Академический Документы
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Культура Документы
1. Introduction
2. Stationary phases
3. Retention in Gas-Liquid Chromatography
4. Capillary gas-chromatography
5. Sample preparation and injection
6. Detectors
(Chapter 2 and 3 in The essence of chromatography)
A. General descriptors
(1) tR, tR, and tM
(2) VR, VR, and VM
(3) LR, LR, and LM
3
j=
2
(Pi/P0)2-1
(Pi/P0)3-1
(4) VR = tR * F, and LR = tR * u
(5) V = t * F, and L = t * u
(6) k = tR/ tM =
(7) K = k* = k*
VM
VS
(8) tR = tM(1+k)
(9) = k1/k2
(10) Rs = (tR2-tR1)/[(Wb1+Wb2)/2] = [N1/2/4][( -1)/()]*[k2/(1+ k2)],
H
s2 +
H
a2 +
16
I = 100z +100*[logtR(x)-logtR(z)]/[logtR(z+1)-logtR(z)]
Squalene (C30H62)
Idea is same: use constants from systems and solute to describe retention
Difference: Kamlet et al use solvatochromic parameters to index the
constant of solute of interest.
McReynolds uses properties of specific molecules to index
constant of solute of interest.
Method by McReynolds
McReynolds phase constants
I = aX +bY + cZ + dU +eS
Phase constant: X: Benzene; Y: 1-butanol; Z: 2-pentanone; U: 1nitropropane; S: Pyridine
a, b, c, d, e, constants for the solute of interest.
16
= k1/k2 = K1/K2
2. Coupled columns
Column A
Column B
Ks = A KA + B KB
Ks = (PKA + KB) / (P+1)
P = tMA/tMB
2. Flow programming:
Htot = A + B/u + Cu