Академический Документы
Профессиональный Документы
Культура Документы
Ph ton
Abstract
Introduction: The microbial quality of drinking water is
of utmost importance and can be defined using
microbiological tools, indicator organisms. These
organisms show the possibility of pollution of water with
pathogenic organism excreted in faeces. The presence of
1. Introduction
Safe drinking water remains inaccessible to several
millions people in the globe. Contamination of
drinking water due to natural and manmade
contaminants is frequently reported in developing
countries, where mainstream of the inhabitants
survive in countryside and uptown areas with
meager
hygiene
and
waste
clearance
practices (Jensen P.K., 2002). Opportunistic
pathogen infections are a serious public health
problem, especially to debilitated persons, in any
areas where large numbers of people are in close
confinement, particularly hospitals and senior care
facilities. At least five percent of patients admitted
to hospitals acquire nosocomial infections and
about one percent of the affected patients die as a
direct result. Many of these organisms are found
Ph ton
supply
295
Location
1
2
3
4
5
6
7
8
9
10
11
12
13
296
4. Results
Table 2: Percentage of the various types of organisms isolated form water samples
ORGANISM
Escherichia coli
Citrobacter species
Acinetobacter species
Pseudomonas species
Gram negative non-fermentor
Sterile
1%
9%
9%
4%
3%
74%
1
9
9
4
3
74
Ph ton
result
297
5. Discussion
MPN (Most Probable Number) studies take into
consideration the isolation of Escherichia coli,
Klebsiella species, Enterobacter species and
Citrobacter species as significant in relation to
fitness for drinking purpose. Other organisms are
considered as saprophytic contamination.
In the study on water samples from water taps,
from various sources, it was seen that Boys hostel
mess, Maxillofacial ward, Physiotherapy canteen,
Microbiology department water samples did not
show any bacterial growth any time meaning good
quality of water. In the Engineering canteen
sample, 5 tap water samples were studied.
Organisms isolated were Citrobacter species at
both times with MPN count of greater than 10 per
100 ml of water indicating unsatisfactory for
consumption. Tap water samples were studied from
Casualty canteen water. Sample in month of June
showed growth of GNNF and sample of August
showed growth of E.coli and GNNF. The MPN
count of E. coli isolated was more than 10 per
100ml of water. Thus was proved unsatisfactory for
consumption.
Water samples from water coolers too showed a
similar picture as that of the tap water.
In this study done with100 samples we can observe
that 10% were coliforms, 16% were non coliforms
and the remaining 74% were sterile.
Of the 10% coliforms 9% were Citrobacter species
and 1% was E. coli. These samples indicate that
water at the monsoon time was not suitable for
drinking purpose and proper corrective measures
were advised to the concerned authority.
Ph ton
298
Acknowledgement
Recommendations
Portable water quality is necessary to be monitored
by conducting bacteriological analysis in a routine
manner. This is of even more necessicity in health
care setups. The chances of post operative
infections transmitted by water can be controlled
by this. A simple method of plate count, followed
by bacterial identification can be adopted for this.
Authors Contribution and Competing Interests
1. Minakshi Bhattacharjee- Research worker.
2. Dr. A.D. Urhekar- Guidance and intellectual
inputs.
3. Dr. Revati Sharma- Guidance and results
interpretation.
Ph ton
299