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Abstract
Snakehead fish, also known as Haruan, is recognized in Asia Pacific countries as a remedy for healing of wounds. The fish
enhances dermal wound healing and reduces post-operative pain and discomfort. The efficacy of wild type snakehead fish has
made it a common food served to women after childbirth or those who had undergone surgery. Due to high demands of snakehead
fish, farming of the fish is now carried out commercially. However, the flesh of cultured snakehead fish has been said to produce
different texture from the wild type fish. In this study, analysis of the protein composition of the flesh of snakehead fish was
carried out. Wild type snakehead fish of different sizes and caught in different months of the year were compared. The data
showed that fish of smaller sizes yielded higher protein content as compared to the bigger fish. However, protein profiles of the
fish were similar for all the different months of catching. The major group of protein in snakehead fish was enzymes, followed by
structural proteins. The protein profile displayed may be used as a reference for farming and culturing of snakehead fish.
Keywords: Snakehead fish, proteomic
Introduction
26
27
25.00
20.00
ab
ab
ab
b
b
ab
b
a
ab
ab
ab a
a
a
ab
ab
15.00
B1
B2
B3
10.00
500.0
ab
a
ab
5.00
Content (mg / g)
30.00
400.0
300.0
200.0
100.0
0.00
0.0
16
23
24
25
28
Length (cm)
29
30
38
B1
B2
B3
Batch Number
28
Table 1: List of water soluble proteins detected in Haruans muscle tissue. Band number are refer to indication mentioned
in Figure 3.
SWISS-PROT
Accession number
Protein Name
Mw
pI
Function
Band number
KIBOA/ P00570
21761
8.94
Enzyme
14
Q804Y1
Aldolase (Fragment)
17427
8.73
Enzyme
10
Q8JH72
Aldolase A.
40223
8.27
Enzyme
2,8,9,10
Q7ZW73
Aldolase b, fructose-bisphosphate
39700
8.75
Enzyme
10
Q6PUS4
97916
6.11
Enzyme
2,7
Q9YGE7
85034
5.90
Enzyme
1,6
Q7ZU04
43178
5.49
Enzyme
7,9
Q804Z1
Creatine kinase
43231
6.29
Enzyme
Q804Z2
Creatine kinase
43032
6.32
Enzyme
Q9I8I6
46859
8.73
Enzyme
S13164
43267
6.20
Enzyme
7,9,10
Q98SS7
29125
8.89
Enzyme
7,9
Q9DFM2
21139
5.79
Enzyme
Q7T1J1
42608
5.89
Enzyme
Q7T306
43115
6.29
Enzyme
Q9YI16
42983
6.21
Enzyme
2,9
Q9YI15
43133
6.22
Enzyme
Q9YI14
43185
6.25
Enzyme
Q7T1J0
47108
8.50
Enzyme
Q7T1J3
42713
6.32
Enzyme
9,10
Q7T1J2
42888
6.44
Enzyme
Q7ZZM5
Enolase (Fragment)
28757
8.15
Enzyme
Q6TH14 (AAQ97775)
47848
Enzyme
Q6GQM9
Enolase 2
47160
4.77
Enzyme
O57518
39957
6.21
Enzyme
10
Q76BF6
41657
6.04
Enzyme
Q8AY84
11317
4.67
Enzyme
Q6NXD1
PKM 2 protein
58598
6.36
Enzyme
Q803D2
47080
6.97
Enzyme
Pol-like protein
149432
9.28
Enzyme
11,12
Q7SXV3
60118
7.30
Enzyme
Q8JJC2
Pyruvate kinase
58767
6.35
Enzyme
Q8QGU8
Pyruvate kinase
58582
7.96
Enzyme
Q7M558
350347
8.68
Enzyme
BAD04856 (Q76B34)
Reverse transcriptase
132804
Enzyme
14
Q7T040
90214
7.54
Enzyme
10
Q8UW40
ST7 protein
58059
7.03
Enzyme
14
Q6DR47
178147
8.93
Enzyme
2,5,8,10,11,
12, 13, 14, 15
Q76BE1
25178
6.00
Enzyme
11
Q7T315
Triosephospahte isomerase 1b
27100
6.90
Enzyme
11,12
Q90XF8
Triosephosphate isomerase B
26476
7.60
Enzyme
12
Q9PWD1
129986
8.38
Enzyme
7,9
Q7ZU23
42304
5.23
Structural
Q6TNW2
Actinin, alpha 2.
104086
5.23
Structural
Q6DHS1
42374
5.23
Structural
SWISS-PROT
Accession number
Q90333
Q6QUR3
Q7T2J3
Q76BG1
Protein Name
29
Mw
pI
Function
Band number
4.40
8.39
6.44
8.09
Structural
Structural
Structural
Enzyme
10
9
2
9
8.42
Enzyme
8,9,10
7.23
Enzyme
10
7.74
8.63
Enzyme
Enzyme
10
10
8.54
Enzyme
10
8.09
Enzyme
10
6.67
Enzyme
7.31
Enzyme
10
6.49
Enzyme
10
6.67
Enzyme
10
6.91
Enzyme
10
6.44
6.32
6.98
6.44
6.82
7.68
6.82
7.85
10.47
10.47
8.03
7.78
8.10
9.07
9.16
5.99
9.01
8.45
5.09
6.88
5.61
5.44
5.32
Enzyme
Enzyme
Enzyme
Enzyme
Enzyme
EnzEnzymeyme
Enzyme
Enzyme
Ribosomal
Ribosomal
Transcription factor
Transcription factor
Transport
Calcium ion binding
Translation Factor
Translation Factor
DNA-RNA -binding
Signal transduction
Hypothetical protein
Hypothetical protein
Hypothetical protein
Hypothetical protein
Hypothetical protein
Hypothetical protein
2
9
9
1,7,9
15
15
5
5
10
10
9
13
5
5
7
7
7
10
7
5
9
7
12
9
6.47
Hypothetical protein
30
Figure 4: Three dimensional densitometric analysis of SDSPAGE from Figure 3. Traces from 1 - 14 represent
the different protein lanes in SDS-PAGE. Peaks
correspond to bands of SDS-PAGE. Trace M
represents protein markers.
25
20
Conclusion
The protein profiles of snakehead fish of different
sizes that were caught in different months of the year
were compared. The results showed that all the fishes
have similar protein profiles, where each protein band
consisted of identical proteins. Furthermore, the relative
intensity of protein bands of all the fishes analyzed is
also similar. In view of high demands of snakehead fish,
culturing of the fish is the only solution. The present
data can be used as a reference for obtaining cultured
snakehead fish most similar in fish muscle protein
composition to the wild type fish.
15
Acknowledgements
10
5
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Ki
na
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Number of Identified
Proteins
30
31
Enzymes
32
References
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