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Experimental Research or

Metaanalysis

PENSAR EN MOVIMIENTO:
Revista de Ciencias del Ejercicio y la Salud
EISSN: 1659-4436
Vol. 12, No. 2, pp. 16-30
Closing on December 31, 2014

STRUCTURED EXERCISE PROGRAM IS FEASIBLE AND IMPROVES FUNCTIONAL


CAPACITY AMONG OLDER ADULTS IN PUERTO RICO
Osvaldo J Hernandez Soto
ojhsui@gmail.com
Universidad Interamericana de Puerto Rico
Farah A Ramirez Marrero
farah.ramirez1@upr.edu
Universidad de Puerto Rico
Original submission: 13/09/2013; resubmitted: 28/5/2014, 05/07/2014; accepted: 06/08/2014;
published: 03/09/2014.
Also available in Spanish in this same journal. Submitted and reviewed in Spanish.
doi: http://dx.doi.org/10.15517/pensarmov.v12i2.16784

Abstract
Hernandez, O. J. & Ramirez, F. A. (2014). Structured exercise program is feasible and improves
functional capacity among older adults in Puerto Rico. PENSAR EN MOVIMIENTO: Revista de
Ciencias del Ejercicio y la Salud, 12 (2), 1-15. Physical inactivity is a major risk factor
affecting overall health and functional capacity among older adults. In this study we evaluated
functional capacity in 22 older adults in Puerto Rico (mean age standard deviation = 73.3
8.2 years) before, during and after eight weeks participation in a structured exercise program.
Functional capacity was evaluated using a field test battery (body composition, flexibility,
coordination, agility and balance, muscle endurance and cardiorespiratory endurance) validated
for this population. Also, cardiorespiratory fitness (VO2max) and blood lipid levels were
evaluated in a sub-sample (n = 7). A repeated measures ANOVA was used to detect changes in
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Structured exercise for older adults

functional capacity before, during and after the exercise program. A paired t-test was used to
evaluate changes in VO2max and lipids before and after the program. Flexibility improved
significantly during the exercise program (51.6 12.2 vs. 57.7 8.1 cm, p=0.04) and this
change was sustained at the end of the program (54.4 10.2 cm). At eight weeks into the
program, time in the agility and balance test improved by two seconds and muscle endurance
improved by five repetitions (p<0.05 for all). No changes were observed in body composition,
coordination, VO2max and lipid levels (p>0.05). These results suggest that participation in a
structured exercise program for eight weeks can positively impact factors that improve
movement capacity in older adults.
Key words: Physical Fitness; Ageing; Feasibility; Programmed Exercise
Resumen
Hernandez, O. J. & Ramirez, F. A. (2014). Programa de ejercicio estructurado es viable y
mejora la capacidad funcional en adultos mayores en Puerto Rico. PENSAR EN MOVIMIENTO:
Revista de Ciencias del Ejercicio y la Salud, 12 (2), 1-15. La inactividad fsica es un factor de
riesgo principal que afecta la salud y capacidad funcional en adultos mayores. En este estudio
se evalu la capacidad funcional en 22 adultos mayores en Puerto Rico (edad promedio
desviacin estndar = 73,3 8,2 aos) antes, durante y despus de ocho semanas de
participar en un programa de ejercicios estructurados. La capacidad funcional se evalu
mediante una batera de pruebas de campo (composicin corporal, flexibilidad, coordinacin,
agilidad y balance, tolerancia muscular y tolerancia cardiorrespiratoria) validadas para esta
poblacin. Adems, en una sub-muestra (n = 7) se midi la aptitud cardiorrespiratoria
(VO2max) y los niveles de lpidos en sangre. Se utiliz una ANOVA con medidas repetidas para
detectar cambios en la capacidad funcional antes, durante y despus del programa. Con una tpareada se evaluaron cambios en VO2max y lpidos antes y despus del programa. La
flexibilidad mejor significativamente durante el programa (51,6 12,2 vs. 57,7 8,1 cm,
p=0,04) y el cambio se sostuvo al final del programa (54,4 10,2 cm). El tiempo en la prueba
de agilidad y balance mejor por dos segundos y la tolerancia muscular aument por cinco
repeticiones al cabo de ocho semanas (p<0,05 en todos). No hubo cambios en la composicin
corporal, coordinacin, VO2max y nivel de lpidos (p>0,05). Estos resultados sugieren que la
participacin en un programa de ejercicios estructurados por ocho semanas puede impactar
positivamente aspectos que promueven la capacidad de movimiento en el adulto mayor.
Palabras clave: Aptitud fsica, Envejecimiento, Viabilidad, Ejercicio programado

One of the major challenges in public health nowadays is maintaining optimal health
status, independence and mobility in the growing population of older adults (CDC, 2003). This is
explained by the biological changes associated with the ageing process that increase the risk of
chronic diseases and disability. However, some of these biological changes like the reduced
cardiorespiratory fitness (VO2max), increased cholesterol, glucose intolerance, loss of calcium
and reduced lean body mass, are likely produced by the combined effect of ageing and the
physical inactivity and sedentary behaviors characterizing the older adult population (ChodzkoZajko et al., 2009).
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Structured exercise for older adults

Physical exercise programs for older adults are very limited and mostly inaccessible in
Puerto Rico (PR) and other countries around the World (Lopez, 2001; Rikli & Jones, 2013;
Romero-Arenas, Martinez-Pascual, & Alcaraz, 2013). Available programs are mostly focused on
very low intensity recreational activities (Lopez, 2001; Romero-Arenas et al., 2013). Some
studies have reported a 50-73 % participation in exercise interventions for older adults
(Leszczak, Olson, Stafford, & Brezzo, 2013; Rikli & Jones, 2013). The feasibility of a structured
exercise program for older adults in PR and its effect on functional capacity has not been
documented. Therefore, in this study we tested the following hypotheses: 1) at least 75 % of
participating older adults will complete the structured exercise program; 2) the exercise program
will improve functional capacity, cardiorespiratory fitness and blood lipid levels.
Methodology
A cuasi experimental design was used consisting of one group measured before, during
and after a structured exercise intervention (during = at week four, and after = at week eight of
the intervention).
Selection of Study Sample.
A convenience sample was selected among participants in the Hope for the Elder
community-based program in San Germn, PR. After being authorized by the program board of
directors and the Institutional Review Board (IRB) of the Interamerican University of Puerto
Rico, a meeting was conducted with the program directors and potential participants to explain
the study purpose and protocol including the scientifically structured exercise program (CSEP)
and the evaluations before, during and after the program. Inclusion (older adults at least 60
years of age, medical evaluation and authorization to participate in the exercise program) and
exclusion (diagnosed neuromuscular or neurocognitive disease, uncontrolled rheumatic
disease, anemia, recent embolism, uncontrolled metabolic disorders, acute and recurrent
diseases, active infections and constant fever, illicit drug use or use of medications altering
heart rate response) criteria were also explained.
Medical evaluations and authorization to participate in the study were obtained by a
family physician and a cardiologist in the community-based program. Medical evaluations
included presence of chronic diseases, physical activity level, general and cardiovascular health
status, tobacco and alcohol use. A resting electrocardiogram was also obtained and evaluated
by the cardiologist. From a total of 30 older adults evaluated, 27 received medical release to
participate in the study and later completed the informed consent form when all their questions
were answered by study personnel. Of these 27 participants, 22 (males = 3, females = 19)
completed all evaluations one week before, during (at week four) and one week after the
program (after week eight), and were included in the analyses.
The evaluations before starting the CSEP included anthropometric measurements
(weight, height, and thigh and biceps circumferences) and skinfold measures (chest, abdomen
and thigh for men; triceps, thigh and suprailiac for women). These measurements were used to
estimate percent body fat (Crombie et al., 2004). Resting blood pressure and heart rate (HR)
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Structured exercise for older adults

were also measured. Resting HR was used to obtain the target exercise HR using a percentage
of the HR reserve (Crombie et a., 2004).
Of the 22 participants, a sub-sample of 9 participants was randomly selected for
additional measurements, seven of which (women= 6, men=1) completed all measurements
that included cardiorespiratory (VO2max) and blood lipid levels (HDL and LDL) before and after
the exercise intervention. Before starting the CSEP, familiarization sessions were conducted
where a HR monitor was included. The CSEP consisted of three weekly sessions from 8:30 to
10:30 a.m.: dancing on Mondays, muscle strengthening exercises on Wednesdays, and walking
on Fridays.
Functional Capacity.
Functional capacity was evaluated with a battery of tests (Osness et al., 1996) validated among
older adults in PR (Luhring, 1996). These tests are designed to identify parameters associated
with functionality among older adults such as body composition, flexibility, agility and balance,
coordination, muscle endurance and cardiorespiratory endurance. Evaluations were conducted
in the morning and divided in two days: cardiorespiratory endurance the first day and the rest of
the tests the second day. All tests were administered on three occasions: before starting, during
(at week four), and after (after week eight) the CSEP.
Body Composition. Pondered index, a measure based on body weight and height
(height/3 x weight) was used. Body weight was measured with light clothing and no shoes or
socks. Height was measured with a metric tape vertically attached to a wall. After removing
shoes and socks, each participant stood looking forward with their back against the wall. A ruler
was placed horizontally over their head to register their height. Body mass index was also
determined (BMI: kg/m2).
Flexibility. With legs extended on the floor, feet apart by 30.5 cm and a 91.4 cm ruler
placed on the floor at toe level between the legs, each participant placed one hand over the
other and flexed their trunk sliding forward over the ruler sustaining the final position for at least
two seconds. The highest distance reached in three attempts was registered.
Coordination. On a table top, at 12.7 cm of one end, a 76.2 cm long line was marked
and over that line six 7.6 cm perpendicular lines were marked separated one from the other by a
12.7 cm distance. Three unopened soda cans of 341 ml were placed over perpendicular lines
one, three and five. The test consisted of recording the time it took for each participant to turn all
three cans and place them on the next perpendicular line (line tow, four and six), and then
return them to the original position. Two practice attempts were allowed before the two trials
from which the shortest time was recorded.
Agility and Dynamic Balance. From a sitting position on a chair with hand rests and
both toes on the floor, the test consisted of timing from the moment each participant stood up,
walked around the right side of a cone placed 182.9 cm from the chair, returned to the seat, sat
down, stood up again to walk around the left side of another cone, and returned again to the
seat and sat down. Each participant was instructed to complete the test as fast as possible and
to use the hand rests to keep balance and prevent falls. The shortest time from two trials was
recorded.
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Structured exercise for older adults

Muscle Endurance. From a sitting position on a chair without hand rests, both toes on
the floor and looking forward, each participant held a light weight (3.6 kg for men and 1.8 kg for
women) with their dominant hand, arm hanging on the side with their hand supine while the nondominant hand rested over the thigh. The test consisted of completing as many elbow flexions
and extensions as possible in 30 seconds. A zero was recorded when participants could not flex
their elbow.
Cardiorespiratory Endurance. The time it took for each participant to walk 800 meters
as fast as possible in a 400 meter track was recorded.
Cardiorespiratory Fitness (VO2max) and Lipids. Before starting the eight week
CSEP, a sub-sample of seven participants completed two additional tests: cardiorespiratory
fitness (VO2max) and blood lipid levels (total cholesterol, HDL, and LDL). The cardiorespiratory
fitness test was administered by an exercise physiologist in the Center for Sports Health and
Exercise Sciences at the Olympic Center in Salinas, PR. The test was conducted on a treadmill
using the modified Bruce protocol (Heyward, 2006).
A medical technologist took all blood samples for the lipid profile test by electrophoresis
(Spectrophotometer-Hitachi 704). All samples were analyzed in the Porta Coeli Clinical
Laboratory in San Germn, PR.
CSEP
The scientifically structured exercise program was conducted from 8:30 a. m. to 10:30 a.
m., three days per week during eight weeks. Dancing exercises were included on Mondays,
muscle strengthening exercises on Wednesdays, and walking on Fridays. Dancing exercises
included low impact aerobic dancing, popular music dancing, and ballroom dancing. Muscle
strengthening exercises included the use of elastic bands, hand weights of 0.5 to 0.9 kg,
medicinal and balance balls, and a variety of exercises on the floor and while standing or sitting.
Walking sessions were conducted on parks or athletic tracks nearby the community-based
program.
The intensity and duration of each activity was modified according to each participants
response. Exercise intensity started at 50 % of HR max and was increased by 5 % every two
weeks. Exercise duration started with 15 to 20 minutes and was increased by 5 % every four
weeks, with 20 to 30 minutes sessions by the end of the intervention. All CSEP included an
initial warm-up period (five to 10 minutes) and a final recovery session (10 to 15 minutes).
Warm-up and recovery sessions included five-to-ten meter walks, flexion and extension of
knees, hips, elbows, shoulders, and other body movements using a chair and a wall for support.
Relaxation exercises with classical music were also included at the end of the recovery period.
Statistical Analyses
Statistical analyses included measures of central tendency (mean) and variability
(standard deviation). The percentage of CSEP sessions completed was used to determine
attendance and feasibility of the program. Inferential analysis included a repeated measures
ANOVA to identify differences between measures before, during (at week four), and after the
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program (after week eight). A dependent t-test was used to detect differences in the VO2max
and lipid level measured in a sub-sample before and after completing the CSEP. An alpha of
0.05 was used to establish statistical significance and all statistical analyses were conducted
with Minitab 16 for Windows (Minitab Statistical Software, Minitab Inc. 2010, Pennsylvania,
U.S.).
Results
General Characteristics.
Participants age ranged between 60 and 93 years, with a mean standard deviation of 73.3
8.2 years. Body weight was 66.5 15.4 kg, height was 150.6 9.2 cm, and BMI was 29.2 6.0
kg/m2. The estimated percent body fat at the start of the study was 25.5 7.6 %. Resting HR
was 80.1 13.5 beats per minute, and resting blood pressure was 130/79 12/4 mmHg. The
average attendance to the CSEP sessions was 76 %.
Functional Capacity.
Table 1 shows the mean standard deviation (SD) of all variables of interest before, during (at
week four), and after the CSEP (after week eight): pondered index, flexibility, coordination,
agility and balance, muscle endurance, and cardiorespiratory endurance. A symbol is used to
identify values during and after the exercise program that are significantly different from the
values before the program (alpha < 0.05).
Seventy-five percent of participants reached values of 11.9 units or less in the pondered
index before starting the exercise program and these were not changed during or after the
program (p = 0.190). Mean BMI was maintained in the overweight category (25.1 to 29.9 kg/m 2)
in all evaluations (data not included in the table). Flexibility improved significantly during
(increased by 6.1 cm at week four), and after the program (increased by 3.8 cm between weeks
four and eight) (p = 0.003). Time in the agility and balance test and the number of repetitions in
the muscle endurance test improved significantly by week eight (p = 0.013 and p < 0.001,
respectively) compared with the results before starting the program. Coordination and
cardiorespiratory endurance did not change as a result of the CSEP (p = 0.286 and p = 0.217,
respectively).
VO2max and Blood Lipids.
VO2max in the sub-sample increased by 1.68 ml kg-1 min-1 from pre to post-testing
(21.6 5.1 to 22.9 8.0 ml kg-1 min-1), but the difference was not statistically significant (p =
0.23). Total cholesterol, HDL and LDL did not change significantly as a result of the CSEP
(191.0 18.4 to 184.5 27.1 mg/dL [p = 0.97], 56.3 15.7 to 50.5 16.2 mg/dL [p = 0.99], and
101.1 25.2 to 95.9 34.4 mg/dL [p = 0.43], respectively).

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Discussion
The most important findings of the present study are: 1) the feasibility of the CSEP was
shown with over 75 % attendance; and 2) the significant improvement in many functional
capacity criteria after eight weeks of participation in the CSEP for older adults in PR.
Improvement in flexibility as a result of the CSEP is one aspect that has been observed
in previous studies including physical activity and exercise for older adults. Performing flexibility
exercises at the beginning and end of each CSEP session could have influenced this result. For
example, it has been observed that older adults significantly improve their flexibility after
participating in aerobic exercise that included an initial stretching session (Clark, 1994) and also
a muscle resistance session out of the water (Roma et al., 2013). Also significant improvement
in flexibility has been shown among older adults after participating in a 12 month Tai Chi
program (Lan, Lai, Chen, & Wong, 1998), after participating in a 12 week aerobic exercise
program post hospitalization (Brovold, Skelton, & Bergland, 2013), or after participating in
functional exercises organized into circuits (Whitehurst, Johnson, Parker, Brown, & Ford, 2005).
Flexibility significantly improved at week four of the CSEP and the improvement was sustained
at week eight of the program, thus suggesting that the program was effective in causing
changes in flexibility. This is especially relevant given the fact that poor lower back and
hamstring flexibility is associated with chronic back pain, risk of falls and fractures, and reduced
capacity to engage in daily activities among older adults (Cedric & James, 1999; Gallon et al.,
2011; Lan, et al., 1998). It has also been suggested that good flexibility helps improve speed,
dynamic balance, and muscle endurance (Stanziano, Roos, Perry, Lai, & Signorile, 2009;
Thompson, 1991), all of them important to achieve good mobility.
The observed improvement in agility and balance supports other studies that have used
different exercise programs in older adults (Chodzko-Zajko et al., 2009; Chou, Hwang, & Wu,
2012; Kronhed, Mller, Olsson, & Mller, 2001). Studies that have evaluated exercise programs
for older adults which include walking, dancing, muscle endurance, strength and flexibility, and
also exercise programs in the water, have also reported improvements in posture stability and
balance (Chodzko-Zajko, et al., 2009; Kim & O'Sullivan, 2013). Improving and maintaining good
balance is important to prevent falls and fractures in the older population (Chodzko-Zajko et al.,
2009; Gillespie et al., 2003).
The significant improvement in muscle endurance observed in the present study also
supports other studies in which specific exercises for muscle strength were offered to older
adults (Brandon, Boyette, Gaash, & Lloyd, 2000; Mayer et al., 2011; ONeill, Thayer, Taylor,
Dzialozyuski, & Noble, 2000; Westhoff, Stemmerik, & Boushuizen, 2000). The CSEP in the
present study included one day for muscle strength and endurance activities suggesting that
general exercise programs can also stimulate muscle strength improvements among older
adults; and therefore, help improve or maintain factors associated with movement capacity such
as walking speed, standing from a chair, carrying objects, and walking up and down a stair
(Brandon, et al., 2000; Mian et al., 2007; Pinto et al., 2014).
Body composition was a criterion that did not change as a result of the CSEP in our
older adult participants. This aspect was different from other studies that have reported a
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reduction in body weight and body fat among older adults after participation in exercise
programs (Lee, Kim, & Oh, 2013; Meredith, Frontera, O'Reilly, & Evans, 1992; Romero-Arenas
et al., 2013; Tan, Li, & Wang, 2012; Toth, Beckett, & Poehlman, 1999). Two factors could
explain the lack of change in body composition observed in the present study: 1) the lack of
dietary control or dietary modification, or education about nutrition in the CSEP, and 2) the
volume and intensity of the physical activities included in the CSEP could have been insufficient
to provoke an energy deficit. However, it is also important to prevent unnecessary weight loss
among older adults that are associated with health complications, particularly among those that
are not obese (Soenen & Chapman, 2013). Participants in the present study did not increase
their body weight or BMI after eight weeks, a finding that could also be interpreted as positive.
The lack of change in coordination as a result of the CSEP differs from a previous study
(Bennett & Castiello, 1994) in which an important reduction in the time to transport and
manipulate objects was observed among older adults. In that study, Bennett y Castiello (1994)
argued that the reduction was possibly due to a lower emphasis on the accuracy and more
emphasis on the final result that was to complete the task as fast as possible. In the present
study, the test emphasis was on both the accuracy and the speed. Although the CSEP did not
include specific activities for motor coordination, the fact that this criterion did not decrease
could be the result of other manual activities included in the community-based program
(Ranganathan, Siemionow, Sahgal, & Yue, 2001). It has been suggested that coordination can
progressively improve by slowly but precisely engaging in simple movement patterns (Fujiyama,
Hinder, Garry, & Summers, 2013; Kottke, Halpern, Easton, Ozel, & Burrill, 1978) and that
participation in exercise programs helps prevent the loss of motor skills and coordination among
older adults (Heyward, 2006; Leszczak et al., 2013; Skelton, Greig, Davies, & Young, 1994;
Wong, Lin, Chou, Tang, & Wong, 2001).
Cardiorespiratory endurance as part of a battery of functional capacity tests could be
influenced by a good VO2max as well as by a good mechanical efficiency when completing a
distance as fast as possible. The former has a molecular and genetic component, while the
latter has a neuromuscular and perceptual component more directly related with motor capacity
(Joyner & Coyle, 2008). Improvements in cardiorespiratory endurance have been observed
during field tests (Mian, et al., 2007) and improvements in VO2max during laboratory tests
conducted with older adults after participating in exercise programs (Chodzko-Zajko et al., 2009;
Hagner, Hagner-Derengowska, Wiacek, & Zubrzycki, 2009). Although there was a tendency to
reduce the time during the 800 m test, the difference was not statistically significant and,
therefore, we concluded that cardiorespiratory endurance as a criterion of functional capacity
did not improve with participation in our CSEP. It is possible that the intensity of the activities
provided during the CSEP were not enough to provoke changes in this component. Similarly,
there was a tendency for the VO2max to improve but the difference was not significant. This
could also be explained by the small number of participants in the sub-sample.
High density lipoprotein (HDL) and low density lipoproteins (LDL) did not change
significantly as a result of the CSEP. This observation supports previous studies (Ready et al.,
1996; Wagner, La Croix, Buchner, & Larson, 1992), but differs from others (Fahlman, Boardley,
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Lambert, & Flynn, 2002; Gallon et al., 2011; Isler, Kosar, & Korkusuz, 2001; Kostka, Lacour,
Berthouze, & Bonnefoy, 1999) in which HDL improved as a result of an exercise intervention
among older adults. It is possible that exercise programs for this population require a higher
duration and higher intensity level in order to reach significant changes in these parameters.
There are several limitations in the present study worth discussing. First, the battery of
tests to evaluate physical fitness and functional capacity associated with older adults capacity
for an independent life (Rikli & Jones, 2013) was not considered and should be included in a
future study. However, the battery of tests selected for the present study is the only one that has
been validated in older adults in PR (Luhring, 1996) and the observed results were within those
reported by Luhring (1996). The lack of information regarding nutrition and energy expenditure,
and the control over the volume and intensity of the CSEP were two limitations that could
probably influence our results and should be considered for future studies. It is interesting to
highlight that these controls are not the norm in real life situations suggesting that exercise
programs like the CSEP provided in this study is still valuable for improving or maintaining
functional capacity parameters among older adults in PR. Finally, the sub-sample was probably
too small (n = 7) to be able to detect significant changes in VO 2max, and also the number of
male participants (n = 3) limited the capacity to establish differences by sex.
Another interesting evaluation of the present study was the feasibility and satisfaction of
the participants with the exercise program. Regular and on-time attendance, the effort to
complete all activities, and the rapport between participants and researchers all suggest that the
CSEP is feasible and acceptable to a group of older adults in PR. In conclusion, our results
suggest that the CSEP is not only feasible but also beneficial in improving functional capacity,
an essential aspect of mobility and general health among older adults. Programs such as the
one provided in the present study could help reduce sedentary behaviors and increase the
engagement of older adults in daily physical activities that improve independence and quality of
life.
Acknowledgements
A special thanks to the HOPE for the Elder in Puerto Rico community-based program
(Esperanza para la Vejez de Puerto Rico in Spanish); to Mrs. Carmen Flores, director of the
HOPE Center in San Germn; to Dr. Edgardo Lorenzo Gonzlez for the statistical analyses; to
the cardiologist, Dr. Radams Gonzlez for conducting all participants medical evaluations; to
Dr. Enrique Amy and Dr. Anita Rivera-Brown for the VO2max tests in the Exercise Physiology
Laboratory at the Olympic Center in PR; to Dr. Ilia Morales and professor Ramn lvarez who
assisted with the tests conducted in the Olympic Center; and to all instructors and graduate and
undergraduate students in the Physical Education Program at the Interamerican University of
Puerto Rico, San Germn Campus, for assisting with the administration of tests and the
exercise program.

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Table 1.
Mean and standard deviation (S.D.) of study variables before, during and after the exercise
program.
n

Pondered Index (units)


Flexibility (centimeters)
Agility and Balance (seconds)
Coordination (seconds)
Muscle Endurance (repetitions)
Cardiorespiratory Endurance (min)

22
22
22
22
22
22

Before

During
(4 weeks)

After
(8 weeks)

p (F)

Meana

S.D.

Mean

S.D.

Mean

S.D.

11.3
51.6
29.9
12.4
18.5
11.6

1.0
12.2
10.8
4.7
5.9
3.8

11.3
57.7*
29.8
11.1
23.4
11.2

0.8
8.1
10.5
4.8
5.2
3.7

11.5
54.4*
27.9*
11.8
23.9*
11.2

0.7
10.2
8.6
8.3
4.5
3.3

* p < 0.05 compared with pre (before) test, p < 0.05 compared with during test"

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0.190 (1.73)
0.003 (6.57)
0.013 (4.81)
0.286 (1.29)
<0.001 (20.67)
0.217 (1.35)

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