21/09/2016 DeterminationofbovineimmunoglobulinGinbovinecolostrumpowders,bovinemilkpowders,anddietarysupplementscontainingbovinecolostru

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JAOACInt.2013SepOct96(5):102632.

DeterminationofbovineimmunoglobulinGinbovinecolostrumpowders,
bovinemilkpowders,anddietarysupplementscontainingbovinecolostrum
productsbyanautomateddirectimmunoassaywithopticalbiosensor:
collaborativestudy.
GapperL1.

Authorinformation
Abstract
NinelaboratoriesparticipatedinanAOACcollaborativestudytodeterminebovineimmunoglobulinG
(IgG)levelsinselecteddairypowdersanddietarysupplementsbysurfaceplasmonresonance(SPR)
methodology.Eachsamplematrixwasdissolvedinbufferandsuitablydilutedtofitwithinthestandard
curve.Thesampleextractwasinjectedoverasurfacefunctionalizedwithaffinitypurified,polyclonal
goatantibovineIgG(H+L)antibodyIgGwasthendetected.SPRdetectionwasusedforthedirect
immunoassayandquantificationwasmadeagainstacalibrationcurvepreparedfrombovineserum
IgG.Betweeneachstandardandsample,thesurfacewasregeneratedusing10mMglycineatpH
1.5.Thesamplesanalyzedincludedthelikelymatrixesforwhichtheassaywouldfindcommercial
use,namely,highandlowproteincontentcolostrumpowders,tabletscontainingcolostrumpowder,
infantformulacontainingcolostrumpowder,andsomeIgGcontainingdairypowders,i.e.,milkprotein
isolate,wheyproteinconcentrate,andskimmilkpowder.Eachlaboratoryprovideddataforthestudy
andassayedblindduplicatesofsevenmaterials.Duetogrossoutliersinthemajorityofresultsfrom
onelaboratory,thedatafromeightlaboratorieswereusedforthestatisticalanalysis.Therepeatability
RSD(RSDr)valuesrangedfrom3.2to7.3%,andthereproducibilityRSDRvaluesfrom13.0to22.6%.
PMID:24282942
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21/09/2016 DeterminationofbovineimmunoglobulinGinbovinecolostrumpowders,bovinemilkpowders,anddietarysupplementscontainingbovinecolostru

http://www.ncbi.nlm.nih.gov/pubmed/24282942

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