C

log C (ng/km (%)

m (probit)
34 1.531479
0 #VALUE!
44 1.643453
0.25 -0.67449
64 1.80618
0.49 -0.025069
82 1.913814
0.78 0.772193
105 2.021189
0.93 1.475791
120 2.079181
1 #VALUE!

log C
M (%)
M (probit)
0
Err:502
Err:502
Err:502
1
0 6.80E-025 -10.2365
2 0.30103 9.14E-018 -8.504246
3 0.477121 3.42E-014 -7.490939
4 0.60206 6.35E-012 -6.771986
5 0.69897 2.58E-010 -6.214324
6 0.778151 4.24E-009 -5.75868
7 0.845098 3.86E-008 -5.373439
8 0.90309 2.33E-007 -5.039727
9 0.954243 1.04E-006 -4.745373
10
1 3.70E-006 -4.482065
11 1.041393 1.10E-005 -4.243873
12 1.079181 2.83E-005 -4.026421
13 1.113943 6.50E-005 -3.826384
14 1.146128 0.000136 -3.64118
15 1.176091 0.000261 -3.468758
16 1.20412 0.000471 -3.307468
17 1.230449
0.0008 -3.15596
18 1.255273 0.001293 -3.013114
19 1.278754 0.002001 -2.877994
20 1.30103 0.002982 -2.749805
21 1.322219 0.004296 -2.627873
22 1.342423 0.006009 -2.511614
23 1.361728 0.008186 -2.400523
24 1.380211 0.010891 -2.294162
25 1.39794 0.014185 -2.192143
26 1.414973 0.018124 -2.094125
27 1.431364 0.022761 -1.999808
28 1.447158 0.028136 -1.90892
29 1.462398 0.034286 -1.821223
30 1.477121 0.041238 -1.736499
31 1.491362 0.049008 -1.654553
32 1.50515 0.057604 -1.575209
33 1.518514 0.067027 -1.498307
34 1.531479 0.077267 -1.423701
35 1.544068 0.088306 -1.351258

m=
b=

5.75444
-10.2365
0.5

1.2
1
0.8
m (% )

0.6
0.4
0.2
0
1

1.2

36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80

1.556303
1.568202
1.579784
1.591065
1.60206
1.612784
1.623249
1.633468
1.643453
1.653213
1.662758
1.672098
1.681241
1.690196
1.69897
1.70757
1.716003
1.724276
1.732394
1.740363
1.748188
1.755875
1.763428
1.770852
1.778151
1.78533
1.792392
1.799341
1.80618
1.812913
1.819544
1.826075
1.832509
1.838849
1.845098
1.851258
1.857332
1.863323
1.869232
1.875061
1.880814
1.886491
1.892095
1.897627
1.90309

0.100122
0.112683
0.125952
0.139889
0.154447
0.169577
0.18523
0.20135
0.217885
0.234781
0.251982
0.269437
0.287091
0.304895
0.3228
0.340758
0.358726
0.376661
0.394523
0.412277
0.429887
0.447323
0.464555
0.481558
0.498309
0.514785
0.530969
0.546844
0.562397
0.577615
0.592489
0.60701
0.621171
0.634969
0.648399
0.66146
0.674151
0.686472
0.698425
0.710013
0.721238
0.732105
0.742619
0.752784
0.762607

-1.280855
-1.212382
-1.145735
-1.080819
-1.017546
-0.955837
-0.895614
-0.836808
-0.779355
-0.723192
-0.668264
-0.614518
-0.561903
-0.510373
-0.459884
-0.410394
-0.361866
-0.314262
-0.267549
-0.221692
-0.176661
-0.132428
-0.088964
-0.046243
-0.00424
0.037069
0.077706
0.117693
0.15705
0.195797
0.233952
0.271533
0.308558
0.345042
0.381001
0.416451
0.451404
0.485875
0.519877
0.553423
0.586524
0.619193
0.65144
0.683277
0.714713

81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125

1.908485
1.913814
1.919078
1.924279
1.929419
1.934498
1.939519
1.944483
1.94939
1.954243
1.959041
1.963788
1.968483
1.973128
1.977724
1.982271
1.986772
1.991226
1.995635
2
2.004321
2.0086
2.012837
2.017033
2.021189
2.025306
2.029384
2.033424
2.037426
2.041393
2.045323
2.049218
2.053078
2.056905
2.060698
2.064458
2.068186
2.071882
2.075547
2.079181
2.082785
2.08636
2.089905
2.093422
2.09691

0.772093
0.78125
0.790085
0.798604
0.806815
0.814727
0.822346
0.829681
0.836739
0.843529
0.850058
0.856334
0.862366
0.868161
0.873727
0.879071
0.884201
0.889125
0.893849
0.89838
0.902726
0.906893
0.910888
0.914718
0.918388
0.921904
0.925273
0.9285
0.931591
0.934551
0.937385
0.940098
0.942696
0.945182
0.947562
0.949839
0.952018
0.954104
0.956099
0.958008
0.959834
0.96158
0.963251
0.964849
0.966378

0.745758
0.776422
0.806715
0.836645
0.866221
0.895451
0.924343
0.952904
0.981143
1.009067
1.036682
1.063995
1.091013
1.117741
1.144187
1.170356
1.196254
1.221886
1.247258
1.272375
1.297243
1.321865
1.346247
1.370393
1.394308
1.417997
1.441463
1.46471
1.487744
1.510567
1.533184
1.555598
1.577812
1.599831
1.621658
1.643295
1.664747
1.686016
1.707106
1.728019
1.748759
1.769328
1.789729
1.809965
1.830038

126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157

2.100371
2.103804
2.10721
2.11059
2.113943
2.117271
2.120574
2.123852
2.127105
2.130334
2.133539
2.136721
2.139879
2.143015
2.146128
2.149219
2.152288
2.155336
2.158362
2.161368
2.164353
2.167317
2.170262
2.173186
2.176091
2.178977
2.181844
2.184691
2.187521
2.190332
2.193125
2.1959

0.96784
0.969238
0.970575
0.971853
0.973076
0.974245
0.975363
0.976432
0.977454
0.978431
0.979365
0.980259
0.981113
0.98193
0.982711
0.983457
0.984171
0.984854
0.985506
0.986131
0.986727
0.987298
0.987843
0.988365
0.988864
0.989341
0.989797
0.990233
0.99065
0.991049
0.99143
0.991795

1.849952
1.869708
1.889309
1.908757
1.928056
1.947206
1.966211
1.985072
2.003792
2.022373
2.040817
2.059126
2.077301
2.095346
2.113261
2.131048
2.14871
2.166247
2.183663
2.200958
2.218134
2.235193
2.252136
2.268965
2.285682
2.302288
2.318784
2.335171
2.351452
2.367628
2.383699
2.399668

%)

2
1.5

f(x) = 5.7544400391x
R = 0.9806792815

1
m (probit)

0.5
0
1.5
-0.5

1
0.8
0.6
0.4
0.2
0
1.4

1.8

log C (ng/kg)

1.2

1.2

1.7

-1

0
y = 5.7544x - 10.237
y=0
x = 10.237/5.7544
x=
1.778987
C = 10^ x 60.11551

1.6

1.6

1.8

log C (ng/kg)

2.2

2.4

f(x) = 5.7544400391x - 10.2365045872

R = 0.9806792815

1.6

1.7

1.8

log C (ng/kg)

1.9

2.1

sum (obs-predicted)^2 =
C (ng/kg)

M obs (%)
34
44
64
82
105
120
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
150
160
170
180
190
200
750

log C
M predict (%)
0 1.531479 6.0802312715
25 1.643453 17.2777961061
49 1.80618 53.3994433288
78 1.913814 77.9391119534
93 2.021189 91.5703481983
100 2.079181 95.2211072836
Err:502
0
1 0.0249181545
1.30103 0.5777025826
1.477121 3.5365195014
1.60206 11.9301082784
1.69897 27.1839127613
1.778151 46.0826861232
1.845098 63.2579973577
1.90309 75.9500266612
1.954243 84.3570325694
2 89.6941656705
2.041393 93.0648580266
2.079181 95.2211072836
2.113943 96.6288758042
2.146128 97.5691208498
2.176091 98.2115010237
2.20412 98.6599191064
2.230449 98.9792472951
2.255273 99.2108481104
2.278754 99.3816537907
2.30103 99.5095583413
2.875061 99.9987842045

140.8421741206
a=
(M obs - M predict)^2
36.9692123148
59.6324329794
19.3551016032
0.0037073542
2.043904274
22.837815595

4.543057

b=

62.10993

120
100
80
60
40
20
0
1

10

100

100

1000

sum (obs-predicted)^2 =
C (ng/kg)

M obs (%)
34
44
64
82
105
120
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
150
160
170
180
190
200

log C
M predict (%)
0 1.531479 5.1980964629
25 1.643453 17.0213646475
49 1.80618 54.517338697
78 1.913814 77.9621282783
93 2.021189 90.7552474733
100 2.079181 94.3375411024
Err:502
Err:502
1 0.0018964563
1.30103 0.2592315304
1.477121
2.71409729
1.60206 11.2939218389
1.69897 27.6290752612
1.778151 47.2219558197
1.845098 64.1017346461
1.90309 76.1041441628
1.954243 83.947415687
2 88.9639187907
2.041393 92.2017272548
2.079181 94.3375411024
2.113943 95.7833146604
2.146128 96.7877827546
2.176091 97.5030086606
2.20412 98.0238995278
2.230449 98.4110940341
2.255273 98.7042627965
2.278754 98.9299551717
2.30103 99.1063219883

158.2236441644
EC50 =
(M obs - M predict)^2
27.020206838
63.6586220883
30.4410262978
0.0014342673
5.0389139062
32.0634407668

1.788762

Hillcoefficient =

18.6973

120
100
80
60
40
20
0
0.8

1.2

1.4

61.48392

(Wikipedia EC50)
=0+(1000)/(1+
(/50)^() )

=100/(1+(/50)^() )

1.6

1.8

1.6

1.8

2.2

2.4