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Keywords
Blanching, chemical composition,
fermentation, Uchakoro, vegetables,
Vitex doniana
Correspondence
Gabriel I. Okafor, Department of Food
Science and Technology, Faculty of
Agriculture, University of Nigeria,
Nsukka, Enugu, Nigeria.
Tel: +234-8023423908; Fax: +234-8030914952;
E-mail: gabriel.okafor@unn.edu.ng
Funding Information
The authors gratefully acknowledge the
parents of Osum Frances Chief and Mrs. C.
E. Osum who funded this research study.
Received: 20 October 2012; Revised: 18
February 2013; Accepted: 18 February 2013
Abstract
Fresh leaves of Vitex doniana were subjected to different food processing methods such as drying, blanching and drying, fermentation of the leaf extract as
well as blanching and fermentation of the leaf extract. The proximate composition, mineral and vitamin contents of the fresh and processed leaves were subsequently determined, using standard methods. The results shows that
V. doniana leaf and products had the following ranges of proximate parameters:
0.0717.29% protein, 1.856.33% fiber, 0.476.55% ash, 10.8695.67%
moisture, 0.051.29% fat, and 3.6158.08% carbohydrate. The level of micronutrients detected were generally high and ranged as follows: minerals Ca
(13.3859.50 mg/100 g), Fe (3.018.00 mg/100 g), Na (0.371.29 mg/100 g),
and vitamins C (1.532.98 mg/100 g), A (54.63583.26 IU), E (3.1153.36 mg/
100 g), and B2 (0.019.63 mg/100 g). However, the various processing methods
used led to significant (P < 0.05) decrease of micronutrients in the extracts,
while it caused significant (P < 0.05) increase in the protein, fat, and ash content of the oven-dried sample.
Practical Applications
This article evaluates the chemical composition (proximate, vitamin and mineral contents) of Vitex doniana
leaf and leaf products. Results of this study will help to
promote utilization and consumption of V. Doniana,
which is an underutilized plant with great nutritional
potentials.
Introduction
Vegetables, especially leafy vegetables are an important
source of vitamins, minerals, fibers, and some essential
amino acids (Oyenuga and Fetuga 1975; Grubben 1976;
Ihekoronye and Ngoddy 1985). They have high content of
water and abundance of cellulose. The cellulose is in a form
which although not digested, serves a useful purpose in the
intestine as roughages, thus promoting normal elimination
of waste products. Vegetables are known to contribute
2013 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of
the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,
provided the original work is properly cited.
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F. I. Osum et al.
Sample 1
Steam blanching, oven drying, milling of the dried leaves,
and packaging.
Two hundred grams (200 g) of the leaves was steam
blanched at 100C for 8 min. After blanching, the sample
was drained, dried at 50C for 24 h, milled, and packaged
in a moisture-proof nylon bag.
Sample 2
Oven drying, milling of the dried leaves, and packaging.
The second portion (200 g) of the leaves was oven dried
at 50C for 24 h, milled to obtain dried leaf powder. (This
sample was used as control.)
Sample 3
Steam blanching, wet milling, filtration, pasteurization,
and packaging.
The third portion weighing 200 g was steam blanched
at 100C for 10 min. After blanching, the sample was wet
milled using 250 mL of water (2:2.5) in a Kenwood Blender-BL300/BL350 and filtered using a sterilized muslin
cloth. After filtration, the sample was fermented at room
temperature (28 2C) for a period of 5 days and the
extract was pasteurized at 70C for 30 mins.
Sample 4
Fermentation, wet milling, filtration, pasteurization, and
packaging.
The fourth portion weighing 250 g was fermented at
room temperature (28 2C) for 5 days. Thereafter, the
sample was wet milled using 250 mL of water (2:2.5) in a
Kenwood Blender-BL300/BL350 series and filtered to separate the juice using a sterilized muslin cloth. The extract
was bottled and pasteurized at 70C for 30 min.
Sample 5
Raw extract, the raw unfermented extract which served as
control, was produced by wet milling the leaves using
250 mL of water (2:2.5) in a Kenwood Blender-BL300/
BL350 series, filtered using a sterilized muslin cloth, bottled, and pasteurized at 70C for 30 mins.
Sample analyses
Proximate (protein, crude fibre, ash, moisture, fat, and
carbohydrate), vitamins C and E contents were analyzed
according to Pearson (1976) methods. Vitamin A content
2013 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
F. I. Osum et al.
Statistical analysis
Data collected were subjected to one-way analysis of variance (ANOVA) using SPSS package version 17.0. Means
were separated using Duncans New Multiple Range test
(Duncan 1975). A difference was considered to be significant at P < 0.05.
Sodium
Raw leaf
Oven-dried leaf
Blanched & oven
dried
Blanched extract
Fermented leaf
extract
Blanched &
fermented extract
Calcium
Iron
1.29 0.03
1.25 0.01a
0.94 0.05b
51.70 0.03
59.50 0.02b
17.29 0.02c
17.29 0.02a
18.00 0.01a
7.84 0.01b
0.37 0.01d
0.60 0.01c
13.38 0.03d
14.33 0.04d
3.00 0.17c
3.50 0.00c
0.57 0.01c
14.30 0.01d
3.40 0.04c
Values are mean SD (n = 3). Values within the same column with
different superscript are significantly P < 0.05 different.
Table 1. Effect of processing methods on the proximate composition (%) of Uchakoro leaf and its extracts.
Treatments
Protein
Raw leaf
Oven-dried leaf
Blanched & oven dried
Blanched leaf extract
Fermented leaf extract
Blanched & fermented leaf extract
5.85
17.29
16.89
0.09
0.03
0.07
0.03c
0.01a
0.06b
0.01d
0.01d
0.01d
Fiber
Ash
1.85 0.01b
6.29 0.08a
6.33 0.04a
Trace
Trace
Trace
3.28
6.42
6.55
0.58
0.47
0.47
Moisture
0.01c
0.04b
0.01a
0.01d
0.01c
0.01c
75.00
11.25
10.86
95.67
93.78
93.99
Fat
0.7c
0.54d
0.16e
0.01a
0.01b
0.01b
1.10
1.25
1.29
0.05
0.14
0.15
CHO
0.01a
0.01a
0.04a
0.01c
0.00b
0.00b
12.92
57.50
58.08
3.61
5.58
5.32
0.03b
0.22a
0.08a
0.06e
0.03c
0.06d
Values are mean SD (n = 3). Values within the same column with different superscript are significantly P < 0.05 different.
CHO, carbohydrate.
2013 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
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F. I. Osum et al.
Table 3. Effect of processing methods on the vitamin composition of Vitex doniana leaf and leaf extract.
Treatment
Vitamin A IU
Raw leaf
Oven-dried leaf
Blanched & oven dried
Blanched extract
Fermented leaf extract
Blanched & fermented extract
3583.26
3572.30
3569.68
54.60
55.54
55.50
4.68a
3.52b
2.56b
0.14c
0.64c
0.28c
Vitamin B2 (mg/100 g)
Vitamin E (mg/100 g)
9.13
9.13
9.06
Trace
0.01
Trace
53.36
53.20
53.15
5.00
3.14
3.11
0.00a
0.00a
0.06a
0.00b
0.03a
0.04b
0.04b
0.03c
0.04d
0.03d
Vitamin C (mg/100 g)
32.98
20.83
18.94
2.16
1.56
1.52
0.07a
0.78b
0.01b
0.01c
0.37c
0.03c
Values are mean SD (n = 3). Values within the same column with different superscript are significantly P < 0.05 different.
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Conclusion
The leaf of V. doniana has very high nutrient and mineral
potentials; however, the processing treatments caused significant (P < 0.05) decrease in some of the nutrients.
Acknowledgment
The authors gratefully acknowledge the parents of Osum
Frances Chief and Mrs. C. E. Osum who funded this
research study. Also, we thank all who have contributed
in one way or the other to make this study a success.
Conflict of Interest
None declared.
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2013 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
F. I. Osum et al.
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