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and
serology.In
culture
test
there
are
isolation
of
phosphate buffered saline and inoculating into ng into semisolid medium containing
5-fluoruracil.Culture are incubated at 28o to 30o and examined weekly by dark field
microscopy for up to 13 weeks before being discarded[2].Identification of leptospiral
is task for specialized reference laboratories.For complete identification,a
combination of procedures is used to determine 1) if the isolate is a pathogen or a
saprophyte; 2) the species of Leptospira to which the isolate belongs; and 3) the
serogroup and serovar of the isolate.Isolated leptospires are identified by serological
methods or by recently developed molecular techniques [2],which can perform the
microscopic agglutination test to identify isolates with relative rapidity.Susceptibility
testing is the test to know leptospires susceptibility with.Leptospires are susceptible
to
beta-lactams,macrolides,tetracyclines,fluoroquinolones
and
streptomycin[1]
diagnosis
of
leptospiral
2006;54(April):339-340.
infection.
Assoc
Physicians
India.