2015 AAPS Annual Meeting and Exposition

29/10/15 08:52

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Sessions: Wednesday Contributed Paper Posters
W5032 - Topic Emulsion Based on Bullfrog Oil Rana catesbeiana Shaw: Development, Characterization and Stability

University of Iowa
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Drug Delivery-Technol-SMFormulation
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Sessions: Wednesday Contributed Paper Posters

See attached abstract pdf for images.

Authors: Lucas Machado, Federal University of Rio Grande do Norte (Main Author); Everton Alencar, University of Iowa (Presenting Author);
Francisco Xavier, Jr., Federal University of Rio Grande do Norte; Andreza Morais, Federal University of Rio Grande do Norte; Renata Rutckeviski,
Federal University of Rio Grande do Norte; Wogenes Oliveira, Federal University of Rio Grande do Norte; Franceline Reynaud, Federal University of Rio
Grande do Norte; Arnobio Silva, Jr., Federal University of Rio Grande do Norte; Eryvaldo Egito, Federal University of Rio Grande do Norte
Presenting Author: Everton Alencar
Purpose: To develop, to characterize and to evaluate the physicochemical stability of emulsions based on bullfrog oil (Rana catesbeiana Shaw) for topic
application.
Methods: The required hydrophilic-lipophilic balance (HLBr) of bullfrog oil was previously defined (12.1) and a pseudoternary phase diagram designed.
A basic emulsion was developed containing 12% (w/w) of bullfrog oil, 8% (w/w) of surfactants mixture (Tween 20 and Span 80) and 80% of water
(w/w). The topic emulsion was prepared using the following pharmaceutical excipients: Propylene glycol (4.00%), Germall (0.30%), Xanthan gum
(1.00%), distillated water (62.55%) and Tween 20 (5.03%) as aqueous phase and Span 80 (2.97%), Butylhydroxytoluene (0.10%), Cetostearyl alcohol
ethoxylate (8.00%), Isopropyl palmitate (4.00%) and bullfrog oil (12.00%) as oily phase. Both systems were prepared by the phase inversion technique at
70 C using an Ultra Turrax T-18 at 11.000 rpm for 10 minutes. Bullfrog emulsions were characterized by the pH, the droplet size and the zeta potential.
The stability of the systems was analyzed during 90 days through the creaming rate performed by the micro-emultocrit technique (11,500 rpm for 10
minutes) and six freeze/thaw cycles. All results were performed in triplicate and expressed as mean standard deviation.
Results: The creaming rate value of the basic emulsion was 2.4% and the topical emulsion did not show creaming because its high viscosity reduces the
droplets motility and increases the stability of the emulsion. After the freeze/thaw cycles study, phase separation was observed in the basic emulsion on
the second cycle, while the topic emulsion remained stable until the sixth cycle, indicating that the addition of excipients in the topical formulation
enhanced its stability under large temperature variations. Acid pH was observed in both systems after ninety days, however the basic emulsion showed
decrease from the fifteenth day. This was expected due the fatty acid compounds presented on the bullfrog oil. Additionally, the basic emulsion showed a
pH decrease after the fifth day, suggesting microbiological contamination. The addition of excipients in the topic emulsion increased its droplet size twofold compared to the basic emulsion. However, the droplet size of the basic (200 nm 8.93) and the topic emulsion (390 nm 36.44) remained constant
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2015 AAPS Annual Meeting and Exposition

29/10/15 08:52

during the study. The values of zeta potential of the basic emulsion (-32.79 mV 2.90) and the topic emulsion (-25.02 mV 4.33) suggest that these
systems were stabilized by electrostatic forces.
Conclusion: This study demonstrated that it was possible to develop emulsions based on bullfrog oil, masking the bullfrog oil organoleptic characteristics
and producing a topic emulsion with higher stability due the addition of pharmaceutical excipients.
Abstract Link: http://abstracts.aaps.org/Verify/AAPS2015/PosterSubmissions/W5032.pdf
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Wednesday Afternoon Poster Session

1:30 PM5:00 PM Oct 28, 2015

OCCC - Exhibit Hall WA3
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