Compliance of manufacturers of

AST materials and devices with

EUCAST guidelines
Data are based on questionnaires to manufacturers of materials and
devices for antimicrobial susceptibility testing.
The tables will be updated when manufacturers report changes
(contact erika.matuschek@escmid.org).
The accuracy of data in these tables is not verified by EUCAST and
the inclusion of any materials or devices does not indicate
endorsement by EUCAST.
Last updated 10 March 2016

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

EUCAST disk diffusion method

Manufacturer

Disks available

MH-F plates available

Abtek

All except ceftobiprole 5 g and

ceftolozane-tazobactam 30-10 g

No

BD

All except ceftobiprole 5 g and

ceftolozane-tazobactam 30-10 g

Expected 2016

All except ceftaroline 5 g, ceftobiprole 5

g and ceftolozane-tazobactam 30-10 g

No

All except ceftobiprole 5 g

Yes

No disks marketed

Yes

All except ceftaroline 5 g

and ceftobiprole 5 g

No

All except ceftolozane-tazobactam

30-10 g (expected April 2016)

Yes

All except ceftobiprole 5 g

No but supply -NAD supplement

All except ceftobiprole 5 g,

ceftolozane-tazobactam 30-10 g and
ticarcillin-clavulanic acid 75-10 g

Yes

All (Neo-Sensitabs)

No

Bioanalyse
Bio-Rad
bioMrieux

I2a
Liofilchem
MAST Group
Thermo Fisher Scientific (Oxoid)

Rosco

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Automated systems for reading antimicrobial

susceptibility testing plates
Manufacturer

System

EUCAST
breakpoints
implemented

EUCAST Expert
Rules
implemented

EUCAST terminology
implemented
S

R>

IE

Yes

Yes

Yes

Yes

Bio-Rad

OSIRIS/ADAGIO

Yes

Yes.
There are additional
rules but EUCAST
rules have priority.

Giles Scientific

BIOMIC V3

Yes

Yes.
No exceptions

Yes

Yes

Yes

Yes

I2a

SIRSCAN:
2000
2000 automatic
Micro

Yes

Yes.
There are additional
rules but none
should be contrary
to EUCAST rules.

Yes

Yes

Yes

Yes

Yes

Yes.
EUCAST rule
number displayed.

Yes

Yes

No

Synbiosis Division of
Synoptics

ChromaZona

No

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Gradient tests: Etest (bioMrieux)

Manufacturer
bioMrieux

Agents in EUCAST tables but

not available
Amoxicillin-clavulanic acid (fixed
2 mg/L clavulanic acid)*, **
Ampicillin-sulbactam (fixed 4
mg/L sulbactam)*, **
Cefadroxil
Cefalexin
Cefazolin
Ceftibuten
Ceftobiprole
(available for resaerch use only)
Ceftolozane-tazobactam
(expected 2016)
Oritavancin
Roxithromycin
Tedizolid
Ticarcillin

Agents validated for MH-F

Ampicillin
Amoxicillin
Benzylpenicillin
Cefaclor
Cefepime
Cefixime
Cefotaxime
Cefpodoxime
Ceftriaxone
Cefuroxime
Doripenem
Ertapenem
Imipenem
Meropenem
Chloramphenicol
Ciprofloxacin
Levofloxacin
Moxifloxacin
Ofloxacin

Azithromycin
Clarithromycin
Erythromycin
Clindamycin
Doxycycline
Minocycline
Tetracycline
Tigecycline
Teicoplanin
Vancomycin
Daptomycin
Linezolid
Nitofurantoin
Rifampicin
Trimethoprim
Trimethoprim-sulfamethoxazole
(not for H. influenzae and M. catarrhalis)

*Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
** Expected during 2014

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Gradient tests: MIC Test Strip (Liofilchem)

Manufacturer
Liofilchem

Agents in EUCAST tables

but not available
Cefadroxil
Cefalexin
Roxithromycin
Telithromycin
Ticarcillin

Agents validated for MH-F

Amoxicillin
Amoxicillin-clavulanic acid
(fixed 2 mg/L clavulanic acid)*
Ampicillin
Ampicillin-sulbactam
(fixed 4 mg/L sulbactam)*
Azithromycin
Benzylpenicillin
Cefaclor
Cefepime
Cefixime
Cefotaxime
Cefpodoxime
Ceftaroline
Ceftriaxone
Cefuroxime
Cephalothin
Chloramphenicol
Ciprofloxacin

Clarithromycin
Clindamycin
Doripenem
Doxycycline
Ertapenem
Erythromycin
Imipenem
Levofloxacin
Linezolid
Meropenem
Minocycline
Moxifloxacin
Ofloxacin
Rifampicin
Teicoplanin
Tetracycline
Trimethoprim-sulfamethoxazole
Vancomycin

*Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Gradient tests: M.I.C.Evaluator

(Thermo Fisher Scientific)
Manufacturer

Agents in EUCAST tables but not available

Agents validated for MH-F

Thermo Fisher
Scientific (Oxoid)

Amoxicillin-clavulanic acid
(fixed 2 mg/L clavulanic acid)*
Ampicillin-sulbactam
(fixed 4 mg/L sulbactam)*
Azithromycin
Aztreonam
Cefaclor
Cefadroxil
Cefalexin
Cefazolin
Cefepime
Cefixime
Cefoxitin
Cefpodoxime
Ceftibuten
Ceftobiprole
Ceftolozane-tazobactam
Cefuroxime
Chloramphenicol
Clarithromycin
Colistin
Dalbavancin
Doripenem
Doxycycline
Ertapenem
Fosfomycin

Amoxicillin
Ampicillin
Benzylpenicillin
Oxacillin
Cefotaxime
Ceftazidime
Ceftaroline
Levofloxacin
Amikacin
Teicoplanin
Vancomycin
Erythromycin
Tetracycline
Tigecycline
Linezolid

Fusidic acid
Mecillinam
Minocycline
Moxifloxacin
Mupirocin
Nalidixic acid
Netilmicin
Nitrofurantoin
Norfloxacin
Ofloxacin
Oitavancin
Piperacillin
Piperacillin-tazobactam
Quinupristin-dalfopristin
Rifampicin
Roxithromycin
Spectinomycin
Streptomycin
Tedizolid
Telavancin
Telithromycin
Ticarcillin
Ticarcillin-clavulanate
Tobramycin
Trimethoprim
Trimethoprim-sulfamethoxazole

*Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Phoenix/EpiCenter automated system (BD)

In computer
database
EUCAST terminology
implemented
In reports

Yes

R>

No (R )

Yes

IE

Yes

Yes

R>

Yes ( converted to R >)

Yes (MICs reported for agents with no EUCAST breakpoints)

IE

Yes (MICs reported for agents with no EUCAST breakpoints)

EUCAST Expert Rules

implemented

All rules incorporated. There are additional rules but EUCAST rules have priority.

EUCAST organism groups

with no test in the system

H. influenzae
M. catarrhalis

Agents in EUCAST tables

but not available in the
system

Ampicillin-sulbactam (fixed 4 mg/l sulbactam)*

Ceftobiprole
Ceftolozane-tazobactam

Agents available but

EUCAST breakpoints not
implemented in the system

Rifampicin (Staphylococcus spp. cannot be reported susceptible)

Trimethoprim (Enterococcus spp. cannot be reported susceptible)
Trimethoprim-sulfamethoxazole (Enterococcus spp. cannot be reported susceptible)

N. meningitidis
N. gonorrhoeae

Gram-negative anaerobes
Gram-positive anaerobes

*Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

MicroScan automated system

(Beckman Coultier Diagnostics)
In computer
database
EUCAST terminology
implemented
In reports

Yes

R>

Yes

No

IE

No

Yes

R>

Yes

Most - do not have interpretations reported

IE

No

EUCAST Expert Rules

implemented

Yes. There are other rules in the system but EUCAST rules take precedence for EUCAST
panels. All rules can be customized by user.

EUCAST organism groups

with no test in the system

M. catarrhalis
Pasteurella

N. meningitidis
N. gonorrhoeae

Agents in EUCAST tables

but not available in the
system

Doxycycline
Roxithromycin
Telithromycin
Dalbavancin
Oritavancin
Tedizolid

Ampicillin-sulbactam (fixed 4 mg/l sulbactam)*

Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)*
Ceftobiprole
Ceftolozane-tazobactam
All agents for anaerobes

Agents available but

EUCAST breakpoints not
implemented in the system

Chloramphenicol
Fusidic acid
Cefuroxime

Gram-negative anaerobes
Gram-positive anaerobes

Tigecycline (Gram-positive organisms)

Rifampicin
Trimethoprim

*Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

VITEK 2 automated system (bioMrieux)

EUCAST terminology
implemented

EUCAST Expert Rules

implemented

S
Yes
R>
Yes
In computer database
Yes
IE
Yes
S
Yes
R>
Yes
In reports
Yes
IE
Yes
All applicable rules dealing with intrinsic resistance, therapeutic interpretation and deduction are implemented beginning with
software version V2S 5.03 (2011) . There are additional rules but none should be contrary to EUCAST rules in this software version.

H. influenzae
Gram-negative anaerobes
M. catarrhalis
Gram-positive anaerobes
N. meningitidis
H. pylori
N. gonorrhoeae
L. monocytogenes
Azithromycin
Roxithromycin
Ampicillin-sulbactam (fixed 4 mg/L sulbactam) *
(Staphylococcus spp. and Enterococcus spp.)
Phenoxymethylpenicillin
Agents in EUCAST tables but not
Cefadroxil
available in the system
Ceftobiprole
Ceftibuten
Ceftolozane-tazobactam (expected during 2016)
Cefepime (S. pneumoniae)
Cefpodoxime (S. pneumoniae)
Netilmicin (Staphylococcus spp.)
Trimethoprim (Enterococcus spp. and group B streptococci)
Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)*
Agents available but EUCAST
uncomplicated UTI breakpoints (Enterobacteriaceae)
breakpoints not implemented in Doripenem (Enterobacteriaceae, Pseudomonas spp.,
the system
Acinetobacter spp.)
Ciprofloxacin (Salmonella spp. and Enterococcus spp.)
Levofloxacin (Enterococcus spp.)
Norfloxacin (Enterococcus spp.)
EUCAST organism groups with
no test in the system

C. jejuni and coli

Corynebacterium spp.
M. tuberculosis
Cefuroxime (S. pneumoniae )
Dalbavancin
Daptomycin
Doxycycline (S. pneumoniae )
ICR (S. pneumoniae, Viridans group, Strep. Gr. C & G)
Minocycline (S. pneumoniae )
Oritavancin
Tedizolid
Telavancin

Trim-sulfa (Enterococcus spp.)

Ofloxacin (S. pneumoniae )
Streptomycin (Enterococcus spp.)
Antifungals
All agents with P. multocida
Benzylpenicillin (CoNS)
Cefoxitin screen (S. saprophyticus and S.
pseudintermedius)

*Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

Sensititre (Thermo Fisher Scientific)

In computer
database
EUCAST terminology
implemented
In reports

Yes

R>

Yes

Yes

IE

Yes

Yes

R>

Yes

Yes

IE

Yes

EUCAST Expert Rules

implemented

All EUCAST rules are implemented in software version 3.3 (2014). There are additional rules,
but these rules are inactivated in the EUCAST setting.

EUCAST organism groups

with no test in the system

N. gonorrhoeae

Agents in EUCAST tables

but not available in the
system

All agents available

Agents available but

EUCAST breakpoints not
implemented in the system

All agents for yeasts and molds

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

MICRONAUT (MERLIN Diagnostika GmbH)

In computer
database
EUCAST terminology
implemented
In reports

Yes

R>

Yes

Yes

IE

No

Yes

R>

Yes

Yes (MICs reported for agents with no EUCAST breakpoints)

IE

No (MICs reported for agents with no EUCAST breakpoints)

EUCAST Expert Rules

implemented

All EUCAST rules are implemented. There are additional rules in the software. Our rules can be
customized by the user.

EUCAST organism groups

with no test in the system

N. gonorrhoeae

Agents in EUCAST tables

but not available in the
system

Ceftolozane-tazobactam (expected during 2016)

Agents available but

EUCAST breakpoints not
implemented in the system

All agents for molds.

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST

Compliance of manufacturers with EUCAST guidelines, 10 March 2016

MIKROLATEST (Erba Mannheim)

In computer
database
EUCAST terminology
implemented
In reports

Yes

R>

Yes

Yes (I)

IE

Yes

Yes

R>

Yes

Yes (I)

IE

Yes

EUCAST Expert Rules

implemented

EUCAST rules are implemented in software version 20151105 (2015). There are additional
rules in the software. The rules can be customized by the user.

EUCAST organism groups

with no test in the system

Gram-positive anaerobes
N. gonorrhoeae

Agents in EUCAST tables

but not available in the
system

All agents for anaerobes, Corynebacterium spp., N. gonorrhoeae,

H. influenzae

Agents available but

EUCAST breakpoints not
implemented in the system

None

Gram-negative anaerobes
H. influenzae

Corynebacterium spp.

The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST