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Chloride
DOC316.53.01017
Method 8113
Test preparation
Sample cell
Cell orientation
Adapter
DR 5000
2495402
A23618
DR 2800
2495402
DR 2700
2495402
DR/2500
2427606
DR/2400
2427606
Quantity
1 mL
2 mL
Deionized Water
10 mL
Chloride
Page 1 of 6
Chloride
Mercuric Thiocyanate method for Chloride
Stored Programs
70 Chloride
Start
2. Prepared Sample:
Fill a sample cell with
10 mL of sample.
3. Blank Preparation:
Fill another sample cell
with 10 mL of deionized
water.
4. Pipet 0.8 mL of
Mercuric Thiocyanate
Solution into each sample
cell.
7. Swirl to mix. An
orange color will develop if
chloride is present.
5. Swirl to mix.
Zero
Chloride
Page 2 of 6
Read
Chloride
Interferences
Table 2 Interfering substances and levels
Interfering substance
Extreme pH
Accuracy check
Required for accuracy check:
TenSette Pipet
Navigate to:
DR 5000
OPTIONS>MORE>STANDARD ADDITIONS
DR 2800
OPTIONS>MORE>STANDARD ADDITIONS
DR 2700
OPTIONS>MORE>STANDARD ADDITIONS
DR/2500
OPTIONS>STANDARD ADDITIONS
DR/2400
OPTIONS>STANDARD ADDITIONS
3. Default values for standard concentration, sample volume, and spike volumes can be
accepted or edited. After values are accepted, the unspiked sample reading will appear in the
top row. See the user manual for more information.
4. Prepare three sample spikes. Fill three 50 mL mixing cylinders with 50 mL of sample. Use the
TenSette Pipet to add 0.1 mL, 0.2 mL and 0.3 mL of 1000-mg/L Chloride Standard Solution,
respectively, to the cylinders and mix each thoroughly.
5. Analyze a 10 mL portion of each sample spike as described in the Mercuric Thiocyanate
method for Chloride test, starting with the 0.1 mL sample spike. Each addition should reflect
approximately 100% recovery.
6. Select GRAPH to view the results. Select IDEAL LINE (or best-fit) to compare the standard
addition results to the theoretical 100% recovery.
Chloride
Page 3 of 6
Chloride
Standard Solution Method
Required for accuracy check:
Navigate to:
DR 5000
OPTIONS>MORE>STANDARD ADJUST
DR 2800
OPTIONS>MORE>STANDARD ADJUST
DR 2700
OPTIONS>MORE>STANDARD ADJUST
DR/2500
OPTIONS>STANDARD ADJUST
DR/2400
OPTIONS>STANDARD ADJUST
3. Turn on the Standard Adjust feature and accept the displayed concentration. If an alternate
concentration is used, enter the concentration and adjust the curve to that value.
Method performance
Program
Instrument
Standard
Precision
95% Confidence Limits of Distribution
70
DR 5000
20.0 mg/L Cl
17.922.1 mg/L Cl
DR 2800
20.0 mg/L Cl
17.922.1 mg/L Cl
DR 2700
20.0 mg/L Cl
17.922.1 mg/L Cl
DR/2500
20.0 mg/L Cl
18.321.8 mg/L Cl
DR/2400
20.0 mg/L Cl
18.321.8 mg/L Cl
Program
Instrument
Standard
Portion of curve
Sensitivity
Concentration change
per 0.010 Abs change
70
DR 5000
DR 2800
DR 2700
20.0 mg/L Cl
1.0 mg/L
0.1 mg/L Cl
10.0 mg/L
0.3 mg/L Cl
20.0 mg/L
0.6 mg/L Cl
1.0 mg/L
0.11 mg/L Cl
20.0 mg/L
45 mg/L Cl
DR/2500
DR/2400
Chloride
Page 4 of 6
20.0 mg/L Cl
Chloride
Summary of method
Chloride in the sample reacts with mercuric thiocyanate to form mercuric chloride and liberate
thiocyanate ion. Thiocyanate ions react with the ferric ions to form an orange ferric thiocyanate
complex. The amount of this complex is proportional to the chloride concentration. Test results are
measured at 455 nm.
Quantity/Test
Unit
Catalog number
100 tests/pkg1
2319800
1 mL
100 mL
2212242
2 mL
200 mL
2212129
Water, deionized
10 mL
4L
27256
Quantity/Test
Unit
Catalog number
each
1970001
varies
50/pkg
2185696
Unit
Catalog number
500 mL
18349
Unit
Catalog number
16/pkg
1425010
1L
2370853
50 mL
189641
100/pkg
69257
100 tests (50 samples and 50 blanks) using 10-mL cells. 50 tests using 25-mL cells.
Required apparatus
Description
Pipet, TenSette, 0.1 to 1.0 mL
Pipet Tips, for TenSette Pipet 1970001
Recommended standards
Description
Chloride Standard Solution, 1000-mg/L Cl
75 mm
108368
pair
2410104
680 g
75765
5/pkg
39133
1000/pkg
2185628
10 mL
1451538
each
1465100
50 mL
245026
each
2196800
99.51
Chloride
Page 5 of 6
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