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Faculty of Medicine
Hasanuddin University
Biochemistry of
Digestive System
Ika Yustisia
August 2010
Contents
1. Introduction
2. General Principles of Digestion
3. Digestive enzymes
4. Digestion and Absorption of Carbohydrates
5. Digestion and Absorption of Lipids
6. Digestion and Absorption of Proteins
7. Clinical correlations
7.1. Lactose Intolerance
7.2. Malnultrition in Advanced Cancer and AIDS Patients
8. Conclusion
1. Introduction
Biochemistry is the study of the molecular events that correspond to
the phenomenon of life. One of the important phenomenon in the body is the
mechanism of digestion and absorption. Digestion is considered as the
degradation of the nutrient molecules into components simple enough to be
subsequently absorbed in the intestine. While absortion is the uptake of
digested
components
by
the
intestinal
cells
(enterocytes).
The
gastrointestinal (GI) tract with the organs functionally associated with it, are
responsible for the process of digestion and absorption.
Beside its association to the gastrointestinal tract in digestion and
absorption, liver has lots of function that plays a vital role in life of the
organism. It plays in intermediary metabolism, in the detoxification of foreign
toxic compounds including drugs, degradation of bilirubin, synthesis of
proteins, and as a strorage organ.
This hand out will be discussing about the biochemistry of the
digestive system especially about the digestive enzymes, epithelial transport,
and the digestion and absorption of the major nutrients. Some important
functions of the liver considered to be discussed in this handout, that have
not been studied in the other subjects/systems. Clinical correlations are given
in other to make a comprehensive understanding about the biochemistry of
the digestive system and the liver.
2. General Principles of Digestion
Considering to the definitions as mentioned above, the process of
digestion is characterized by several specific stages, which occur in
characteristic sequence, allowing the interaction of fluid, pH, emulsifying
agents, and enzymes. This requires concerted actions of the liver,
pancrease, gall bladder, and salivary glands. The process that are involved
can be summarized as follows1:
1. Lubrications and homogenizations of food with fluids secreted by glands
of the GI tract, starting in the mouth.
Organ
Salivary glands
Stomach
Pancrease
Liver
Gallblader
Small intestine
Large intestine
Primary function
Production of fluid and digestive enzymes for
homogenization, lubrication, and digestion of
carbohydrate (amylase) and lipid (lingual lipase)
Secretion of HCl and proteases to initiate hydrolysis
of proteins
Secretion of HCO3-, proteases, lipases, and
amylase to continue digestion of protein, lipid, and
carbohydrate respectively
Elaboration of bile acids
Storage and concentration of bile
Final intraluminal digestion of foodstuffs, digestion
of carbohydrate dimers and specific absorptive
pathway for digested material
Absorption of fluid and electrolyte and products of
bacterial action in colon
cleaves
-1,4
glycosidic
bonds
in
starch
(further
the normal salivary pH of 6.5 and 7.0 but is rapidly denatured in the acidic
environment of the stomach. Therefore, it makes only a minor contribution to
strach digestion. Its main function is to keep the teeth clean by dissolving
starchy bits of food that remain lodged between the teeth after a meal. 3
Lysozyme hydrolyzes -1,4 glycosidic bonds in the bacterial cell wall
polysaccharide peptidoglycan. Lysozyme kills some types of bacteria; others
are resistant because their peptidoglycan is protected from the enzyme by
other cell wall components or, in the case of the gram negative bacteria, by
an overlying outer membran. The members of the normal bacterial flora in
the mouth are also resistant to lysozyme. However, many bacteria from other
ecosystems are killed by lysozime while. 3
3.2. The stomach
In the stomach the food is mixed, stored for some time then finally
discharge into the duodenum. The stomach secretes enzymes, intrinsic
factor, and hydrochloride acid. The secretion of gastric juice is controlled by
neural mechanism involving the vagus nerve and by gastrin, the hormone of
the stomach.4
Gastrin is a polypeptide hormone produced and stored by the G cells
found in the antrum of the stomach and in the proximal duodenum. Gastrin
secretion is stimulated by the distension of the stomach, the presence of
proteins and polypeptide in the stomach, vagal stimulation, plasma calcium
concentration, and circulating catecholamines. Its secretion is inhibited by
acid in the antrum and by blood-borne factors such as secretin, gastric
inhibitory protein (GIP), vasoactive intestinal peptide (VIP), glucagon, and
calcitonin. Gastrin stimulates secretion of acid, pepsinogen (read the
zymogens), and intrinsic factor; increases gastric motility; and stimulates the
growth of gastric mucosa. Gastrin increases acid secretion and the acid then
feeds back to inhibit further gastrin secretion.
sterol esterase. Bile salts activate the lipid cleaving enzymes through micelle
formation.3,5
Several
hydrolasesparticularly
ribonuclease
(RNAse)
and
the
microvilli
glycosidases,
etc.),
of
the
these
intestinal
enzymes
epithelium
ensure
(peptidases,
almost
complete
3.5. Zymogens
Some digestive enzymes are potentially so damaging to the cells that
synthesizes them that they are secreted as inactive precursors or zymogens.
Among the digestive enzymes, the proteases and phospholipases are
dangerous. Once secreted, these zymogens are converted into their active
forms. This activation
bonds is
about
1:20.
Glycogen
is
the
animal
storage
are
two
separate
mechanisms
for the
absorption
of
10
adult
human
ingests
about
60-150g
of
lipid
per
day.
Triacylglycerols constitute more than 90% of this intake. The rest is made up
of phosphoslipids, cholesterol, cholesteryl ester, and free fatty acids. In
addition, 1-2g of cholesterol and 7-22g of phosphatidylcholine (lecithin) are
secreted each day by the liver and reach the small intestine with bile. 2
The poor water solubility of lipids presents problems for digestion
because substrates are not easily accessible to the digestive enzymes in the
aqueous phase. In addition, most products of lipid digestion are themselves
lipid with poor water solubility so that they tend to form aggregates that
hinder effectives absorption. These problems are overcome by (a)
generating/secreting surfactive molecules than increase the interfacial area
between aqueous and lipid phases and (b) solubilization of lipids with
detergents. Thus changes in the physical state of lipids are intimately
connected to chemical changes during digestion and absorption. 2
Hydrolysis of triacylglycerols is initiated by lingual and gastric lipases,
which attack the sn-3 ester bond forming 1,2 diacylglycerols and free fatty
acids, aiding emulsification. Pancreatic lipase is secreted into the small
intestine, and requires a further pancreatic protein, colipase, for activity. It is
specific for the primary ester links-ie, positions 1 and 3 in triacylglycerolsresulting 2-monoacylglycerols and free fatty acids as the major end products
of luminal triacylglycerol digestion. Monoacylglycerols are poor substrates for
11
by
cholesterol
acyltransferase.
The
newly
synthesized
12
or
the
amino
terminus
(aminopeptidases).
13
14
for a variety of reasons (genetic defect, physiological decline with age, or the
result of injuries to the mucosa). Lactase is the most commonly deficient
enzyme. Absolute or relative deficiency is experienced as milk intolerance. 2
Consequences of a lack of lactose hydrolysis in the upper small
intestine are inability to absorb lactose, which then becomes available for
bacterial fermentation in the lower small intestine. Bacterial fermentation
produces gas (distention of gut and flatulence) and osmotically active solute
that draw water into the intestinal lumen (diarrhea). Lactose in yogurt has
already been hydrolized during the fermentation process of making yogurt.
Thus, individuals with lactase deficiency can usually tolerate yogurt better
then unfemented dairy products.2
7.2. Malnultrition in Advanced Cancer and AIDS Patients
Patients with advanced cancer, HIV infection and AIDS, and a number
of other chronic disease are frequently undernourished, a condition called
cachexia. Physically, they show all the signs of marasmus, but there is
considerably more loss of body protein than occurs in starvation. The
secretion of cytokines in response to infection and cancer increases the
catabolism of tissue protein. This differ from marasmus, in which protein
synthesis
is
reduced,
but
catabolism
in
unaffected.
Patients
are
of
fatty
acids
from
adipose
tissue
and
ATP-expensive
15
References:
1. Broom, I. Function
of the
Gastrointestinal Tract in
Medical
16
17