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GC Detectors
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as there is interactive material that cannot be fully shown in this reference
manual.
Objectives
Content
Overview of GC Detectors
Concentration versus Mass Flow
Selective versus Universal
Destructive versus Nondestructive
Characteristics of GC Detectors
Noise
Signal to Noise Ratio
The Flame Ionisation Detector
Overview
Operating and Optimising
Uses and Performance
The Nitrogen Phosphorous Detector (NPD)
Overview
Operating and Optimising
Use and Performance
The Electron Capture Detector (ECD)
Overview
Operating and Optimising
Uses and Performance
The Thermal Conductivity Detector (TCD)
Overview
Operating and Optimising
Uses and Performance
Other GC Detectors
Flame Photometric Detector (FPD)
Photoionisation Detector (PID)
Electrolytic Conductivity Detector (ELCD)
Mass Spectrometer (MS)
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Overview of GC Detectors
There have been over 60 detectors designed for GC applications since the inception of
the technique. Probably 10-12 detectors are used commonly in routine GC applications
and of these only 3-4 are used in non-specific applications.
All GC detectors monitor a physical property of the analyte the property chosen must
significantly change, and therefore cause a large detector response, when the eluent gas
flowing into the detector is contaminated by the analyte. Several properties have been
used in GC detectors and these are outlined in the next table. Detectors that exhibit an
enhanced response to certain analyte types are known as selective detectors.
Table 1. Selected GC detectors
Detector type
Detector (Property)
Ionisation
Flame Ionisation Detector (FID)
Thermal Conductivity Detector (TCD)
Electron Capture Detector
Nitrogen Phosphorous Detector (NPD)
Photoionisation Detector (PID)
Emission
Flame Photometric Detector (FPD)
Plasma Atomic Emission (AED)
Electrochemical
Hall Electrolytic Conductivity (HECD)
Others
Chemiluminescent
Mass Spectrometer (MSD)
Fourier Transform Infra-red (FTIR)
Selective
No
No
Halogens
N, P, Halogens
Aromatics
S, P
Metal, Halogen, C, O
S, N, Halogen
S
Yes
Yes
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MS in Selected Ion mode responds to only compounds producing certain fragment ions.
The chromatogram is simplified compared to the top example due to the specificity of the
detection mode.
Destructive versus Nondestructive
Nondestructive detectors are required where the separated intact analyte components
need to be recovered for further analysis or characterisation. This can be accomplished
using destructive detectors (where the analyte is destroyed) by splitting the carrier effluent
stream just prior to the detector entrance.
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Characteristics of GC Detectors
There are several important detector characteristics that apply to all Gas Chromatography
detectors and help to describe the detector performance. The important characteristics of
GC detectors include:
These characteristics will be explored further using some of the terms outlined in the brief
definitions above.
Noise
Noise is the background signal produced by the detector in the absence of analyte. It
arises from electronic noise, stray environmental signals and contamination or leaks.
Good design and shielding can help to reduce the inherent noise. Noise is a rapid
random variation in output, whereas drift is a slow systematic change in detector output.
Signal to Noise Ratio
The ratio of the detector signal to the inherent background noise is a useful measure of
detector performance to convey information about the lower limit of detection.
Conventionally the lowest signal to noise ratio that can be attributed to an analyte would
have a signal to noise (S/N) ratio of 2. From the figure opposite showing a S/N value of 2,
the signal is only just perceptible from the background noise. Different regulatory
authorities in various industries may specify limit of detection as a signal to noise value
the actual value will vary from authority to authority but will usually be either 2 or 3.
Schematic representation of the determination of detector (FID) noise using the ASTM
method
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As the column effluent is burned in this flame, ions are created which form a small current
when a potential difference is applied. When no analyte (carbon containing compounds)
are being burned, a small background current (10-20 picoamperes) arises from impurities
in the carrier and detector gases. Conventionally the jet forms the anode and a cylindrical
electrode held just above the flame is the cathode. A voltage of between 200 and 300V
across these components is usually optimal but depends on detector design. The exact
mechanism of ion production is not well characterised, however the formation of carbon
ions via pyrolysis and the formation of small organic fragment ions produced via high
energy combustion products are popular theories. The flame ionisation detector produces
a proportional response to the number of carbon atoms in a molecule. One suggested
reason for this constant response factor is the conversion of all solute carbon molecules to
methane in the combustion process. When heteroatoms are present within the analyte
molecule the sensitivity of the detector is much reduced. A calibration curve should be
constructed for each analyte prior to quantitative analysis to take into account response
variations due to detector settings.
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Once past a critical minimum value the actual flow of the oxidiser seems to make little
difference to the detector sensitivity. Typically this value will be between 300 and
400mL/min. The critical ratio for sustaining a flame is 8-12% hydrogen in air.
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The presence of water in samples often produces very large tailing solvent peaks and
induces tailing in analyte peaks. Having a detector that effectively ignores the water is
sometimes useful at simplifying the chromatogram. Other solvents (such as carbon
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disulphide) are also ignored by the detector and have utility when dealing with very early
eluting analytes that may otherwise elute beneath the solvent peak.
The general uses of FID are too widespread to categorise fully here open any column
manufacturers catalogue to find a host of relevant applications. A few typical examples
have been shown cited opposite for general reference.
The FID performance figures of merit are shownhighlighting just why this detector is the
most popular GC detector in use today. Its excellent sensitivity, linearity and wide
applicability make it the foremost detector in general organic analysis.
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Where:
1. Methanol
2. Acetaldehyde
3. Ethanol
Headspace conditions.
Oven:
70oC
Loop:
80oC
Transfer line:
90oC
Vial equil time:
10oC
Press time:
0.20min
4. Isopropanol
5. Acetone
6. 1-Propanol
0.20min
0.05min
0.1-0.20min
1.0mL
0.1% ethanol
Analysis conditions.
Carrier
Oven
Injector
Detector
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Nitrogen/Phosphorous containing Pesticides (carrier gas 30cm/sec He), EPA method 507
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Where:
1. Prometon
2. Atrazine
3. Prometryne
4. Ametryne
Detector
Temperature:
Makeup gas:
Hydrogen:
290oC
He, 30mL/min
4mL/min
Injection parameters
Temp:
Vol injected:
Pneumatics:
Purge delay:
250oC
1L
Splitless mode
1min
Determination of carbaryl
Conditions.
Injection parameters
Pressure programming
Oven:
70oC (1min) to 230oC
(5min) at 20oC/min
Column:
HP-5,
300.32mm0.25mmL
N2, 15mL/min
Makeup gas:
Column flow:
2mL/min
Detector temp:
330oC
Detector: 3mL/min of H2, air at 60mL/min
Inlet: SL mode, 60mL/min purge 0.75min
Inlet temp:
250oC
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Propazine
Atrazine
Simazine
Terbuthylazine
5.
6.
7.
8.
Prometryne
Ametryne
Simetryne
Terbutryne
Detector:
NPD 250oC
Makeup gas:
He at 28mL/min
Injector:
Split 1:10, 250oC
Column: DB-35 30m0.25mm0.25m
Ni
These negatively charged particles collide with carrier gas molecules and produce further,
higher energy, electrons (remember that beta particles are themselves low energy
electrons).
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+ N 2 2e + N 2+
The electrons formed by this process establish a high standing current between the anode
(usually the inlet tube or detector body) and the cathode (usually a cylindrical electrode in
the centre or top of the detector). The potential difference applied is usually 20-100V dc).
When an electronegative analyte elutes, the analyte molecules capture some of the
background electrons and this results in a reduction of the standing (background)
current.
A + e A
The negative analyte ions formed are slow moving and are not collected by the anode.
The extent of electron absorption, and hence the reduction in standing current, is
proportional to the concentration of the analyte.
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Organochloride pesticides
Where:
1. Trifluralin
2. Chloroneb
3. Propachlor
4. HCB
5. -BHC
6. r-BHC
7. -BHC
8. Chlorothalonil
9. Heptachlor
Conditions.
Oven
Initial temp:
1st ramp:
Dwell:
2nd ramp:
Final temp:
10. -BHC
11. Alachlor
12. Aldrin
13. DCPA
14. Heptachlor
epoxide
15. r-Chlordane
16. o,p-DDE
17. -Chlordane
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18.
19.
20.
21.
22.
23.
24.
25.
26.
Endosulfan I
p,p-DDE
Dieldrin
o,p-DDD
Chlorobenzilate
Endrin
o,p-DDT
p,p-DDD
Endosulfan II
27.p,p-DDT
28.Endrin
aldehyde
29.Endosulfan
sulphate
30.Dibuthyl
chlorendate
31.Methoxychlor
32.HBB
Detector:
ECD 325oC
Makeup gas:
N2 at 30mL/min
Carrier:
He at 35cm/sec measured, 250oC
Column:
DB-5 30m0.53mm1.5m
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Where:
1. Chloroacetic acid
2. Bromoacetic acid
3. Dichloroacetic acid
4. Dalapon
5. Trichloroacetic acid
6. Bromochloroacetic acid
Conditions.
Oven
Initial temp:
1st ramp:
Dwell:
2nd ramp:
Dwell:
3rd ramp:
Final temp:
35 C for 10min
to 75oC at 5oC/min
75oC for 15 min
to 100oC at 5oC/min
100oC for 5 min
to 135oC at 5oC/min
135oC for 5min
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7. 1,2,3,-Trichloropropane (ISTD)
8. Dibromoacetic acid
9. Bromodichloroacetic acid
10. Chlorodibromodichloroacetic acid
11. 2,3,-Dibromopropionic acid (SSTD)
12. Tribromoacetic acid
Detector:
ECD 260oC
Makeup gas:
N2 at 30mL/min
Injector:
Splitless, 200oC, 30sec purge
Column:
DB-1701 30m0.25mm0.25m
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Fluorotrichloromethane
Methylene chloride
trans-1,2-Dichloroethylene
1,1-Dichloroethylene
2,2- Dichloroethane, cis-1,2
Dichloroethylene
6. Bromochloromethane
7. Chloroform
8. 1,1,1-Trichloroethane
9. Carbon tetrachloride
10. 1,2-Dichloroethylene
11. 1,2-Dichloropropane
Conditions.
Oven
Initial temp:
35oC for 5min
Ramp:
to 135oC at 4oC/min
12. Bromodichloromethane
13. 1,1,2-Trichloroethane, 1,3dichloropropane, tetrachloroethylene
14. Dibromochloromethane
15. 1,2-Dibromoethane (ECB)
16. 1,1,1,2-Tetrachloroethane
17. Bromoform
18.1,2,3-Trichloropropane, 1,1,2,2tetrachloroethane
19. Pentachloroethane
20. bis-(2-chloroisopropyl) ether
21. 1,2-Dibromo-3-chloropropane
Detector:
ECD 300oC
Makeup gas:
N2 at 40mL/min
Injector: Purge and trap (desorption 8mL/min)
Column:
DB-624 30m0.53mm3.0m
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When an analyte elutes into the analytical cell the thermal conductivity of the gas
changes which causes a change in the rate of heat loss from the analytical filament. The
Wheatstone Bridge becomes unbalanced and current needs to be supplied in order to
restore the balance.
Whilst the gas flowing over the two filaments is equal the thermal conductivity will be
equal and the filaments will have the same rate of heat loss the Wheatsone bridge will be
balanced and the signal will be zero. If an analyte elutes with the carrier, the thermal
conductivity of the gas in the analysis cell changes, the rates of heat loss of the two
filaments will not be balanced and a current will have to be applied to balance the
Wheatstone bridge. It is this current which is recorded as the signal. The size of the
applied current will be related to the concentration of analyte in the effluent gas. The size
of the cell is critical in determining the sensitivity of the detector with 140 L being typical
in a detector used for packed column GC. These devices cannot be used with narrow
bore capillary columns and the cell volume must be drastically reduced. Several
manufacturers offer single cell devices of very low volume (~5L) for use with capillary
columns in which the reference and analysis streams are switched onto the single filament
in the order of 5-10Hz. Ultra-small detector devices etched onto silica wafers are also
available but are not used routinely.
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Thermal conductivity detector. In order to reduce volume single cell TCDs are used with
capillary columns this design has a cell volume of around 5L. The switching occurs
around to times/second
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Oven
Initial temp:
50oC for 10min
Ramp:
to 120oC at 10oC/min
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4. Methane
5. Carbon monoxide
Injector:
Detector:
Makeup:
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Where:
1.
2.
3.
4.
Nitrogen
CO2
SF6
COS
5. H2S
6. Ethylene oxide
7. SO2
Conditions.
Carrier gas:
Helium at 53 cm/sec
Oven:
25oC for 3 min
o
o
25-200 C (10 C/min) -200oC hold
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Methane
Ethylene
Ethane
Propylene
Propane
Isobutane
Conditions.
Carrier gas:
Oven:
7. 1-Butene
8. n-Butane
9. cis-2-Butene
10. trans-2-Butene
11. Isopentane
12. n-Pentane
Helium at 8 mL/min
70oC for 3 min
70-180oC (20oC/min)
180oC hold for 3 min
180-230oC (5oC/min)
230oC hold for 10 min
Injector:
Detector:
13. n-Hexane
14. n-Heptane
15. n-Octane
16. n-Nonane
17. n-Decane
Other GC Detectors
Flame Photometric Detector (FPD)
Compounds are burned in a hydrogen-air flame very similar to an FID detector. Sulphur
and phosphorous containing compounds produce light emitting species (sulphur 394nm
/ phosphorous 526nm). A monochromatic filter allows only one of the wavelengths to
pass and a photomultiplier tube is used to measure the amount of incident light and a
signal is generated. A different filter is required for each detection mode.
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Photoionisation Detector
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Mass Spectrometer
Mass Spectrometer Characteristics
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