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st
Genetics 1 exam
<Q> Which of the following oligonucleotides forms the most stable duplex with the
DNA fragment 5GTTGACTGCA3
<C>
<C+>
<C>
<C>
<C>
5CAACTGACGT 3
5TGCAGTCAAC 3
5CAACGGGCGT 3
5TGCGGGCAAC 3
5ACGTCAGTTG 3
<Q> Most of the DNA in the eukaryotic nucleus is packaged in nucleosomes with
<C+>
<C>
<C>
<C>
<C>
histones
ribonuclear proteins
ribosomal proteins
topoisomerases
DNA a,b proteins
<Q> To cut a double stranded DNA molecule at the sequence- specific site AGGCCT,
which enzyme should you use?
<C+>
<C>
<C>
<C>
<C>
1|Page
<C>
B and D
<C+> A and C
<C> A and B
<C> D
<C> B
<Q> An E.coli strain with a mutation that eliminates the AP (apurinic or apyrimidinic)
endonuclease is expected to show :
<C>
<C>
<C>
<C>
<C+>
2|Page
<Q> Place the following components of the chromosome in hierarchal order starting
with the smallest component and ending with the largest.
123456-
solenoid
base pair
nucleosome.
nucleotide
metaphase chromosome.
Condensed scaffold form.
<C+>
<C>
<C>
<C>
<C>
<Q> why some bases could be missed from our DNA? Because of
<C+>
<C>
<C>
<C>
<C>
CH3
CH3
AGGACCACCGTTGGCT
TCCTAGTGGCAACCGA
<C>
Proofreading.
<C>
Excision repair.
recombination.
<C> glycosylation.
3|Page
4|Page
<Q> The DNA in a bacterium is uniformly labeled by growth for several generations on
15
a medium containing N as the sole nitrogen source. DNA isolated from these cells
bands with a higher density than DNA from cells grown on N
15
14
14
the cells grown in N are switched to N - containing medium for one generations
and the DNA is banded on a density gradient, what is the pattern of DNA bands
expected by the mechanism responsible for DNA replication?
N15
N14
b c d e
Controls
<C>
<C>
<C>
<C+>
<C>
A
B
C
D
E
5|Page
<Q> Which of the following repair systems can repair the lesion shown?
<C>
<C>
<C+>
<C>
<C>
mismatch repair.
base excision repair.
nucleotide excision repair.
direct repair by photolyase
uridine glycosidase.
<Q> Which one of the following double-slandered DNA molecules has the lowest
melting** temperature (Tm)?
<C> GCGCGCGC
CGCGCGCG
<C> GCCATGGC
CGGTACCG
<C> GGAATTCC
CCTTAAGG
<C> GAAATTTC
CTTTAAAG
<C+> ATATATAT
TATATATA
6|Page
<Q> Pick the type of mutation illustrated by the following nucleotide change
Wild type ACU AGU UUA GAU UGA
Mutant* ACU AGC UUA GAU UGA
<C+>
<C>
<C>
<C>
<C>
transition mutation.
insertion mutation
deletion mutation
transversion mutation
missense mutation
<C>
<C>
<C>
<C+>
<C>
UAGCT
TGACU
ATCGU
TAGUC
TAGCU
7|Page
5'(GACGAC)
5'(CAGCAG)
5'(CTGCTG)
5'(GTCGTC)
5'(GUCGUC)
Answer: b
Q) Cultured cells from three patients with Xeroderma pigmentosum (XP) were fused in
pairs and analyzed for defects in DNA repair, the cells from patient one are
designated P1, from patient two P2, and from patient three P3, the results from
these tests are shown in the table below, A symbol indicates failure of the
fused cell pair to repair U.V - induced DNA damage and a + symbol indicates that
the fusion restores DNA repair.
Which patients apparently have defects in the same gene that in codes DNA
repair Protein?
P1
+
-
P1
P2
P3
a.
b.
c.
d.
e.
P2
+
+
P3
+
-
Answer = d
8|Page
Q) 8-oxyG causes:
a. G to T transversion.
b. C to G transition.
c. A to G transition.
d. T to G transversion.
e. A to C transversion. Answer = a
Q) Match the type of DNA damage to the mutagen effect of nitrous acid:
a. Depurination of Guanine.
b. G-C to A-T transition.
c. A-T to C-G transversion.
d. Base insertion.
e. double strands breaks.
Answer= b.
9|Page
10 | P a g e
11 | P a g e
Q) Match the positions of the lettered arrows in the gene to the following
statements locations of the 3'- flanking region:
Answer = e
12 | P a g e
13 | P a g e
14 | P a g e