Академический Документы
Профессиональный Документы
Культура Документы
INTRODUCTION
Vegetables
and
fruits
are
horticultural commodities that contain
lots of vitamins and minerals. They
also have tremendous potential as a
source of income of farmers even
source
of
foreign
exchange.
Consumption of vegetables and fruits
are starting to increase, due to an
awareness that eating fruits and
vegetables mean a healthy life will
increase.
Buleleng regency is located in the
northern part of Bali, known as the
center of Bali's grape. Buleleng
regency has a potential as a center for
grapes production because grapes are
suitable cultivated in Buleleng territory,
especially in the lowlands (0-400 m
asl). The development center of
grapes are still opportunities in the
District of Banjar, Seririt and Gerokgak
to reach 1.500 Ha grapes regions.
Data of Statistics Central Department
of Buleleng regency from 2006 to
2008 shows the development of
grapes production increase from
15.873,00 ton become 22.125,00 ton
(Statistics Central Department of
Agriculture and Livestock Buleleng
regency, 2010).
Statistic Centrals Department of
Buleleng regency also records from
nine districts in Buleleng, there are
three districts as the center of grapes
production, namely Banjar district,
Seririt district and Gerokgak district.
Banjar district become the largest
supplier of grapes with grapes
production amounted to 6.379 tons in
2010. The grapes production in Banjar
village is highest compared with the
production of the other villages. The
production of grapes in 2008 shows in
Tampekan village produced 334 tons
of grapes, Banjar village produced
2043 tons of grapes, Banjar Tegehe
village produced 134 tons of grapes,
Dencarik village produced 1704 tons
RESEARCH METHOD
The pesticides contain in grape
was extended according to multiresidue analysis of pesticides in fruit
RESULT
using disque, a dispersive solid-phase
Based on the research has been
extraction kit procedure (Shia, J. C.,
conducted 15 gram of grapes are
Michael S. Y. and Diane M. D. 2008).
chopped into small portions, and the
Grapes sample are chopped into small
pulverized by a food blender until it
portions, and the pulverized by a food
reaches
homogeneous
mixture.
blender until it reaches homogeneous
Transfer 15 g of the homogeneous
mixture. Transfer 15 g of the
fruit sample into the extraction tube 1,
homogenized fruit sample into the
and then add 15 mL of 1% acetic acid
extraction tube 1, and then add 15 mL
in acetonitrile.
of 1% acetic acid in
acetonitrile. Shake
the tube vigorously
for 1 minute and
centrifuge at 1500 rcf
for
1
minute.
Transfer 1 mL of the
upper layer extract
from tube 1 to cleanup tube 2. Shake
tube 2 vigorously for
1
minute
and
centrifuge at 1500 rcf
at least 1 minute.
The extract obtained
was collected into
Erlenmeyer flask.
Cleanup process
of the extract was
performed
by
chromatography
Figure 1. Chromatogram of mixture
column using 10
of standard pesticide
grams preactivated
adsorbent silica gel
60 G (E. Merck. Art. 7734). Before
use, the columns were conditioned
with 20 mL of acetonitrile without
allowing them dry out. The column
was loaded with 10 mL grape extract
and eluted with 40 mL of acetonitrile.
Eluent was evaporated to dryness at
700C on the rotary evaporator. The
N
o
.
1
2
3
4
5
6
7
8
9
and
sample
C4
contains
of
hexaconazole
0.564
ppm
and
piperonyl butoxide 0.0017 ppm. Based
on BSN the levels of hexaconazole in
farmer group Amerta Nadi was
exceeded the allowable limit maximum
about 0.1 ppm and for the levels of
piperonyl
butoxide
was
under
maximum limit allowed about 2 ppm.
The pesticide residue that detected in
Amerta Nadi groups can be found in
pesticide trade mark such as in Anvil
and Cypermax that contain of
hexaconazole pesticide residue and
Bamex
that
contain
piperonyl
butoxide.
The calculation result of residue
pesticide contained in all grapes
samples in Banjar Village gives in
Table 1. From the Table 1 can be
known that hexaconazole as a residue
pesticide that can found in all
samples, it can be found in Anvil
fungicide. Anvil is systemic fungicide
with suspension shaped and brownish
white color used to controlling the
disease in rubber plant, soybeans,
coffee, watermelon, and grapes.
Excessive use of this pesticide can
cause poisoning through the eyes,
mouth, and skin. Symptoms of
poisoning
this
pesticide
are
characterized by loss of body balance,
dizziness, and nausea.
Chloropyrifos residue only found in
sample B (Sari Amerta), this
insecticide
found
in
Cypermax
insecticide. Cypermax is an insecticide
as a contact poison and stomach
poison with emulsified concentrates
shaped. This insectide used when
infestations occur by insect. Research
by the U.S. EPA in 1993 chlorpyrifos
was classified in Group E, evidence of
non-carcinogenicity for humans (U.S.
Environmental Protection Agency,
2005).
Piperonyl butoxide residue that only
found in sample C (Amerta Nadi), this
ACKNOWLEDGEMENT
We would like to deliver respectfully
thank for Forensik Laboratory Polresta
Denpasar for the helps in analysis
data of sample by using Gas
Chromatography-Mass Spectroscopy.
REFERENCES