CANNABIS OIL

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INTRODUCTION

Group 1 1J-PH

CANNABIS OIL

CHEMICAL EVALUATION OF AN UPCOMING

CANNABIS-BASED MEDICINE
BY GROUP 1
1JPH

Adloc, Jessa Grace

Aguilar, Patriz Michelle
annabinoids are known to exert palliative effects in cancer
Bajar, Trizia Kaye
patients such as reducing nausea, vomiting and pain, and
Canlas, Glen Katrine
Capiral, Joseph Edwardstimulating appetite. Preclinical evidence has also shown
Capulong, Hazel Joy cannabinoids being capable, under some conditions, of
Carlos, Allyson Denise inhibiting the growth and development, and arresting the cell
Casao, Elaine Andrea
Chong, Patricia Danielleof cancer cells.

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INTRODUCTION

INTRODUCTION

Cannabis oil, a concentrated extract from the buds and leaves of the
cannabis plant. Various non-polar solvents have been recommended
for this purpose, including petroleumether, naphtha (diesel or
kerosene), alcohol and olive oil. The purpose of the extraction, is to
make cannabinoids and other beneficial components such as terpenes
available in a highly concentrated form. Preparation methods for
Cannabis oil are relatively simple and do not require particular
instruments.

Rick Simpson a Canadian who claims to have cured his skin

cancer through repeated topical application of Cannabis oil. In
his website and documentary, Run from the Cure,
it is explained in detail how to prepare and administer the
product.

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cycle

CANNABIS OIL

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INTRODUCTION
With a growing interest in Cannabis oil for self-medication it is important not to
overlook the importance of quality control and standardization.

KEYWORDS

This research evaluates the effects of preparation methods, and

particularly the solvents used, on the final composition of the
different Cannabis oils. The obtained results are not intended to
support or deny the therapeutic properties of these products, but
may be useful for better understanding the experiences of selfmedicating patients through chemical analysis of this popular
medicine.

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CANNABIS

CANNABINOIDS
affect the user by interacting
with specific receptors, located
within different parts of the
central nervous system

commonly known as
MARIJUANA or HEMP

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CANNABIS OIL

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TERPENES

CANCER

group of diseases involving

abnormal cell growth with the
potential to invade or spread
to other parts of the body

volatile unsaturated
hydrocarbons found in the
essential oils of plants,
especially conifers and citrus
trees

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RICK
SIMPSON
OIL

CANNABIS OIL
a thick, sticky,
resinous substance made up
of cannabinoids, such as THC
(tetrahydrocannabinol) and
CBD (cannabidiol), that is
extracted from the cannabis
plant

Named after Rick Simpson, a

medical marijuana activist, who
has been providing people with
information about the healing
powers of Hemp Oil medications
for nearly a decade now.

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SIGNIFICANCE
OF THE STUDY

STATEMENT OF
THE PROBLEM

present a chemical analysis of this popular medicine that would further lead to a

Among
the self-medicating population, these products are capable of curing
cancer. However, this claim is only backed up by anecdotal patient stories; none
of the production methods for Cannabis oil have been validated in published
literature, and no reports have been made on the chemical composition of these
products either. Hence, no one seems to know what is actually in it. Therefore,
recognizing the need for more information on quality and safety issues
regarding Cannabis oils, an analytical study was performed to compare several
generally used preparation methods on the basis of content of cannabinoids,
terpenes, and residual solvent components.

better understanding of the experiences of the affected population

educate and give awareness to the patients of what exactly they are taking
allow the researchers to know the active ingredient/s of herbal cannabis and its
possible therapeutic effects upon a patients intake

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SCOPE AND
LIMITATIONS

SCOPE AND
LIMITATIONS

Cannabis plant material used in this study was of the variety

Only female flower tops were used (Cannabis Flos).

Bedrocan (licensed company in Europe to grow cannabis)

In Europe, they were used at the end of the 19th centuryto treat pain,

Cannabis-based medications have been a topic of intense study

spasms, asthma, sleep disorders, depression, and loss of appetite.

since the endogenous cannabinoid system was discovered two

decades ago

Medications based on cannabis have been used for therapeutic

purposes in many cultures for centuries

It was cultivated under standardized conditions according to the

requirements of Good Agricultural Practice (GAP).

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CANNABIS OIL

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METHODOLOGY

METHODOLOGY

PLANT MATERIAL
Cannabis plant used in this study was of the variety of "Bedrocan" which produces
strains of cannabis that contains 19% of tetrahydrocannabinol. This is obtained from
Bedrocan BV the only licensed company in Europe to produce cannabis for
medicinal use.
Only female flowers were used (Cannabis Flos). After harvest, the plant material
was air-dried in the dark at constant temperature and humidity for 1 week. Dried
flowers were manicured to remove leaves and stems, and finally cut into smaller
pieces.

CHEMICAL SOLVENTS
ethanol (HPLC grade), methanol (HPLC grade), acetic acid( analytical grade), and
activated charcoal(analytical grade).
Petroleum ether (boiling point 40-65C; analytical grade). Naphtha (light
hydrotreated petroleum distillate), Olive oil (extra virgin quality), Pure ethanolic
standards for delta-9-tetrahydrocannabinol (THC) and delta-9tetrahydrocannabinolic acid (THCA) were produced as previously described.
Cellulose filter paper for filtration of extracts.

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METHODOLOGY
PREPARATION STEP
PREHEATING
PREPARATION OF CONCENTRATED EXTRACTS
GC/FID ANALYSIS
HPLC ANALYSIS
NMR ANALYSIS

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EFFECTS OF PREHEATING

RESULTS
AND
DISCUSSIO
N
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ANALYSIS OF THE
EXTRACTS: CANNABINOID
AND TERPENE CONTENT

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RESIDUAL SOLVENT
TESTING

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RECONFIRMATION USING AN
ACTUAL PATIENT SAMPLE
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CONCLUSION
Beneficial natural products such as "-sitosterol", terpenes,

cannabinoids, phenolica including methyl salicylate were detected.

When diets are well-supplemented with Omega-6 and Omega-3

PUFA in the proper 3:1 ratio, numerous health benefits are achieved
To date, there has been no reported cases of toxicity from the

ingestion of hemp seed oil

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