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0.392
2.02 x 10-4
0.0000625
0.328
0.7
0.6
0.5
intercept of the linear graph and the value is submitted into the y = mx + c to obtain x value
(biomass concentration).
this
experiment,
dilution
of
Bovine
Serum
Albumin
The result obtained can be seen in the Table 1 and it is following the
theory. The absorbance values is increases as the biomass concentration
is increases. The calibration curve of absorbance values versus biomass
concentration is shown in the Figure 1. From the graph, it shows that the
absorbance values is directly proportional to the biomass concentration.
This is because when the higher concentration of solution is used, the
spectrophotometer absorbs more light as the light is not able to pass
through the solution totally.
Furthermore, from Table 2, it shows the calculated value of biomass
concentration for a given unknown sample. During the experiment, a
sample of unknown biomass concentration is provided and the biomass
concentration must be determined. In order to calculate its biomass
concentration, the OD reading of the sample is determined first which is
0.392 A. From the OD reading, biomass concentration is determined from
the calibration curve and the calculation is as follows
y = mx + c where y = 0.392
y = 565.35x + 0.2778
0.392 = 565.35x + 0.2778
x = 2.02 x 10-4 g/ml
The biomass concentration of the sample obtained is 2.02 x 10-4 g/ml which is
deviated to the theoretical value at 0.01 g/ml. The differences between trend lines in
experimental data may due to some errors that occur during the experiment such as the
BSA/IgG dry and NaCl solution might not mixed well and form sediment at the bottom of the
cuvette. However, the error in the result obtained is expected as the calibration curve is not
100% accurate as theoretical calibration curve. The fingerprint at the cuvette also will affect
the result as the light cannot pass through the yeast as it is blocked.
An important feature of a colorimetric protein reagent is that it should have a stable
endpoint, thus allowing color formation to reach a plateau. The samples also should be
incubated at room temperature for no more than 1 hour as the absorbance will increase over
time.