Clculos

CURVA DE CALIBRACIN
5000
4500

f(x) = 872x + 242.02

R = 0.99

4000
3500
3000
LONGUITUDES DE EMISION

2500
2000
1500
1000
500
0
0

mL DE RIBOFLAVINA

PATRON EXTERNO

x o=

y oa
b

0,67818930,21314645
x o=
=0,931165289 ppm
1
0,49940302 ppm

[Ecuacin 1]

x o 2 2 xo 2 2 x o 2 2
sx =
s +
s +
sb
yo y
a a
b
2

( ) ( ) ( )
o

[Ecuacin 2]

1 2
1 2
2
s x 2=
( 0,0001)2 +
(0,060683553)2+ (a ) (0,017921714)2
b
b
o

()

( )

s x =0,12 ppm
o

RSD x =13,06
o

0,931165289 mgriboflavina
50 mL
1000 mL
C Riboflavina=
100
5,3 mg

C Riboflavina=0,88
C Riboflavina=0,11 ppm

Error=

|Valor RealValor exp .|

100

Valor Real

[Ecuacin 3]

Error C

Riboflavina

|0,880,92|

Error C

100

0,92
Riboflavina

=4,35
LDD=3

0,057859497
=0,35
1
0,49940302 ppm

LIMITES DE DETECCION Y DE
CUANTIFICACION

LDC=10

0,057859497
=1,16
1
0,49940302 ppm