y Bode Technology. 10430 Fumace Road, Suite 107 Lorton, VA 22079 Phone: 703-648-9740 Forensic Case Report ‘March 24, 2008, To: Andy Horta BODE Case #: 2507-101 Boulder Distt Attorney's Office ‘Agency Case #: S6DA21871 1777 6" Street Boulder, CO 80302 List of Evidence Received on December 3, 2007 for possible DNA analysis: BODE Sample# Agency Description 2807-101-01 Labeled as “Ligature from neck. BPD# O22TET, CBl# 008" 2807-107-02 Labeled as “Broken paintbrush handle (attached to #1 above)” 2807-101-03 Labeled as ‘Ligature from wrist, BPO # 018TET, CBI# 166° 2807-101-04 Labeled as Wednesday’ panties. BPD #021TET, CBI 7” 2807-101-05 Labeled as “White long underwear bottoms. 8PDG20TET, CBI# 6° 2807-101-05A ‘exterior top right half of long johns 207-101-058 ‘exterior top eft half of long johns 2807-101-056 interior top right half of long jonas 807-107-050 interior top left half of ong johns List of Evidence Received on January 23, 2008 for possible DNA analysis: BODE Sample # Agency Deseription 2807-101-06 Labeled as “Cutting from crotch of underwear. BPD# 110KKY" 2807-101-064 Cutting trom top layer 807-101-068 cutting from top tayer opposite of -06A 12807-107-08C ceutting from bottom layer same edge as -O6A Table One contains Profiler Plus and Cofller data submitted on March 12, 2008 for the following samples: BPD# cer Description oor aE Boos standard JonBienet-Victim 5RTG 368-2 Blood Standards Burke Ramsoy-Brother ARTGIOSSTET 358-2 Biood Standards Patricia Ramsey-Mother 3RTGIOSSTET 348-2 Blood Standards John B. Ramsey-Father 2RTGITZTET 398-2 Blood Standard Melinda Ramsey- % Sister (Common Father) sRTs. 328-2 Blood Standards John Andrew Ramsey- ‘Brother (Common Father) DNA PROCESSING, RESULTS, and CONCLUSIONS: ‘The evidence items were processed for DNA typing by analysis of the 13 CODIS Short Tandem Repeat loci, the D251338 focus, the 0198423 locus, and the gender determining focus Amelogenin using the AMpFLSTRE Identifier® kit. Appropriate positive and negative controls were used concurrently throughout the analysis. The ONA profiles reported in this case were determined dy procedures that have been validated according to standards established by tne Scientifie Working Group on DNA Analysis, Methods (SWGDAM) and adopted as Federal Standards. 41, The DNA profile obtained from sample 2S07:101-05A contains a mixture of at least two individuals including the victim and at least one male contsibutor. The profiles associated with the following individuals are excluded as possible contributors to the mixture DNA profile obtained from sample 2S07-101-08A: Burke Ramsey, Patricia Ramsey, John B. Ramsey, Melinds| Ramsey, and John Andrew Ramsey. . Page 1 of 4 1 erases)BODE Case #: 2807-101 Date: March 24, 2008 ‘Agency Caso #: 96DA21871 1 DNA PROCESSING, RESULTS, and CONCLUSIONS (cont) | 2, The partial ONA profile obtained from sample 207-101-058 contains a mixture of atleast two | individuals including the victim and at least one male contributor. The profiles associated with the following individuals are excluded as possiple contributors to tne mixture DNA profile obtained {rom sample 2S07-101-058: Jonn B, Ramsey, Melinda Ramsey, and John Andrew Ramsey. The Profiles associated with Burke Ramsey and Patricia Ramsey cannot be included or excluded from ‘the mixture DNA profile obtained from 2807-10-08, 3. The partial DNA profile obtained from sample 2807-101-05C contains a mixture of at least two indivicvals including a major component victim profile and at least one additional minor Contributor. The minor contributor is low level, contains allelic drop-out. and therefore is not suitable for comparison. 4. The DNA profile obtained from sample 207-101-06D contains a mixture of atleast three individuals including the victim and at least one male contributor. Due to the complexity ofthis ‘mixture it was deemed unsuitable for any further comparison purposes, 5. Samples 2507-101-05A, -06B, and -08C were combined and processed as -06X. The partial NA profile obtained from sample 2807-101-06X is consistent with the victim, ‘See Table Two for summary of alleles reported for these samples. Notes: 41. Ibis known that the vietim was wearing item 2S07-101-05 the night ofthe crime; therefore ite ‘expected that the victim would be present inthe samples associated with 2507-10-05, ‘Assuming the victim, JonBenet Ramsey is a contributor, the remaining DNA contribution is provided in Table Two for samples 2807-101-05A and 2S07-101-08B, Based on the results itis likely more than two people contributed to the mixtures observed in 2S07-101-05A and 2807- 101-058 therefore, the remaining DNA contribution should not be considered a single source profile, 2. Samples 2807-101-01, -02, -03, and -04 were net processed at this time 3. The DNA extracts and submitted evidence will be returned to Andy Horita at the Boulder District Attorney's Office, Report submitted by: Ang Wet ‘Amy Jeanguenat, MFS DNA Analyst i Page 2 of 134BODE Case #25 07-104 ‘Agency Case #: 960021671 vammey.| faa | ats, | ie Penang pret [20922 | 20902 | 2es22 | 2osee | se2ae2 | 2aa12 Oiess99 912 12,12 14,12 912 12,13 912 ‘THot 9.3.8.3 87 7.93 pace) ‘TOK 88 89 88 91 89 CSFIPO. 10,13 10,13 12,13 10,11 10,12 inc: inconciusive, no results ‘p= not performed “aint resuits Page 30f 4| rovetes at i: some pungent) estou; potsonn ato amy ea om) ‘SLinsay ON 08 SLINS3Y ON | SLINS3Y ON | SL1NS3Y ON ‘ONT Ezz Od un zit BAISMONOON| zh om stassa xx x AX xx : owy SLTNS3Y ON w+ ANSMONOON| art iss010 68 AISTIONOONI 6B XOdL ov9r a 8 vm se ‘cevsera SZ | BeErszo a6 FAISMTONOONI| ‘sesssLa aesesa e8e6 £6 Z Cy) ‘SL1NS34 ON ob ze'ee era bxe0-o-20st }49:2v096 s# seo fouaBy 002 "v2 YoveH :918q L0l-L082 %# e889 3008