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informative flyer about upcoming events, with descriptive information that substantiates topics that will be covered in the symposium that will take place.
informative flyer about upcoming events, with descriptive information that substantiates topics that will be covered in the symposium that will take place.
informative flyer about upcoming events, with descriptive information that substantiates topics that will be covered in the symposium that will take place.
DEPARTMENT OF IMMUNOLOGY, INSTITUTE OF NEUROIMMUNE PHARMACOLOGY
CENTER FOR PERSONALIZED NANOMEDICINE
Distinguished Seminar Series
Exosomes Produced from HIV Infected Cells Control Immune Regulation
Fatah Kashanchi, Ph.D.
Director of Research, NCBID, School of Systems Biology Professor in the Laboratory of Molecular Virology George Mason University Date: Monday, February 15th, 2016 Venue: AHC-2 453 Time: 1 - 2 pm Refreshments will be served. Abstract: Exosomes are small membrane bound extracellular vesicles that are secreted from the cells into the extracellular environment. In recent years, it has been shown that exosomes can be taken up by recipient cells and can result in phenotypic changes in these cells, which can be either beneficial or detrimental to the cell. These phenotypic changes are associated with the donor cell conditions (infected or uninfected cells), the exosomal content including presence of specific proteins, and non-coding RNAs, as well as the recipient cells external and internal environment. In the case of many viral infections and cancers, exosomes have been implicated in cellular inflammation, antigen presentation, and the transfer of nucleic acids (specifically RNA) and functional proteins between cells. Exosomes have also been reported to participate in the delivery of functional miRNAs and proteins which result in modulation of recipient cell gene expression. Often, this can contribute to disease pathogenesis, especially when exosomes originate from infected cells. In recent years, Kashanchi lab has focused exclusively on exosomes secreted from virally infected cells including HIV and HTLV-1. In multiple independent manuscripts, they have shown how to purify these exosomes from large and small volumes using classical centrifugation methods followed by gradients and nanoparticles, respectively. These exosomes typically range in size from 50-150 nm and are usually secreted using the host ESCART pathway (similar to what most viruses use to exit a cell). In 2015, they also purified exosomes from Rift valley infected cells and Ebola transfected cells focusing on GP, NP, and VP40. The functional comparison between these various exosomes and their effects on pathogenesis and their contribution to disease progression will be discussed. Biography: Dr. Kashanchi trained under the supervision of Dr. C. Wood who worked with Dr. Susumu Tonegawa (Nobel Laureate for Medicine, 1987) at MIT. Dr. Kashanchi further trained in Dr. John Bradys lab at NIH/NCI from 1991-1998 and published more than 30 papers during his postdoc and senior investigator tenure. His research interests include both Genomics and Proteomics of HIV-1 and HTLV-1 infected cells. Research in Kashanchis lab has been funded by various internal and external sources including Selective Excellence, Keck, Snyder, Sinsheimer, Cyclacel, and more than 9 NIH grants, where he is the Principal Investigator. Since his departure from NIH in 1998, Dr. Kashanchi has obtained $14.5 M of independent funding. He has 179 peer-reviewed publications in scientific journals (h index = 45),
12 book chapters, is an NIH study section member (141 panels since 2000), as well as reviewer for 14 different journals.