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individual colony. Like the spread plate, the pour plate can be
used to determine the number of cells in a population. Plates
containing between 30 and 300 colonies are counted. The total
number of colonies equals the number of viable
microorganisms in the sample that are capable of growing in
the medium used. Colonies growing on the surface also can be
used to inoculate fresh medium and prepare pure cultures.
MAINTENANCE AND PRESERVATION OF PURE CULTURES
Most microbiology laboratory maintain a large collection of
strains, frequently referred to as a stock-culture collection.
These organisms are needed for laboratory classes and
research work, as test agents for particular procedures, or a
reference strains for taxonomic studies. Most major biological
companies maintain large culture collections. The strains are
used
for
screening
of
new,
potentially
effective
chemotherapeutic agents; as assay tools for vitamins and
amino acids; as agents for the production of vaccines, antisera,
antitumor agents, enzymes, and organic chemicals. For these
and other purposes it is extremely important to have properly
identified and cataloged strains of bacteria available.
Consequently, a considerable amount of research has been
performed to develop methods whereby bacterial strains can
be preserved and stored until they are needed, several
different methods have been developed, since not all bacteria
respond in a similar manner to a specific method. However, all
the methods should have the same objective: to maintain
strains alive and uncontaminated and to prevent any change in
their characteristics.
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