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DOI 10.1007/s12011-012-9448-z
Received: 31 January 2012 / Accepted: 3 May 2012 / Published online: 15 May 2012
# The Author(s) 2012. This article is published with open access at Springerlink.com
tea extract group than in the control, although HDLcholesterol and magnesium were higher in the green tea
extract group than in the placebo group. At baseline, a
positive correlation was found between calcium and body
mass index, as was a negative correlation between copper
and triglycerides. After 3 months, a positive correlation
between iron and body mass index and between magnesium
and HDL-cholesterol, as well as a negative correlation between magnesium and glucose, were observed. The present
findings demonstrate that green tea influences the body's
mineral status. Moreover, the results of this study confirm
the beneficial effects of green tea extract supplementation on
body mass index, lipid profile, and total antioxidant status in
patients with obesity.
Keywords Obesity . Green tea . Polyphenol
epigallocatechin-3-gallate . Minerals . Oxidative stress .
Lipid . Glucose
Introduction
J. Suliburska (*)
Department of Human Nutrition and Hygiene,
Poznan University of Life Sciences,
Wojska Polskiego 31 Str.,
60-624, Poznan, Poland
e-mail: jsulibur@up.poznan.pl
P. Bogdanski : M. Szulinska : M. Stepien : D. Pupek-Musialik
Department of Internal Medicine, Metabolic Disorders and
Hypertension, Poznan University of Medical Sciences,
Poznan, Poland
A. Jablecka
Department of Clinical Pharmacology,
Poznan University of Medical Sciences,
Poznan, Poland
316
generated during aerobic metabolism, i.e., during mitochondrial and glycated protein oxidations. Blood may be vulnerable to oxidative stress induced by diabetes and may
become exposed to ROS continuously generated by the
auto-oxidation of hemoglobin and polyunsaturated fatty
acids (PUFAs) [4, 5]. Because of their high rate of oxygen
consumption, their high content of PUFAs, and their poor
enzymatic antioxidant defense, blood cells exhibit increased
vulnerability to diabetes-induced oxidative stress [4, 5].
Lipid peroxidation (LP) causes injury to cells and to intracellular membranes and may lead to cell damage and subsequently to cell death [6]. In order to scavenge ROS,
various enzymatic and nonenzymatic antioxidant defense
systems exist in the blood [7].
Moreover, many of the metabolic imbalances involved in
obesity (e.g., glycemic, lipidemic) give rise to organ dysfunction and accelerated aging processes [8]. Several studies
show obesity-related abnormalities in mineral status. The
association between overweight, obesity, Fe, calcium Ca,
and Zn deficiency has been observed in clinical and epidemiological studies [9]. Several studies suggest that Ca may
play a role in the regulation of abdominal fat mass in obese
people [10].
Understanding these pathological processes is crucial for
better prevention and treatment of patients with obesity. The
possible use of so-called functional food in this group of
patients is particularly intriguing.
In recent years, researchers throughout the world have
been investigating the potential benefits of green tea (GT)
and its most abundant catechin, polyphenol
epigallocatechin-3-gallate (EGCG). The positive effects of
GT supplementation have been observed particularly in the
prevention and control of type 2 diabetes [11]. In recent
years, there have been a number of trials in humans showing
the favorable effects of GT supplementation on body weight
and body composition [12]. GT also has positive effects in
the prevention and treatment of cardiovascular diseases
[13]. Human studies suggest that GT may contribute to a
reduction in the risk of atherosclerosis and cancer, as well as
to the promotion of anti-hypertensive effects, body-weight
control, antibacterial effects, increases in bone-mineral density, anti-fibrotic properties, and neuroprotective power [14].
There is growing evidence showing the possibility of using
GT in the prevention and treatment of obesity and coexisting
diseases [11, 15].
Several reports have focused on the interactions of
polyphenols with metal ions, e.g., Zn and Fe, and have
suggested their possible influence on mineral distribution in the body [16, 17]. There are few studies on the
effects of GT on mineral status. The small number of
studies evaluating green tea extract's (GTE) influence on
mineral status in obese patients have shown inconclusive results.
Suliburska et al.
The aim of our study was to estimate the influence of a 3month oral supplementation of GTE on serum concentrations of minerals, on body mass, on total antioxidant status,
on the lipid profile, and on glucose concentration in obese
patients.
317
Biochemical Measurements
Statistical Analysis
Ca
Mg
g/g
61.128.28 1098.04148.3
g/one capsule
23.223.42
416.2456.3
Fe
Zn
Cu
5.670.34
1.810.32
0.510.17
2.150.06
0.680.11
0.190.03
Results
Forty-six out of 145 obese patients with hypertension
screened at our outpatient clinic fulfilled the inclusion and
exclusion criteria and were allocated equally into the GTE
and placebo groups.
318
Suliburska et al.
Discussion
The significant influence demonstrated in our study of a
3-month GTE supplementation on mineral levels in obese
patients is a new finding. Moreover, the favorable effect of
GTE supplementation on body mass, lipid profile, glucose,
and antioxidant status was also demonstrated.
The potential of green tea as a natural agent of weight
loss has been investigated in some other studies similar to
our own [18, 19]. Some authors suggest that the polyphenolic components of green tea have an anti-obesogenic
effect on fat homeostasis, by increasing thermogenesis, reducing fat absorption, and introducing modifications in appetite [20, 21]. The results obtained (significant differences
in BMI between the GTE group and the placebo group, and
the slight reduction in BMI in the group with GTE) support
the possible role of green tea in weight loss.
A number of experimental studies have been conducted
to examine the effects of GT on carbohydrate metabolism
and lipid profile [15, 19, 22]. Wu et al. [15] demonstrated
that during 12-week GT supplementation, insulin sensitivity
in SpragueDawley rats increased. They concluded that the
most active component in GT is polyphenol.
Abnormalities of lipid metabolism are one of the major
risk factors for the development of cardiovascular disease,
very often associated with obesity. The outcomes of our
study show that a 3-month supplementation exerts a positive
effect on lipid profile in patients with obesity. Decreases in
TC, LDL, and TG concentration were observed, along with
increases in HDL concentration (within the study group).
The positive effect of GT in human studies has also been
observed by others [19, 23]. The results could be partly
caused by the decreased absorption of cholesterol and glucose from the diet in the presence of the polyphenols from
GTE in the small intestine, especially if the supplement is
taken by subjects during meals [24]. GT catechins influence
micellar solubility, luminal lipid hydrolysis, and intestinal
lipid absorption, and they may interfere with the emulsification, hydrolysis, and micellar solubilization of lipids, suggesting that the cholesterol-lowering effect of catechins
Age (years)
Time since diagnosis of obesity (years)
BMI (kg/m2)
Waist circumference (cm)
Placebo (n023)
12/11
52.267.71
8.352.85
33.452.65
104.986.35
p
NS
NS
NS
NS
NS
319
Table 3 Within-group anthropometric, blood pressure, and biochemical data at baseline and after 3 months of treatment
Variables
Baseline
BMI (kg/m2)
Waist circumference
(cm) SBP (mmHg)
DBP (mmHg)
LDL (mmol/L)
HDL (mmol/L)
Glucose (mmol/L)
TAS (mmol/L)
After 3 months
GTE group
(n023)
Placebo
(n023)
GTE group
(n023)
Placebo
(n023)
32.072.41
101.786.42
130.726.95
85.1012.47
33.452.65
104.986.53
129.587.88
84.213.32
NS
NS NS
33.362.66
105.026.10
128.326.16
84.503.86
0.03
0.04 NS
NS
31.712.29
101.156.42
128.156.86
84.0612.26
NS
NS
NS
NS
3.020.96*
1.280.26*
5.480.31*
2.090.28*
3.711.12
1.150.21
5.590.43
1.910.13
3.441.03
1.120.24
5.640.44
1.910.23
3.801.19
1.090.25
5.670.44
1.900.15
NS
0.02
NS
NS
<0.01
Data are mean SD; the arithmetic mean; SD standard deviation, n number of subjects; *p<0.05 from baseline to the end of treatment with paired t
test
GTE green tea extract, BMI body mass index, SBP systolic blood pressure, DBP diastolic blood pressure, LDL LDL cholesterol concentration, HDL
HDL cholesterol concentration, TAS total antioxidant status, NS not significant, p level of significance
b
*
a**
[mmol/l]
[mmol/l]
a**
*, **
*, **
a,b
a,b
significantly differences (p<0.05) within group from baseline to the end of treatment
significantly differences (p<0.01) between GTE and placebo groups
significantly differences (p<0.05) within group from baseline to the end of treatment
significantly differences (p<0.01) between GTE and placebo groups
320
Suliburska et al.
Table 4 Within-group minerals concentration in serum at baseline and after 3 months of treatment
Variables
Fe (mol/l)
Zn (mol/l)
Cu (mol/l)
Ca (mmol/l)
Mg (mmol/l)
Baseline
After 3 months
GTE group
(n023)
Placebo
(n023)
GTE group
(n023)
Placebo
(n023)
17.912.20
12.092.12
13.973.12
2.720.59
0.970.52
17.283.16
12.453.11
14.302.13
2.750.63
1.050.76
NS
NS
NS
NS
NS
16.023.19*
14.083.16*
13.822.98
2.920.92
1.070.68*
16.912.97
13.012.98
14.453.17
2.980.69
1.090.43
NS
<0.05
NS
NS
NS
Data are mean SD; the arithmetic mean; SD standard deviation, n number of subjects; *p<0.05 from baseline to the end of treatment with paired t
test
GTE green tea extract, NS not significant, p level of significance
induce an inflammatory state, and some studies have suggested that inflammation contributes to high serum Fe levels
in obese subjects [31]. In this study, the reduction in BMI
index was associated with a decrease in inflammation biomarkers (unpublished data) and in the serum Fe level.
As with other authors, we found that GT treatment reduced Fe status and improved Zn and Mg status in patients
and experimental animals [16, 17]. The increased Zn level in
the serum may be associated with improved antioxidant
status subsequent to GT treatment, as concluded by Hamdaoui et al. [16].
We have also observed an association between glucose
and Mg, which confirms the results of other studies [32, 33].
In this study, we observed following supplementation that
there were increased concentrations of Mg associated with
decreased levels of glucose in the serum of the GTE group.
It may be suggested that higher levels of Mg in the body led
to a reduction in plasma glucose and stabilized the glucose
level during treatment. Soltani et al. [34] concluded that Mg
could improve the structure of the pancreas (especially in
diabetic individuals) and may prevent morphological
changes of blood vessels. In this study, the improvement
in Mg status after GTE supplementation was associated with
higher levels of HDL cholesterol in the serum of patients. A
positive correlation between Mg in serum and HDL has also
Table 5 Significant
correlation between
minerals and other
parameters in GTE
group
Conclusions
r, Pearson's correlation
coefficient; p, level of
significance
Minerals
Parameters
Baseline
Ca
BMI
Cu
TG
After treatment
Fe
BMI
Mg
glucose
Mg
HDL
r; p
0.33; 0.042
0.34; 0.038
0.37; 0.023
0.48; 0.002
0.32; 0.023
321
14.
15.
16.
Conflict of Interest None
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution,
and reproduction in any medium, provided the original author(s) and
the source are credited.
17.
18.
19.
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