Академический Документы
Профессиональный Документы
Культура Документы
METHODOLOGY:
100 extracted human single rooted teeth will be selected. All teeth will be
thoroughly cleaned and washed and preserved in thymol solution until further
use. All teeth will be analysed by digital radiography in the buccal and proximal
directions to confirm noncomplicated root canal anatomy, single straight root
canals, and mature root formation. The crowns will be sectioned with a diamond
disk and the root length will be standardized to approximately 16mm.
Root canals will be instrumented 0.5mm beyond the apical foramen up to size
30 or size 40 k-files depending on the experimental group. Irrigation will be
perform with 2ml of physiologic saline solution. The root surface will be then
coated with an epoxy resin except the cervical access and apical foramen. After
setting of epoxy resin, the canals will be fill with EDTA for 3 min in order to
remove the smear layer. This will be followed by 5ml of physiologic saline
SUBGROUP 1- NaOCl
SUBGROUP 2- Smear OFF 2-in-1
(EDTA+CHX)
SUBGROUP 3- CHX-Plus
GROUP 1
Irrigants
GROUP 2
Intra-canal
Medicaments
SUBGROUP 4- Ca(OH)2
SUBGROUP 5- Nisin
SUBGROUP 6- Propolis
GROUP 3
Combination
GROUP 4
Control
STATISTICAL ANALYSIS:
The amount of extruded debris will be analysed using different statistical
methods.
REVIEW OF LITERATURE:
Menezes, Valera et al (2004) conducted An in vitro study evaluated the
effectiveness of irrigants and intracanal medicaments on micro-organisms
within root canals. This study revealed that Ca(OH)2 + CMCP was the
most effective intracanal medicament. 2% CHX solution was more
effective than 2.5% NaOCl against E. Faecalis.
Edgar, Klaus et al. (2005) conducted An in-vitro study compared the
antimicobial efficacy of CHX and Two Ca(OH)2 formulations against E.
Faecalis. This study showed that CHX was significantly more effective
than Ca(OH)2 paste or a mixture of CHX + Ca(OH)2 paste .
Oncag O, Dilash, Uzel et al.(2006) conductedAn in-vitro study compared
the antibacterial efficacy of three commonly used intracanal medicaments
calcium hydroxide, chlorhexidine gel and Vitapex with propolis against
Enterococcus faecalis. This study revealed that propolis had better
antibacterial activity against E.faecalis in the root canals suggesting that
it would be used as an alternative medicament.
Zhongchun, Lin et al (2011) conductedAn in vitro study evaluated the
antibacterial activities of MTAD in combination with Nisin against E.
Faecalis. This study revealed that MTAD in conjunction with Nisin
inhibits E. Faecalis better than MTAD alone.
Suneel, Ashok et al. (2011)conductedAn in vitro study investigated the
newer intracanal medicament Nisin on E. Faecalis in comparison with
CHX and Ca(OH)2. this study showed that Nisin was more effective in
eradicating E. Faecalis cell in pure culture and root canal dentin.
Manavalan, Narasimhan et al. (2011) conductedAn in vitro study
evaluated Propolis and Triantibiotic Mixture as an intracanal
medicaments against E. Faecalis. This study showed that Propolis was
more effective than TAM against E. Faecalis at a 2 day time period and
both were equally effective at 7 days.
REFERENCE:
1. Menezes, Valera et al. An in vitro evaluation the effectiveness of
irrigants and intracanal medicaments on micro-organisms within root
canals. IEJ 37, 311-319, 2004
2. Edgar, Klaus et al. Antimicobial efficacy of CHX and Two Ca(OH)2
formulations against E. Faecalis. JOE Vol31, No.1 Jan 2005
3. Oncag O, Dilash, Uzel A. Efficacy of propolis as an intracanal
medicament against Enterococcus faecalis. Gen dent 2006;10:319-322
4. Zhongchun, Lin et al. An in vitro evaluation the antibacterial activities
of MTAD in combination with Nisin against E. Faecalis. JOE- Vol37,
no.8 , Aug 2011
5. Suneel, Ashok et al. An in vitro investigation the newer intracanal
medicament Nisin on E. Faecalis in comparison with CHX and Ca(OH)2.
Journal of the International Clinical Dental Research Organization/ JanApr 2011, vol 3, Issue 1.
6. Manavalan, Narasimhan et al. Comparativeevaluation of Propolis and
Triantibiotic Mixture as an intracanal medicaments against E. Faecalis.
JOE-Vol 37, No. 9, Sep 2011