Phosphine Resistance

Test Kit
Directions for Use

Content
1 Important notes
1.1 Address
1.2 General information
1.3 Liability

3
3
3
3

Name of appliance

Field of application

Equipment

Filling

Safety measures

Starting

Pictogram

Evaluation

10 How to adjust the concentration in the syringe

11 How to measure the phosphine concentration in the canister 6

12 Cleaning of the canister

13 Dosage

14 Testing the tightness of the canister

15 Limitation

Appendix 1
Appendix 2
Appendix 3
Appendix 4

Table to determine the dilution

Diagram to determine the dilution
Table of the time periods
Test Report Resistance Test Kit

8
9
10
11

1
1.1

Important notes
Address

Detia Degesch GmbH

Dr.-Werner-Freyberg-Str. 11
D-69514 Laudenbach
Phone:
+49 (0)6201 708 401
Fax:
+49 (0)6201 708 402
www.detia-degesch.de
info@detia-degesch.de
1.2

General information

These instructions for use apply to the PHOSPHINE RESISTANCE TEST KIT.
It should be kept at a place accessible to all users. The instructions facilitate the
safe and proper transport, start-up and servicing of the PHOSPHINE
RESISTANCE TEST KIT. Please read these instructions prior to transporting,
starting-up or servicing the appliance. Adhere to the instructions. We reserve the
right to make technical alterations to the appliance described in these instructions.
The technical data and measurements are not binding No claims can be based
thereon.
We reserve the copyright of these instructions and the enclosed drawing as well as
of any other relevant papers.
1.3

Liability

We accept no liability for any damage or operational stoppage caused by nonadherence to these instructions or any improper repairs.
The manufacturer is not liable for damages occurring during the guarantee-period
which are caused by:

improper handling

incorrect set-up

unauthorized alterations or non-adherence to the manufacturers

recommendations or damages thus caused

using accessories or spare parts which are not supplied or

recommended by the manufacturer.

Name of appliance

PHOSPHINE RESISTANCE TEST KIT

Field of application

The PHOSPHINE RESISTANCE TEST KIT is intended for testing the behaviour
and the activity of insect pests particular in stored products in a defined phosphine
containing atmosphere.
Any other uses beyond the above stated are classed as improper use.
4

Equipment

100 ml syringe
2 cannula: 1 cannula with a rubber hose
5 l flexible plastic canister in a cardboard
lid with septum
5 x 2 TEST KIT PELLETS in an aluminium foil pouch
diagram and table to determine the dilution
instructions for use

Filling

Insert only 2 TEST KIT PELLETS (= one aluminium foil pouch) into the flexible
canister.
Do not overfill the canister.
6

Safety measures

Please adhere to the instructions for the protection of applicators as stated in the
directions of use of the fumigant and use the personal protective equipment
recommended.
Do not eat, drink or smoke while handling the fumigant. After completion of the
work wash hands thoroughly. Keep both fumigant and appliance out of the reach
of children.
7

Starting
1)
2)
3)
4)
5)

unfold the plastic canister

add 50 ml water (I)
put 2 TEST KIT PELLETS in the canister (I)
close the canister immediately with the lid
shake the canister carefully (the pellets are decomposed after 5 min)
(II)
6) connect a testing tube with the canister by using the cannula with the
rubber hose (III)
7) determine the phosphine concentration in the canister

8)
9)
10)
11)
12)
13)
14)
15)
8

use the diagram to determine the dilution for a concentration of 3000

ppm in the syringe
remove the butt of the syringe (IV)
add 20 insects of the same species into the syringe (IV)
push the butt carefully into the syringe (V)
adjust the air volume in the syringe (V)
connect the syringe with the canister (cannula and adapter) (VI)
fill the syringe up to 100 ml (VI)
observe the behaviour and activity of the insects
Pictogram
insect pests

2. pellets

1. water

phosphine
5 min
I.

II.

III.

IV.

V.

VI.

Evaluation

In case there are any active insects after a given time period and a phosphine
concentration of 3000 ppm, the tested insect strain is resistant to phosphine. The
time periods for several species of insects are given in the table of Appendix 3.
Resistant insect strains have to be fumigated with a higher concentration and a
longer exposure time. Please consider the GOOD FUMIGATION PRATICE
recommended by the Detia Degesch GmbH.
If you find a resistant insect strain please send a sample (100 200 insects)
together with a copy of your test report (Appendix 4) to the Detia Degesch GmbH.
10

How to adjust the concentration in the syringe

The two TEST KIT PELLETS generate a phosphine concentration of

approximately 5000 6000 ppm in the canister. Therefore you have to dilute this
atmosphere with air to get 3000 ppm in the syringe.

100,0
95,0
90,0
85,0
80,0
75,0
70,0
65,0
60,0
55,0
50,0
45,0
40,0
35,0
30,0
25,0
20,0
15,0
10,0
5,0
0,0
2500

3000 3500

4000 4500

5000 5500

6000 6500

7000 7500

8000 8500

0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
45,0
50,0
55,0
60,0
65,0
70,0
75,0
80,0
85,0
90,0
95,0
100,0
9000 9500 10000

volume air [ml]

take out from the canister [ml]

First, determine the phosphine concentration in the canister with testing tubes (eg
5000 ppm). Then read this concentration on the x-axis and draw a straight
perpendicular line (red line in the diagram).

concentration in the canister [ppm]

After that draw two horizontal lines from the point of intersection between the red
and dark blue line, one to the left (green line in the diagram) and one to the right
(blue line in the diagram). The green line yield the volume you have to take out of
the canister and the blue line yield the volume air. The sum of both is 100 ml and
adjust a concentration of 3000 ppm in the syringe.
11

How to measure the phosphine concentration in the canister

Connect the cannula with the rubber hose, the testing tube and the pump. Than
prick the cannula into the septum of the canister and determine the phosphine
concentration as described in the instruction for the testing tubes.
12

Cleaning of the canister

To remove the phosphine containing atmosphere in the canister, fill it completely

with water. After few minutes empty the canister and repeat the procedure. Dry the
canister in the air.
13

Dosage

The two TEST KIT PELLETS with 8 % magnesium phosphide generate a

phosphine concentration of approximately 5000 6000 ppm. This is the maximum
dosage. Do not use more than two pellets.

14

Testing the tightness of the canister

We recommend to test the tightness of the canister before each use.

Unfold the canister and close it with the lid. Than press on both sides of the
canister and look/hear if any air leaves the canister.
15

Limitation

The PHOSPHINE RESISTANCE TEST KIT is not designed for the use with other
metal phosphide containing products like Phostoxin or Magtoxin. Their higher
active ingredient content develops a phosphine concentration in the canister above
the explosion limit.

Appendix 1: Table to determine the dilution

concentration in the canister take out from the canister volume air
[ppm]

[ml]

[ml]

3000

100,0

0,0

3250

92,3

7,7

3500

85,7

14,3

3750

80,0

20,0

4000

75,0

25,0

4250

70,6

29,4

4500

66,7

33,3

4750

63,2

36,8

5000

60,0

40,0

5250

57,1

42,9

5500

54,5

45,5

5750

52,2

47,8

6000

50,0

50,0

6250

48,0

52,0

6500

46,2

53,8

6750

44,4

55,6

7000

42,9

57,1

7250

41,4

58,6

7500

40,0

60,0

7750

38,7

61,3

8000

37,5

62,5

Appendix 2: diagram to determine the dilution

Appendix 3: Time periods to determine the susceptibility of insects

Species

Time period
[min]
11
13
12
8
8
15

Oryzaephilus surinamensis
Cryptolestes ferrugineus
Sitophilus granarius
Tribolium castaneum
Lasioderma serricorne
Acanthoscelides obtectus
This table will be revised if new results were developed.

10

Appendix 4:
1

Test-Report Resistance Test Kit

General Informations
Name of user:
Country/region:
Date:

Resistance Test
Pest:
Concentration in the plastic container [ppm]:
Volume air [ml]:
Temperature during test:

Active beetles after:

5 min

20 min

45 min

10 min

25 min

60 min

15 min

30 min

90 min

Fumigation Conditions

Dosage of fumigation:
Exposure time:
Application (eg. Container, store-room etc.)
Comment to the test:

PLEASE RETURN TO DETIA DEGESCH GMBH!

11

Appendix 4:
1

Test-Report Resistance Test Kit

General Informations
Name of user:
Country/region:
Date:

Resistance Test
Pest:
Concentration in the plastic container [ppm]:
Volume air [ml]:
Temperature during test:

Active beetles after:

5 min

20 min

45 min

10 min

25 min

60 min

15 min

30 min

90 min

Fumigation Conditions

Dosage of fumigation:
Exposure time:
Application (eg. Container, store-room etc.)
Comment to the test:

PLEASE RETURN TO DETIA DEGESCH GMBH!

12

Appendix 4:
1

Test-Report Resistance Test Kit

General Informations
Name of user:
Country/region:
Date:

Resistance Test
Pest:
Concentration in the plastic container [ppm]:
Volume air [ml]:
Temperature during test:

Active beetles after:

5 min

20 min

45 min

10 min

25 min

60 min

15 min

30 min

90 min

Fumigation Conditions

Dosage of fumigation:
Exposure time:
Application (eg. Container, store-room etc.)
Comment to the test:

PLEASE RETURN TO DETIA DEGESCH GMBH!

13

Appendix 4:
1

Test-Report Resistance Test Kit

General Informations
Name of user:
Country/region:
Date:

Resistance Test
Pest:
Concentration in the plastic container [ppm]:
Volume air [ml]:
Temperature during test:

Active beetles after:

5 min

20 min

45 min

10 min

25 min

60 min

15 min

30 min

90 min

Fumigation Conditions

Dosage of fumigation:
Exposure time:
Application (eg. Container, store-room etc.)
Comment to the test:

PLEASE RETURN TO DETIA DEGESCH GMBH!

14

Appendix 4:
1

Test-Report Resistance Test Kit

General Informations
Name of user:
Country/region:
Date:

Resistance Test
Pest:
Concentration in the plastic container [ppm]:
Volume air [ml]:
Temperature during test:

Active beetles after:

5 min

20 min

45 min

10 min

25 min

60 min

15 min

30 min

90 min

Fumigation Conditions

Dosage of fumigation:
Exposure time:
Application (eg. Container, store-room etc.)
Comment to the test:

PLEASE RETURN TO DETIA DEGESCH GMBH!

15