THE UNIVERSITY OF THE WEST INDIES EXAMINATION OF __pecemer 20.05, Code and Name of Course: BC 34C - Molecular Biology II Poper: x Dateand Time: Friday December 16, 2005 4;00pm - 6;00pm _ INSTRUCTIONS TO CANDIDATES: This paper has 1 page(s)and five (5) questions Answer Three (3) Questions. Answer Each Question in a Separate Booklet. 1, (a)Distinguish between a ‘pseudogene” and a ‘processed psuedogene’. [20 marks] (b)Using appropriate examples, describe the organisational differences between gene families and gene clusters. [30 marks] (Explain why the proteome of a unique cell is not necessarily representative of the number of unique genes in the species. [50 marks} 2. (a) Describe the mechanisms of homologous recombination, site-specific recombination and transposition. (90 marks}. (b) What are the salient differences between these recombination processes? [10 marks] 3. (@) Describe the DNA repair systems in the cell that are potentially mutagenic. [80 marks) (b) What are the consequences of these mutations? (20 marks} 4, Describe the following: a) The Britten-Davison’s model of regulation of gene activity. [25 marks] b) The relative roles of histone and non-histone proteins in the regulation of gene activity. [25 marks} ©) Hormonal control of gene expression. [25 marks] 4) Transcription factors. [25 marks} 5. (@)Explain what is RNA interference and why it is important. (20 marks] (b)Describe the mechanisms of gene silencing by double-stranded RNA. [80 marks]