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(Part 2)
Substance Properties
Checked
Substance Characteristics
Why LC50?
Response
100%
0%
Low
High
Lab hardiness
Common
Known life cycle
Cheap
Short-lived
Pure
Commercial formulation
Mixtures of known concentration
Carriers/solvents
Unknown mixtures (eg. sediment, effluent)
V1.N1 = V2.N2
x.1000 = 100.100
PA
PS
1000 g/mL
Diambil 10 mL
+ 90 mL medium
Original Concentration
PA
PS
V1.N1 = V2.N2
x.100 = 10.10
100 g/mL
Diambil 1 mL
+ 9 mL medium
Larutan Stock
10 g/mL
Diambil 1 mL
+ 9 mL medium
10 -1
V1.N1 = V2.N2
x.10 = 10.1
1 g/mL
Diambil 1 mL+
9 mL medium
10 -2
0,1 gL
10 -3
V1.N1 = V2.N2
x.1 10. (0,1)
1.
2.
3.
4.
10 -1
10 -2
Static Test
Renewable Test
Resirculation Test
Flow-Trough Test
10 -3
Observe Mortality
for Determine LC50
hewan
uji
5a
b
. (1)
b = XY 1/ n ( X Y)
X2 1/ n ( X)2
. (2)
a = 1/ n (Y b X)
..(3)
100
Frequency
Mean response
Protected
5% allowable
impact
Lihat di Tabel
Probit % Mortalitas
n
(jumlah
hewan uji)
30
ii
30
iii
30
r
(Mortalitas
hewan uji)
Jumlah ( )
p
(%Mortalitas)
X
(Log
Konsentrasi)
Y
(Nilai Probit %
Mortalitas)
XY
XY
Log
1.009
Probit
6.18
6.18
1.009
b = .. Persamaan (2)
a = . Persamaan (3)
m = . Persamaan (1)
Tolerance
Log of Concentration
Concentration
Dose-Response
Concentration
Log of Concentration
Cumulative distribution
# Responding
Normal distribution
Dose
Log Dose
0 (Kontrol)
30
0.251
30
0.63
30
1.58
30
13
3.97
30
19
9.96
30
30
LC_Cu_UdangWindu
Proportion
Observed Responding Predicted
Number Number Proportion Adjusted for Proportion
Conc. Exposed Resp. Responding Controls
Responding
0.2510
0.6300
1.5800
3.9700
9.9600
30
30
30
30
30
0
3
13
19
30
0.0000
0.1000
0.4333
0.6333
1.0000
0.0000
0.1000
0.4333
0.6333
1.0000
0.0083
0.0859
0.3674
0.7553
0.9572
=
=
0.330260
0.388480
Parameter
Estimate Std. Err.
95% Confidence Limits
--------------------------------------------------------------------Intercept
4.149867 0.176426 ( 3.804072, 4.495663)
Slope
2.574135 0.344351 ( 1.899208, 3.249063)
Theoretical Spontaneous Response Rate = 0.0000
LC_Cu_UdangWindu
Estimated LC/EC Values and Confidence Limits
Point
LC50 Value
LC/EC 1.00
LC/EC 5.00
LC/EC 10.00
LC/EC 15.00
LC/EC 50.00
LC/EC 85.00
LC/EC 90.00
LC/EC 95.00
LC/EC 99.00
Exposure
Conc.
0.267
0.491
0.680
0.847
2.139
5.406
6.732
9.317
17.139
0.122
0.274
0.419
0.555
1.665
4.007
4.837
6.355
10.490
0.431
0.712
0.936
1.132
2.762
8.407
11.154
17.060
38.270
LC_Cu_UdangWindu
EC99
Thank you
Photo by R. Grippo