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ORIGINAL ARTICLE
Revised: 15 August 2011 / Accepted: 26 August 2011 / Published online: 8 September 2011
# Association of Food Scientists & Technologists (India) 2011
B. S. Ogunsina (*)
Department of Agricultural and Environmental Engineering,
Obafemi Awolowo University,
Ile Ife, Nigeria
e-mail: bsogunsina@yahoo.com
T. N. Indira : C. Radha
Department of Protein Chemistry and Technology, Central Food
Technological Research Institute (CSIR Constituent Laboratory),
Mysore 570020, India
A. S. Bhatnagar : S. Debnath : A. G. Gopala Krishna
Department of Lipid Science and Traditional Foods, Central Food
Technological Research Institute (CSIR Constituent Laboratory),
Mysore 570020, India
Introduction
Vegetable oils constitute an important part of human
livelihood all over the world. The widening gap between
demand and supply necessitates the need for alternate
sources of edible oils to augment global production.
Moringa oleifera seeds contain about 42% of a brilliant
yellow, high-oleic acid crude oil having a pleasant, peanutlike flavor. The oil consists of 82% unsaturated fatty acids,
70% of which is oleic acid (Tsaknis et al. 1998; Foidl et al.
2001; Farooq et al. 2006; Rahman et al. 2009). Apart from
the emphasis of modern nutrition on oils that contain high
amount of unsaturated fatty acids, high oleic acid oils are
known to be healthy alternatives to hydrogenated vegetable
oils (Tsaknis et al. 1998; Abdulkarim et al. 2005; Farooq et
al. 2006; Rahman et al. 2009). Olive oil, the widest known
in this category, is seldom used for frying because of its
high cost. There are several reports on the composition and
characteristics of Moringa oleifera seed oil varieties from
different countries of origin, e.g., India (Lalas and Tsaknis
2002), Kenya (Tsaknis et al. 1999a, b), Malawi (Tsaknis et
al. 1998), Malaysia (Abdulkarim et al. 2005), Pakistan
(Farooq et al. 2006; Manzoor et al. 2007), Bangladesh
(Rahman et al. 2009) considering its prospect as an
alternative vegetable oil source. Quality characteristics
of moringa seed oils from Indian cultivar (PKM-1) and
an African cultivar grown in similar agro-climatic
conditions of Argentina has also been reported (Ayerza
2011). Moreover, very recent studies on Moringa oleifera
seed oil have been conducted to explore the possibility of
504
505
506
Table 1 Physico-chemical characteristics of cold pressed moringa seed oil (CPMSO) and hexane extracted moringa seed oil (HEMSO)
Parameters
CPMSO
30.0a 1.0
(25 Y & 1 R)
3.5a 0.12
1.0a 0.01
HEMSO
36.0b 1.2
(30 Y & 1.2 R)
4.0b 0.05
1.02a 0.01
Moringa oleifera
Periyakulam-1
seed oil (Indian origin)
(Lalas and Tsaknis 2002)
Moringa concanensis
seed oil (Pakistan
origin) (Latif and
Anwar 2008)
Moringa oleifera
Lam. seed oil
(Bangladesh origin)
(Rahman et al. 2009)
39.0
29.5
37.8
1.1
NR
0.32
NR
0.7
1.5
66.7
68.9
67.8a 0.42
68.5a 0.36
65.6
190.4a 0.57
191.2a 0.52
188.0
180
180.0
0.59a 0.05
95.5a 1.0
3.1a 0.05
1.47a 0.001
0.65a 0.03
90.2a 1.7
0.0b
1.47a 0.001
NR
35.4
NR
1.46
0.76
120.4
NR
1.46
0.77
251.5
NR
1.46
0.90a 0.01
0.93a 0.01
43.8a 0.14
0.92a 0.01
0.90a 0.01
43.6a 0.25
0.91
NR
45.1
0.87
NR
NR
0.90
56.5
NR not reported
Values on the same row followed by different superscripts differ significantly at p0.05
Samples were taken in triplicate and analysis carried out in duplicate making six determinations (n=6) and mean standard deviation value
reported
507
Table 2 Fatty acid composition of cold pressed moringa seed oil (CPMSO), hexane extracted moringa seed oil (HEMSO) and commercial raw
groundnut oil (GNO)
Fatty acid
Composition
(relative area %)
Myristic (C14:0)
Palmitic (C16:0)
Palmitoleic(C16:1)
Stearic (C18:0)
Oleic (C18:1)
Linoleic (C18:2)
Linolenic (C18:3)
Arachidic (C20:0)
Gadoleic (C20:1)
Behenic (C22:0)
CPMSO
0.24a 0.05
5.8a 0.11
1.2a 0.35
3.9a 0.54
79.5a 1.11
ND
2.2a 0.62
2.2a 0.43
ND
5.1a 0.47
% SFA
% MUFA
% PUFA
17.2a
80.7a
2.2a
HEMSO
Moringa oleifera
Periyakulam-1 seed
oil (Indian origin)
(Lalas and Tsaknis 2002)
Moringa concanensis
seed oil (Pakistan
origin) (Latif and
Anwar 2008)
Moringa oleifera
Lam. seed oil
(Bangladesh origin)
(Rahman et al. 2009)
0.1
6.5
1.5
5.9
71.2
0.7
0.2
3.6
2.2
6.4
NR
11.9
2.4
3.6
67.3
1.8
NR
3.7
1.8
7.6
0.1
6.2
1.1
4.8
74.4
1.2
0.3
3.5
1.6
6.2
22.5
74.9
0.9
26.8
71.5
1.8
20.8
77.1
1.5
0.72b 0.13
6.1a 0.24
1.2a 0.0
4.6b 0.22
78.7a 1.21
ND
1.8a 0.34
2.3a 0.05
ND
4.5a 1.53
18.3a
79.9a
1.8a
*GNO
ND
14.51.07
ND
4.70.31
44.71.21
30.51.75
1.20.22
4.40.67
ND
ND
23.9
44.4
31.7
NR not reported
ND not detected
*Fatty acid composition of GNO and RGNO was similar
Values on the same row followed by different superscripts differ significantly at p0.05
Samples were taken in triplicate and analysis carried out in duplicate making six determinations (n=6) and mean standard deviation value
reported
it increased from 1.2 meq O2/kg on day zero to 5.6 meq O2/kg
on the 35th and 42nd days showing a relatively better stability
against oxidation. The fatty acid composition of CPMSO and
GNO suggested that they contained 31.7 and 2.2% of
polyunsaturated fatty acids (PUFA). The steady rise in the
peroxides in GNO can be attributed to the presence of more
amounts of PUFA than in CPMSO since the oxidative
stability of oil is inversely proportional to its PUFA content
(Bhatnagar et al. 2009).
Stability of CPMSO
Oxidative stability Changes in PV of CPMSO and GNO
during incubation at 37 C and 55% RH are presented in
Fig. 1. The results indicated greater inhibitory effect of
CPMSO against formation of peroxides than GNO. The PV
of GNO increased steadily from 3.0 meq O2/kg on day zero
to 26.93 meq O2/kg on the 42nd day, whereas for CPMSO,
508
Table 3 Thermal stability and frying stability of cold pressed moringa seed oil (CPMSO) and commercial refined groundnut oil (RGNO)
Duration of thermal
treatment (h)
Peroxide value
(meqO2 / Kg)
0
6
12
18
24
30
36
30.01.0 a
50.01.5 b
65.01.5 c
75.02.0 d
79.01.0 d
80.01.0 d
80.01.0 d
1.50.05
2.10.05
2.50.05
3.10.05
4.10.03
4.90.05
5.60.14
30.01.0 a (20Y+2R)
40.01.5 b (25Y+3R)
3.50.05 a
3.40.05 b
3.00.03 c
2.70.03 d
2.40.03 e
2.30.01 f
2.20.01 g
CPMSO
3.50.01 a
4.90.01 b
Before frying
After frying
1.000.01
10.20.24
Before frying
After frying
12.00.5 c (7Y+1R)
251.0 d (15Y+2R)
RGNO
0.720.01 c
1.40.02 d
1.50.01
21.90.10
(25.0Y
(35.0Y
(40.0Y
(43.0Y
(47.0Y
(48.0Y
(48.0Y
&
&
&
&
&
&
&
1.0R)
3.0R)
5.0R)
6.4R)
6.4R)
6.4R)
6.4R)
b
c
d
e
f
g
Viscosity (mPa.s)
43.80.55
107.31.53
115.01.22
197.01.81
234.72.14
241.41.46
337.51.97
a
b
c
d
e
f
g
43.82.56 a
65.13.07b
45.32.06
50.22.04
c
d
Total polar
materials (%)
3.10.14
6.80.19
12.20.37
16.00.39
27.50.56
46.50.58
65.80.97
a
b
c
d
e
f
g
3.10.16a
90.80.98 b
1.10.17
95.21.45
c
d
Values on the same column followed by different superscripts differ significantly at p0.05
Samples were taken in triplicate and analysis carried out in duplicate making six determinations (n=6) and mean standard deviation value
reported
Conclusions
The present study provides information on the extraction,
quality and stability of Moringa oleifera Jaffna variety seed
oil which has not been reported so far. The quality
characteristics of the cold pressed and solvent extracted
Moringa oleifera variety Jaffna seed oil such as peroxide
value, saponification value, unsaponifiable matter, refractive
index, density and specific gravity were found to be almost
similar. Moringa oleifera Jaffna variety seed oil, based on its
high oleic acid content can become another valuable source
of high-oleic acid oil. The high oleic acid content also
provides good stability to Moringa oleifera seed oil. The oil
had good thermal stability. The oxidative stability and
frying stability of cold pressed Moringa oleifera Jaffna
variety seed oil was better than commercial raw and
refined groundnut oils respectively. Based on the present
findings, Moringa oleifera Jaffna variety seed oil has
shown enough promise to be considered as more stable
and healthy substitute for commercial groundnut oil as a
cooking and frying medium.
Acknowledgements This research work was funded by the United
Nations University and Central Food Technological Research Institute
(CFTRI), Mysore, India. Authors acknowledge Dr V. Prakash,
Director, CFTRI, for providing infrastructural facilities. Thanks are
due to Dr A. G. Appu Rao, Head of Protein Chemistry and
Technology and Dr B.R. Lokesh, Head of Lipid Science and
Traditional Foods for their valuable suggestions.
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