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Cristina Hlevca
National Institute For Chemical-Pharmaceutical Research and
Development - ICCF Bucharest
Bucharest, Romania
elastic modulus. Also, it has a high surface area per unit mass,
feature combined with its highly hydrophilic nature, confers a
high liquid loading capacity [1].
The above listed properties make BC an attractive
biomaterial as scaffold material for tissue regeneration or
replacement, wound dressings, artificial skin, film coating
agent and matrix for drug loading and drug release [2-5].
A method to improve further more the remarkable features
of BC is through combination of bacterial cellulose with other
polymers (poly(vinyl alcohol) [6], poly(ethylene glycol) [7],
alginate [8], starch [9, 10], chitosan [11, 12]) or with
inorganic materials (hydroxyapatite [13]).
Several preparation methods of BC based composites were
reported: direct addition of polymer into BC culture medium
[14], mixing BC powder or BC ground with polymer solution
[15] and impregnation method by immersing BC membranes
in polymer solution [7]. Although, in present, there are a
considerable number of articles about the preparation and
characterization of various BC composites for biomedical
applications, only a small number of papers related to drug
delivery have been published [16,17].
The aim of this paper was to investigate the use of BC
composites for drug release. In this purpose multilayer
composites BC-Glycerol- Poly(vinyl alcohol) were prepared
and in vitro drug release studies were performed to evaluate
their overall potential in drug delivery applications.
Poly(vinyl alcohol) (PVA) materials present also desirable
characteristics for drug delivery applications, such as: high
degree of swelling, simple chemical structure, elastic nature,
non-toxic, non-carcinogenic, and bio-adhesive properties.
Amoxicillin, an antibiotic with high solubility in water (3.4
mg/mL) and commonly used against a broad-spectrum of
Gram-positive and Gram-negative microorganisms, was
chosen as model drug.
To our best knowledge, this is the first time that a
multilayer composite system based on BC-Glycerol and PVA
prepared in this manner was investigated for drug release
studies.
I.
INTRODUCTION
[I.
A. Materials
Amoxicillin, G[ycero[, HexadecyItrimethy[ ammonium
bromide (CTABr, chemical formula: CI9H4zNBr), PVA
(average Mr
145000 Da, 99 mole % hydrolyzed) were
purchased from Sigma Aldrich. All chemicals and reagents
used were of analytical grades.
BC membranes (99% water content) were produced in the
Microbiology
Laboratory
of
Chemical
Engineering
Department (UPB).
=
HO
TABLE I
EXPERIMENTAL PART
-oJY,)=t'x
}o
OH
SAMPLE COMPOSITION
Composition
---
Sample name
Glycerol
CTABr
mM
Al
0.5
0.5
A2
1.0
0.5
A3
0.5
0.75
A4
1.0
0.75
Bl
0.5
0.5
B2
1.0
0.5
B3
0.5
0.75
B4
1.0
0.75
III.
PYA
RESULTS
10.8
?i
0.6
0.5
::E 0.4
0.3
Model parameters
+-
L-
0.7
TABLE II
0.9
_A4
_84
0.2
0.1
o.
2000
6000
4000
time(s)
8000
0.9
0.8
?i
1=-
0.7
0.6
O.S
::E 0.4
0.3
0.2
0.1
_A1
2000
-0,2
'8
:;;
Cl
.Q
-0,3
7,6
4000
6000
time(s)
8,2
7,8
8000
8,4
A3
Linear(A3)
-0,7
-0,9
A2
-Linear(A2)
-0,6
-0,8
A1
-Linear(A1)
-0,4
-0,5
A4
-Linear(A4)
.6-
-1
log (t)
714
-0,2
'8
:;;
.,
Cl
.Q
7,6
-----, -----,
7,8
-0,4
-0,6
k'102
R2
Al
A2
A3
A4
0.33
0.45
0.63
0.56
4.89
1.65
0.37
0.65
0.99
0.93
0.95
0.96
Bl
Model
constant
Correlation
coefficient
0.28
6.03
0.94
B2
0.22
12.08
0.98
B3
0.47
1.48
0.94
B4
0.71
0.15
0.95
Diflusion
exponent
.01
rl---
_0,1
Sample
name
.6-
.6-
B1
-Linear(B1)
+
B2
-Linear(B2)
B3
-0,8
Linear(B3)
A
-1
B4
-Linear(B4)
-1,2
-1,4
log (t)
MtlMoo =kt,
(1)
IV.
CONCLUSION
[12]
[ 13]
[ 14]
[ 15]
[16]
[ 17]
[ 18]
[ 19]