Journal of Product Analysis

Identification of Caffeine in Coca-Cola by using HPLC

Methods
Bagas Rinaldy, Kurniawan Cahyo, Randhy Dwi Rendrahadi
Chemical Analysis of Bani Saleh Vocational School
Jl. R. A. Kartini No.66 Bekasi 17113
ABSTRACT
Has conducted research on the levels of caffeine in soft drinks in cans or bottles by using
liquid chromatography method or KCLT high level [1]. most use of caffeine aims to meet
needs in the industry drinks and also used as a flavor enhancer or seasoning on some
food flavoring (Misra et al, 2008) in the present study will establish the caffeine content
using HPLC method
Keywords: caffeine,, HPLC

INTRODUCTION
Along with the increasing
growth in the food and beverage
industry. In soft drinks often added
caffeine, artificial sweeteners and
preservatives whose levels must be
considered because if left unchecked
can cause disease in the human
body[2]. Soft drinks (soft drinks) is
defined as non-alcoholic beverages,
both containing carbonate (soda) or
not containing carbonate. In general,
a soft drink made from water, sugars,
organic acids and other auxiliary
substances, and to drink carbonated
soda is also added. Especially for
carbonated soft drinks cola type
usually also added caffeine [1].
Caffeine
consumption
,
especially in the increasingly popular
among
students
because
of
the
stimulation effect of helping students to
learn hearts long period of time ,
especially when the workload increases
as before a test or exam .

The addition of caffeine in

carbonated soft drinks cola type
intended to evoke a sense of fresh,
pep, also to relieve fatigue and
sleepiness. This can occur because
caffeine has a stimulating effect on
the central nervous system and the

skeletal muscles. In addition, caffeine

also can stimulate the heart and
respiratory system [1].
in this study , using the HPLC
method . Another method used to
determine the caffeine content using
paper chromatography method , but
it is rarely used Considered therefore
are no longer modern and efficient .
so this method is rarely used for
routine analysis [2].
MATERIALS AND METHODS
Method
In this study using HPLC
method using a set of tools HPLC.
there are several ways to define the
levels of caffeine such as using
spectrophotometric
method
,
iodometric
and
paper
chromatography, but because it is
not very efficient for everyday assay.
can use HPLC method
Procedure
Parameter Optimization of HPLC
Measurements
Determination
Motion
Phase
Methanol: Water
As much as 5 nanometers
standard solution of caffeine injected
into
the
HPLC
injector.
The
composition of the mobile phase

used was methanol: water at a ratio

of 100: 0, 90:10, 80:20, 70:30, 60:40,
50:50.
Determination of Rate of Water
At this stage used the
optimum mobile phase composition.
A total of 5 nano liter caffeine
standard solution is injected into the
HPLC injector, with a variety of power
flow rate of 0.5; 0.8; 1; 1.25 and 1.5
ml / min.
Determination of pH
As much as 5 nano-liter
standard caffeine injected into the
injector. Phases are used mainly on
the composition of the phosphate
buffer and the optimum flow rate
obtained in previous studies[2].
RESULTS AND DISCUSSIONS
HPLC optimization
HPLC conditions can vary
obtain optimal analytical results .
chromatographic
conditions
of
varying the ratio of methanol mobile
phase ; water , Euler flow rate , and
the pH of the Euler at 270mm
wavelength
scale
is
done
.
Determination is based on the results
of the summit chromatography ,
because the peak is the most
powerful parameter to measure to
quantitative.
Optimization of the Composition of
the Mobile Phase of Methanol:Water

This study used reverse

phase chromatography (reserved
phase), the mobile phase used more
polar. for the determination of
caffeine content using methanol and
water phase (iew) because caffeine is
soluble in methanol and water.
Lane Optimization Flow Eleun

Mobile phase flow rate ( Euler ) is

very important in chromatography as
instrumental
for
analytical
performance generated . This can be
obtained as follows fast rate, to a
smaller area and a smaller retention
resulting in acquired Time flows [2].
Eluent pH Optimization
Euler pH of the resulting
optimization can be determined
using the mobile phase and the
optimum flow rate that has been
obtained.
CONCLUSION
based on the data it was
concluded that to determine the
caffeine content in the sample can
use
HPLC
method,
some
optimizations performed on HPLC
systems can improve analytical
performance, all of which showed the
effectiveness and sensitivity of the
methods used.
REFFERENCES
[1]
http://www.academia.edu/1267013/Id
entifikasi_dan_Penetapan_Kadar_Kafe
in_dalam_Beberapa_Produk_Minuman
_Ringan_Bersoda_Jenis_Kola_Kemasa
n_Kaleng_yang_Beredar_di_Jatinango
r_Dengan_Metode_Kromatografi_Cair
_Kinerja_Tinggi
[2]http://www.academia.edu/654650
1/OPTIMASI_KINERJA_ANALITIK_PADA_
PENENTUAN_KAFEIN_DENGAN_METO
DE_KROMATOGRAFI_CAIRAN_KINERJA
_TINGGI_THE_OPTIMATION_OF_ANALY
TICAL_PERFORMANCE_FOR_DETERMI
NATION_OF_CAFFEIN_WITH_HIGH_PE
RFORMANCE_LIQUID_CHROMATOGRA
PHY_METHOD