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M. Sc.
In
BIOTECHNOLOGY
(FTPG)
COURSE STRUCTURE
&
DETAILED SYLLABUS
M.Sc. (BIOTECHNOLOGY)
COURSE STRUCTRURE
I YEAR
I SEMESTER
SUBJECT
CODE
MBT-101
MBT-102
MBT-103
MBT-104
MBT-105
MBT-106
MBTL-11
MBTL-12
MBTL-13
MBTL-14
MBTL-15
SUBJECT TITLE
PERIOD
CREDITS
Cell Biology
Biomolecules
Computer Applications and Biostatistics
Microbial Physiology and Genetics
Molecular Biology
Basic Engineering Mathematics
Cell Biology Lab
Biomolecules Lab
Computer Applications and Biostatistics Lab
Microbial Physiology and Genetics Lab
Molecular Biology Lab
TOTAL:
/WEEK
4
4
4
4
4
4
3
3
3
3
4
40
3
3
3
3
3
2
1
1
1
1
1
22
II SEMESTER
SUBJECT
CODE
MBT-201
MBT-202
MBT-203
MBT-204
MBT-205
MBTL-21
MBTL-22
MBTL-23
MBTL-24
MBTL-25
SUBJECT TITLE
Macromolecules and Analytical Techniques
Biology of the Immune System
Process Engineering Principles
Enzyme Technology
Environmental Biotechnology
Macromolecules and Analytical Techniques
Lab
Biology of the Immune System Lab
Process Engineering Principles Lab
Enzyme Technology Lab
Environmental Biotechnology Lab
TOTAL:
II YEAR
PERIOD
CREDITS
/WEEK
4
4
4
4
4
4
3
3
3
3
3
2
4
4
4
4
40
2
1
1
1
22
III SEMESTER
SUBJECT
CODE
MBT-301
MBT-302
MBT-303
MBT-304
MBT-305
MBTL-31
MBTL-32
MBTL-33
MBTL-34
MBTL-35
SUBJECT TITLE
PERIOD
CREDITS
Genetic Engineering
Plant Biotechnology
Animal Cell Science and Technology
Bioprocess Engineering and Technology
Bioinformatics
Genetic Engineering Lab
Plant Biotechnology Lab
Animal Cell Science and Technology Lab
Bioprocess Engineering and Technology Lab
Bioinformatics Lab
TOTAL:
/WEEK
4
4
4
4
4
4
4
4
4
4
40
3
3
3
3
3
2
2
1
1
1
22
IV SEMESTER
SUBJECT
CODE
MBTP-301
SUBJECT TITLE
Research Project
TOTAL:
PERIOD
CREDITS
/WEEK
22
22
22
22
PRACTICALS:
1. Titration of amino acids
2. Determination of pK
3. Reactions of amino acids, sugars and lipids- quantitative reactions
4. Analysis of oils-iodine number, saponification value, acid number.
5. UV, Visible, Absorption spectra.
6. Centrifugation, SDS-PAGE & TLC Silver staining
7. Estimation of inorganic phosphorus by Fiske Subbarow Method
8. Isolation and estimation of protein using various colorimetric and spectrophotometric
methods.
BOOKS
1. Biochemical Calculations, lrwin H. Segel, John Wiley and Sons Inc.
2. General Chemistry. Linus Pauling, W.H. Freeman & Company.
3. Organic Chemistry, DJ Cram and GS Hammond, McGraw Hill.
4. Biochemistry. D Voet and JG Voet, J Wiley and Sons.
5. Physical Biochemistry, D Freifilder, W.H. Freeman & Company.
6. Laboratory Techniques in Biochemistry and Molecular Biology, Work and Work.
7. Understanding Chemistry, CNR Rao, Universities Press. Hyderabad 1999.
8. A Biologists Guide to Principles and Techniques of Practical Biochemistry, K Wilson
& KH Goulding, ELBS Edition, 1986.
9. Tools of Biochemistry by T.G. Cooper.
UNIT-I : Introduction of digital computers: Organization, low and high level languagesComputer Basics, Computer Capabilities, Types of computers, Central Processing Unit,
functions of ALU and control unit (CU), Operating system-Microsoft Office
packages(MS-Word, MS-Excel, MS-Power Point),Algorithm, Flowchart, Program
development steps
UNIT-II :, Basic Structures of C language, C tokens, Data types, declaration of
variables, assigning values, arithmetic, relational and logical operator, increment and
decrement operators, control operator, bit-wise operator, expressions, evaluation, inputoutput operators. IF, SWITCH statement, WHILE, DO-WHILE and FOR statements, One
,Two dimensional arrays, initialization, functions, string variables, declaration, reading,
writing, string handle function, user-defined functions, variables & storage lasses,.
Structures, unions, pointers.
Unit-III : Introduction to statistics :population-sample primary data and secondary data
- graphical and diagrammatic representation of data- Measure of central tendency-Mean,
median and mode-measure of dispersion-range-standard deviation -raw and central
moments-skewness and kurtosis(definition only)-Concept of probability classical and
relative frequency definition of probability-addition and multiplication laws of
probability( without proofs) and examples.
Unit-IV : Concept of Random variables: Probability mass function-probability density
function-probability distribution function( definitions only)- Binomial ,Poisson and
Normal distribution( definitions and statements of properties and examples).Principles of
least square-fitting of straight line-parsons coefficient of correlation and concept of linear
regression.
10
PRACTICALS:
1) Growth Curve of bacteria
2) Factors effecting the growth of microorganisms
3) Determination of thermal death point
4) Determination of antimicrobial activity (Disc diffusion, cup plate method)
5) Determination of minimum inhibitory concentrations
6) Bacterial Transduction Generalised/Specialised
7) Phage lysogeny and lytic cycle using E.coli strains
8) Transfection -GFPs
BOOKS:
1. Microbiology, Michael J Pelczar Jr, E.C.S.Chan and Noel R. Krieg, Tata McGrawHill Edition (5th Edition)
2. General Microbiology 5th Edition Stanier et al.
3. Text of Microbiology, D.K.Maheshwari and R.C.Dubey, S. Chand Publication
Reprint 2009
4. Ananthanarayan and Panikers Text of Microbiology, Edited by C K J Paniker
University Press (3rd Edition) 2008
11
DNA
evidences to prove RNA as hereditary genetic material, Types of RNA- mRNA, tRNA,
rRNA. Organelles DNA (Chloroplast/ Mitochondria).
UNIT II:
replication
DNA damage and
repair-
Types
of DNA damage
repair mechanisms-
12
PRACTICAL:
1. Chromosomal DNA Isolation and quantification
2. Plasmid DNA Isolation and quantification
3. Electrophoresis of nucleic acid DNA & RNA
4. Bacterial Conjugation
5. Bacterial Transformation
6. RNA isolation.
BOOKS
1. Molecular Cloning:A Laboratory Manual, J. Sambrook, E.F. Fritsch and T Maniatis,
ColdSpring Harbor laboratory Press, New York, 2000
2. Introduction to Practical Molecular Biology, P.D. Dabre, John Wiley & Sons Ltd,,
New York, 1988
3. Molecular Biology LabFax, T.A. Brown (Ed.), Bios Scientific Publishers Ltd.,
Oxford, 1991
4. Molecular Biology of the Gene (4th Edition), J.D. Watson, N.H. Hopkins, J.W.
Roberts, J.A.Steitz and A.M. Weiner, The Benjamin/Cummings Publ. Co., Inc.,
California, 1987.
5. Molecular Cell Biology (2nd Edition) J. Darnell, H. Lodish and D. Baltimore,
Scientific American Books, Inc., USA, 1994
6. Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis,M. Raff, K.
7. Roberts, and J. D. Watson. Garland publishing, Inc., New York, 1994
8. Gene VI (6th Edition) Benjamin Lewin, Oxford University Press, U.K., 1998, VCH
Publishers, Inc., New York, 1995
9. Genomes, T.S. Brown
13
2. Engineering mathematics
B.V. Ramana
1. Differential Calculus
Shanthi Narayan
2. Integral Calculus
Shanthi Narayan
References:
14
15
16
17
4. Immuno electrophoresis
5. Quantitative precipitin assay
6. Purification of antibodies
7. Lateral flow diagnosties HCG, malaria, Tuberculosis.
8. Blood grouping Agglutination.
9. Cell identification techniques based on staining
Text Books:
1. Kuby Immunology (Kindt, Kuby Immunology) - Thomas J. Kindt, Barbara A.
Osborne, Richard A. Goldsby, publisher: W. H. Freeman, 2006
2. Immunology- David Male, Jonathan Brostoff, David Roth, Ivan Roitt, publisher:
Mosby, 2006
Reference Books:
1. Fundamental Immunology- William E Paul, publisher: Lippincott Williams &
Wilkins, 2008
2. Immunology, Infection, and Immunity - Gerald B. Pier, Jeffrey B. Lyczak, Lee
M. Wetzler, publisher: ASM Press, 2004
3. Lecture Notes: Immunology, 5th Edition- Ian Todd, Gavin Spickett, publisher:
Wiley-Blackwell, 2005
4. Immunology: A Short Course- Richard Coico, Geoffrey Sunshine, publisher:
Wiley-Blackwell, 2009
5. Cellular and Molecular Immunology- Abul K. Abbas MBBS, Andrew H.
Lichtman MD PhD, Shiv Pillai MD, publisher: Saunders, 2007
18
20
Phosphate
dehydrogenase,
lysoenzyme,
carboxy
peptidase,
21
enzymes Applications of immobilized enzyme in Biotechnology. Enzyme activity in nonaqueous media and then industrial applications.
Riboenzyme and catalytic antibodies- Functional proteins- structure and drug targets
(enzymes and receptors)
TEXT BOOKS :
1. "Biochemical Engineering fundamentals" by J E Bailey and D F Ollis, 2nd ed,
McGraw-Hill (1986).
2. Enzymes: Biochemistry, Biotechnology, Clinical Chemistry by T. Palmer Horwood
Publications 2001.
PRACTICAL
1. Estimation of acid phosphatase from plant/animal sources.
2. Enzyme concentration by salt fractionation.
3. Determination of optimum pH, temperature, Km etc of the enzyme.
4. Extraction of enzyme
5. Dialysis
6. Column Chromatography
7. Activity staining for enzyme
8. Enzyme immobilization by entrapment
22
23
PRACTICALS:
1. Biological Oxygen Demand
2. Chemical Oxygen Demand
3. MPN
4. Coliform bacteria
5. Reduction of BOD & COD after incubation with microbes
24
25
26
27
28
29
30
31
32
UNIT IV: Downstream processing; Introduction, removal of microbial cells and solid
matter, foam based separation, precipitation, filtration, centrifugation, cell disruptions,
liquid-liquid extraction, chromatography, membrane process, drying and crystallization,
effluent treatment and disposal.
UNIT V: Applications of bioprocess-I; Alcohol (Methanol, Butanol, Ethanol, etc),
Acids (citric acid, Acetic acid ,Gluconic acid etc), Solvents (glycerol, Acetone, Butanol
etc).
Applications of bioprocess-II; Antibiotics (penicillin, Streptomycin, tetracycline)
Aminoacids (Lysine, Glutamic acid), Single Cell Protein, Mineral beneficiation & Oil
recovery.
PRACTICAL
1. Study of enzyme kinetics of invertase
2. Effect of pH on enzyme kinetics
3. Enzyme inhibition
4. Enzyme immobilization experiments with help of different methods.
5. PLACKETT BUKMAN DESIGN for Media design
6. Response surface methodology for media design
7. Sodium sulphite oxidation method for determination of mass transfer coefficient
Dynamic gassing method for determination of mass transfer coefficient
8. Growth kinetics in batch culture.
9. Ethanol production from S. cerevesiae
10. Determination of Enzyme activity for cellulase
11. Pretreatment techniques for lignocellulosic Biomass for ethanol production
33
TEXT BOOKS
1) Bailey JE, Ollis DF; Biochemical Engineering fundamentals Year of Publication 1986
2) Blanch HW and Clark DS: Biochemical Engineering Marcel Decker Year of
Publication 1987
3) Introduction to Biochemical Engineering by Dr D G Rao.the Mc Graw Hill companies
34
UNIT II: Biological Databases: Definition, Purpose, Formats, Content, Access - PDB,
Genbank, Swissprot, NRL3D, PIR
DNA Analysis- Strategies for sequencing genomes- Systematic approach, random
approach, EST based approach
Introduction to Whole Genomic analysis, Elements of DNA Sequences, ORF finding,
DNA Motifs and Patterns, Micro-satellite Repeat patterns, Gene Structure (promoter
elements, splicing, termination, etc) Gene Identification: Gene identification methods:
grail, genscan etc
sequence Alignment-
algorithms,
phylograms, cladograms
35
Phylogenetic
studies,
dendrograms,
UNIT IV: Primer on: Proteins- Types, Functions and applications, Protein Sequence
Analysis -Physico-chemical properties of Amino acids, Protein sequence motifs, Signal
and membrane Proteins, Overview of Protein structure - Principles & characterization,
Secondary structure elements, Domains, Folds, Motifs, Overview of Protein Secondary
Structure Prediction Methods
PRACTICALS:
1. Primer design
2. Pair wise Alignment
3. Multiple sequence Alignment
4. Nucleotide and Amino acid level
5. Construction of phylogenetic tree
6. Gene & Promoter finding by methods of different softwares
TEXT BOOKS:
1. Bioinformatics : Genome and sequence analysis by David W Mount.
2. Bioinformatics : A practical guide to analysis of genes and proteins by Baxevanis,
Andreas D Wiley Interscience publishers.
REFERENCE BOOKS:
1. Computational Molecular Biology An Introduction by Peter Clote, Rolf Backofen,
Jhon Wiley & Sons
2. Essential Bioinformatics : by Jin Xiong, Cambridge University Press
36
Structure
Prediction:
Bioinformatics
approach
by Igor
F.Tsigelny
37
38