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Detection of DNA
To separate this DNA according the size.
electrophoresis.
Aim:
Hypothesis:
You want to detect your isolated plasmid DNA from exp 7, using
I expect the DNA quantities in samples with the length of plasmid DNA should be 3337 bp and NanoDrop ND1000 what do you mean by Nanodrop? To determine your DNA, you use a DNA ladder + control DNA.
Materials:
You use an other concentration for the control
4X Bromophenol
blue
Sybr Safe
Amount
Concentration
H/P statements
GHS H
Flammable
H226
liquid and
statemen
:
vapor
t
Keep away
from
P210 heat/sparks/ope
:
n flames/hot
surfaces. - No
smoking
Wear protective
gloves/protecti
P280
ve clothing/eye
:
protection/face
protection
GHS P
P233 Keep container
tightly closed
statemen :
Ground/Bond
t
P240 container and
:
receiving
equipment
IF ON SKIN
P303 (or hair): Take
+
off immediately
P361 all
+
contaminated
P353 clothing. Rinse
:
skin with
water/shower
Protection
Waste category
Wear protective
gloves/protective
clothing/eye
protection/face
protection.
Method:
Also write down the changes you made on the protocol (running time, etc.)
Results:
Number the lanes in your picture and show base pairs near the ladder.
Conclusion:
The ratio of absorbance at 260 and 280 nm of the sample 4 was 1.02 compared to the 1.8 which is generally accepted as 'pure' for DNA,
which imputes that sample was impure to some extent and corresponds to the privious statement that is probably contaminated with
DNA. Show this ratio in your hypothesis as well. What size is your plasmid DNA? Could you detect it?