UV-mini 1240 PDF

206-94623E
Oct. 2006
Shimadzu UV Visible Spectrophotometer

UVmini-1240
Shimadzu Visible Spectrophotometer
UVmini-1240V
Instruction Manual
Read the instruction manual thoroughly before you use the

product. Keep this instruction manual for future reference.
Shimadzu Corporation provides this publication "as is" without warranty of any kind, either express
or implied, including, but not limited to the implied warranties of merchantability or fitness for a
particular purpose.
All rights are reserved, including those to reproduce this publication or parts thereof in any form
without permission in writing from Shimadzu Corporation.
Information in this publication is subject to change without notice and does not represent a
commitment on the part of the vendor.
Any errors or omissions which may have occurred in this publication despite the utmost care taken
in its production will be corrected as soon as possible, but not necessarily immediately upon
detection.
1998-2006 Shimadzu Corporation. All rights reserved.
Precautions for Safe Operation

UVmini-1240 is an ultraviolet visible spectrophotometer.
UVmini-1240V is a visible spectrophotometer.
To operate the unit safely, strictly observe the following precautions.
1. Do not use for any purpose other than the above-mentioned types of analyses.
2. Follow the procedures described in the Instruction Manual, otherwise the instrument may not be used safely.
3. Observe all warnings and precautions.
4. Do not disassemble or modify the unit without the express approval of an authorized Shimadzu
representative.
5. For internal repair of the product, contact your Shimadzu representative.
MEANING OF CAUTION SIGNS
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in serious
injury or possibly death.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in minor to
moderate injury or equipment damage.
NOTE
Emphasizes additional information that is provided to ensure the proper use of this
product.
SAFETY SYMBOLS
The following are explanations of the safety symbols and their locations used in UVmini-1240.
Alternating Current. (Backside)
Supply On (On the power supply switch)
Supply Off (On the power supply switch)
Caution, Hot surface (See next page for the location of the label)
Caution, Risk of electric shock (See next page for the location of the label)
Replacement Parts Availability

Replacement parts for this instrument will be available for a period of seven (7) years after the discontinuation of
the product. Thereafter, such parts may cease to be available. Note, however, that the availability of parts not
manufactured by Shimadzu shall be determined by the relevant manufacturers.
UVmini-1240
Warning Labels Indicated on the Unit
WARNING
High Temperature
Light source and light source chamber are very hot. When replacing the light source, be sure to turn
OFF the power and check that the light source is completely cooled.
Warning label
Fig.1 UVmini-1240/V
WARNING
Electric Shock
When replacing the fuse, be sure to turn OFF the power and disconnect the power supply cord.
Warning label
Fig.2 UVmini-1240/V
ii
UVmini-1240
Regulatory Information
For Europe:
The product complies with the requirements of the EMC Directive 89/336/EEC amended by 92/31/EEC, 93/68/
EEC, and Low Voltage Directive 73/23/EEC amended by 93/68/EEC.
Product Name
: UV-Visible Spectrophotometer/Visible Spectrophotometer
Model Name
: UVmini-1240/UVmini-1240V
Manufacturer
: SHIMADZU CORPORATION
ANALYTICAL & MEASURING INSTRUMENTS DIVISION
Address
: 1, NISHINOKYO-KUWABARACHO,
NAKAGYO-KU, KYOTO, 604-8511, JAPAN
Authorized Representative in EU: Shimadzu Europa GmbH

Address
: Albert-Hahn-Strasse 6-10, 47269 Duisburg, F. R. Germany
UVmini-1240
iii
Action for Environment

To all user of Shimadzu equipment in the European Union:
Equipment marked with this symbol indicates that it was sold on or after 13th August 2005,
which means it should not be disposed of with general household waste. Note that our
equipment is for industrial/professional use only.
WEEE Mark
Contact Shimadzu service representative when the equipment has reached the end of its life. They will
advise you regarding the equipment take-back.
With your co-operation we are aiming to reduce contamination from waste electronic and electrical equipment
and preserve natural resource through re-use and recycling.
Do not hesitate to ask Shimadzu service representative, if you require further information.
iv
UVmini-1240
CONTENTS
Chapter 1 Before Using (Installation)
1.1
1.2
1.3
1.4
1.5
1.6
Parts Inspection ................................................................................................................................. 1-2

Installation Site .................................................................................................................................. 1-3
1.2.1 Ventilation ............................................................................................................................. 1-3
1.2.2 Cleaning the Unit .................................................................................................................. 1-3
Connecting Power ............................................................................................................................. 1-4
1.3.1 Power Supply........................................................................................................................ 1-4
1.3.2 Ground.................................................................................................................................. 1-4
1.3.3 Connecting the Power Cable ................................................................................................ 1-4
Turning ON Power and Initialization .................................................................................................. 1-5
Installation Function Check ............................................................................................................... 1-6
1.5.1 Baseline Flatness ................................................................................................................. 1-6
1.5.2 Wavelength Accuracy ........................................................................................................... 1-8
Electro Magnetic Compatibility ........................................................................................................ 1-11
Chapter 2 Construction
2.1
2.2
2.3
2.4
Exterior View ..................................................................................................................................... 2-2

2.1.1 Front and Top Views............................................................................................................. 2-2
2.1.2 Rear Side View ..................................................................................................................... 2-3
Sample Compartment........................................................................................................................ 2-4
Keyboard ........................................................................................................................................... 2-5
Light Source Compartment................................................................................................................ 2-7
Chapter 3 Mode Selection and Shared Operations

3.1
3.2
3.3
3.4
Mode Selection Screen ..................................................................................................................... 3-2

3.1.1 Set Parameters [Params]/[F1] .............................................................................................. 3-3
3.1.2 IC Card [IC Card]/[F2]........................................................................................................... 3-4
3.1.3 File Transfer [F3] .................................................................................................................. 3-4
3.1.4 External Control [F4]............................................................................................................. 3-4
Overview of Various Modes............................................................................................................... 3-5
Operations Shared by Modes ............................................................................................................ 3-7
3.3.1 Save Parameters [SavParam] .............................................................................................. 3-7
3.3.2 Save Curve and Call Curve [SavCurve]/[CallCurv]............................................................... 3-8
3.3.3 Data Management [DataFile].............................................................................................. 3-11
Screen Copy and Data Printout ....................................................................................................... 3-13
Chapter 4 Photometric Mode

4.1
4.2
Measurement Parameter Configuration Screen ................................................................................ 4-2

4.1.1 Set Wavelength .................................................................................................................... 4-2
4.1.2 [T%/ABS] .............................................................................................................................. 4-2
4.1.3 Sample Compartment Control [SmplCmpt] .......................................................................... 4-2
4.1.5 Measurement Screen [SmplMeas] ....................................................................................... 4-3
4.1.6 Auto zeroing [AUTO ZERO].................................................................................................. 4-4
4.1.7 Change Sample No. [Smpl No.]............................................................................................ 4-4
4.1.8 Quantitation by K-factor method [Factor K] .......................................................................... 4-4
Post-measurement Processing ......................................................................................................... 4-5
4.2.1 Data Print and Screen Copy ................................................................................................. 4-5
4.2.2 Data Management [DataFile]................................................................................................ 4-5
4.2.3 Data Display [DataDisp]........................................................................................................ 4-5
UVmini-1240
Chapter 5 Spectrum Mode

5.1
5.2
5.3
5.4

5.1.1 Setting Parameter Items ....................................................................................................... 5-3
5.1.2 Baseline Correction [BaseCorr] ............................................................................................ 5-5
5.1.3 Call Curve [CallCurv] ............................................................................................................ 5-5
5.1.4 Sample Compartment Control [SmplCmpt]........................................................................... 5-5
Measurement..................................................................................................................................... 5-6
Post-measurement Processing.......................................................................................................... 5-7
5.3.1 Read Cursor.......................................................................................................................... 5-7
5.3.2 Enlarge/Reduce [Zoom] ........................................................................................................ 5-7
5.3.3 Peak detection ...................................................................................................................... 5-9
5.3.4 External Transmission [ExtTrans] ....................................................................................... 5-10
5.3.5 Save Curve [SavCurve] ...................................................................................................... 5-11
5.3.6 Waveform printing............................................................................................................... 5-11
Scanning Speed and Data Sampling Interval .................................................................................. 5-12
Chapter 6 Quantitation Mode

6
6.1
6.2
6.3
6.4
Quantitation Mode ............................................................................................................................. 6-2

6.1.1 Measurement Parameters .................................................................................................... 6-3
6.1.2 Sample Compartment Control [SmplCmpt]........................................................................... 6-8
6.1.3 Measurement Screen [SmplMeas]........................................................................................ 6-8
Creating a Calibration Curve ............................................................................................................. 6-9
6.2.1 Inputting concentration.......................................................................................................... 6-9
6.2.2 Entering the Absorbance for a Single Point Calibration Curve ............................................. 6-9
6.2.3 Entering the Absorbance for a Multi-point Calibration Curve.............................................. 6-10
6.2.4 Concentration Table............................................................................................................ 6-11
6.2.5 Calibration Curve Display ................................................................................................... 6-12
6.2.6 Changing the Concentration Table ..................................................................................... 6-13
Unknown Sample Measurement (Quantitation)............................................................................... 6-14
6.3.1 Measurement Screen.......................................................................................................... 6-14
6.3.2 Changing the Sample Number............................................................................................ 6-16
6.3.3 Data Management [DataFile] .............................................................................................. 6-16
6.3.4 Data Display [DataDisp]...................................................................................................... 6-16
6.3.5 Display Equation [Equation]................................................................................................ 6-19
Two/Three-wavelength Calibration method ..................................................................................... 6-20
6.4.1 Two-wavelength Quantitation ............................................................................................. 6-20
6.4.2 Three-wavelength Quantitation........................................................................................... 6-20
Chapter 7 Optional Program Pack

7.1
7.2
Starting the Optional Program Pack .................................................................................................. 7-2

Optional Program Pack Auto-start ..................................................................................................... 7-3
Chapter 8 Utilities Mode

8.1
8.2
Utilities Screen................................................................................................................................... 8-2

Setting Instrument Parameters .......................................................................................................... 8-3
Chapter 9 PC Control
9.1
9.2
vi
Connecting to a PC............................................................................................................................ 9-2

Receiving Commands and Protocol .................................................................................................. 9-3
UVmini-1240
9.3
9.4
9.5
Programming Example ...................................................................................................................... 9-5

Explanation of Commands and Data ................................................................................................. 9-9
Command List ................................................................................................................................. 9-10
Chapter 10 File Transfer

10.1 Connecting to a PC ......................................................................................................................... 10-2
10.2 Operation Method ............................................................................................................................ 10-3
Chapter 11 Maintenance & Checking

11.1
11.2
11.3
11.4
Daily Maintenance & Periodic Maintenance .................................................................................... 11-2

Initialization & Error Display............................................................................................................. 11-3
Troubleshooting ............................................................................................................................... 11-5
Replacing Light Source ................................................................................................................... 11-6
11.4.1 Light Source Specifications ................................................................................................ 11-6
11.4.2 Light Source Replacement Procedure................................................................................ 11-7
11.5 Replacing Fuses ............................................................................................................................ 11-11
11.6 List of Consumable Parts, Spare Parts ......................................................................................... 11-13
Chapter 12 Specifications
12.1 Hardware Specifications .................................................................................................................. 12-2
12.2 Software Specifications ................................................................................................................... 12-4
Appendix Optional Accessories

A-1
A-2
A-3
Sample Module (Multi-cell, Sipper Operation) ................................................................................... A-2

A.1.1 Multi-cell Sample Compartment (6-position)........................................................................ A-2
A.1.2 CPS-240 .............................................................................................................................. A-3
A.1.3 Sipper 160 ........................................................................................................................... A-4
A.1.4 Syringe Sipper ..................................................................................................................... A-5
A.1.5 About the Blank Correction Function ................................................................................... A-6
Printer ................................................................................................................................................ A-8
A.2.1 Installation Procedure .......................................................................................................... A-8
A.2.2 Operating Procedure ......................................................................................................... A-10
List of Cells ...................................................................................................................................... A-11
UVmini-1240
vii
This page is intentionally left blank.
viii
UVmini-1240
1
1 Before Using (Installation)
1
1
Chapter 1 Before Using

(Installation)
1
1
1
1
CONTENTS
1.1
1.2
1.3
1.4
1.5
1.6
Parts Inspection ................................................................................................................................. 1-2

Installation Site .................................................................................................................................. 1-3
1.2.1 Ventilation ............................................................................................................................. 1-3
1.2.2 Cleaning the Unit .................................................................................................................. 1-3
Connecting Power ............................................................................................................................. 1-4
1.3.1 Power Supply........................................................................................................................ 1-4
1.3.2 Ground.................................................................................................................................. 1-4
1.3.3 Connecting the Power Cable ................................................................................................ 1-4
Turning ON Power and Initialization .................................................................................................. 1-5
Installation Function Check ............................................................................................................... 1-6
1.5.1 Baseline Flatness ................................................................................................................. 1-6
1.5.2 Wavelength Accuracy ........................................................................................................... 1-8
Electro Magnetic Compatibility ........................................................................................................ 1-11
1
1
1
1
UVmini-1240
1-1
1.1
1.1
Parts Inspection
This instrument is shipped with the following items. Upon opening the shipping container, confirm that all of the
listed parts are accounted for in your shipment.
Table 1.1 Standard Contents

Description
1
1
1
1
1
Spectrophotometer (One of the following)

UVmini-1240 for 100V to 120V sites
for 220V to 240V sites
UVmini-1240V for 100V to 120V sites
for 220V to 240V sites
206-24000-92
206-24000-38
206-54000-92
206-54000-38
Standard Accessories (One of the following)

For 100V to 120V sites
For 220V to 240V sites
206-89613-91
206-89613-92
2-1
2-2
AC Power Cable
Fuses
1
3
Part No.
Instruction Manual
Qty
Comments
071-60816-12
or
071-60825-51
072-02004-22
or
072-02004-19
206-94623
(for 100V to 120V)

(for 220V to 240V)
4.0A (100V to 120V)
2.0A (220V to 240V)
1
1
1
1
1
Fig. 1.1 Standard Contents
NOTE
A thin protective film has been applied to the surface of the keyboard. This film will not be a hindrance if the
keyboard is used with it in place, but if the film begins to peel during use and makes the keyboard difficult to
see, peel it entirely from the surface.
1-2
UVmini-1240
1.2
1.2
Installation Site
In order to fully utilize the features of this instrument and to be able to use it for a long time in a stable condition,
please install it in a location that meets the following conditions.
Any deterioration in function or mechanical damage that occurs as the result of use in a location that differs from
these conditions will not be covered by the warranty, even if they occur within the warranty period. Please take
care beforehand.
Room temperature during use of 15 to 35C.

Out of direct sunlight.
No strong vibration, or continuous weak vibration.
No strong magnetic fields or electromagnetic fields.
Humidity of 45 to 80%. (If the room temperature is 30C or higher, the relative humidity must be no
more than 70%.)
No corrosive gases, or organic or inorganic gases with absorptivity in the ultraviolet range.
Low amounts of dust.
1
1
1
The dimensions of the UVmini-1240/V are 416 mm 379 mm 274 mm (W D H). The minimum floor space
required for installation is 600 mm 480 mm (WLD). In addition, do not place anything in front of the fan on the
left side as it may hinder ventilation.
The UVmini-1240 weighs 11kg. Install the unit on a flat surface that will support this weight.
1
1
1.2.1 Ventilation
When using organic solvent for samples, be sure to maintain sufficient ventilation.
1.2.2 Cleaning the Unit

Keep the main unit cover and the interior of the sample compartment clean for use. To clean them,
moisten a piece of soft cloth slightly with water or weak detergent. Avoid wiping the unit with a very
wet cloth or dropping liquid inside the sample compartment.
1
1
CAUTION
Do not drop liquid such as water and organic solvent. Electrical failure or mechanical failure may be caused.
1
1
UVmini-1240
1-3
1.3
1.3
Connecting Power
1.3.1 Power Supply
The power consumption of this instrument is 160VA. Please use a power supply with a capacity of
160VA or greater. The allowable voltage fluctuation range is 10%. If the voltage fluctuates more than
10%, please use a voltage stabilizer.
1.3.2 Ground
The power cable for this instrument is a 3-wire type which includes a ground wire.
Be sure to plug the power cable in the 3-wire type outlet for grounding.
NOTE
If the electrical outlet is of the 2-wire type, be sure to ground the instrument from the earth terminal of the
power cable.
1.3.3 Connecting the Power Cable

(1) Check to see that the power switch on the unit is OFF (so that the { is pushed in).
(2) Check the power supply voltage indicated on the rear of unit.
(3) Insert the enclosed power cable into the power connector on the left side of the unit.
(4) Insert the power cable into the outlet.
(5) Turn ON the unit power switch.
1
1
The fuses are mounted inside.
1
1
Fig. 1.2 Power Supply Connector
1-4
UVmini-1240
1.4
1.4
Turning ON Power and Initialization
When the power is turned ON, the spectrophotometer is checked and initialized according to the items shown in
the screen in Fig. 1.3. The time required for this initialization is approximately 6 minutes if all of the items are
properly completed. Each item is initialized in order. Then, when the initialization for that item is properly
completed, "OK" will appear. However, if any abnormality is detected, "NG" will appear. Please refer to "11.2
Initialization & Error Display" in Chapter 11 "Maintenance & Checking" regarding the content of the checks
performed for each item and the points to check if an error is indicated.
1
1
1
1
1
UVmini-1240
UVmini-1240V
Fig. 1.3 Initialization screen

The following message will appear at the time the Mode Selection screen is displayed when the unit is used for
the first time or the conditions to select a light source have been changed.
"Baseline data is invalid. Press [RETURN] key and go to Spectrum mode to correct baseline."
This message disappears when the [ENTER] key or the [RETURN] key is pressed, but unless baseline
correction is performed, it appears every time the display returns to the Mode Selection screen. When
performing measurement with one wavelength such as the one in the Photometric mode, 0 Abs (100%)
correction is performed by pressing the [AUTOZERO] key. This makes measurement possible. However, it is
recommended to perform baseline correction once for the optimum 0 Abs correction. Perform the operation
procedures from 1 to 9 described in the next section (1.5.1). When turning ON power after that, the above
message is not displayed as this baseline is memorized.
As the baseline is not stabilized just after completion of initialization, perform baseline correction at least 10
minutes after the completion of initialization.
During initialization after power-on, the sipper unit may occur due to an error, such as insufficient lamp energy
when,
An inappropriately mounted flow cell prevents the light beam from going through the center of the
cell and correctly entering the detector, or
The beam is refracted by the remaining part of a sample in the cell.
In these cases, fill the flow cell with distilled water and turn the unit on again. A message to sip distilled water
will be displayed for five seconds after filter initialization. Press the [ENTER] key while this message is
displayed enable sipping of the distilled water.
Generally, a dry flow cell allows contamination to adhere to the flow cell inner wall, consequently easily allowing
bubbles to be created. Therefore, after cleaning the flow cell, turn off the spectrophotometer power with the cell
filled with distilled water. Follow the procedures above after a measurement: no problem occurs during
initilization.
UVmini-1240
1
1
1
1
1
1-5
1.5
1.5
Installation Function Check
Once installation is complete, check the function of the following items. In the event that a function(s) does not
fulfil the specification listed below due to vibration or shock during shipment, immediately notify the nearest
service representative.
1.5.1 Baseline Flatness

Procedure
Table 1.2 Procedure (Baseline Flatness)
Step
1
Operation, Monitor Display
Key Operation
Turn ON power (Let sit for 60 minutes after automatic

initialization)
1
1
1
1
1
Select "2. Spectrum"
[2]
Set the measurement mode to "ABS"

(Absorbance mode).
Enter [1] until "ABS" is displayed.
Set the scan range to 1100 ~ 200 nm

(For the visible spectrophotometer model, set the scan
range to 1100 ~ 325 nm.)
UV-VIS: [2][1][1][0][0][ENTER]
[2][0][0][ENTER]
VIS :
[2][1][1][0][0][ENTER]
[3][2][5][ENTER]
Set the photometric range to -0.01 ~ 0.01Abs.
[3][-][0][. ][0][1]
[ENTER][0][ .][0][1][ENTER]
Set the scan speed to "Fast".
[4][
1
1
1
or
][ENTER]
* continued.
1-6
UVmini-1240
1.5 Installation Function Check
Step
Key Operation
1
1
1
9
Perform baseline correction.(approx. 6 minutes)
[F1]
10
Start measurement.
[START/STOP]
1
1
11
1
1
1
1
12
When baseline measurement restarts, press the [START/

STOP] key again. When you press the [RETURN] key, the
process will return to Step 8.
Normal Specification
Baseline flatness should be within 0.010Abs, not including shock noise.
1
1
1
UVmini-1240
1-7
1.5.2 Wavelength Accuracy
Measurement Procedure
Start from Step 4 of the "Baseline Flatness" procedure.
Table 1.3 Procedure (Wavelength Accuracy)
1
Step
Set the measurement mode to "E".
Enter [1] until "E" is displayed
Set the wavelength range to 660 ~ 650 nm.
[2][6][6][0][ENTER]
[6][5][0][ENTER]
Set the recording range to 0 ~ 150E.
[3][0][ENTER][1][5][0][ENTER]
Set the scan speed to "Very slow".
[4][
Set the gain to 3.
[7][3][ENTER]
Set the light source selection to the D2 lamp.
[8][
Start measurement.
[START/STOP]
1
1
1
Key Operation
or
or
][ENTER]
][ENTER]
1
1
1
1
1
8
9
* continued.
1-8
UVmini-1240
Step
10

Perform peak detection.
Key Operation
[F2]
11
1
1
1
1
12
Find the difference between the wavelength of the peak

found and 656.1 nm. Within 1.0 nm.
13
Return to the Parameter Setting screen.
[RETURN],[RETURN]
14
Set the wavelength range to 490 ~ 480 nm.
[2][4][9][0][ENTER][4][8]
[0][ENTER]
15
Set the recording range to 0 ~ 30E.
[3][0][ENTER][3][0][ENTER]
1
1
1
16
1
1
1
1
17
Start measurement.
[START/STOP]
1
* continued.
UVmini-1240
1-9
Step
Key Operation
18
1
1
1
19
Perform peak detection.
20
1
1
1
1
1
21
Find the difference between the wavelength of the peak

found and 486.0 nm. Within 1.0 nm.
Normal Specification
Within 1.0 nm
1-10
UVmini-1240
[F2]
1.6
1.6
Electro Magnetic Compatibility

1
NOTE
Descriptions of this section are only applied to the model for EU (European Union) market: 206-55658-38,
206-55659-38.
This instrument complies with European Standard EN61326-1 which is amended in 1998.
Emission: Non-industrial location
Immunity: Industrial location
1. Electro Magnetic Emission

This instrument can be used in domestic environments.
The test specifications are stated below.
1.
Radiated Emission (EN61326-1: 1997 + A.1: 1998 Class B)
2.
Conducted Emission (EN61326-1: 1997 + A.1: 1998 Class B)
3.
Harmonics AC Mains (EN61000-3-2: 1995/A1: 1998/A2: 1998)
4.
Voltage Fluctuation on AC Mains (EN61000-3-3: 1995)
1
1
NOTE
When an electro magnetic disturbance occurs to those instrument being used close to this product, take
appropriate distance between instruments to eliminate the disturbance.
1
2. Immunity to Electro Magnetic Interference
This instrument can be used in industrial environments.
The test specifications are stated below.
1.
Radiated Susceptibility (EN61000-4-3: 1996)

Field strength: 10V/m, Frequency range 80-1000MHz, Modulation: 1kHz AM80%.
2.
Power Magnetic Fields (EN61000-4-8: 1993)

Magnetic field strength: 30A/m
3.
Electrostatic Discharge (EN61000-4-2: 1995)

Charge Voltage: 4kV (Contact), 8kV(Air Discharge)
4.
Fast Transients on AC Power Lines (EN61000-4-4: 1995)

Test Voltage: 2kV
5.
Surge Immunity of AC Power Lines (EN61000-4-5: 1995)

Test Voltage: 1kV line to line, 2kV line to earth
1
1
1
1
6.
Conducted Radio Frequency Immunity of AC Power Lines (EN61000-4-6: 1996)

Applied Voltage: 3V, Frequency Range 150k-80MHz, Modulation: 1kHz, AM80%
7.
Immunity Against Voltage Dips and Interruptions on AC Power Lines (EN61000-4-11)
UVmini-1240
1-11
1.6 Electro Magnetic Compatibility
NOTE
Compliance to the standard does not ensure that the instrument can work with any level of Electro Magnetic
Interference stronger than the level tested.
Interference greater than the value specified above may cause malfunction of the instrument.
To avoid electro magnetic disturbance, it is recommended that you observe the following notices.
(1) Before touching the instrument, discharge any electrostatic charge in the operator's body by touching a
metallic structure connected to the ground.
(2) Do not install this instrument in environments where strong electro magnetic fields are generated
nearby.
1
1
1
1
1
1
1
1
1
1-12
UVmini-1240
2
2 Construction
Chapter 2 Construction
2
2
2
2
CONTENTS
2.1
2.2
2.3
2.4
Exterior View ..................................................................................................................................... 2-2

2.1.1 Front and Top Views............................................................................................................. 2-2
2.1.2 Rear Side View ..................................................................................................................... 2-3
Sample Compartment........................................................................................................................ 2-4
Keyboard ........................................................................................................................................... 2-5
Light Source Compartment................................................................................................................ 2-7
2
2
2
2
2
2
UVmini-1240
2-1
2.1
2.1
Exterior View
2.1.1 Front and Top Views
2
2
2
2
2
2
2
2
2
Fig. 2.1 Front and top views of UVmini-1240/V
2
1
LCD Unit
This displays the operation menus and measurement results, etc. This is a 320 x 240 dot
resolution, backlit LCD unit. You may adjust the contrast for optimum visibility.
Keyboard
This is the input component for giving operation commands and numeric values to the
instrument.
See 2.3 "Keyboard" for detailed instructions.
Sample Compartment
This is the component in which the sample being measured is set. See 2.2 "Sample
Compartment" for details.
Sample Compartment Set Screws (knurled thumbscrews)

These are screws for fastening the sample compartment unit.
IC Card Slot
This is the slot for inserting optional IC cards (Data Pack, Program Pack).
Eject Button
This is the button to eject an IC card.
2
2
2-2
UVmini-1240
2.1 Exterior View
2.1.2 Rear Side View
2
2
2
2
2
2
2
Fig. 2.2 Rear side view of UVmini-1240/V
1
Power Switch
This is the switch for turning the unit ON/OFF.
ON: when I is pushed in.
OFF: when { is pushed in.
Fuse Holder
Use two 4.0A fuses for the 100V to 120V range, two 2.0A fuses for the 220V to 240V range.
AC Power Connector
Connect the enclosed AC power cable to supply power from an AC electrical outlet.
RS-232C Connector
This is a standard RS-232C interface. This can be used to connect an optional printer or a
computer equipped with a standard RS-232C interface.
Attachment Connector (I/O 1)

This is the interface for connecting the optional "Auto Sample Changer (ASC-5)" or "Cell
Positioner (CPS-240A)" accessory.
Attachment Connector (I/O 2)

This is the interface for connecting the optional "Sipper 160" accessory or "Syringe Sipper"
accessory.
Printer Connector (CENTRONICS)

This is the interface for connecting an optional printer.
UVmini-1240
2-3
2.2
2.2
Sample Compartment
2
2
2
2
2
2
2
2
Fig. 2.3 Sample compartment
2
1
Cell Holder
The cell holder for the rectangular 10mm light path cells is mounted.
Cell Holder Set Screws

The cell holder can be easily removed by loosening the cell holder set screws.
Front Board, Sample Compartment

When using a flow cell, etc., holes are needed to pass tubing, etc. through. Therefore, this "Front
Board, Sample Compartment" can be removed and exchanged with different types of front
panels.
(When mounting another front board, another sample compartment unit is required.)
Multi-cell Holder Drive Connector

This is the connector for driving the optional "Multi-cell Holder" accessory.
2
2
2
2-4
UVmini-1240
2.3
2.3
Keyboard
2
2
2
2
2
2
Fig. 2.4 Keyboard
The keyboard is shown in Fig. 2.4. The modes and settings in the various screens can be selected
using the number keys 0 through 9 or the function keys F1 through F4. When selecting modes or
settings, it is not necessary to press the ENTER key after you have pressed the number keys or
function key. On the other hand, when entering numeric values, such as wavelength settings or
display mode, etc., you must press the ENTER key to confirm that value.
Each Key's function is described below.
1
3
4
2
2
START/STOP Key
This is the key for starting and stopping measurement once parameter setting has been
completed.
AUTO ZERO Key

When you press this key, the current wavelength will automatically be set to 0Abs (100%T).
Make sure that prior to sample measurement a blank cuvette is placed in both sample and
reference sides.
2
2
GOTO WL Key
This is the key that is used to change the current wavelength.
ENTER Key
When you enter a value, press this key after the value to set the entering value.
Cursor Keys (
(-),
,
,
)
Use these keys to select an item and to move the cursor in the LCD screen left or right. The left
cursor key can also be used to enter a negative (-) value when entering numeric values.
Function Keys (F1 through F4)

These are the keys corresponding to the functions that are displayed at the bottom of the LCD
unit screen.
UVmini-1240
2-5
2.3 Keyboard
2
2
2
2
RETURN Key
Use this key to return to the screen preceding the current screen.
LCD CONT Key

Use this key to adjust contrast of the LCD display. Press the cursor keys (
held down to change the contrast.
) with this key
PRINT Key
Use this key to output a hard copy of the monitor screen.
Numeric Keys
Use these keys to enter numeric values.
CE Key
Use this key to clear a numeric value entry. When you press this key, the numeric value which
has been entered will be cleared and then you may re-enter the appropriate value.
2
2
2
2
2
2
2
2
2-6
or
UVmini-1240
2.4
2.4
Light Source Compartment

2
2
2
2
2
2
2
2
2
2
2
Fig. 2.5 Light source compartment
WARNING
HOT SURFACE
The light source and light source compartment both get very hot. To change a light source, turn off the power
and then change the light source only after checking to see that it has cooled sufficiently.
1
Deuterium Lamp (D2 Lamp) *

* For UV-Visible model only.
This is the ultraviolet range (190 nm to light source switch wavelength*) light source.
Tungsten Halogen Lamp (WI Lamp)

This is the visible and near infrared range (light source switch wavelength* to 1100 nm) light
source.
2
2
* Light source switch wavelength

The light source switch can be set anywhere in the range from 295 nm to 364 nm in 1 nm
increments.
For details, refer to Chapter 8, "8.2 Setting Instrument Parameters", <3. Light source>.
UVmini-1240
2-7
2.4 Light Source Compartment
2
2
2
2
2
2
2
2
2
2
2
2
2-8
UVmini-1240
3
3 Mode Selection and Shared Operations
Chapter 3 Mode Selection

and Shared Operations
3
3
3
3
CONTENTS
3.1
3.2
3.3
3.4
Mode Selection Screen ..................................................................................................................... 3-2

3.1.1 Set Parameters [Params]/[F1] .............................................................................................. 3-3
3.1.2 IC Card [IC Card]/[F2]........................................................................................................... 3-4
3.1.3 File Transfer [F3] .................................................................................................................. 3-4
3.1.4 External Control [F4]............................................................................................................. 3-4
Overview of Various Modes............................................................................................................... 3-5
Operations Shared by Modes ............................................................................................................ 3-7
3.3.2 Save Curve and Call Curve [SavCurve]/[CallCurv]............................................................... 3-8
Screen Copy and Data Printout ....................................................................................................... 3-13
3
3
3
3
3
3
UVmini-1240
3-1
3.1
3.1
Mode Selection Screen
After initialization is completed, the Mode Selection screen is displayed.
Indicates that this is the

Mode Selection screen.
Current wavelength and

photometric value.
Mode
3
3
3
3
3
Press when loading parameters
which have been stored in internal
memory or a data pack (optional).
3
3
Press when excuting Data/

program pack utilities.
Press when spectrophotometer is being

controlled by an external computer.
Press when transferring a data file to a PC
using the optional PC software.
Fig. 3.1 Mode Selection screen

Enter an item number 1. through 5. to select that mode.
When you select the respective modes, the Parameter Configuration screen shown in "3.2 Overview of Various
Modes" will be displayed.
3
3
3-2
UVmini-1240
3.1 Mode Selection Screen
3.1.1 Set Parameters [Params]/[F1]
In order to facilitate the entering of measurement parameters for each application, you can save the
measurement mode and measurement parameters in memory and then load them from the Mode
Selection screen. (Refer to "3.3.1 Save Parameters [SavParam]" about saving measurement
parameters.)
Up to 16 sets of measurement parameters can be stored in the instrument memory, and if a data pack
(optional) is used, a total of 62 parameter sets can be stored.
When you select [Params], the parameter file list will be displayed.
3
3
3
3
3
3
Set the file No. to be loaded.
Delete a file.
Fig. 3.2 Parameter file list
Select [Set] and enter the file No. for the parameters to be loaded. The screen will switch to the Parameters
Configuration screen for the measurement mode stored in that file.
You can delete the file corresponding to the specified number after selecting [Delete].
You can also use the
keys to change the page displayed in the Parameter file list screen.
3
3
UVmini-1240
3-3
3.1 Mode Selection Screen
3.1.2 IC Card [IC Card]/[F2]
This displays the IC card utilities screen.
3
3
3
Fig. 3.3 IC card utilities screen
There are 4 functions available for the optional IC cards (data pack and program pack).
<1. Data pack initialize>
This function is the equivalent of the format disk command on a personal computer.
A data pack absolutely must be initialized before it is used.
3
3
CAUTION
Initializing a data pack that is in use will delete all of the information stored on it.
<2. Data pack copy>

This function copies the contents of one data pack to another data pack. Please note that the
optional program pack can not be copied.
<3. Program pack check>
This is to check the contents of the program pack. The results of the title, mode No., version no.
and check sum (check for content errors) checks will be displayed.
<4. Option program area initialize>
This function is used for updating the version of the optional programs. The optional programs
loaded on the main unit will be deleted.
3
3
3.1.3 File Transfer [F3]

The screen will switch to the file transfer mode screen. This mode is to transfer the data files and
parameters files to an external computer. For details, see Chapter 10 "File Transfer".
3.1.4 External Control [F4]

The spectrophotometer can be controlled by an external computer.
For details, see Chapter 9 "PC Control".
3-4
UVmini-1240
3.2
3.2
Overview of Various Modes
When you select the various modes in the Mode Selection screen, their respective Parameters Configuration
screens will be displayed. The Parameter Configuration screen for each mode and an overview of each are
shown below.
Table 3.1 List of Parameter Configuration Screens

Item
Configuration Screen
Overview of Respective Mode
Measures the absorbance or %

transmittance of a sample at a
fixed wavelength.
1. Photometric
3
(See Chapter 4 "Photometric
Mode")
2. Spectrum
Scans a wavelength range to

measure the absorbance and %
transmittance of a sample as a
function of wavelength.
Energy measurement of a lamp
can also be performed.
3
3
3
(See Chapter 5 "Spectrum Mode")
3
3
3
3
3
UVmini-1240
3-5
3.2 Overview of Various Modes
Item
Configuration Screen
3. Quantitation
Creates a calibration curve from a

standard sample and quantitates
an unknown sample.
Measurement methods are
1-wavelength method,
2-wavelength method,
3-wavelength method.
In addition, the following methods
are available for calibration curve
generation.
K-factor method
Single point calibration curve
method
Multi-point calibration curve
method
(See Chapter 6 "Quantitation
Mode")
4. Optional
Program Pack
Select this when using the

optional program pack.
(See Chapter 7 "Optional
Program Pack")
3
3
3
3
Overview of Respective Mode
This is the mode for setting and

changing the basic operating
parameters of the instrument.
3
5. Utilities
3
3
(See Chapter 8 "Utilities Mode")
3-6
UVmini-1240
3.3
3.3
Operations Shared by Modes
Operations which are shared by each measurement mode will be explained here. The operations which are
unique to the various modes will be explained in the chapter on the respective mode.
3.3.1 Save Parameters [SavParam]

Photometric
Parameter Configuration screen
F4 key
Spectrum
F4 key
Quantitation
F4 key
This is the function for saving measurement parameters for the current measurement mode in the
instrument memory or a data pack (optional). The stored parameters can be loaded using [Params].
When you select [SavParam], a parameter file list is displayed.
3
3
3
3
3
3
Fig. 3.4 Parameter file list
NOTE
Note that the measurement parameter of accessories connected to the sample compartment is not saved.
When you select [Save]/ F2 key and enter the file No. in which the parameters are to be saved, the
Character input screen will be displayed.
UVmini-1240
3-7
3.3 Operations Shared by Modes
3
3
Fig. 3.5 Character input screen
Use the
keys to move the cursor to the character that you wish to enter and then press the
[ENTER] key to enter the character. The digits 0 through 9 can be directly entered by pressing the
corresponding number key. (Up to 8 characters)
If you make a mistake entering a character, you can delete it by pressing the [CE] key.
The space between the Z and 0 (zero) is a "space" which can also be entered. However, a "space"
can not be placed at the front of a file name.
3
3
When you finish the entry and select [End]/ F1 , the measurement parameters will be saved to the
file of the specified number under the file name which was entered.
3.3.2 Save Curve and Call Curve [SavCurve]/[CallCurv]
[SavCurve] is the function which saves spectrum data to a file.

Also, [CallCurv] is the function for loading to the screen curves which have been saved in a file.
Up to 6 curves can be saved in the instrument memory, while a total of 27 can be saved if an optional
data pack (Optional Accessory) is used.
Of these, the data measured in the immediately preceding measurement are automatically saved in
the dedicated File No. 0 (zero) under the file name "Original", and data can not be manually saved to
this file using [SavCurve].
3-8
UVmini-1240
When you select [SavCurve] or [CallCurv], a Curve file directory is displayed on the screen. However,
if there are no measurement data from an immediately preceding measurement, the [Save] function
will not be displayed.
3
The most recently acquired
data are automatically
saved in File No. 0 (zero)
under file name Original.
3
3
3
3
Specify a file No. to display
the data on screen.
Save data.
Copy data in one file to

a different file No.
Display a list of the

contents of each file.
Fig. 3.6 Curve file directory screen

The displayed page of the Curve file directory screen can be switched using the
keys.
[Load] F1 key;
This displays the data for the specified file No. on the screen.
If the data type (the vertical axis unit and measured mode are the same) and horizontal axis units
match, data can be overlaid on the same screen by consecutively entering file Nos.
When overlaying data, the vertical and horizontal axes for the initially displayed data will be displayed.
3
3
3
NOTE
Since the files which are displayed on the curve file screen or the data file screen use the same
area of memory, identical files will be displayed. Consequently, the data files for the various modes
are mixed together. Files of different modes can not be displayed on screens for measurement
mode.
UVmini-1240
3-9
[Save] F2 key;
This saves measured or processed data in memory areas No. 1 through 27.
When you press [Save], you will be instructed on the screen to enter a file No. When you enter a file
No. 1 through 27, the Character input screen will be displayed.
3
3
3
Fig. 3.7 Character input screen
Use the
keys to move the cursor to the character that you wish to enter and then press the
[ENTER] key to input the character. The digits 0 through 9 can be directly entered by pressing the
corresponding number key. (Up to 8 characters)
If you make a mistake entering a character, you can delete it by pressing the [CE] key.
The space between the Z and 0 (zero) is a "space" which can also be entered. However, a "space"
can not be placed at the front of a file name.
When you are finished entering and select [End] , the measurement parameters will be saved to the
file of the specified number under the file name which was entered.
3
3
3
[Copy] F3 key;
You can copy data between file Nos. 0 through 27. Specify the copy source and copy destination file
Nos. as instructed on the screen. No. 0 can't be specified as the destination.
[Header] F4 key;
You can display the range of the horizontal axis (wavelength range) and the units thereof (nm) as well
as the units of the vertical axis (ABS, T%, E) in the list (Curve file list) so as to see the type of data
saved in each of the file Nos. 0 through 27.
3
3
3-10
UVmini-1240
3
Range of horizontal axis
Unit of horizontal axis *
Measurement mode (unit)
3
3
Fig. 3.8 Curve file list screen (Header)

* The data for Photometrics or Quantitation will display the measured wavelength.
File No. 0 "Original";
The most recently acquired data are automatically saved in the dedicated File No. 0 (zero) under the
file name "Original".
The screen can be returned to the immediately preceding condition after file processing, such as
display or copy, have been performed by loading file No. 0.
Other data can not be saved in file No. 0.
3
3
3
3.3.3 Data Management [DataFile]
Photometric
Measurement screen
F2 key
Quantitation
Measure Unknown Sample screen
F2 key
3
3
Numeric data tables, such as the photometric values and concentrations obtained in the Photometric
and Quantitation modes, can be saved to and loaded from the instrument memory or an data pack
(optional).
Up to 6 files of data tables can be saved in the instrument memory, while if an data pack (optional) is
also used, a total of 27 files can be saved.
In addition, the maximum quantity of data comprising the various data tables is 227 items of data for
Photometric mode and 185 items of data for Quantitation mode.
Of these, the table data up to the immediately preceding measurement are automatically saved in the
dedicated File No. 0 (zero) under the file name "Original".
UVmini-1240
3
3
3
3-11
The Data file list screen is displayed when you select [DataFile].
The most recently

acquired data are
automatically saved File
No. 0 (zero) under file
name Original.
3
3
Load a data table to the

screen.
Save the current

measurement data table.
Copy data in one file No. to a

different file No.
Display a list of the

contents of each file.
3
Fig. 3.9 Data File List screen
The operation of each function is the same as [SavCurve] and [CallCurv].

Now, if there are no measurement data from the immediately preceding measurement, the [Save]
function is not displayed.
3
3
3
3
3
3-12
UVmini-1240
3.4
3.4
Screen Copy and Data Printout
When an ESC/P printer, a PCL3 printer, or thermal printer HCP (DPU) is installed, the UVmini-1240/V
can print out hard copies of the screen or the measurement results. Connecting this printer makes the
following three types of printouts possible.
1
Hard copy of screen (Fig. 3.10)

Press the [PRINT] key to print a hard copy of the screen. Printing of date, function display, and
printing area can be selected.
Print using function keys

When a function key has a [Print Out] function, as in a mode such as Photometrics in which the
measurement results are recorded in table form, all of the tabular data will be printed out.
Print numeric values for each data

In modes in which the measurement results are obtained as numeric values, as each
measurement is acquired (as with Photometric), these measurements will automatically be
printed.
3
3
The UVmini-1240/V can be connected to a commercially available printer for personal computer
that conforms to EPSON's ESC/P printer command language. Connecting to an ESC/P printer
enables the following output, in addition to the above mentioned three types of printouts. Both
ESC/P-24 and the ESC/P-9 are compatible.
4
3
3
Print waveform
In the spectrum mode, it is possible to plot the waveform (spectrum) on A4 size paper when it is
displayed on the measurement screen. It is half as large as A4 paper. Examples of each mode by
a 24-pin printer are displayed in Fig. 3.11.
By pressing the [PRINT] key while the waveform is being displayed, printing of the waveform or a
hard copy of it can be selected.
3
3
For the connection of the printer, refer to "Appendix A-2 Printer".
3
3
3
3
3
UVmini-1240
3-13
3.4 Screen Copy and Data Printout
3
3
Fig. 3.10 Hard copy of screen
3
3
3
3
3
3
3
3
Fig. 3.11 Waveform print
3-14
UVmini-1240
4
4 Photometric Mode
Chapter 4 Photometric
Mode
4
4
4
This is the fixed wavelength measurement mode. This measures the absorbance (ABS) or % transmittance
(T%) at a fixed wavelength.
By repeating a measurement, you can create and display a table of the measurement results.
It is also possible to quantitate the results by the K-factor method.
This is a simple quantitation method. A sample concentration C is expressed as C=K*ABS, and when the value
for K (K = Conc. of Std / absorbance of Std) is already known, you can enter the value for K and measure the
concentration of unknown samples.
Up to 227 items of data can be saved in one file.
4
4
4
CONTENTS
4.1
4.2

4.1.1 Set Wavelength .................................................................................................................... 4-2
4.1.2 [T%/ABS] .............................................................................................................................. 4-2
4.1.6 Auto zeroing [AUTO ZERO].................................................................................................. 4-4
4.1.7 Change Sample No. [Smpl No.]............................................................................................ 4-4
4.1.8 Quantitation by K-factor method [Factor K] .......................................................................... 4-4
4.2.1 Data Print and Screen Copy ................................................................................................. 4-5
4.2.2 Data Management [DataFile]................................................................................................ 4-5
4.2.3 Data Display [DataDisp]........................................................................................................ 4-5
UVmini-1240
4
4
4
4-1
4.1
4.1
Measurement Parameter Configuration Screen
When you select <1. Photometric> in the Mode Selection screen, the measurement parameter configuration
screen will be displayed.
Indicates that this is the

Photometric mode.
4
Indicates current
wavelength.
Current photometric value.
4
4
4
Each time you press this, the

display switches between T%
(% transmittance) and ABS
(absorbance).
Determines the sample module

control parameters. When you press
this function, the screen switches to
the Sample Control screen.
Switch to the
Measurement
screen.
Press this to save the

current measurement
parameters.
Fig. 4.1 Measurement parameter configuration screen
4.1.1 Set Wavelength

Use the [GOTO WL] key to set the wavelength.
4.1.2 [T%/ABS]
You can switch between the % transmittance mode (T%) and absorbance mode (ABS) each time you
press the [T%/ABS] key.
4.1.3 Sample Compartment Control [SmplCmpt]

4
Performs settings of the sample compartment.

The setting items include types of the sample compartment, number of cells, and operation condition
of the sipper.
For the details, see Appendix A-1 "Sample Module (Multi-cell, Sipper Operation)".

You can save the current measurement parameters to internal memory or to a data pack (optional).
(See 3.3.1 "Save Parameters [SavParam]")
4-2
UVmini-1240
4.1 Measurement Parameter Configuration Screen
4.1.5 Measurement Screen [SmplMeas]
Determine the parameters and then press the [SmplMeas] or [START/STOP] key to switch to then
measurement screen.
(At the same time that you press the [START/STOP] key, the first measurement will be performed.)
The method of assigning sample Nos. in the measurement screen in Fig. 4.2(A) differs when a
standard sample compartment, Sipper 160 and syringe sipper (both options) are used, and when a
multi-cell and CPS-240 (all options) are used.
Sample No.
Measured value
Current wavelength and

photometric value
4
Measured value
multiplied by K-factor
Measurement results
are displayed here.
4
4
4
Current factor value.
Press to change to
the next sample No.
Enables you to save or

recall measurement data.
Function for displaying

a data table.
Enter the factor for

the K-factor method.
4
Fig. 4.2(A) Photometric measurement screen
(for standard cell holder or when only one cell is being used)
4
4
4
4
UVmini-1240
4-3
Cell No.
Measurement repetitions
4
4
Fig. 4.2(B) Photometric measurement screen

(when up to 6 cells are occupied when using a multi-cell or CPS-240A)
4.1.6 Auto zeroing [AUTO ZERO]

When blank correction is necessary, set the blank sample and press the [AUTO ZERO] key before
measurement. The photometric value at this time will be set to 0ABS (100%).
4
4
4.1.7 Change Sample No. [Smpl No.]

You can reassign the number for the next sample being measured by pressing [Smpl No.] (F1) in the
measurement screen.
The sample number input range is 0 to 9999.
4
4
4.1.8 Quantitation by K-factor method [Factor K]

When the absorbance and concentration are directly proportional, this is a simple quantitation method
that calculates the concentration by multiplying the absorbance by a certain conversion factor K.
In the measurement screen, select [Factor K] and then enter the value for K from the keyboard as
instructed on the screen.
The current value for K is displayed in the lower-right of the screen. In addition, the value for factor
* absorbance =K * ABS is displayed in the right-hand column of the table.
In the transmittance mode, the value for factor K*T% is displayed in the right-hand column of the table.
NOTE
When performing quantitation by the K-factor method, it is suggested that the measurement mode
be set to ABS (absorbance).
4-4
UVmini-1240
4.2
4.2
Post-measurement Processing
4.2.1 Data Print and Screen Copy
When a printer (optional) is connected and the automatic print setting of the utilities is turned on, the
measurement results will be printed on the printer for every measurement. At the same time, the data
will be output to the RS-232C port.
4
4
Fig. 4.3 Sample printout
You can also print a hard copy of the current screen on the printer by pressing the [PRINT] key.

This function is for saving the measurement data to memory, or calling up data to the screen.
(See 3.3.3 "Data Management [DataFile]")
4.2.3 Data Display [DataDisp]
This function is for displaying a list of measurement data including the ones that have disappeared
from the screen. (When measurement is repeated, the measurement data are listed in a scrolling list
that displays up to the last seven measurements. If there are more than seven items, the previous
items are not displayed.)
Using this function, it is also possible to print data list table or to delete data.
4
4
4
4
UVmini-1240
4-5
4.2 Post-measurement Processing
Data are displayed from

No. 1.
4
4
Use this to delete data.
Use this to print data as numeric data.
Fig. 4.4 Data Display screen (Photometric mode)
Using the
or
key, eight items of data can be scrolled at one time.
[Delete] F1 key;
This enables you to delete the entire numeric data table at once.
[PrintOut] F2 key;
This enables you to print all of the data as a numeric data table on the printer (optional). (The [PRINT]
key on the keyboard will provide a hard copy of the screen currently being displayed.)
4
4
4
4
4
Fig. 4.5 Printer output (Photometric mode)
4-6
UVmini-1240
On this occasion, the same data is output to the RS-232C port. The output format of the data is the
same as the print output example shown in Fig. 4.5. At the end of each line, [CR](0Dh) and [LF] (0Ah)
codes are output. At the end of each data, the [SB](1Ah) code is displayed.
The transfer specifications of the RS-232C are as follows.
Baud rate:
9600 bps
Data bit length: 7
Stop bit:
1
Parity:
Odd number (odd)
X parameter: None
4
For details about the connection cable, see Chapter 9, 9.1 "Connecting to a PC".
NOTE
The external transmission function comprises one-direction transmission of data from the UVmini1240.
Data transmission is executed continuously in one direction at a speed which presumes that the
data are being loaded to a PC. Data are output from the RS-232C port approximately every 20ms.
While this speed will be appropriate for most PCs, data may be lost with a slower PC. In addition, if
you connect a printer to the RS-232C interface and attempt to print the transmission data, all of the
data may not be able to be printed if the printer has a small internal buffer.
4
4
4
4
4
4
4
4
4
4
UVmini-1240
4-7
4
4
4
4
4
4
4
4
4-8
UVmini-1240
5
5 Spectrum Mode
Chapter 5 Spectrum Mode
5
5
This is the mode in which spectral measurement is performed.
There are three types of measurement available: ABS (absorbance), T% (% transmittance) and single beam E
(energy).
Measured spectra can be saved to memory and be subjected to various data processing such as changing the
display scale and peak detection.
5
5
CONTENTS
5.1
5.2
5.3
5.4

5.1.1 Setting Parameter Items ....................................................................................................... 5-3
5.1.2 Baseline Correction [BaseCorr] ............................................................................................ 5-5
5.1.3 Call Curve [CallCurv] ............................................................................................................ 5-5
Measurement..................................................................................................................................... 5-6
5.3.1 Read Cursor ......................................................................................................................... 5-7
5.3.2 Enlarge/Reduce [Zoom]........................................................................................................ 5-7
5.3.3 Peak detection ...................................................................................................................... 5-9
5.3.4 External Transmission [ExtTrans]....................................................................................... 5-10
5.3.5 Save Curve [SavCurve] ...................................................................................................... 5-11
5.3.6 Waveform printing............................................................................................................... 5-11
Scanning Speed and Data Sampling Interval .................................................................................. 5-12
UVmini-1240
5
5
5
5
5-1
5.1
5.1
When you select <2. Spectrum> in the Mode Selection screen, the measurement parameter
configuration screen will be displayed.
The setting items of measurement parameter configuration screens differ slightly for the ABS
(absorbance) and T% (% transmittance) measurement modes (Fig. 5.1) and for the E (energy)
measurement mode (Fig. 5.2).
Shows that the instrument is in

spectrum mode.
Select absorbance
(ABS), % transmittance
(T%) or energy (E)
measurement.
Shows the current wavelength

and photometric value.
Shows the status of

light source.
Shows the wavelength

range over which the
spectrum will be
measured.
Shows how many

times the spectrum
will be measured for
the same sample
each time the
[START] key is
pressed.
Shows the vertical axis

range when a spectrum
is displayed.
Shows the scan

speed.
Select whether to
overlay each spectrum
on the same screen as
they are measured or to
renew the screen,
deleting the previously
acquired data each time
a spectrum is
measured.
Perform baseline
correction.
5
5
5
Indicates the
operating mode.
Call up data stored

in internal memory
or a data pack.
Set parameters for

the sample module.
Enables current
measurement
parameters to be saved.
Fig. 5.1 Measurement parameter configuration screen (ABS, T% modes)
5
5
5
5
Set the gain for the
photometer amplifier.
Set the light source to

be used.
Fig. 5.2 Measurement parameter configuration screen (E mode)
5-2
UVmini-1240
5.1.1 Setting Parameter Items
Set the measurement parameter for each item using key dialogue.
<1. Meas. mode>
Successive selection of item number 1 cycles from ABS T% E . Simultaneously, the range in
<3. Rec. range> also cycles from ABS, T% to E.
<2. range>
Set the range of the wavelength scan. Enter the scan start and end wavelength, in that order.
The wavelength input range is 190 nm to 1100 nm and the starting wavelength must be the
longer wavelength of the two.
The minimum scan range is 1 nm.
(Scan start wavelength) - (Scan end wavelength) >= 1 nm
The above-mentioned wavelength range can be set for the visible spectrophotometer model.
Effective measuring range: 325 nm to 1100 nm
<3. Rec. range>

Set the range for the vertical axis during spectrum recording. Input ranges
ABS: 3.99A
T%: 399%
E: 399E
5
5
<4. Scan speed>

The following 5 wavelength scanning speed levels are available.
1. Very Fast
2. Fast
3. Medium
4. Slow
5. Very Slow
Selecting the scan speed fundamentally means selecting the data sampling interval. The faster
the speed, the wider the sampling interval becomes making the data points less. As a result, a
shorter time is required for scanning.
However, when the scan range is set wide, due to limited memory capacity to store data (within
1,000 data points), the sampling interval becomes wide even when selecting low speed, meaning
that the scan speed does not vary much from that used when high speed is selected.
(See 5.4 "Scanning Speed and Data Sampling Interval")
5
5
5
5
5
5
5
UVmini-1240
5-3
<5. No. of scans>

Set the number of times a scan will be repeated.
If this is set to 2 or more times, the interval setting will be displayed with the scanning repetitions
on the same display line.
The scan interval will be displayed

when the number of repetitions is
set to 2 or more.
5
Fig. 5.3 Parameter Configuration screen with scan interval displayed
When you press the [START/STOP] key once, measurement will be repeated only the set
number of times. The scan interval is the time from the scan start time to the next start time.
If the time required for the actual scan is longer than the set time for the scan interval, the next
scan will be performed without any wait time.
On this occasion, the cycle of the parameter is changed to the time actually required.
If the set scan interval time is longer than the time required for the actual scan, the next scan will
start after waiting the amount of time equal to the difference between the two times. During the
interval, the number of remaining scan repetitions and the remaining wait time will be displayed.
5
5
5
<6. Display mode>

This parameter will toggle between Sequential<->Overlay each time this item is selected.
Sequential; The screen is renewed for each scan and only the spectrum from that measurement
is displayed.
Overlay;
The spectrum display for each scan is left as it is so that multiple spectra are overlaid
in the display. If the No. of scans is set to 2 or more, each spectrum will be overlaid
on the preceding spectra.
5
5
5
<7. Gain> (E mode only)

This will be displayed only in the E (energy) mode.
This sets the gain for the photometer amplifier so that measurement can be performed without
saturation of the energy curve.
The minimum setting is 1 and the maximum is 6. Sensitivity is increased by approximately 4
times with each level.
5-4
UVmini-1240
<8. Light Source> (E mode only)

This will be displayed only in the E (energy) mode
Select the light source when performing energy measurement. Measurement will be performed
using the light source selected here, regardless of the setting of light source selected in the
utilities mode.
WI lamp: The light source is set to the tungsten iodine lamp.
D2 lamp: The light source is set to the deuterium lamp.
OFF:
Turns OFF both the WI and D2 lamps. The light source mirror position is not determined.
5.1.2 Baseline Correction [BaseCorr]
This allows you to set a blank sample and correct the baseline under the measurement parameters
which have been set.
To abort correction, press the [START/STOP] key.
As the baseline is not stabilized just after initialization of the spectrophotometer, perform baseline
correction more than 10 minutes after the initialization of the spectrophotometer.
5.1.3 Call Curve [CallCurv]

5
This allows you to recall data which have been stored in the instrument memory or a data pack
(optional) to the screen.
(See 3.3.2 "Save Curve and Call Curve [SavCurve]/[CallCurv]")

5
Perform the settings for the sample compartment.

The setting items include type of the sample compartment, number of cells, and operation condition of
the sipper.
For the details, see Appendix A-1 "Sample Module (Multi-cell, Sipper Operation)".
You can save the current measurement parameters to the instrument memory or a data pack card
(option).
5
5
5
UVmini-1240
5-5
5.2
5.2
Measurement
Once you have determined the parameters and press the [START/STOP] key, the display will switch
to the measurement screen and measurement will begin. The measurement screen at the end of
measurement be similar to that shown in Fig. 5.4.
5
5
Enables you to change the
ranges on the vertical and
horizontal axes.
5
5
Detects and displays

peaks and valleys.
Transmits the data to an

external computer, etc.
Enables you to save the

spectrum to memory.
Fig. 5.4 Measurement screen

Press the [START/STOP] key again to interrupt measurement.
The measurement results can be output to a printer (optional) as a hard copy of the screen by
pressing the [PRINT] key. When connected to an ESC/P printer, it is possible not only to print a hard
copy but also to plot the spectrum at a size that is half of A4-size paper. (Waveform printing)
Moreover, when the automatic print setting of utilities is turned on, the screen copy is automatically
printed after the spectrum scanning.
Pressing the [RETURN] key returns the display to the measurement parameter configuration screen.
On this occasion, in order to display the measurement data again, specify "0: Original" by [CallCurv]
on the parameter configuration screen.
However, please note that only the data measured last time is displayed where the overlaying
measurement is being performed, even though the above operation is performed.
5
5
5
5
5
5-6
UVmini-1240
5.3
5.3
Post-measurement Processing
After measurement is completed, you can perform the following types of data processing.
5.3.1 Read Cursor

You can read the photometric value at any wavelength on the spectrum using the cursor.
Cursor position
Photometric value read
When the cursor is

displayed, this frame is
highlighted.
5
Cursor
5
5
5
Fig. 5.5 Read cursor screen
In a screen in which a spectrum is being displayed, the cursor will be displayed when you press a
cursor key (
). Hold the cursor key down to move the cursor quickly. At the same time, the
frame at the top-right of the screen which normally displays the current wavelength and data will be
highlighted and the wavelength and photometric value at the cursor position can be read.
Press any key except for a cursor key
and the [PRINT] key to make the cursor disappear.
When the Rec. mode is set to Overlay, the wavelength and photometric value at the cursor position
will be displayed for the most recently displayed curve.
5
5
5
5.3.2 Enlarge/Reduce [Zoom]

5
You can enlarge and reduce the spectrum by changing the ranges of the vertical and horizontal axes
of the displayed spectrum.
(Enlargement is available for the horizontal axis only.)
5
Select [Zoom] and enter the values for the vertical axis maximum and minimum and horizontal axis
minimum and maximum, in that order, to change the ranges on the vertical and horizontal axes.
If you press the
keys while entering horizontal axis values, the cursor will be displayed,
enabling data values to be read directly from the curve. Press [Enter] while the cursor is displayed and
the value of the point on the horizontal axis on which the cursor is resting at that time will become the
entered value.
UVmini-1240
5-7
Spectrum screen
Select [Zoom]
(A)
Zoom
Example where the

0~1Abs range on
vertical axis and the
400~550 nm range on
the horizontal axis in
(A) is zoomed.
5
(B)
5
5
Saves the data in (B).
5
5
Restores the display to the original data (A).
(A) Spectrum screen

(B) Zoomed screen
Fig. 5.6 Zoom function
Select [Restore] to return the zoomed data to the original data.

Even if you execute [Zoom] again, [Restore] will return to the (A) display.
You can save the data resulting from the [Zoom] function by selecting [SavCurve] in the (B) display
(see 3.3.2 "Save Curve and Call Curve [SavCurve]/[CallCurv]").
5-8
UVmini-1240
5
NOTE
1) If curves are overlaid on the screen using the [CallCurv] function, a [Zoom] will be executed only on the
data which were displayed on the screen first, and the other data will be deleted from the screen.
2) When measurement results have been overlaid, a [Zoom] command will be executed on the most
recently acquired data; all other data will be deleted.
5.3.3 Peak detection

This detects the peaks or valleys in the curve data and displays a list of the results. When selecting
[Peak] or [Valley], the peak table shown in Fig. 5.7 is changed over. Up to 20 peaks or valleys can be
detected.
(Peak table)
5
5
5
5
5
Fig. 5.7 Peak detection (Peak table)
5
Peak/valley mark
(Peak/valley marks)
5
5
Fig. 5.8 Peak detection screen

When selecting [Graph] in the Peak detection screen, the curve data with the mark indicating peaks
and valleys will be displayed. When you press the [RETURN] key on the Peak detection screen, the
data before peak detection (without mark) will be displayed.
UVmini-1240
5-9
5.3.4 External Transmission [ExtTrans]
The spectrum data can be transferred to external devices such as computer via RS-232C port. This is
used when saving data on a PC and the like.
The data is output from the long wavelength side with the wavelength and its measurement values
arranged in pairs.
The data format is as follows.
Absorption:
ZZZZ.Z SX.XXXY[CR][LF]
Other than absorption: ZZZZ.Z SXXX.XY[CR][LF]
Z is wavelength, X and Y are measurement values, and is a space code. S indicates the codes of a
measurement value. When it is negative, "-" is output and when it is positive or 0, a space code is
output. Y is output only when the number of data display digit is set to Abs (4) T%(2) in the Utilities
mode. Either of [CR](0Dh) or [LF](0Ah) is output for each data pair.
[SB](1Ah) is output at the end of each data.
Baud rate:
Data bit length:
Stop bit:
Parity:
X parameter:
5
5
5
9600 bps
7
1
Odd number (odd)
None
For information about the connection cable, see Chapter 9, 9.1 "Connecting to a PC".
NOTE
The external transmission function comprises one-direction transmission of data from the UVmini1240.
5
5
5
5
5
5-10
UVmini-1240
5.3.5 Save Curve [SavCurve]
This enables you to save the spectrum data to the instrument memory or a data pack (optional).
(See 3.3.2 "Save Curve and Call Curve [SavCurve]/[CallCurv]")
5.3.6 Waveform printing

Waveform printing functions when printer selection is set to the ESC/P-24 or the ESC/P-9, and the
printer compatible to the printer command ESC/P is connected.
When spectrum is displayed on the measurement screen, its waveform can be plotted on A4 size
paper.
The size is half of the A4 size paper. An output example is shown in Fig. 3.11. The example was
output by a 24-pin printer. Waveform printing (hard copy) can be selected by pressing the [PRINT] key
while the waveform is being displayed.
Select waveform printing using the
or
key and press the [ENTER] key.
The grid for the output data can be selected among the following four grids. Select the one you desire
using the
or
key and press the [ENTER] key.
The waveform to be printed by waveform printing is either the data of the latest measurement or the
data that has been initially called by Call Curve (Save Curve.) When more than one waveform is
displayed with overlapping, the one which has been measured last will be printed.
5
5
5
5
5
5
5
5
5
UVmini-1240
5-11
5.4
5.4
Scanning Speed and Data Sampling Interval
The data sampling interval is determined by the set scanning speed and the scanning wavelength
range.
According to these two setting conditions, the data sampling interval is determined as shown in Table
5.1.
Table 5.1 Data Sampling Interval

Scan range
(Start wavelength to end wavelength) [mm]
Less than 100
nm
Less than 200

nm
Less than 500

nm
500 nm or more
0.1
0.2
0.5
1.0
0.5
1.0
Very Slow
Scan speed
Slow
0.2
Medium
0.5
1.0
Fast
1.0
Very Fast
2.0
The wider the data sampling interval is, the less the obtained data points become, which effectively
makes the scan speed faster.
Another condition relating to the scan speed is the value for the data accumulation time set in the
Utilities mode of the main unit. The longer the data accumulation time is set, the noise becomes less
in the data to be obtained. However, this requires more time to obtain data and as a result, scan speed
decreases. The scan speed becomes as follows according to the set values of the data sampling
interval and the data accumulation time. (Table 5.2)
5
5
5
Table 5.2 Scan speed [nm/min.]

Set values of the data accumulation time [sec]
5
Data sampling
interval [nm]
5
5
5
0.05
0.1
0.2
2.0
1400
800
480
1.0
700
400
240
0.5
350
200
120
0.2
140
80
48
0.1
70
40
24
5
NOTE
When setting of the data accumulation time is changed, baseline correction must be performed
again.
5-12
UVmini-1240
6
6 Quantitation Mode
Chapter 6 Quantitation
Mode
CONTENTS
6.1
6.2
6.3
6.4

6.1.1 Measurement Parameters .................................................................................................... 6-3
Creating a Calibration Curve ............................................................................................................. 6-9
6.2.1 Inputting Concentration......................................................................................................... 6-9
6.2.2 Entering the Absorbance for a Single Point Calibration Curve ............................................. 6-9
6.2.3 Entering the Absorbance for a Multi-point Calibration Curve.............................................. 6-10
6.2.4 Concentration Table ........................................................................................................... 6-11
6.2.5 Calibration Curve Display ................................................................................................... 6-12
6.2.6 Changing the Concentration Table ..................................................................................... 6-13
Unknown Sample Measurement (Quantitation)............................................................................... 6-14
6.3.1 Measurement Screen ......................................................................................................... 6-14
6.3.2 Changing the Sample Number ........................................................................................... 6-16
6.3.4 Data Display [DataDisp]...................................................................................................... 6-16
6.3.5 Display Equation [Equation]................................................................................................ 6-19
Two/Three-wavelength Calibration method ..................................................................................... 6-20
6.4.1 Two-wavelength Quantitation ............................................................................................. 6-20
6.4.2 Three-wavelength Quantitation........................................................................................... 6-20
UVmini-1240
6
6
6
6
6
6
6-1
6.1
Quantitation Mode
This is the mode in which unknown samples are quantified by creating a calibration curve from
standard samples. The following three types of quantitation are available depending on the number of
wavelengths used in the measurement method:
6
6
One-wavelength method
Two-wavelength method
Three-wavelength method
The two/three-wavelength methods create the calibration curve and measure the unknown sample
using several wavelengths. These are effective in eliminating the effects of dispersion due to
interfering components and contaminants and in correcting "floating" of the baseline due to bubbles.
For more details, see 6.4 "Two/Three-wavelength Calibration method".
The following three methods are available for creating calibration curves.
6
6
K-factor (C=K*ABS+B) method

Single point calibration curve method
Multi-point calibration curve method
The K-factor method is one in which you manually enter the factors K and B for the calibration curve.
The Single point method is one in which the calibration curve is created by measuring a single
standard sample.
The Multi-point method is one in which the calibration curve is created by measuring multiple (up to
10) standard samples. The multi-point method will not only create a straight line, but can also
generate 2nd and 3rd order curve fits of the data.
The quantified data can be saved to memory or a data pack (optional). Up to 185 data can be saved
per file.
6
6
6
6
6
6
6-2
UVmini-1240
6.1
6.1
When you select <3. Quantitation> in the mode selection screen, the measurement parameters
configuration screen in Fig. 6.1 will be displayed.
Shows that the
instrument is in the
quantitation mode.
Shows the current

wavelength and
photometric value.
Shows which of the three

measurement methods is
being used.
Shows which of the three

Calibration methods is
being used.
Shows the calibration

curve factors, the
concentration/
absorbance of the
standard sample, or the
number of standard
samples. The data
displayed changes
depending on the
Calibration method used.
Shows the wavelength

being measured.
Shows the number of
times a measurement will
be repeated.
Shows the concentration
units of the samples.
Shows whether or not the

data will be printed for
each measurement.
Shows if measurement is
possible (calibration
curve has been created)
or if a calibration curve
must be created.
6
Set the sample module
parameters.
Switch to the
measurement screen.
Save measurement parameters,

including the calibration curve equation.
Fig. 6.1 Measurement parameter configuration screen
6.1.1 Measurement Parameters

Set the various measurement parameter items in a dialogue format. Select the parameter to be set by
entering the item number.
<1. Meas.> (Measurement method)
The measurement method selection screen is shown in Fig. 6.2.
6
6
6
6
6
Fig. 6.2 Measurement method selection screen

Select the measurement method from the following three types and then enter the
measurement wavelength as instructed on the screen.
1
Enter measurement wavelength 1.
UVmini-1240
6-3
2
Enter measurement wavelengths 1 and 2. 1 and 2 will be set in the order they were
entered.
(See 6.4 "Two/Three-wavelength Calibration method")
3
Enter measurement wavelengths 1, 2 and 3. 1, 2 and 3 will be sorted automatically in
the order of 1 > 2 > 3 after they are entered.
(See 6.4 "Two/Three-wavelength Calibration method")
<2. Method> (Calibration method)... (Create calibration curve)

The Calibration method can be selected from one of three methods, as shown in the screen in
Fig. 6.3. If you select single point calibration curve or multi-point calibration curve, create the
calibration curve before measuring the unknown sample.
6
6
6
Fig. 6.3 Calibration method selection screen
K-factor (C=K*ABS+B)
The relationship between the concentration C and absorbance ABS of a sample is expressed
as C=K*ABS+B, and when the values for the constants K and B are already known, you can
manually enter the values for K and B to create the calibration curve.
If you enter K and B, you can set a blank sample, press the AUTO ZERO key to set ABS=0
and immediately measure the blank sample.
6
6
6
6
Fig. 6.4 K/B input screen
6-4
UVmini-1240
Single point calib.

This will measure the concentration of an unknown sample by finding the value for K in the
calibration curve equation C=K*ABS from a single standard sample of known concentration.
The calibration curve will be a straight line defined by the origin and the absorbance and
concentration of the standard sample.Selecting single point calibration turns the parameter
configuration screen to the one shown in Fig. 6.5. Press the [START/STOP] key to enter the
calibration curve creation screen (Concentration table).
6
Fig. 6.5 Parameter configuration screen (Single point calibration curve)
Multi-point calib.
1st/2nd/3rd order calibration curves can be created by the least square method from 1 to 10
standard samples of known concentrations.
Enter the number of standards samples (1-10), the order number (1-3) and whether or not to
pass through the origin according to the instructions on the screen.
The number of standard samples must be more than the order number of the calibration curve
when "0 intercept: YES" is selected, and must be more than (order number + 1) when "0
intercept: NO" is selected.
6
6
6
6
6
6
Fig. 6.6 Multi-point calibration curve parameter configuration screen

When setting of the items is completed, the screen will return to the parameter configuration
screen. (Fig. 6.7) If you press the START/STOP key at this time, the display will switch to the
calibration curve creation screen (concentration table). (Fig. 6.12)
UVmini-1240
6-5
Fig. 6.7 Parameter configuration screen (Multi-point calibration curve)

<3. No. of Meas.> (Number of Measurements)
Determine how many times the same sample will be measured during measurement.
If this is set to two or more, the average value of the multiple measurements will be used as the
ABS value for the standard sample when the calibration curve is created. When measuring an
unknown sample, the measured value for each repetition and the average value will be
displayed.
(See 6.3.1 "Measurement Screen")
6
6
NOTE
If you interrupt repetitive measurements by pressing the RETURN key, the average value will not
be calculated.
6
6
Repetitions = 3
6
Average value
6
6
Fig. 6.8 Sample data display screen (Unknown sample measurement screen)
NOTE
When a multi-cell or CPS-240 (all options) is used and the number of cells has been set to two or
more, the No. of Meas. function takes precedence. In this case, automatic measurement using
multiple cells cannot be performed.
6-6
UVmini-1240
<4. Unit>
Specify the unit of concentration using number 0 through 9.
Also, you can register and use an arbitrary unit (its name can be set using up to 6 characters).
Fig. 6.9 Unit input screen
6
6
6
6
6
6
Fig. 6.10 Unit registration screen
Use the
,
, H , G keys to move the cursor to the character that you wish to enter
and then press the ENTER key to input the character. The characters 0 through 9 can be
directly entered by pressing the corresponding number key. (Up to 6 characters)
If you make a mistake entering a character, you can delete it with the CE key.
The space between the Z and 0 (zero) is a "space" which can also be entered.
When you are finished entering and select End , the unit is registered.
UVmini-1240
6-7
<5. Data print>

You can select whether or not to print the data for each measurement to the printer (optional).
Fig. 6.11 Sample printout

When data print is set to "YES", the data for each unknown sample measurement will be
printed to the printer.
<6. Baseline correction>

This screen is displayed when two-or three-wavelength quantitation is selected.
Set a blank sample and perform baseline correction in the specified wavelength range. To
cancel correction, press the START/STOP key.
6
6
Set the parameters such as the sample module being used, the number of cells, etc.
Perform the settings for the sample compartment.
The setting items include type of the sample compartment, number of cells, and operation condition of
the sipper.
For details, see A-1 "Sample Module (Multi-cell, Sipper Operation)".
6
6
6.1.3 Measurement Screen [SmplMeas]

6
Switch to the screen for unknown sample measurement.


This enables you to save the current measurement parameters, including the calibration curve which
has been created, to the instrument memory or a data pack (optional).
6-8
UVmini-1240
6.2
6.2
Creating a Calibration Curve
If single point calibration curve or multi-point calibration curve is selected for the Calibration method, a
calibration curve must be created before performing measurement (quantitation) of the unknown
sample.
When the START/STOP key is pressed on the parameter configuration screen, the concentration
table is displayed on the screen and creation of a calibration curve starts. (Fig. 6.12)
6.2.1 Inputting Concentration

First, enter the concentration of the standard sample (STD). When inputting the concentration of more
than one standard sample, the order of inputting the values is irrelevant to level of concentration.
6
6
Fig. 6.12 Concentration table (Concentration input)
After entering the concentration levels of all the standard samples, determine the absorbance (ABS
value) of the standard samples. The method for entering the absorbance is different for single point
calibration curve and for multi-point calibration curve. For a multi-point calibration curve, sequential
measurement input is made possible by the multi-cell.
6
6
6.2.2 Entering the Absorbance for a Single Point Calibration Curve

The following two kinds of methods are provided for inputting the ABS value.
1)
Key input
The numerical value of ABS is input from the keyboard.
2)
Measurement input (only Cell 1 is used.)

Set the standard samples and press the START/STOP key.
In the case of multi-cell or the CPS-240 (all options), only Cell 1 is used.
6
6
6
UVmini-1240
6-9
6.2 Creating a Calibration Curve
6.2.3 Entering the Absorbance for a Multi-point Calibration Curve
The following three kinds of input methods are provided.

1)
Key input
The numerical value of absorbance is input from the keyboard.
2)
Measurement input (Only Cell 1 is used.)

Set the standard samples in Cell 1 of the standard sample compartment or the multi-cell in due
order. Start measurement by pressing the START/STOP key.
3)
Multi-cell sequential measurement input

Set the standard samples in the multi-cell, micro multi-cell, or CPS-240 from Cell 1 in due order.
Start a sequential measurement by pressing the START/STOP key.
If the number of the standard samples is larger than that of the accessory cells, measurement is
possible by replacing the cells in the middle. For example, if measurement is performed with eight
standard samples using a 6-position multi-cell, measurement is performed for up to 6 cells by pressing
the START/STOP key. To measure the remaining two cells, replace the cell (filling the cells from the
one with the smaller number), press the START/STOP key again.
6
6
6
6
6
Fig. 6.13 Multi-point calibration curve creation screen

If the number of repetitions is set to two or higher in the parameter configuration screen, an indicator
of the number of repetitions will be displayed and the average value will be used to determine the K
value.
The results of each measurement will be displayed in the measurement value table on the right. After
the repetitive measurements are completed, the average value (represented by m at the bottom of the
right side) will be displayed in the concentration table on the left.
6
6
6
6-10
UVmini-1240
Repetitive measurement
(3 times)
Average value of 3
repetitions
Fig. 6.14 Single point calibration curve creation screen
6.2.4 Concentration Table

After the ABS values have been entered, a list of concentration and absorbance is completed.
6
6
6
6
6
6
Fig. 6.15 Concentration table screen
CalCurve F1 key;
Displays calibration curve.
Std. Chg F2 key;
Changes, deletes, and adds the standard sample data.
StdPrint
F4 key;
Outputs the concentration table data to a printer. On this occasion, the same data is output to the RS232C port.
UVmini-1240
6-11
6.2.5 Calibration Curve Display
A single point or multi-point calibration curve which has been created can be displayed on the screen
by selecting [CalCurve] in the concentration table screen.
Fig. 6.16 Single point calibration curve display
6
6
6
6
6
6
Fig. 6.17 Multi-point calibration curve display
6
6
6-12
UVmini-1240
The coordinate positions of each standard sample are shown by the x marks.
You can change the values for the concentration and ABS for all of the standard samples and create a
new calibration curve using NewCalib . All standard sample data up to that point will be deleted when
NewCalib is executed.
You can change the order of the calibration curve with Chg.Ord . When data is available at only one
point, this is not displayed.
The equation for the calibration curve will be displayed by Equation .
6.2.6 Changing the Concentration Table

Pressing Std. Chg on the concentration table displays the screen for changing standard sample
concentration and ABS.
6
6
6
Change the data for the

specified No.
Delete the data for the

specified No.
Add data one at a time.
Fig. 6.18 Concentration table change screen

You can Change , Delete or Add standard sample data in the concentration table change screen.
Change F1 key;
Allows you to change the concentration or ABS for the standard sample at the specified No.
Delete F2 key;
Deletes the data at the specified No.
Add F3 F3 key;
Allows you to add concentration and ABS standard samples, one at a time.
6
6
In all of these cases, the calibration curve will automatically be recalculated.
UVmini-1240
6-13
6.3
6.3
Unknown Sample Measurement (Quantitation)
6.3.1 Measurement Screen
When creation of the calibration curve has been completed, you can switch to the measurement
screen using the SmplMeas or the START/STOP key and perform measurements.
(The first measurement will be performed when the START/STOP key is pressed.)
The display format in the measurement screen will differ somewhat depending on the settings for the
No. of measurements and sample control.
Unit
6
Enables redisplay,
printing or deletion, etc.
of measurement data.
Reassignes the next
sample No.
Displays the
calibration curve
equation.
Saves the measurement data to memory.
6
6
Sample printout
6
6
6
(A) Measurement screen (1 cell, 1 measurement)
6-14
UVmini-1240
6.3 Unknown Sample Measurement (Quantitation)
Measure Sample No. 1

five times.
Average value of
measurement Nos. 1
through 5 of Sample
No.1.
Sample printout
6
6
(B) Measurement screen (1 cell, 5 measurements)
6
6
6
6
6
UVmini-1240
6-15
6
No.of measurement = 1
No. of cells = 5
Measurement data
for cells 1 through 5
6
6
6
6
(C) Measurement screen (5 cells, 1 measurement)
Fig. 6.19 Measurement screens and data printout examples
6.3.2 Changing the Sample Number
Pressing the Smpl No. (F1) on the measurement screen changes the No. of the sample to be
measured for the next time.
This function saves measurement data or recalls saved data to the screen.
(See 3.3.3 "Data Management [DataFile]")
6.3.4 Data Display [DataDisp]

On the measurement screen, the data is scrolled up one after another when measurement is
repeated, leaving only eight items of data of the latest measurement displayed.
A list of the measurement data including the ones that have disappeared from the screen is displayed
by this function. Using this function, it is also possible to print the data list or to delete data.
6-16
UVmini-1240
6
Data are displayed
from No. 1.
6
Use this to delete data.
Use this to print data as numeric data.
Fig. 6.20 Data Display screen

Using the
or
key, nine items of data can be scrolled at one time.
Delete F1 key;
This enables you to delete the entire numeric data table at once.
PrintOut F2 F2 key;
This enables you to print all of the data as a numeric data table on the printer (optional). (The PRINT
key on the keyboard will give a hard copy of the screen currently being displayed.)
6
6
6
6
6
6
Fig. 6.21 Printer output
UVmini-1240
6-17
On this occasion, the same data is output to the RS-232C port. The output format of the data is the
same as the print output example shown in Fig. 6.21. At the end of each line, [CR](0Dh) and [LF]
(0Ah) codes are output. At the end of each data, [SB](1Ah) code is displayed.

Baud rate:
Data bit length:
Stop bit:
Parity:
X parameter:
9600 bps
7
1
Odd number (odd)
None
For details of the connection cable, see Chapter 9, 9.1 "Connecting to a PC".
NOTE
The external transmission function comprises simplex transmission of data from the UVmini-1240.
6
6
6
6
6
6
6
6
6-18
UVmini-1240
6.3.5 Display Equation [Equation]
This displays the equation used to compute a calibration curve created as a single point or multi-point
calibration curve. The factor values in the K-factor method are displayed in the parameter
configuration screen.
When using the multi-point calibration curve, relative coefficient r 2 is displayed only at the time of "0
intercept: NO" with a linear equation.
6
Fig. 6.22 Sample calibration curve equation display
Regarding relative coefficient r 2

Relative coefficient r 2 is obtained by the following formula.
With linear equation:
r2=
{ (Ai A) (Ci C) } 2
{ (Ai A)2 } { (Ci C)2 }
6
Ci is concentration of a standard sample
Ai is absorbance of a standard sample
C is mean value of Ci
A is mean value of Ai
6
6
With quadratic or cubic equation (passing the origin = none), R2 obtained by the following formula is
displayed.
R =
{ (Ai A) (Ei E) } 2
Ei = K3Ci3 + K2Ci2 + K1Ci + K0

E = Mean value of Ei
{ (Ai A)2 } { (Ei E)2 }
UVmini-1240
6-19
6.4
6.4
Two/Three-wavelength Calibration method
This is an accurate Calibration method which can be used to eliminate the effects of dispersion due to
interfering components and contaminants and in correcting "floating" of the baseline due to bubbles
when such conditions exist.
6.4.1 Two-wavelength Quantitation

This method quantitates based on the difference between the photometric values at two wavelengths.
This allows for the elimination of the effects of interfering components.
Where B1 and B2 are the absorbances for the target component B at wavelengths 1 and 2, and C1
and C2 are the absorbances for the interfering component C (A1 = B1 + C1, A2 = B2 + C2), when
wavelengths 1 and 2 are selected so that C1 = C2, then A1 A2 = B1 B2 so that only the
information for the target compound is left. Normally, the absorbance wavelength for the target
component is set for 1.
An example of two-wavelength measurement is shown in Fig. 6.23.
Measurement curve A
6
Interfering component C
Target component B
6
6
Fig. 6.23 Schematic diagram of two-wavelength quantitation

Quantitation measurement is then performed according to these parameters.
6.4.2 Three-wavelength Quantitation

6
The following calculation is performed based on the photometric values at three wavelengths.
6
A2 A4
Where, A4 =
( 1 2) A3 + ( 2 3) A1 )
1 3
(See Fig. 6.24) Three-wavelength computation also eliminates the effects of interfering components,
while also being useful in eliminating "floating" of sloped baselines due to dust, etc.
The elimination of the effects of an interfering component will be explained below using Fig. 6.24.
If 1, 2 and 3 are taken so that the points S, T and U of the interfering component are connected by
a single straight line, then A2 A4 = B2 B4 leaving only the information for the target component.
6-20
UVmini-1240
6.4 Two/Three-wavelength Calibration method
Normally, the absorbance wavelength for the target component is set for 2. Quantitation
measurement is then performed according to these parameters.
Measurement curve A
Interfering component C
Target component B
Fig. 6.24 Schematic diagram of three-wavelength quantitation
6
6
6
6
6
6
6
6
UVmini-1240
6-21
6.4 Two/Three-wavelength Calibration method
6
6
6
6
6
6
6
6
6-22
UVmini-1240
7
7 Optional Program Pack
Chapter 7 Optional
Program Pack
7
CONTENTS
7.1
7.2
Starting the Optional Program Pack .................................................................................................. 7-2

Optional Program Pack Auto-start ..................................................................................................... 7-3
7
7
7
7
7
7
UVmini-1240
7-1
7.1
7.1
Starting the Optional Program Pack
Insert the optional program pack into the IC card slot on the front panel of the spectrophotometer main unit.
The optional program starts operating when <4. Optional Program Pack> is selected in the Mode Selection
screen.
The optional program, when used once, is transferred to the internal memory and retained in the
spectrophotometer main unit until another optional program is inserted and executed. Accordingly, if any
program already exists in the main unit, its name is displayed at the line below "Optional Program Pack." In
such an occasion, it is possible to execute the program without inserting the card.
An optional program
pack is selected.
No
Program already exists.
Yes
7
Program is transferred
from the card to the
internal memory.
The same program?
No
Yes
7
7
Update the program?

(dialogue)
No
Yes
Return to the Mode

Selection screen
Program starts.
Fig. 7.1 Flow chart of starting an optional program
7
7
7-2
UVmini-1240
7.2
7.2
Optional Program Pack Auto-start
If <1. Start program> in the <5. Utilities> mode of the mode selection screen has been set to "Optional
Program Pack", a program pack which is inserted into the IC card slot while the power is ON will
automatically be started.
Starting operation is the same as in the previous section.
7
7
7
7
7
7
7
UVmini-1240
7-3
7.2 Optional Program Pack Auto-start
7
7
7
7
7
7
7
7-4
UVmini-1240
8
8 Utilities Mode
Chapter 8 Utilities Mode
8
This is the mode for setting the instrument's operating parameters, such as the light source switching, printer
selection, or the number of data columns displayed.
The parameters which can be set in this mode are parameters which are shared with other modes.
These will also be stored internally, even if the power is turned OFF.
CONTENTS
8.1
8.2
Utilities Screen................................................................................................................................... 8-2

Setting Instrument Parameters .......................................................................................................... 8-3
8
8
8
8
8
UVmini-1240
8-1
8.1
8.1
Utilities Screen
When you select <5. Utilities> in the mode selection screen, the Utilities menu shown in Fig. 8.1 will be
displayed.
Set the number of digit of

data to be displayed.
Set the mode in which the instrument will enter

standby when the power is turned ON.
Set the light source

switching wavelength.
Set the accumulation time
required for reading the
data.
Set the current time.
Set the buzzer sound to
ON/OFF.
Set the printer to be used.
Set the timer to turn the

LCD OFF.
Fig. 8.1 Utilities menu screen
8
8
8
8
8
8
8-2
UVmini-1240
8.2
8.2
Setting Instrument Parameters
<1. Start program>

This function determines the default mode the instrument will use after the power is turned ON
and initialization is completed. Select the item with the cursor key
,
.
Fig. 8.2 Start program selection screen
(1) Standard menu

The instrument is set to this mode before shipment. The mode selection screen will be displayed
when the power is turned ON.
(2) Photometric, Spectrum and Quantitation.
The instrument will standby at the parameter configuration screen of the selected mode.
(3) Optional program pack

The program pack inserted into the slot or the program retained in the internal memory will
automatically start. If no program pack is available, the message saying "Set a card then press
ENTER key." will be displayed.
8
8
(4) Stored param. file

The measurement parameter file stored in instrument memory and selected here will be called up
and the instrument will go to standby mode.
Saving Measurement Parameters...

Save measurement parameters to a file by pressing the [SavParam] function key in the
measurement parameter configuration screen for the respective mode. See 3.3.1 "Save
Parameters [SavParam]" for more details.
8
8
UVmini-1240
8-3
8.2 Setting Instrument Parameters
(5) PC control
The instrument automatically goes to the PC control mode. (See Chapter 9 "PC Control")
<2. Decimal display>

Abs three digits, T% one digit/Abs four digits, T% two digits can be switched for the number of
decimal places to be displayed.
<3. Light source>
Automatic switching:
A deuterium lamp (D2 lamp) is used in the ultra-violet region and a tungsten iodine lamp (WI
lamp) is used in the visible/near-infrared region. The switching wavelength for these two lamps
are set to 340.0 nm at the time of shipment, but it can be set freely between 295.0 nm to
364.0 nm.
WI lamp only:
Regardless of the setting of the measurement wavelength, the WI lamp is always the light
source.
D2 lamp only:
Regardless of the setting of the measurement wavelength, the D2 lamp is always the light
source.
Visible model (UVmini-1240V) cannot select the lamp other than WI.
<4. Data accumulation time>

Designates the accumulation time to read data.
Three options are available: 0.05, 0.1, and 0.2 sec.
The longer the time, the less the dispersion of the obtained data becomes, but the number of data
output per unit time becomes less.
The number of output per second is almost the reciprocal of the accumulation time. Usually, it is
set to 0.1 sec. Changing this setting changes the value of Abs = 0 (100%) as well. Press the
AUTO ZERO key again to obtain Abs = 0.
8
8
<5. Clock set>

Use this to set the time of the clock. The clock uses the 24-hour scheme and is set in the order
(Year/Month/Date Hour: Minute: Second). Since it has a battery back-up, it is not necessary to set
the clock every time you turn ON the power.
Ex.) 10/Dec/94 12:34:56
8
8
<6. Buzzer sound>

Set this to turn ON/OFF of the buzzer.
8
8
8-4
UVmini-1240
<7. Printer setting>

The printer to be connected is selected and the format of a hard copy is set.
(1) Five types of printers can be connected.
Fig. 8.3 Printer selection screen
HCP
Set this when the screen copy printer HCP-1C (optional) is being used.
ESC/P9
Set this when using a printer that supports the ESC/P control code for EPSON 9-pin printers.
ESC/P24
Set this when using a printer that supports the ESC/P control code for EPSON 24-pin printers.
This is also applied for laser printers.
When selecting ESC/P9 and ESC/P24, the left margin can also be set. The setting range is from
0 to 9.
DPU
Set this when the screen copy printer DPU-414(optional) is used.
PCL
Set this when the printer that supports PCL3 of the Hewlett-Packard is used.
8
8
8
8
NOTE
If you do not select the proper printer type, the printer will not operate as expected.
8
(2) Date print
Set this whether the date should be printed in the hard copy output.
(3) Function key print

Set this whether the region to display function keys should be output in a hard copy.
(4) Automatic print (Auto print)
"Auto print" is turned on when selecting "Yes". And when measuring in the photometric,
measurement results are printed on the printer for each measurement. After scanning in the
spectrum, the screen copy is automatically printed.
"Auto print" is turned off when selecting "No", and the above-mentioned operation is not
done. It doesn't function in the quantitation mode. Set the "data print" parameter of the measurement parameter configuration screen in the quantitation mode.
UVmini-1240
8-5
<8. Turning OFF the LCD automatically>

THE LCD is automatically turned OFF when measurement is not performed. The following four
lengths of time are available for the OFF timer setting. When nothing is executed during the set
time, the LCD is turned OFF.
10 min, 30 min, 60 min,
8
8
8
8
8
8
8-6
UVmini-1240
9
9 PC Control
Chapter 9 PC Control
This is the mode in which the UVmini-1240 is controlled by an external computer (PC). An RS-232C interface is
used.
CONTENTS
9.1
9.2
9.3
9.4
9.5
Connecting to a PC ........................................................................................................................... 9-2

Receiving Commands and Protocol .................................................................................................. 9-3
Programming Example ...................................................................................................................... 9-5
Explanation of Commands and Data ................................................................................................. 9-9
Command List ................................................................................................................................. 9-10
9
9
9
9
9
UVmini-1240
9-1
9.1
9.1
Connecting to a PC
Use the cable specified in the wiring specifications below as the RS-232C cable that connects the
spectrophotometer to a PC. Connect the RS-232C port on the right side of the spectrophotometer to
the RS-232C port on the PC.
D-sub 9-pin male

connector on PC
RXD
TXD
DTR
GND
DSR
DTS
CTS
D-sub 9-pin male

connector on Spectrophotometer
1
2
3
4
5
6
7
8
9
2
3
7
TXD
RXD
Signal GND
Fig. 9.1 RS-232C cable for IBM

The part number of this RS-232C cable is 200-86408.
A total of 3 wires are actually used: 2 for input/output and 1 for ground.
The connector for connecting to the PC, when seen from the PC side as above, has the control wire
pins connected to each other so that input and output are always enabled.
9
9
9
9
9
9-2
UVmini-1240
9.2
9.2
Receiving Commands and Protocol
When you select PC Ctrl

F4 in the mode selection screen, the following screen is displayed. It
becomes possible to communicate with PC via the RS-232C port.
Fig. 9.2 PC control mode screen

The exchange of signals (communication) with the PC must be performed with one being the
"speaker" and the other the "listener". In this case, the speaker will be referred to as the master and
the listener as the slave.
The exchange of signals is performed under a set procedure (protocol). These signals comprise not
only commands and data, but also codes for the control of the procedure (control codes). The control
codes shown in the table below are used in the exchange of signals between the UV and a PC.
Table 9.1 Control Codes
Control Code
(Hexadecimal)
ENQ ($05)
(Enquiry)
Direction
Master to Slave
Function
Enquiry code sent when you wish to send
commands or data. In particular, the first ENQ of
a series of transactions also indicates the start of
communication.
EOT ($04)
(End of Transmission)
Master to Slave
Code for announcing the end of communication.

Use this when there are no more data to be sent.
ESC ($1B)
(Escape)
Bi-directional
Code sent when you wish to interrupt

communication.
ACK ($06)
(Acknowledge)
Slave to Master
Code returned from receiving side in affirmative

response to a command, data or code which has
been sent.
NAK ($15)
(Negative Acknowledge)
Slave to Master
Code returned from receiving side in negative

response to a command, data or code which has
been sent.
NUL ($00)
(Null)
Master to Slave
9
9
9
9
9
Code for recognizing the end of a variable-length

signal, such as a command or data, etc. This is
also called the terminator.
UVmini-1240
9-3
9.2 Receiving Commands and Protocol
The transmission parameters are as follows.

Transmission rate: 9600 bps
Data bits:
7 bits
Stop bit:
1 bit
Parity bit:
Odd
The types of commands sent from the PC to the UV can be generally classified as follows according to
the direction of the data flow.
a)Write command... Allows the status of the UV to be set.
b)Read command... Allows the status of the UV to be recognized.
The procedures for these commands have several types. A time chart is shown below. In the figure,
the "----" mark indicates the master. Please note that the master and slave roles alternate in the
communication process.
a. Write command... Protocol A
E
N
Q
PC
COMMANDS
N
U
L
A
C
K
A
C
K
UV
A
C
K
Write
Status
E
O
T
Time to send
one code
b. Read command... Protocol B, Protocol B'
9
PC
9
UV
E
N
Q
COMMANDS
A
C
K
A
C
K
Read
Status
E
N
Q
A
C
K
A
C
K
A
C
K
N
U
L
N
DATA U
L
E
O
T
There are also repeating commands in this portion

This is Protocol B'.
The write command in a) is protocol A, the type in which data are received only once from the
external computer at the read command in b) is protocol B, and when there are multiple data
received it is called protocol B'.
9-4
UVmini-1240
9.3
9.3
Programming Example
This section describes in detail the procedures of three transmission types: protocol A, protocol B, and
protocol B'.
N in the description stands for the number of repetitions. It is 5 in this example.
Checking for the number of repetitions and time-out setting are omitted in the flow chart.
Protocol A
(1) Establishing communication link
1 in Flow Chart 1
Prior to sending command from the master station (PC in this case), the ENQ code is sent to
make the slave station (spectrophotometer in this case) prepare for reception of command codes.
The slave station returns ACK to confirm that reception of the command data is ready.
<When abnormality occurs:>
When NAK is returned for ENQ, ENQ is sent again. If NAK is returned for Nth time re-sending
(5 times in this case) of ENQ, it is judged that abnormality has occurred at the slave station
and communication ends.
If any other code than ACK and NAK is returned, it is ignored and the master waits for the next
response.
If no response is made after a certain period of time, ENQ is sent again. If response is not
made for the Nth time re-sending, it is judged that abnormality has occurred at the slave
station and communication ends.
(2) Sending command data
2 in Flow Chart 1
When establishment of the communication link is correctly completed, the master station (PC)
sends command data. When the slave station (spectrophotometer) receives the data correctly,
ACK is returned.
The master station and the slave station are reversed on this occasion.
If NAK is returned for the command data, the previously sent data is sent again. If NAK is
returned for Nth time re-sending, it is judged that abnormality has occurred at the slave station
and communication ends.
When any code other than ACK and NAK is returned, it is ignored and the master waits for the
next ENQ.
If no response is made for the Nth time sending, it is judged that abnormality has occurred at
the slave station and communication ends.
9
9
(3) Ending
3 in Flow Chart 1
The master station (spectrophotometer in this case) processes the command data and sends
EOT to the slave station (PC in this case) when processing ends. The slave station waits until
EOT is sent and returns ACK when EOT is sent. This completes communication.
9
9
9
UVmini-1240
9-5
9.3 Programming Example
Flow Chart 1
Start
Sending ENQ
Receiving character by character
No
ACK received ?
No
NAK received ?
Yes
Yes
Sending command character string
ACK received ?
No
No
Yes
9
EOT received ?
Yes
Sending ACK
End
9
9
9
9
9-6
UVmini-1240
No
NAK received ?
Yes
Protocol B and protocol B'
(1) Establishing communication link

Same as that of protocol A.
(2) Sending command data
Same as that of protocol A.
(3) Receiving answer-back data
1 in Flow Chart 2
The master station (spectrophotometer in this case) processes the command data and when
processing ends, it sends ENQ code to make the slave station (PC) ready for data reception. The
slave station waits until it receives ENQ and returns ACK to inform the master station
(spectrophotometer) that the slave is ready for data reception. The master station
(spectrophotometer) receives this ACK and starts data transmission.
With protocol B', there is more than one data transmission. The slave station (PC) sends ACK for
each data transmission to inform the master station of data reception.
If no answer-back data is sent after a certain period of time, ENQ is sent again. If no data is
returned for Nth time re-sending, it is judged that abnormality has occurred at the master
station spectrophotometer) and ends communication.
While the character string is being received character by character, if the next character is not
received after a certain period of time, NAK is returned immediately.
(4) Interrupting data reception
2 in Flow Chart 2
With protocol B', in order to interrupt data reception from the slave station (PC in this case) while
it is receiving more than one data transmission, ESC is sent instead of ACK. The master station
(spectrophotometer) cancels data transmission and communication link ends. Also, if data cannot
be sent due to occurrence of abnormality while the master station (spectrophotometer) is sending
data, ESC is sent instead of the data and communication link ends.
(5) Ending
3 in Flow Chart 2
The master station (spectrophotometer) sends EOT after sending all the data. The slave station
(PC) judges the end of data reception by receiving EOT and send ACK to end transmission.
9
9
9
9
9
UVmini-1240
9-7
Flow Chart 2
Start
Sending ENQ
ACK received ?
No
No
NAK received ?
Yes
Yes
Sending command character string
No
ACK received ?
No
NAK received ?
Yes
Yes
9
ENQ received ?
No
Yes
Sending ACK
9
Receiving data
No
9
EOT received ?
Yes
Sending ACK
End
9-8
UVmini-1240
No
ESC received ?
Yes
9.4
9.4
Explanation of Commands and Data
The commands which can be sent from an external computer are made up of the following elements.
Command code
Commands
Parameter
Terminator
A command code consists of a single lower-case alphabetic code. The number of a parameter
depends on the command code. Commands can be divided into those with no parameter, those with
only one parameter and those with multiple parameters. When there are multiple parameters, it is
necessary to separate the parameters with a symbol (delimiter). "," (comma) is used as the delimiter.
Since all parameters are sent as ASCII text, if you wish to set the number 15, this would be expressed
in hexadecimal as $31,$35. NUL is used as the terminator.
Data which is sent from the spectrophotometer have the following structure.
Data code
Data
Parameter
Terminator
A data code consists of a single lower-case alphabetic code, the same as a command code. There is
only one parameter, reflected by a text string. If the parameter is 10.36, it would be expressed in
hexadecimal as $31,$30,$2E,$33,$36. NUL is used as the terminator.
9
9
9
9
9
UVmini-1240
9-9
9.5
9.5
Command List
The terminator symbol has been omitted from the command format places in the command list. When
sending to the UVmini-1240, send a terminator code (NUL) right after the content shown in the table
as the actual command. "n", "m" and "t" in the command format indicate parameters. The protocol
types are A, B and B' and correspond with the time chart in 9.2.
Protocol A: Writing command
Protocol B: Reading command
Protocol B': Reading command with more than one data to be obtained
Table 9.2 Command List

Command
format
Protocol
type
Name
Description of processing and precautions
an, m, u
Measurement
Performs wavelength scan. The measured data

is saved in the continuous data storage area
inside the spectrophotometer. To take data in,
use the [f] command (file data transfer) as well.
Parameters n and m correspond to the starting
wavelength and the ending wavelength
respectively.
The parameter is set to the value of wavelength
multiplied ten times. For example, 500.0 nm is
5000 and 765.4 nm is 7654. Set n and m so that
they satisfy the following conditions.
1900 n,m 11000
n-m 100
Parameter u designates the measurement
speed. They correspond respectively as follows.
u = 1: Very fast
u = 2: Fast
u = 3: Medium
u = 4: Slow
u = 5: Very slow
Measurement pitch is determined according to
Table 9.3 Relationship of the measurement
command parameters.
9
9
9
*continued.
Table 9.3 Relationship of the measurement command parameters
Wavelength difference in the measured wavelength (nm)

u
Less than 100

nm
Less than 200

nm
Less than 500

nm
500 nm or more
1: Very fast
0.2 nm
0.2 nm
0.5 nm
1.0 nm
2: Fast
0.1 nm
0.2 nm
0.5 nm
1.0 nm
3: Medium
0.5 nm
0.5 nm
0.5 nm
1.0 nm
4: Slow
0.2 nm
0.2 nm
0.5 nm
1.0 nm
5: Very slow
0.1 nm
0.2 nm
0.5 nm
1.0 nm
9-10
UVmini-1240
9.5 Command List
Table 9.2
Command
format
Protocol
type
Name
bn, m
Time scan
Performs time scan. The measured data will be saved in the

continuous data storage area inside the spectrophotometer.
To take data in, use the [f] command (file data transmission)
as well.
Parameter n designates measurement time. Set it satisfying
the following condition.
1 n 6500
Parameter m designates the unit of measurement time.
Setting conditions are as follows.
m = 0: second
m = 1: minute
cn, m
fn
B'
Baseline correction Performs baseline correction. Parameters n and m

correspond to the starting wavelength and the ending
wavelength respectively.
The parameter is set to the value of wavelength multiplied
ten times. Set n and m so that they satisfy the following
conditions.
1900 n,m 11000
n m 100
Current data
File data transfer
Outputs the current data. When this command is sent, the

spectrophotometer performs one measurement and outputs
the data as follows.
dk
Parameter k is the current data and formatted as follows
according to the measurement mode.
At ABS:
x.xxxy
Besides ABS: xxx,xy
The sign of the parameter code is output when it is negative.
When it is positive, a space is output. Also, y is output only
when the number of data display digits set in the Utilities
mode of the spectrophotometer is Abs(4), T%(2).
9
9
The data saved in the storage area of the spectrophotometer

is taken in by the [a] command (scan measurement) or the
[b] command (time scan measurement). Parameter n is the
number of data items you wish to take in. The data from the
beginning to n can be taken in. If the value larger than the
data items saved in the storage area is set, the processing
will end at the point where you ran out of data. n must satisfy
the following condition.
1 n 2000
The data to this command is output as follows.
zzzz.z x.xxxy
zzzz.z xxx.xy
z indicates wavelength (time), x and y indicate measurement
data, and indicates "space" data. The code for the
measured data is output when it is negative only. When it is
positive, a space is output. Also, y is output only when the
number of data display digits set in the Utilities mode of the
spectrophotometer is Abs(4), T%(2).
9
9
9
*continued.
UVmini-1240
9-11
9.5 Command List
Table 9.2
Command
format
Protocol
type
Name
gn
Gain setting
jn
In
mn
on, m, t

Sets the gain when energy is measured.
Parameter n must satisfy the following conditions.
1n6
Data accumulation 1Sets the data accumulation time to obtain one item of data.
time Parameter n corresponds with accumulation times as
follows.
n = 1: 50 msec
n = 2: 100 msec
n = 3: 200 msec
Light source
setting
Sets the light source for energy measurement. Parameter n

corresponds with light sources as follows.
n = 1: WI lamp
n = 2: D2 lamp
n = 3: Optional lamp
Multi-cell selection Changes the accessories of multi-cell and, if required,

initializes it.
Parameter n corresponds with multi-cells as follows.
n = 1: standard cell
n = 2: 6-position cell
n = 10: CPS
9
9
9
9
9
Syringe sipper
Controls the syringe sipper. This command is valid when a

syringe sipper is connected to the sample compartment.
Parameter n designates the operation mode.
n = 1: Initialization
n = 2: Sip (Valve at the sample side.)
n = 3: Purge (Valve at the drain side.)
n = 4: Purge (Valve at the sample side.)
Parameter m designates the sip speed.
It corresponds with speed as follows. The unit is ml/sec.
m = 1: 1.2
m = 2: 0.6
m = 3: 0.3
m = 4: 0.2
m = 5: 0.1
Parameter t designates the capacity.
The following condition must be satisfied. The unit is 0.01 ml.
0 t 1000
Parameter m and parameter t are valid when operation
mode n is 2, 3, or 4.
Although, set a value also in mode 1, Initialization.
Example 1: Initialization
o1, 1, 1
Example 2: Feeding 5ml from the sample side to the syringe
at the speed of 0.6 ml/sec.
o2, 2, 500
*continued.
9-12
UVmini-1240
9.5 Command List
Table 9.2
Command
format
Protocol
type
Name
pn, m, t
Sipper 160
Controls the Sipper 160. This command is valid when the

Sipper 160 is connected to the sample compartment of the
spectrophotometer.
Parameter n designates operation mode.
n = 1: Initialization
n = 2: Sip
n = 3: Purge
Parameter m designates sip speed.
It corresponds with speed as follows.
m = 1: Fast
m = 2: Medium
m = 3: Slow
Parameter t designates rotation time.
Satisfy the following condition. The unit is second.
64 t 64
Parameter m and parameter t are valid when operation
mode n is 2 or 3.
Although, set a value also in mode 1, Initialization.
Example 1: Initialization
p1, 1, 1
Example 2: Feeding from the sample side to the sipper for
ten seconds at high speed. p2,1,10
qn
Move cell position
Moves the cell position of the multi-cell or the CPS-240.

Parameter corresponds to the direction of cell's movement
as follows.
n = 1: Move one cell forward.
n = 2: Move one cell backward. (CPS-240)
Moves to Cell 1. (Multi-cell)
This command is valid when the multi-cell or the CPS-240 is
connected to the sample compartment of the
spectrophotometer.
9
9
Check cell position Checks the present cell position of the multi-cell or the CPS240. When the command is executed, the data is returned
from the spectrophotometer as follows.
qk
Parameter k corresponds to the cell position No. as follows.
With the 6-position cell or with the CPS-240: 1 n 6 This
command is valid when the multi-cell or the CPS-240 is
connected to the sample compartment of the
spectrophotometer.
9
9
9
*continued.
UVmini-1240
9-13
9.5 Command List
Table 9.2
Command
format
Protocol
type
Name
ASC nozzle
Checks the nozzle condition of the auto-sample changer,

ASC-5. If the nozzle is lowered according to this, sample
absorption can start. When the command is executed, the
data is returned from the spectrophotometer as follows.
rk
Parameter k is a nozzle condition number and it corresponds
with conditions as follows.
k = 1: Nozzle is lowered.
k = 2: Nozzle is raised.
This command is valid when the ASC-5 (optional) is
connected.
sn
Syringe sipper
lamp
Turns ON/OFF the syringe sipper lamp.

This command is valid when the syringe sipper is connected
to the sample compartment of the spectrophotometer.
Parameter n corresponds with the lamp condition as follows.
n = 0: lamp OFF
n = 1: lamp ON
vn
Measurement
mode
Sets the measurement mode. Parameter n corresponds with

the mode number as follows.
n = 1: Absorbance (Abs)
n = 2: Transmittance (T%)
n = 3: Energy
wn
Wavelength setting Sets wavelength. The value of the set wavelength multiplied
10 times is used for parameter n. For example, to set to
500.0 nm, use 5000. n must satisfy the following condition.
1900 n 11000
9
9
9
9
9-14
UVmini-1240
Auto zero
Performs auto-zeroing (to make the absorbance zero or to

make the transmittance 100% under the current conditions).
10
10 File Transfer
Chapter 10 File Transfer
This is the mode used to transfer the data files and condition files inside the memory or in the data pack of the
spectrophotometer to an external computer (PC). Use the PC software, "UV Data Manager" (optional) that
controls UV data.
CONTENTS
10
10.1 Connecting to a PC ......................................................................................................................... 10-2

10.2 Operation Method ............................................................................................................................ 10-3
10
10
10
10
UVmini-1240
10-1
10.1
10.1
Connecting to a PC
For information on how to connect the spectrophotometer to an external computer and the RS-232C interface
cable, see Chapter 9 "PC Control".
10
10
10
10
10
10-2
UVmini-1240
10.2
10.2
Operation Method
When FileTrns F3 key is selected in the Mode Selection screen, the following screen is displayed.
From this state, communication is possible via the RS-232C port, and the UV Data Manager becomes
available.
10
Fig. 10.1 File transfer screen

To return from File Transfer mode to the Mode Selection screen, press the RETURN key. Or how to use the
software on the PC, the UV Data Manager, see the instruction manual of the UV Data Manager.
10
10
10
10
10
10
UVmini-1240
10-3
10.2 Operation Method
10
10
10
10
10
10
10-4
UVmini-1240
11
11 Maintenance & Checking
Chapter 11 Maintenance &

Checking
CONTENTS
11.1
11.2
11.3
11.4
Daily Maintenance & Periodic Maintenance .................................................................................... 11-2

Initialization & Error Display............................................................................................................. 11-3
Troubleshooting ............................................................................................................................... 11-5
Replacing Light Source ................................................................................................................... 11-6
11.4.1 Light Source Specifications ................................................................................................ 11-6
11.4.2 Light Source Replacement Procedure................................................................................ 11-7
11.5 Replacing Fuses ............................................................................................................................ 11-11
11.6 List of Consumable Parts, Spare Parts ......................................................................................... 11-13
11
11
11
11
11
UVmini-1240
11-1
11.1
Daily Maintenance & Periodic Maintenance
11.1
(1) Cleaning Sample Compartment (Daily)

When handling large numbers of liquid samples, check the floor of the sample compartment for
spilled solution samples. Wipe up spilled samples immediately as described in Section 1.2.2
"Cleaning the Unit".
11
NOTE
Please be aware that if spilled samples are left, they may evaporate and the vaporized gas will fill
the light path in the sample compartment, corroding the interior and causing inaccurate
measurement results.
(2) Checking Baseline Flatness (Monthly)
Inspect the flatness of the baseline according to the procedure in Chapter 1 "1.5.1 Baseline
Flatness". If there is an abnormality in the baseline (the curve is greater than 0.010Abs), correct
the instrument baseline.
(3) Checking Wavelength Accuracy (Monthly) *
Inspect the wavelength accuracy according to the procedure in Chapter 1 "1.5.2 Wavelength
Accuracy". If there is an abnormality in the wavelength accuracy (the peak wavelength shift is
greater than 1.0 nm), contact your Service Representative.
11
11
11
11
11
11-2
UVmini-1240
11.2
11.2
Initialization & Error Display
When the power switch is turned ON, the spectrophotometer performs various checks and initial
settings in the order shown in the table on Fig. 11.1, and if everything is normal, initialization is
completed after about 4.5 minutes. The result of each step will be displayed as it is completed. If a
step is properly completed, "OK" will be displayed. However, if any kind of abnormality is detected,
"NG" will be displayed. If a "NG" message is displayed, inspect the instrument according to the check
point items in the table. If the problem is still unclear, contact your Service Representative and
describe the displayed error message.
11
UVmini-1240V
UVmini-1240
Fig. 11.1 Initialization screen
11
11
11
11
11
UVmini-1240
11-3
11.2 Initialization & Error Display
Table 11.1 Initialization and Errors
11
Display
Description
LSI Initialize
Initialize each I/O
ROM Check
Check program ROM
RAM Check
Check memory elements (RAM)
Filter Origin
Detect reference position of filter.
WL Motor Org.
Detects mechanical wavelength

origin position.
Lamp Motor Org.
Detect reference position of motor

that drives light source switching
mirror.
WI Lamp Energy
Checks whether or not the WI lamp

(tungsten iodine lamp) light energy
is at full level.
0 Order Light
Checks 0-order light which is the

optical origin
D2 Lamp Energy
(UV-Visible Model)
Checks whether or not the D2 lamp

(deuterium lamp) light energy is at
sufficient level.
10
Check
(UV-Visible Model)
Checks wavelength by detecting

the intensity line at 656.1 nm using
the D2 lamp.
11
Remedial Action
Try turning the power OFF and then
ON again. If the same error occurs
again, contact your Service
Representative.
Remove the light source

compartment cover and check if
the WI lamp is lit. If it is not lit, try
turning the power ON again.
If it still does not light, the lamp
must be replaced.*1
Check to see if there is something
in the sample compartment that is
obscuring the light.
Remove the light source
compartment cover and check if
the D2 lamp is lit. If it is not lit, try
turning the power ON again. If it still
does not light, the lamp must be
replaced.*1
Check to see if there is something
in the sample compartment that is
obscuring the light.
*1 Refer to Fig. 11.4 and Fig. 11.5 in section 11.4.2 to remove the light source compartment cover.
11
11
11
11-4
UVmini-1240
11.3
11.3
Troubleshooting
There are many instances in which the spectrophotometer will not operate properly as the result of
consumable part deterioration, an operational error or neglect of maintenance and inspection. Please
check the following items. If the cause of operation failure is still unknown after this inspection, contact
your Service Representative.
11
(1) Does not operate at all. Nothing appears on the LCD unit screen.
Checking Point
1. Is the power cord securely connected?
2. Is the fuse blown?
Remedial Action
Securely connect the cord.
Replace the fuse.
100 V to 120 V area: 4.0 A
220 V to 240 V area: 2.0 A
(2) After power is ON, error message is displayed during initialization.

Inspect the check points listed in "Table 11.1 Initialization and Errors".
(3) Numbers cannot be entered from the keyboard.
Checking Point
1. Is an incorrect value being entered?
Example GOTO WL=1150 nm
2. Is the computer IC or the keyboard
defective?
Remedial Action
Enter the correct value.
Contact our Service Representative.
(4) Photometry values are odd.

Checking Point
1. Did you press mistakenly the
AUTO ZERO key during measurement?
2. Sample processing error
3. Is the cell being used appropriate?
Remedial Action
Return to the blank condition (reference
condition) and press the AUTO ZERO key
again. Perform proper processing.
Do not use a glass cell in the ultraviolet range.
11
(5) Baseline curve does not meet normal specifications.

Checking Point
1. Is the beam restricted?
2. Are you using optional accessory?
Remedial Action
Some of the unit specifications may not be met
when the beam is restricted, or certain optional
accessories are installed.
11
11
11
11
UVmini-1240
11-5
11.4
Replacing Light Source
11.4
11.4.1 Light Source Specifications
11
Table 11.2 Light Source Specifications
Part No.
Type
Average life
Characteristics
Shape
WI Lamp
D2 Lamp *
062-65005
062-65055-05
55134
L-6380
2000 hours
500 hours
12V 20W
Discharge voltage:
75~95V
Discharge current:
300mA
Discharge start voltage: 230~350V
Fig. 11.2 (a)
Fig. 11.2 (b)

Protrusion
11
Beam port
(a) WI Lamp
Beam port
(b) D2 Lamp
Fig. 11.2 Light source appearances
11
11
CAUTION
A deuterium lamp (D2) lamp radiates ultraviolet rays harmful to eyes and skin.
Do not stare at the lamp directly while it is lit.
11
11
11-6
UVmini-1240
11.4 Replacing Light Source
11.4.2 Light Source Replacement Procedure
11
WARNING
HOT SURFACE
The light source and light source compartment both get very hot. To change a light source, turn off
the power and then change the light source only after checking to see that it has cooled sufficiently.
CAUTION
1. Please wear gloves when handling the light source so as not to leave fingerprints on the beam
port of the new light source. A fingerprint will burn onto the bulb, and when the light source gets
hot, light transmission will deteriorate.
CAUTION
2. Be especially careful when removing and mounting the light source compartment cover so that
the back of the cover does not strike the protrusion on the top of the D2 lamp (deuterium lamp).
Such a hit could cause a leak in the tube's vacuum.
11
11
11
11
11
1WI lamp
2WI lamp socket
3WI lamp retainer spring
4D2 lamp
5Lamp change mechanism
Fig. 11.3 Interior of light source compartment
UVmini-1240
11-7
Replacing the D2 lamp
11
(1) Using a Philip's screwdriver, remove the screws at the rear of the main unit shown in Fig. 11.4.
Philip's screwdriver
Screw
Fig. 11.4 Light source cover

(2) Next, pull the light source cover in the direction of the arrow in Fig. 11.5 to remove the light source
cover.
11
11
11
11
Fig. 11.5 Removing the light source cover
11-8
UVmini-1240
(3) Slowly extract the D2 lamp upward and remove it from its socket.
11
(4) Insert the new D2 lamp in the socket. Check at that time to see that it is well seated.
(5) Turn ON the power.
(6) Press the GOTO WL key and set the wavelength to 250 nm.
(7) Check to see that the beam is shining into the monochromator entrance slit properly. The
spectrophotometer will automatically make horizontal adjustments. (The center of the beam does
not necessarily have to match the center of the slit.) If the lamp has not been replaced properly,
the beam may not match with the entrance slit in the vertical direction.
(8) Close the cover of the light source compartment and be sure to tighten the screws. Do not use
the unit with the cover of the light source compartment removed.
Replacing the WI lamp
(1) Press on the parts indicated by the arrows in Fig. 11.4 on the sides of the light source cover, lift
the cover up and release the "catch".
(2) Next, pull the light source cover in the direction of the arrow in Fig. 11.5 to remove the light source
cover.
(3) Remove the WI lamp retainer spring from the end of the top of the WI lamp. In case of the UVVis
Model since there is fear of touching the D2 lamp with your hand at this time, either cover the D2
lamp with a clean piece of paper or cloth or remove the D2 lamp before performing this operation.
(Refer to "Replacing D2 Lamp" for the procedure of removing the D2 lamp.)
11
Fig. 11.6 Removing the WI lamp
11
(4) Extract the WI lamp from its socket.

(5) Please wear gloves so as not to contaminate the beam port of the WI lamp. Then, holding the
new WI lamp so as to grasp it from the top and bottom, insert it into its socket.
11
(6) Insert the protrusion at the top of the WI lamp into the hole in the retainer spring so that the WI
lamp does not fall out.
11
(7) Reset the previously removed D2 lamp. Refer to "Replacing D2 Lamp" for the method of resetting
the D2 lamp.
11
UVmini-1240
11-9
(8) Turn ON the power.
11
(9) Check to see that the beam is shining into the monochromator entrance slit properly. The
spectrophotometer will automatically make horizontal adjustments. (The center of the beam does
not necessarily have to match the center of the slit.) If the lamp has not been replaced properly,
the beam may not match with the entrance slit in the vertical direction.
(10) Close the light source compartment cover and be sure to tighten the screws. Do not use the unit
with the light source compartment cover removed.
11
11
11
11
11-10
UVmini-1240
11.5
11.5
Replacing Fuses
11
WARNING
ELECTRICAL SHOCK
There is possibility of electric shock. Before replacing the fuse, turn OFF the power and unplug the
power cord.
When the fuse burns out, replace it by the following procedure. Use only the specified fuse.
(1) Turn OFF the power.
(2) Remove the power cord from the power connector.
(3) Use a standard screwdriver to open the cover on the fuse holder, as shown in Fig. 11.7.
Cover of fuse holder
Standard screwdriver
Fig. 11.7 Opening the fuse holder cover
11
(4) Pull out the fuse holder, as shown in Fig. 11.8.
11
11
11
Fuse
11
Fig. 11.8 Interior of fuse holder

(5) Remove the old fuse from the fuse holder and replace it with the new fuse. Of the two fuses, you
need only replace the burned out fuse. (If both fuses are burned out, replace both fuses.)
UVmini-1240
11-11
11.5 Replacing Fuses
(6) Replace the fuse in the fuse holder.
11
(7) Close the fuse holder cover until you hear its snap shut.
NOTE
After replacing the fuse, turn ON the power switch and check to see that the unit initializes properly.
CAUTION
Pay attention to the rating and type of the fuse.
Be sure to replace with the fuse of the specified rating and type.
Power supply voltage 100V to 120V: Part No. 072-02004-22
250V 4A T (5 20)
Power supply voltage 220V to 240V: Part No. 072-02004-19
250V 2A T (5 20)
11
11
11
11
11-12
UVmini-1240
11.6
11.6
List of Consumable Parts, Spare Parts
Part Name
Model
11
Part No.
Tungsten iodide lamp
55134
062-65005
Deuterium lamp *
L-6380
062-65055-05
Cover, sample compartment
206-89180
Cell holder assembly
206-89184-91
Screw, knurl
204-00570
Fuse (for 100V to 120V)
250V 4A T (5 20)
072-02004-22
Fuse (for 220V to 240V)
250V 2A T (5 20)
072-02004-19
AC power cord (for 100V to 120V)
071-60816-12
AC power cord (for 220V to 240V)
071-60825-51
* for UVmini-1240 (UV-Vis Model)
11
11
11
11
11
UVmini-1240
11-13
11.6 List of Consumable Parts, Spare Parts
11
11
11
11
11
11-14
UVmini-1240
12
12 Specifications
Chapter 12 Specifications
CONTENTS
12.1 Hardware Specifications .................................................................................................................. 12-2
12.2 Software Specifications ................................................................................................................... 12-4
12
12
12
12
UVmini-1240
12-1
12.1
12.1
Hardware Specifications
UVmini-1240 (UV-Vis)
Measurement Wavelength Range
12
12
Spectral Band Width (Resolution)
5 nm
Wavelength Display
0.1 nm units
Wavelength Setting
0.1 nm units (1 nm units in scan)
Wavelength Accuracy
1.0 nm
Wavelength Repeatability
0.3 nm
Wavelength Scanning Speeds
GOTO WL command:approx. 3800 nm/min

Wavelength scan:24 ~ 1400 nm/min
Light Source Switching
Automatic switching with wavelength range. Can be set anywhere in

the range from 295 nm to 364 nm by the unit of 1 nm. (340 nm
is recommended.)
Stray Light
Less than 0.05%
Photometric System
Single beam optics
Photometric Range
Absorbance:0.3 ~ 3.0Abs (when uncorrected baseline curve is

within 0.5Abs.)
Transmittance:0 ~ 200%
Recording Range
Absorbance:3.99 ~ 3.99Abs
Photometric Accuracy
0.005Abs (at 1.0Abs)

Photometric repeatability
Auto Zero Function
[AUTO ZERO] key enables one-touch setting
Baseline Stability
0.001 Abs/h
Baseline Flatness
0.010 Abs
Noise Level
0.002 Abs (0.0005Abs RMS)
Baseline Correction
Automatic correction using computer memory
Light Source
20W halogen lamp (long-life 2000 hour), Deuterium lamp (sockettype), On-board automatic light source positioning mechanism
Monochromator
Uses concave holographic grating
Detector
Silicon photodiode
LCD
Backlit (320 240 dot), Adjustable contrast
Sample Compartment
Interior dimensions 110 230 105 (mm)

(W D H) (Partial depth 155 mm)
Removable type 2-screw attachment
Beam dimensions 2 7 mm (W H)
(at center of accessory cell holder)
Power Supply
100V system (100V to 120V) P/N 206-89175-92

200V system (220V to 240V) P/N 206-89175-38
50/60Hz 160VA
Dimensions
416 379 274 (mm) (W D H)
12
12
12-2
190 ~ 1100 nm
Weight
11 kg
Ambient Temperature
Room temperature 15 ~ 35C
Ambient Humidity
Humidity 45 ~ 80% (less than 70% at temperature higher than

30C)
UVmini-1240
12.1 Hardware Specifications
UVmini-1240 (Visible)
Measurement Wavelength Range
Spectral Band Width (Resolution)
12
325 ~ 1100 nm
5 nm
Wavelength Display
0.1 nm units
Wavelength Setting
0.1 nm units (1 nm units in scan)
Wavelength Accuracy
1.5 nm
Wavelength Repeatability
0.3 nm
Wavelength Scanning Speeds
GOTO WL command:approx. 3800 nm/min

Wavelength scan:24 ~ 1400 nm/min
Light Source Switching
Automatic switching with wavelength range. Can be set anywhere in

the range from 295 nm to 364 nm by the unit of 1 nm. (340 nm
is recommended.)
Stray Light
Less than 0.05%
Photometric System
Single beam optics
Photometric Range
Absorbance:0.3 ~ 3.0Abs (when uncorrected baseline curve is

within 0.5Abs.)
Recording Range
Absorbance:3.99 ~ 3.99Abs
Photometric Accuracy

Photometric repeatability
Auto Zero Function
[AUTO ZERO] key enables one-touch setting
Baseline Stability
0.001 Abs/h
Baseline Flatness
0.010 Abs
Noise Level
0.002 Abs (0.0005Abs RMS)
Baseline Correction
Automatic correction using computer memory
Light Source
20W halogen lamp (long-life 2000 hour)

On-board automatic light source positioning mechanism
Monochromator
Uses concave holographic grating
Detector
Silicon photodiode
LCD
Backlit (320 240 dot), Adjustable contrast
Sample Compartment
Interior dimensions 110 230 105 (mm)

(W D H) (Partial depth 155 mm)
Removable type 2-screw attachment
Beam dimensions 2 7 mm (W H)
(at center of accessory cell holder)
Power Supply
100V system (100V to 120V) P/N 206-54000-92

200V system (220V to 240V) P/N 206-54000-38
50/60Hz 160VA
Dimensions
416 379 274 (mm) (W D H)
Weight
11 kg
Ambient Temperature
Room temperature 15 ~ 35C
Ambient Humidity
Humidity 45 ~ 80% (less than 70% at temperature higher than

30C)
UVmini-1240
12
12
12
12
12-3
12.2
12
12
12-4
Software Specifications
Photometric
(1)
(2)
(3)
(4)
(5)
Spectrum
(1) Spectrum measurement

Measurement mode: ABS; T%, E
Scan speed: Very fast medium, slow, very slow
Repetition times of scan: 1 to 99
Recording method: Overlay/Sequential is selectable.
(2) Spectrum data processing function
Detection of peaks/valleys (up to 20 for both)
Enlarge/Reduce (Reduction is available with the vertical axis only.)
Data loading by cursor keys
Data saving/loading function (main unit: 6, data pack: 21)
(3) Function of transferring spectrum data via RS-232C port to an external PC
(4) When ESC/P printer is used: waveform printing of spectrum (A5 size output)
Quantitation
(1) One-wavelength measurement, two-wavelength/three-wavelength measurement

(2) Function relating to calibration curve
Automatic calculation of concentration by K factor method
Automatic calculation of concentration by single point calibration curve method
Multi-point calibration curve
Number of standard samples (2 to 10)
Calibration curve: 1st- to 3rd-order regression calibration curve
Selection of the original passing
Repetition measurement (1 to 10 times) of standard samples and creation of calibration
curve according to its mean measurement value
Display of calibration curve formula
Display of relative coefficient of calibration curve
(3) Measurement
Quantitation by repetition measurement (1 to 10 times) and its mean measurement value
(4) Saving/loading function of table data
(5) Automatic data printing function/Automatic data output function to RS-232C port
(6) With the CPS-240 or multi-cell (both options) is in use: Sequential measurement up to six
cells is possible.
Shared
functions
(1) Selection function of the setting condition after power is turned ON.
Stand-by mode can be designated to enter any parameter configuration screen among
the Photometric, Spectrum, and Quantitation modes. Also, it can be designated by a
parameter file.
(2) Backup of the previous measurement conditions
(3) Selection function of the number of decimal places
The number of decimal places for absorbance can be switched to three digits or four
digits.
(4) Buzzer ON/OFF function
(5) Automatic turning OFF function of LCD
(6) Hard copy function of the screen with a printer in use
Printing of the date on a hard copy is selectable.
(7) External control function
The UVmini can be controlled from an external computer.
The interface used is the RS-232C.
(8) File transfer function
Using optional PC file transfer software, sending data files and condition files to PC and
receiving them from PC is possible.
12
12
12.2
Fixed wavelength measurement, light measurement mode; T% ABS

Quantitation by K factor method (coefficient input)
Saving/loading function of table data
Automatic printing function and automatic output function to the RS-232C port
When the CPS-240 or multi-cell (both options) is used: Sequential measurement up to 6
cells is possible.
UVmini-1240
A
A Optional Accessories
Appendix Optional Accessories
CONTENTS
A-1
A-2
A-3
Sample Module (Multi-cell, Sipper Operation) ................................................................................... A-2

A.1.1 Multi-cell Sample Compartment (6-position)......................................................................... A-2
A.1.2 CPS-240 ............................................................................................................................... A-3
A.1.3 Sipper 160 ............................................................................................................................ A-4
A.1.4 Syringe Sipper ...................................................................................................................... A-5
A.1.5 About the Blank Correction Function .................................................................................... A-6
Printer ................................................................................................................................................ A-8
A.2.1 Installation Procedure ........................................................................................................... A-8
A.2.2 Operating Procedure .......................................................................................................... A-10
List of Cells ...................................................................................................................................... A-11
A
NOTE
Some special accessories may not be used either in the visible model or in the ultraviolet visible model.
For example, DNA & Protein Program can not be used in the visible model, as it is an application for
ultraviolet model. Further, a cell made of glass can not be used in the ultraviolet area.
A
A
UVmini-1240
A-1
A-1
A-1
Sample Module (Multi-cell, Sipper Operation)
When you use a function key to call up the sample control screen from a measurement parameter configuration
screen, you can set the measurement parameters for the sample module.
The following accessories are available which can be mounted in place of the standard sample module and
operated in synchronization with the main instrument.
1. Multi-cell (Cell holder capable of handling 6 cells.)

2. CPS-240 (6-cell holder with a temperature control function)
3. Sipper 160 (Perform measurements while drawing sample into a flow cell using a pump.)
4. Syringe sipper (Perform measurements while drawing sample into a flow cell using a syringe.)
Refer to the instruction manuals of the accessories for the instructions for mounting the various accessories.
Note that 8-position, 12-position, and 16-position cells mentioned in the selection items are not optional.
A.1.1 Multi-cell Sample Compartment (6-position)

This accessory is equipped with a maximum of.6 sample cell holders.
The sample cell holders can be moved automatically or by key operations on the spectrophotometer
unit.
The cell holders at the front is numbered 1.
Display changes
according to selection
of sample module.
Fig. A.1 Sample control screen (6position multi-cell)

(1) Sample module
Specify the name of the instrument mounted in the sample module. Select the 6-position cell.
The screen of Fig. A.1 is displayed after initialization. Set this to 1 when the standard sample
module is used. To initialize the 6-position cell, press [Initial] (F1).
A
A
(2) Drive cell No.

Enter the number of cells being measured.
(3) Reagent blank corr. (cell 1)

This indicates that the sample being used as the reagent blank has been placed in the cell
position 1.
Enter the [3] key to switch the display between "YES" and "NO".
Refer to "A.1.5 About the Blank Correction Function" for details about "reagent blank correction".
A-2
UVmini-1240
A-1 Sample Module (Multi-cell, Sipper Operation)
(4) Cell blank corr.

Set whether or not to perform cell blank correction on the measurement results.
Enter the [4] key to switch the display between "YES" and "NO". If "YES" is set, [Cell BIK](F4)
appears.
Refer to "A.1.5 About the Blank Correction Function" for details about "cell blank correction".
(5) [MoveHome] (F2 key)

This moves cell 1 (the cell inserted into the front-most cell holder) into the measurement light
path.
(6) [MoveCell] (F3 key)
This advances the holder one cell. If cell 6 is the cell which is currently in the light path, the holder
will move to cell 1 when you press this key.
(7) [Cell Blk] (F4 key)
This acquires absorbance (transmittance) data for the cell which is inserted in the respective cell
holder. Cell blank correction subtracts the absorbance acquired at this time from the sample
measurement results for each cell. (The two are divided for transmittance.)
When "Cell blank corr." is set to "YES", be sure to perform cell blank correction for the cell being
used.
This function operates only when "Cell blank corr." is set to "YES".
NOTE
Cells can not be automatically moved and measured in modes which are accompanied by a spectrum
display (Spectrum mode). Press the [MoveCell] (F3) key when you wish to move the cell.
A.1.2 CPS-240
The CPS-240 is an isothermic cell holder which is used to simultaneously hold the 6 cells at a set
temperature through electronic control.
The various settings are the same as for a Multi-cell. Refer to "A.1.1 Multi-cell Sample Compartment
(6-position) ". Refer to the CPS-240 manual regarding setting the temperature of the CPS-240.
A
A
UVmini-1240
A-3
A.1.3 Sipper 160
This is an accessory that uses a pump to draw liquid sample into a flow cell.
Fig. A.2 Sample control screen (Sipper160)

(1) Sample module
When the sample module setting is Sipper 160, a screen like the one above is displayed.
(2) Pump speed
This indicates the pump rotation speed. The input range is 1 to 4: 1. Fast, 2. Medium, 3. Slow, 4.
Halt. Select 4. Halt when using the electromagnetic valve.
(3) Sipping time
Set the time that the sample will be aspirated. The input range is 0 to 99.9 seconds.
(4) Dwell time
Set the time interval between aspiration of a sample and measurement. The input range is 0 to
99.9 seconds.
(5) Purge time

Set the time after measurement is completed that the sample will be purged. The input range is
-99.9 to 99.9 seconds. When set to a negative value, the pump will reverse to purge the sample,
In other words, the sample will be discharged from the tube through which it was aspirated.
(6) No. of rinses
Set the number of times that the inside of the flow cell will be rinsed before measurement. The
sip- purge operation will be repeated the number of times set here. Measurement is not
performed during this operation.
A
A
(7) [Manu.Sip] (F4 key)

This key switches the manual suction function ON/OFF. Each time you press this key, the
function toggles between ON and OFF. Manual sipping draws the sample while the sipper lever is
pressed, regardless of the sipping time setting, and no measurement is performed.
* When using the auto sample changer ASC-5 (optional) for link measurement, refer to the manual for ASC-5.
A-4
UVmini-1240
A.1.4 Syringe Sipper
This is an accessory to sip the liquid sample to the flow cell using the syringe.
Fig. A.3 Sample control screen (syringe sipper)

Initial Initialize
When the unit is connected after turning ON the power, the accessories must be initialized with F1
key. If they have not been initialized, the set capacity of sample may not be sipped or drained.
(1) Sample module
When the sample module is set to "Syringe Sipper," a screen like the one above is displayed.
(2) Pump speed
Select the pump operating speed from four steps: 1.2, 0.6, 0.3, 0.2 or 0.1 ml/sec.
(3) Sip volume
Set the sample sipping volume in the unit of ml. Input range is 0.00 to 10.00 ml.
(4) Dwell time
Set the time from sample sipping to measurement start in the unit of second. Input range is 0.00
to 9.99 sec.
(5) Purge volume

Set the sample purge volume after measurement in the unit of ml. Input range is 0.00 to 10.00 ml.
(6) No. of rinses
Set the number of times that the inside of the flow cell will be rinsed before measurement. The sip
and purge operation will be repeated the number of times set here. Measurement is not
performed during this operation. Input range is 0 to 4 times.
(7) Sample recovery

Select the direction to which the sample is purged after measurement, the drain nozzle side or
the sipping nozzle side.
The [7] key is used to toggle Return (ON) and Discharge (OFF).
UVmini-1240
A-5
A.1.5 About the Blank Correction Function
The blank correction functions included in modes in which a multi-cell or CPS-240 are available in
[SmplCntl] are used in the following instances.
1.
Reagent blank correction

Using the sample placed in cell position 1 as a blank, the cells in the other positions (2-6) are
measured. In other words, after the samples in cell positions 2 through 6 have been measured,
the measured value for the sample in cell position 1 is subtracted from the various measured
values.
Because of this, even if time changes occur in the blank sample or drift develops due to the
increasing temperature of the instrument, accurate data can be acquired by canceling these
fluctuating factors.
2.
Cell blank correction

Even though cells are constructed in the same manner, there are naturally going to be slight
optical differences. In cell blank correction, at first blank samples are placed in a square cells and
the measured values in that condition are recorded. Then, when an unknown sample is
measured and those results are displayed, the previously recorded blank measured value is
canceled from the various measurement results. Thus, the measured value of only the sample is
obtained.
If both of these two blank correction functions are functioning, the measured value for the other cells
can be corrected with the sample in cell 1 as the reagent blank, while also performing a cell blank for
each individual cell.
Blank correction is one type of data processing and is calculated as subtraction for absorbance and
division for transmittance.
Note that the standard measurement modes in which the blank correction function can be used are
the Photometric mode and Quantitation mode. This function can not be used in the Spectrum mode.
Table A.1 Blank Correction List
ABS
Cell 1
(Cell used
as reagent
blank)
An
An-A1
An-an
(An-an)-(A1-a1)
T%
Output Data
Cell n
(n=2-6)
Tn
Tn/T1 100(%)
Tn/tn 100(%)
Tn.t1/tn.T1 100(%)
ABS
Cell blank corr.=NO Cell blank corr.=NO Cell blank corr.=YES Cell blank corr.=YES
Reag blank corr.=NO Reag blank corr.=YES Reag blank corr.=NO Reag blank corr.=YES
A1
Photometric: A1
Photometric: A1-a1
A1-a1
Photometric: A1-a1
Photometric: (A1-a)-(A1-a1)
T%
Cell
Position
T1
T1
T1/t1 100(%)
T1/t1 100(%)
A
Wherein,
An: Absorbance with no blank correction on cell n
Tn: Transmittance with no blank correction on cell n
an: Cell blank value for cell n (absorbance)
tn: Cell blank value for cell n (transmittance)
A-6
UVmini-1240
NOTE
The range over which correction can be performed, due to internal data processing calculation
restrictions, are up to 399.9% for transmittance and down to -0.602Abs for absorbance.
When a blank correction function is working, accurate data are obtained only when an actual
measurement operation has been performed (when the [START/STOP] key has been pressed).
Please note that the value displayed in the upper right corner of the sample control screen or
measuring mode, which indicates the current value, displays the uncorrected value.
A
A
UVmini-1240
A-7
A-2
A
A-2
Printer
NOTE
Refer to the printer's user's manual for detailed operation of the printer.
This section explains how to connect the printer to the UVmini-1240 using thermal printer the HCP-1C as an
example. The explanation given here is also applicable to the thermal printer DPU-414 or the printers which
conform(s) to ESC/P except selection items in step 7.
Fig. A.4 Thermal printer
A.2.1 Installation Procedure

The HCP-1C and the UVmini-1240 are connected using the printer cable included with the printer. For
connecting a ESC/P type printer and a PCL3 type printer, the special printer cable (088-50904-20) is
required.
Table A.2 Printer Installation Procedure
Step
1

Return to the Top Menu.
Key Operation
Press the [RETURN] key until the
screen stops changing.
A
A
A
* continued
A-8
UVmini-1240
A-2 Printer
Step
3
Key Operation
Select "5. Utilities"
[5]
Select "7. Printer"
[7]
Select "HCP"
5
6
] or [
NOTE
Turn on the printer switch after turning ON the spectrophotometer switch. And turned OFF the
spectrophotometer switch after checking the Printer switch is turned OFF.
If not operating in a proper order mentioned above, the printer prints out irregular characters continuously
while the Printer switch is ON.
A
A
UVmini-1240
A-9
A-2 Printer
A.2.2 Operating Procedure
(1) Hard copy of screen : A hard copy of the screen will be printed when you press the [PRINT] key.
(2) Print using function key: When the function keys are assigned a [PRINT] function as in modes in
which the measurement results are recorded in table format, as in photometrics, all of the table
data will be printed.
(3) Print numeric data for each measurement: When the measurement results are obtained in the
form of numeric values, as in photometrics, the measurement results will be automatically printed
if the printer is simply connected to the unit.
(4) To feed paper: Press the [ON LINE] key on the printer to turn the on line display OFF. Then press
the [FEED] key.
After using this, press the [ON LINE] key again to turn the on line display back ON.
A
A
A-10
UVmini-1240
A-3
A-3
List of Cells
Table A.3 List of Optional Cells
Name
Shape Quartz (S cell) Glass (G cell)
A
Q'ty
Special holder
Square cell, optical length 10 mm
200-34442
200-34565
Not required
Square cell, matching type
201-98716
201-98746
2/set
Not required
Sealed-type square cell, optical

length 10 mm
200-34444
200-34444-01
Not required
Semi microcell, optical length

10 mm required sample volume
1.0 ml or more
200-66501
200-66501-01
Not required
Semi microcell, optical length

10 mm required sample volume
1.0 ml or more
200-66551
Not required
Super-micro black cell, with 10 mm

optical path and required sample
volume of 100 L or more
200-66578-11
Super-micro cell
holder (206-1433401) required
Micro black cell, with 10 mm optical

path and required sample volume
of 100 mL more
200-66578-12
Super-micro cell
holder (206-1433401) required
Cylindrical cell
(OD 25") (ID
22")
200-34448
200-34448-01
(quartz window) (glass window)
Cylindrical cell holder

(204-06216) required
200-34472
200-34472-01
200-34473-01 200-34473-03
200-34473-02 200-34473-04
L(Optical.length)
= 10 mm
L = 20 mm
L = 50 mm
L = 100 mm
Square long
absorption cell
L = 20 mm
Short optical
length cell
L = 1 mm
200-34446
200-34446-01
200-34944
200-34944-01
200-34660-01
200-34662-01
L = 2 mm
200-34655
200-34662-11
L = 5 mm
200-34449
200-34449-01
L = 50 mm
H
Spacer for short for 1 mm

optical length cell
for 2 mm
for 5 mm
204-21473-03
204-21473-01
204-21473-02
Long-path rectangular
cell holder (20423118-01) required
Short optical length
cell spacer required
Not required
Optical
length
Shape
L= 10 mm
200-34670
1.5 mL
4 36
Not required,
but front plate
with hole
required
For general
use, without
tubes
Flow cell for

L= 10 mm
Syringe Sipper
220-92618
33 L
2 10
Not required
include tubes
Nomenclature
Flow cell
Quartz cell
Optical
Capacity
(S cell)
width of cell
Special
holder
UVmini-1240
Remarks
A-11
A-3 List of Cells
unit: mm
Fig. A.5 Optional Cell Shapes
A
A
A-12
UVmini-1240
A-3 List of Cells
Table A.4 Record of Revision

Date
Revision
Changed Page
Description
1/2001
Regulatory Information
CE
2/2004
Regulatory Information,
3-12, 8-4, 9-5, 9-7,
Appendix-10, Appendix-11
Record of Revision
Clerical error correction.

IVDD.
6/2005
1-2, 3-6, 3-13, 4-5, 5-6, 8-2,

8-3, 8-5, 11-12, 11-13,
Appendix A-8, A-9
PCL printer
1/2006
A: Action for Environment
9/2006
Modification in layout
Modification in phrases,
pointed out by a native
checker
A
A
Note) A ...... Added Page No.

D ...... deleted Page No.
UVmini-1240
A-13
A-3 List of Cells
A
A
A-14
UVmini-1240

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206-94623E

Shimadzu UV Visible Spectrophotometer

Read the instruction manual thoroughly before you use the

Precautions for Safe Operation

MEANING OF CAUTION SIGNS

Replacement Parts Availability

Warning Labels Indicated on the Unit

: UV-Visible Spectrophotometer/Visible Spectrophotometer

Authorized Representative in EU: Shimadzu Europa GmbH

: Albert-Hahn-Strasse 6-10, 47269 Duisburg, F. R. Germany

Action for Environment

Parts Inspection ................................................................................................................................. 1-2

Exterior View ..................................................................................................................................... 2-2

Chapter 3 Mode Selection and Shared Operations

Mode Selection Screen ..................................................................................................................... 3-2

Chapter 4 Photometric Mode

Measurement Parameter Configuration Screen ................................................................................ 4-2

Chapter 5 Spectrum Mode

Measurement Parameter Configuration Screen ................................................................................ 5-2

Chapter 6 Quantitation Mode

Quantitation Mode ............................................................................................................................. 6-2

Chapter 7 Optional Program Pack

Starting the Optional Program Pack .................................................................................................. 7-2

Chapter 8 Utilities Mode

Utilities Screen................................................................................................................................... 8-2

Connecting to a PC............................................................................................................................ 9-2

Programming Example ...................................................................................................................... 9-5

Chapter 10 File Transfer

Chapter 11 Maintenance & Checking

Daily Maintenance & Periodic Maintenance .................................................................................... 11-2

Appendix Optional Accessories

Sample Module (Multi-cell, Sipper Operation) ................................................................................... A-2

This page is intentionally left blank.

Chapter 1 Before Using

Parts Inspection ................................................................................................................................. 1-2

Table 1.1 Standard Contents

Spectrophotometer (One of the following)

Standard Accessories (One of the following)

(for 100V to 120V)

Fig. 1.1 Standard Contents

Room temperature during use of 15 to 35C.

1.2.2 Cleaning the Unit

1.3.1 Power Supply

1.3.3 Connecting the Power Cable

(5) Turn ON the unit power switch.

The fuses are mounted inside.

Fig. 1.2 Power Supply Connector

Turning ON Power and Initialization

Fig. 1.3 Initialization screen

Installation Function Check

1.5.1 Baseline Flatness

Table 1.2 Procedure (Baseline Flatness)

Operation, Monitor Display

Turn ON power (Let sit for 60 minutes after automatic

Select "2. Spectrum"

Set the measurement mode to "ABS"

Enter [1] until "ABS" is displayed.

Set the scan range to 1100 ~ 200 nm

Set the photometric range to -0.01 ~ 0.01Abs.

Set the scan speed to "Fast".

1.5 Installation Function Check

Operation, Monitor Display