Explain what you are seeing?
Why does water behave in this manner?
CELLS, ORGANELLES, MICROSCOPE
ORGANISMS
Names of the organisms you observed in the lab
What type of organism were they-protists, eukaryotes, plants
Know the different organelles you observed in each organisms
What were the organelles used for movement, obtaining food, storage, and water
movement?
What are the specific components in cilia or flagella and how do they work?
Can you identify and explain the purposes of the different conditions/chemicals you
exposed the organisms to (protoslo, salt solution, water, detergent?
What was the purpose of cytoplasmic streaming? What organism was this observed in?

Can you identify and name the organelles of the organisms used?
MICROSCOPE
Know the name of the parts of the microscope
The magnification and how to calculate the total magnification
What was used to clean the lens and eyepiece?
What objective lens you start off with and the settings you would use when you first look
at a sample?
When oil immersion is used?
DATA for all experiments Make sure that you could look at any of the graphs from the lab and
interpret the results.